FastQCFastQC Report
Thu 26 May 2016
SRR936275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097257
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11530.10508021366006323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.069.950661
TACGGTG359.157215E-567.992525
GTACATG87850.048.1478921
TACATGG87250.047.3198622
GTATCAA27800.045.153052
ACATGGG87050.044.3552633
TCAACGC30050.040.7842035
ATCAACG30750.039.855784
CAACGCA31100.039.789846
CATGGGG40150.039.5635224
AACGCAG31250.038.837337
CATGGGA33450.035.927294
GGAACGC1001.0629441E-535.696088
TATCAAC37150.033.6302223
CATGGGC17300.033.357614
CATGGGT17550.032.2044374
ATGGGGG17500.030.5966385
ACGCAGA38950.030.395898
CGCAGAG38150.030.2535539
CTCGCTC1004.4413636E-429.746738
Produced by FastQC (version 0.11.5)