##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1097257 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.642620644024145 31.0 30.0 34.0 25.0 34.0 2 29.582837931314177 31.0 30.0 34.0 24.0 34.0 3 29.796268330937966 31.0 30.0 34.0 25.0 34.0 4 33.0733146382297 35.0 35.0 37.0 25.0 37.0 5 33.48214775572177 37.0 35.0 37.0 28.0 37.0 6 33.325758687344894 37.0 35.0 37.0 27.0 37.0 7 33.23856124864093 37.0 35.0 37.0 26.0 37.0 8 33.16623270573803 37.0 35.0 37.0 26.0 37.0 9 34.34881071617679 39.0 35.0 39.0 22.0 39.0 10-11 34.32747569621337 39.0 35.0 39.0 25.0 39.0 12-13 34.36857955793401 39.0 35.0 39.0 25.0 39.0 14-15 35.51880461915485 39.5 36.0 41.0 25.5 41.0 16-17 35.6085479518472 39.0 36.0 41.0 25.5 41.0 18-19 35.70783143784911 39.5 36.0 41.0 26.0 41.0 20-21 35.898218010912665 40.0 36.0 41.0 27.0 41.0 22-23 35.848733705959496 39.5 35.0 41.0 27.0 41.0 24-25 35.88983984608893 39.0 35.0 41.0 27.0 41.0 26-27 36.03687741340452 39.0 35.0 41.0 27.5 41.0 28-29 35.969800602775834 39.0 35.0 41.0 27.5 41.0 30-31 35.903208181857124 39.0 35.0 41.0 27.5 41.0 32-33 35.76788300279698 39.0 34.5 41.0 27.0 41.0 34-35 35.74002717686012 39.0 35.0 41.0 27.0 41.0 36-37 35.630922837585 39.0 35.0 41.0 27.0 41.0 38-39 35.39247414233857 39.0 34.0 41.0 26.5 41.0 40-41 35.500650257870305 39.0 34.5 41.0 27.0 41.0 42-43 35.43899514881199 39.0 34.0 41.0 27.0 41.0 44-45 35.371308635989564 39.0 34.0 41.0 27.0 41.0 46-47 35.394523798891235 39.0 34.0 41.0 27.0 41.0 48-49 35.34768563791345 39.0 34.0 41.0 27.0 41.0 50-51 35.271593163679974 39.0 34.0 41.0 27.0 41.0 52-53 35.19472420772891 39.0 34.0 40.5 27.0 41.0 54-55 35.108451802996015 39.0 34.0 40.0 27.0 41.0 56-57 34.97809674488292 39.0 33.5 40.0 26.5 41.0 58-59 34.81243136293503 38.5 33.0 40.0 26.0 41.0 60-61 34.650743171380995 38.0 33.0 40.0 26.0 41.0 62-63 34.47066867652701 38.0 33.0 40.0 26.0 41.0 64-65 34.2531858990191 38.0 33.0 40.0 25.0 41.0 66-67 34.006851175248826 38.0 32.5 40.0 24.0 41.0 68-69 33.76883264358304 37.5 32.0 40.0 23.0 41.0 70-71 33.44669571485987 37.0 32.0 40.0 22.5 41.0 72-73 33.140478939756136 36.5 32.0 39.0 22.0 41.0 74-75 32.808047704412004 36.0 31.5 39.0 22.0 40.5 76-77 32.451998027809346 36.0 31.0 39.0 20.5 40.0 78-79 32.061670602238124 35.0 31.0 38.0 19.5 40.0 80-81 31.67495172051762 35.0 31.0 37.0 18.0 39.0 82-83 31.288196839938138 35.0 31.0 37.0 15.5 39.0 84-85 31.17530624092624 35.0 31.0 36.5 16.0 39.0 86-87 30.934277475559508 35.0 31.0 36.0 12.5 37.0 88-89 30.65229659049794 35.0 31.0 36.0 8.5 37.0 90-91 30.39213374806449 35.0 31.0 35.5 7.0 37.0 92-93 30.195033159961614 35.0 31.0 35.0 4.0 36.0 94-95 30.015217036665067 34.0 31.0 35.0 2.0 36.0 96-97 29.838222494821174 34.0 30.0 35.0 2.0 36.0 98-99 29.698881392417636 34.0 30.0 35.0 2.0 36.0 100-101 29.59253301642186 34.0 30.0 35.0 2.0 35.5 102-103 28.663732379925577 33.5 28.5 34.5 2.0 35.0 104-105 29.287008421910272 34.0 29.5 35.0 2.0 35.0 106-107 29.3879783861028 34.0 30.0 35.0 2.0 35.0 108-109 29.338176015281743 34.0 30.0 35.0 2.0 35.0 110-111 29.251832524194423 34.0 30.0 35.0 2.0 35.0 112-113 29.23134142684895 34.0 30.0 35.0 2.0 35.0 114-115 29.131718457936472 34.0 30.0 35.0 2.0 35.0 116-117 29.01442642881294 34.0 30.0 35.0 2.0 35.0 118-119 28.836546041629262 34.0 29.0 35.0 2.0 35.0 120-121 28.674325613780546 34.0 29.0 35.0 2.0 35.0 122-123 28.42633995499687 34.0 29.0 35.0 2.0 35.0 124-125 26.555949973433755 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4053319750279236 1101 2 0.2978850084811526 1101 3 0.33770418453685025 1101 4 0.3668024503734415 1101 5 0.3618710007472927 1101 6 0.22819379955521413 1101 7 0.24096678934982663 1101 8 0.301794768764438 1101 9 0.29229284777851205 1101 10-11 0.40917046175627547 1101 12-13 0.4815792799227623 1101 14-15 0.3066896323699879 1101 16-17 0.23689691397285628 1101 18-19 0.18347246427187258 1101 20-21 0.22114431877260898 1101 22-23 0.33484468409400847 1101 24-25 0.4119981320577111 1101 26-27 0.22467827470584467 1101 28-29 0.43319556396415493 1101 30-31 0.4816521675511254 1101 32-33 0.5071245058175009 1101 34-35 0.38453122799732853 1101 36-37 0.2807009712951114 1101 38-39 0.5220022922003409 1101 40-41 0.4806688706459141 1101 42-43 0.48075248991678166 1101 44-45 0.5573986727844726 1101 46-47 0.5608095605237651 1101 48-49 0.5364905334611976 1101 50-51 0.5703512688073005 1101 52-53 0.4088514750846386 1101 54-55 0.4057632632718864 1101 56-57 0.46364269122409496 1101 58-59 0.4065184034812219 1101 60-61 0.4670561677476712 1101 62-63 0.4888531917145471 1101 64-65 0.5366252291107614 1101 66-67 0.4030084944104715 1101 68-69 0.2738116242830344 1101 70-71 0.30613247788590314 1101 72-73 0.3604596397631994 1101 74-75 0.2085220481308383 1101 76-77 0.15209070081986198 1101 78-79 0.3426234954741858 1101 80-81 0.35448936404414155 1101 82-83 0.24111582962694555 1101 84-85 0.1686989653116342 1101 86-87 0.29467951431339046 1101 88-89 0.3284185858747968 1101 90-91 0.24663393698906333 1101 92-93 0.37075983966366977 1101 94-95 0.4752242219468812 1101 96-97 0.47593043247089284 1101 98-99 0.5214030389201803 1101 100-101 0.6184755035064775 1101 102-103 0.6445036317537607 1101 104-105 0.5866527516217843 1101 106-107 0.6311636366105162 1101 108-109 0.7732305585790016 1101 110-111 0.6333917926109471 1101 112-113 0.7087616137459385 1101 114-115 0.7385771984401792 1101 116-117 0.7044622485612919 1101 118-119 0.7752487922032749 1101 120-121 0.769079293590611 1101 122-123 0.7804034624038145 1101 124-125 0.40176152839939405 1102 1 0.38594832995192974 1102 2 0.3958515720117184 1102 3 0.4703985409124023 1102 4 0.43641401350080145 1102 5 0.4348323650299477 1102 6 0.36104519443915706 1102 7 0.3858793783206309 1102 8 0.36705381863902176 1102 9 0.47886838966747547 1102 10-11 0.5083731730048981 1102 12-13 0.31330562122261796 1102 14-15 0.6217258573340132 1102 16-17 0.5405329920846569 1102 18-19 0.6175046189675442 1102 20-21 0.49598346040860974 1102 22-23 0.7251782211529729 1102 24-25 0.7986715046931181 1102 26-27 0.6624034870261468 1102 28-29 0.6447731218880222 1102 30-31 0.7085420411139083 1102 32-33 0.6889268198011536 1102 34-35 0.7020965156961267 1102 36-37 0.7423193804525781 1102 38-39 0.6993327224434083 1102 40-41 0.5470245374017111 1102 42-43 0.6713622237577397 1102 44-45 0.7385669968440425 1102 46-47 0.6492089773591445 1102 48-49 0.7463671945678456 1102 50-51 0.6230132837386932 1102 52-53 0.5970228677615523 1102 54-55 0.502993443526627 1102 56-57 0.5736736320545361 1102 58-59 0.7306824917453554 1102 60-61 0.7839688967798466 1102 62-63 0.6019097765089754 1102 64-65 0.6273961396370069 1102 66-67 0.4836164668735563 1102 68-69 0.382380939807355 1102 70-71 0.4734713330327409 1102 72-73 0.5092976609082243 1102 74-75 0.45152163219101027 1102 76-77 0.5830844786994973 1102 78-79 0.3127707933507722 1102 80-81 0.3541705122078582 1102 82-83 0.23724132886085414 1102 84-85 0.39782239333494473 1102 86-87 0.25936070491993846 1102 88-89 0.28054995919777426 1102 90-91 0.40454676788405664 1102 92-93 0.3378608026917149 1102 94-95 0.27574340398846786 1102 96-97 0.33597304508235837 1102 98-99 0.32432607731822216 1102 100-101 0.4452323187655267 1102 102-103 0.46071234017569296 1102 104-105 0.46956356648187025 1102 106-107 0.49875417779917086 1102 108-109 0.4091696700805869 1102 110-111 0.3824373592440864 1102 112-113 0.4835206445212421 1102 114-115 0.4923710578100753 1102 116-117 0.40165433636703796 1102 118-119 0.5127923491679347 1102 120-121 0.5784870674212073 1102 122-123 0.41283785638372095 1102 124-125 0.4221229504043116 1103 1 0.25103409737711146 1103 2 0.2495581701092604 1103 3 0.28271717445973366 1103 4 0.3512390053778276 1103 5 0.19006048061149272 1103 6 0.3574066612806064 1103 7 0.22689732729713796 1103 8 0.2848717071133535 1103 9 0.32926697570939467 1103 10-11 0.5323499580989477 1103 12-13 0.27029166090755297 1103 14-15 0.42426190654244067 1103 16-17 0.33093009358264425 1103 18-19 0.42080604861136806 1103 20-21 0.3358326811630903 1103 22-23 0.4455153526779654 1103 24-25 0.6187734900605477 1103 26-27 0.4887475594942998 1103 28-29 0.40695478524947504 1103 30-31 0.47189873500655466 1103 32-33 0.5781119172208449 1103 34-35 0.45972441055680235 1103 36-37 0.44377059217655557 1103 38-39 0.5494575081648989 1103 40-41 0.6689490105286637 1103 42-43 0.7288679938719014 1103 44-45 0.8277418705177553 1103 46-47 0.6997093807069277 1103 48-49 0.5182495122490423 1103 50-51 0.39026410443939596 1103 52-53 0.5130480994064257 1103 54-55 0.5865697658911415 1103 56-57 0.5602329073730132 1103 58-59 0.6825496558742827 1103 60-61 0.6618112382137511 1103 62-63 0.6438537447789514 1103 64-65 0.5412498756458817 1103 66-67 0.5085322960333514 1103 68-69 0.5628797745804164 1103 70-71 0.6487000368298013 1103 72-73 0.6359103517981666 1103 74-75 0.43832739782353514 1103 76-77 0.4543815219361349 1103 78-79 0.36555333287325453 1103 80-81 0.3552321011740531 1103 82-83 0.44914036866904183 1103 84-85 0.36399930055374696 1103 86-87 0.29067617890961017 1103 88-89 0.4700092770918509 1103 90-91 0.5335529357439555 1103 92-93 0.4816318804145894 1103 94-95 0.5448793153864031 1103 96-97 0.5056010968005076 1103 98-99 0.5495930151242341 1103 100-101 0.4836112808172217 1103 102-103 0.5368932528621038 1103 104-105 0.6005899745577885 1103 106-107 0.5107019554649099 1103 108-109 0.39916580466429963 1103 110-111 0.5199593473005066 1103 112-113 0.5036208092215873 1103 114-115 0.49891985729020405 1103 116-117 0.6366737474792714 1103 118-119 0.63805354487004 1103 120-121 0.4541053939760573 1103 122-123 0.5448145126305697 1103 124-125 0.4054197303444802 1104 1 0.24225303060153536 1104 2 0.2584745779717892 1104 3 0.35643489475390666 1104 4 0.34238427193466237 1104 5 0.1997937318950065 1104 6 0.1722436078212226 1104 7 -0.04639607299176873 1104 8 0.07667874019003307 1104 9 0.2642220493212406 1104 10-11 0.19309062675930022 1104 12-13 0.024841177243054346 1104 14-15 0.09292431173516036 1104 16-17 0.21786940458344617 1104 18-19 0.16312432836105017 1104 20-21 0.01946192607631758 1104 22-23 0.1320787580927032 1104 24-25 0.17652147604812285 1104 26-27 0.10512393380594887 1104 28-29 0.08132344477652964 1104 30-31 0.17294260881794088 1104 32-33 0.337864870054986 1104 34-35 0.2311005929924761 1104 36-37 0.22130479406845183 1104 38-39 0.4646748830823242 1104 40-41 0.1905446511065918 1104 42-43 0.3131955987937971 1104 44-45 0.3375395373059433 1104 46-47 0.18028862000093682 1104 48-49 0.25241374848673814 1104 50-51 0.202840169556616 1104 52-53 0.13949367926931444 1104 54-55 0.09186811246848947 1104 56-57 0.09427136205876252 1104 58-59 0.12175889387998495 1104 60-61 0.148555178359409 1104 62-63 0.056341170050110634 1104 64-65 0.1414723489819707 1104 66-67 0.2930232011123195 1104 68-69 0.125117315662429 1104 70-71 0.17039384252523604 1104 72-73 0.1465558705348542 1104 74-75 0.1948066382139757 1104 76-77 0.12136843873597769 1104 78-79 -0.02312947969955559 1104 80-81 0.20946221254434505 1104 82-83 0.16829013882903965 1104 84-85 0.16834799812706436 1104 86-87 0.2212115951022966 1104 88-89 0.13525337759100253 1104 90-91 0.07477389051879157 1104 92-93 -6.103410705833312E-4 1104 94-95 0.08254611042723425 1104 96-97 -0.012897631335910376 1104 98-99 -0.004660601052556501 1104 100-101 -0.016088741659675776 1104 102-103 0.054414612769345894 1104 104-105 -0.1240433367554914 1104 106-107 -0.02809810703183757 1104 108-109 0.06775865481738919 1104 110-111 0.12830588352806416 1104 112-113 -0.03648743623711681 1104 114-115 -0.01175153043905297 1104 116-117 0.0356375144431631 1104 118-119 -0.026726626941186993 1104 120-121 0.08042197151176467 1104 122-123 -0.010128315182672765 1104 124-125 -0.15809447121848663 1105 1 0.024352177673456765 1105 2 0.17362119783710384 1105 3 0.26618805750162267 1105 4 0.08084215404046091 1105 5 0.15672850915646563 1105 6 0.1378854976917836 1105 7 0.10791410563782478 1105 8 0.12721522729638934 1105 9 0.19169956273341882 1105 10-11 0.24764112768004054 1105 12-13 0.15341526498229285 1105 14-15 0.20754757413519087 1105 16-17 0.16680673464600204 1105 18-19 0.036944987435788335 1105 20-21 0.011508853740309632 1105 22-23 -0.14502231687588818 1105 24-25 0.12425436928918288 1105 26-27 0.14261869856931497 1105 28-29 0.22595026381351602 1105 30-31 0.23724710347634215 1105 32-33 0.36143639834521224 1105 34-35 0.2523777580536688 1105 36-37 0.2816321708658833 1105 38-39 0.2983972859063613 1105 40-41 0.09460952150539725 1105 42-43 0.23783528155507838 1105 44-45 0.39182777780108324 1105 46-47 0.23631462666214986 1105 48-49 0.2307072940216628 1105 50-51 0.03483361470795643 1105 52-53 0.2214440791416763 1105 54-55 0.1834980493797289 1105 56-57 0.13416519478381161 1105 58-59 0.20365876956388718 1105 60-61 0.3227192024780621 1105 62-63 0.3348884999132338 1105 64-65 0.3383034519383017 1105 66-67 0.20563081430976382 1105 68-69 0.30899910235728356 1105 70-71 0.22954453756522497 1105 72-73 0.22586854213427188 1105 74-75 0.3554080754941751 1105 76-77 0.3330885546756974 1105 78-79 0.39141267808216895 1105 80-81 0.35962824433470075 1105 82-83 0.30377756428923774 1105 84-85 0.34128248952798046 1105 86-87 0.4468168881758281 1105 88-89 0.4051545895067328 1105 90-91 0.34239220686669114 1105 92-93 0.3638797967407861 1105 94-95 0.38343477107088475 1105 96-97 0.342877748758891 1105 98-99 0.4044944151351295 1105 100-101 0.4303448157085441 1105 102-103 0.29684994513208096 1105 104-105 0.29394809407623157 1105 106-107 0.30420579596172104 1105 108-109 0.36384685276583895 1105 110-111 0.3659744913872345 1105 112-113 0.3449293018946804 1105 114-115 0.4011914702988655 1105 116-117 0.35679618033866944 1105 118-119 0.5091769582818166 1105 120-121 0.42789808886083947 1105 122-123 0.3508728282594866 1105 124-125 0.5194450340433399 1106 1 -0.09197673076526058 1106 2 -0.34612325795322363 1106 3 -0.03193725341922615 1106 4 0.04427655301409317 1106 5 0.001346632896414235 1106 6 -0.20543003133185778 1106 7 -0.18371786880775431 1106 8 -0.14457803324873453 1106 9 0.0047425287219553525 1106 10-11 -0.046048815503269225 1106 12-13 -0.20251383197264516 1106 14-15 -0.21306549632274852 1106 16-17 -0.07424201774318107 1106 18-19 -0.13424083195519643 1106 20-21 -0.24385011256241285 1106 22-23 -0.3291300067667038 1106 24-25 -4.039776343145032 1106 26-27 -1.158155003417967 1106 28-29 -0.739339841703206 1106 30-31 -0.6386889319228146 1106 32-33 -5.421614815227954 1106 34-35 -1.114825001614335 1106 36-37 -0.34583228597175975 1106 38-39 -0.03282571307251203 1106 40-41 -0.5654106392570384 1106 42-43 -0.19174474619869386 1106 44-45 -0.21071260918082402 1106 46-47 -0.13918219212663274 1106 48-49 -0.008756743891041197 1106 50-51 -0.10956653715232534 1106 52-53 0.0019149769501751734 1106 54-55 -0.16854944623464974 1106 56-57 -0.1506959054177912 1106 58-59 0.006292760085713667 1106 60-61 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-0.3346007788896088 2216 12-13 -0.5210416579323933 2216 14-15 -0.7411474590821427 2216 16-17 -0.6956906290574665 2216 18-19 -0.36738447054164425 2216 20-21 -0.44533035853428515 2216 22-23 -0.35055458514542437 2216 24-25 -0.4632654029347947 2216 26-27 -0.4289350027910146 2216 28-29 -0.5956594588480897 2216 30-31 -0.3715034237829613 2216 32-33 -0.2538930069062886 2216 34-35 -0.5243292439499214 2216 36-37 -0.6468121505513906 2216 38-39 -0.3959300630338518 2216 40-41 -0.6195526634571635 2216 42-43 -0.22852689286230543 2216 44-45 -0.3281494182339202 2216 46-47 -0.5025575656820465 2216 48-49 -0.7153217843290705 2216 50-51 -0.7362481895733808 2216 52-53 -0.9122991983021649 2216 54-55 -0.7940242273259557 2216 56-57 -0.9011295431683095 2216 58-59 -0.8890268257118663 2216 60-61 -1.2701436837393771 2216 62-63 -1.2693163930531313 2216 64-65 -1.0601648597773945 2216 66-67 -0.986998759664715 2216 68-69 -1.0037236646995567 2216 70-71 -1.1556678161203209 2216 72-73 -1.2260170689592584 2216 74-75 -1.0857293546439628 2216 76-77 -0.9331095102826517 2216 78-79 -0.81782556403531 2216 80-81 -0.9682466370319602 2216 82-83 -0.9788782408357228 2216 84-85 -1.251997590615435 2216 86-87 -1.1338083095464135 2216 88-89 -1.0092982471483332 2216 90-91 -0.8904138671605182 2216 92-93 -0.9553803266378935 2216 94-95 -0.9142901050231806 2216 96-97 -1.0632918441969252 2216 98-99 -1.0393537869106382 2216 100-101 -0.9616988413620007 2216 102-103 -1.033102996974847 2216 104-105 -0.9333856874275135 2216 106-107 -0.9193547861325513 2216 108-109 -0.9827312343373968 2216 110-111 -1.0383094904750472 2216 112-113 -0.9743795929698109 2216 114-115 -0.8351558090431155 2216 116-117 -0.8031999970313635 2216 118-119 -0.8720882004232351 2216 120-121 -0.9404742616611443 2216 122-123 -0.8928363091442897 2216 124-125 -0.8975241476502163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23674.0 3 3961.0 4 2970.0 5 2973.0 6 3736.0 7 3255.0 8 2906.0 9 3176.0 10 3981.0 11 4961.0 12 5908.0 13 6689.0 14 7155.0 15 7125.0 16 6621.0 17 5945.0 18 5216.0 19 4669.0 20 4354.0 21 3981.0 22 4324.0 23 5737.0 24 8859.0 25 14590.0 26 19632.0 27 16186.0 28 15965.0 29 20513.0 30 23922.0 31 27841.0 32 36151.0 33 47949.0 34 64927.0 35 92835.0 36 151599.0 37 275492.0 38 156653.0 39 826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.92444467937542 20.782611786385772 10.912472920914032 21.38047061332478 2 16.604724156849734 23.087999679199164 41.1582003802219 19.1490757837292 3 16.200321529342286 31.69151045881727 31.73361549173575 20.374552520104697 4 9.50696651987518 19.274494006824348 40.79870731138266 30.419832161917814 5 9.266423693609699 44.1647732813484 36.986951818242304 9.5818512067996 6 25.946644502043302 41.354520963432016 21.05192646302919 11.646908071495492 7 23.76731842200718 36.81074913282576 24.741852754451614 14.680079690715447 8 23.23098838263748 38.236224261485894 22.194475760478365 16.33831159539826 9 25.873585416379964 15.596168901534863 21.34637100118389 37.18387468090128 10-11 21.242101694359153 29.823947416628616 31.533982422994654 17.399968466017576 12-13 22.204850066805076 29.425175579782398 30.90067461279486 17.469299740617668 14-15 20.125601016036665 31.285690133425447 27.648264293484033 20.94044455705386 16-17 19.076816373104975 32.69002218345737 29.174755334875417 19.05840610856223 18-19 18.487969752468413 31.82151878371894 30.617559845918773 19.072951617893874 20-21 20.717553773240976 30.878919066715277 30.084077652205615 18.319449507838133 22-23 20.068127435158974 31.28789862258149 29.698197651665193 18.945776290594345 24-25 19.593177611234303 31.168252961372062 30.383217084043086 18.85535234335055 26-27 18.970448176080566 31.296634692064067 30.445624188293195 19.287292943562168 28-29 19.033473003547577 31.12540471324315 30.184305442581415 19.656816840627855 30-31 19.410882809972342 31.475390413174935 30.03800462070568 19.075722156147044 32-33 18.99319601412473 31.48462324711123 30.35591166620869 19.16626907255534 34-35 19.059649237928454 31.843321353268816 30.469282230543104 18.627747178259625 36-37 18.899243104320366 31.617642367930586 30.1588068298329 19.324307697916144 38-39 19.07792026699792 31.518213476213436 30.26114686252833 19.14271939426031 40-41 19.467423965556996 31.216660454069462 30.62157262860304 18.694342951770498 42-43 18.836917659089107 31.73483979216774 30.633795435965627 18.79444711277753 44-45 18.558381454423493 32.08715203348061 30.29090474940578 19.063561762690114 46-47 18.79208913395156 31.6421426781198 30.032718904509103 19.533049283419533 48-49 19.060671140572605 31.477332225109738 30.21089513206407 19.251101502253583 50-51 18.889433187587862 31.77398591456232 30.01574415632056 19.320836741529256 52-53 18.654589631746738 31.848675852188318 30.244688006386973 19.25204650967797 54-55 18.88593296586319 31.666821601994837 30.343263776484825 19.103981655657147 56-57 18.99812893659255 31.839611679154096 29.730131229624956 19.432128154628398 58-59 18.93187403479368 31.8834675565362 29.789303064645413 19.395355344024708 60-61 18.990267407069187 31.779845995547014 29.732711409799673 19.49717518758413 62-63 18.863452741002273 31.53712215836983 30.506304252824485 19.09312084780341 64-65 18.91389155593793 31.94378432765529 30.162767711851163 18.97955640455562 66-67 18.750458532486427 32.37875034005453 29.695953658713304 19.174837468745743 68-69 19.014894468276687 32.0242915717706 29.949428254012524 19.0113857059402 70-71 19.44097363674724 31.49464067798773 30.199353472827905 18.86503221243713 72-73 19.150214991241775 31.519684594451242 30.168538916239996 19.16156149806699 74-75 18.837275005696057 32.07245386192755 30.19744816586922 18.89282296650718 76-77 18.865180100414765 31.83138011517896 30.288794977256757 19.014644807149516 78-79 18.73294317665549 31.686081085330027 30.27984012781026 19.301135610204224 80-81 18.898305895712586 31.978001296882695 29.87264028638961 19.251052521015104 82-83 19.042981922110876 31.789552445564006 30.033802626926175 19.133663005398944 84-85 19.00699795898559 31.55816572089185 30.36153615156457 19.07330016855799 86-87 18.498362039228127 32.05185516316496 30.091178069507503 19.358604728099422 88-89 18.839670320807002 32.007562553058854 29.823326256540795 19.329440869593352 90-91 18.901717430721092 31.77545307607796 30.015142408358354 19.307687084842602 92-93 18.739576213260424 31.78382319434951 30.18514467988152 19.291455912508543 94-95 18.622462115429748 31.828871790548625 30.327205120084578 19.221460973937056 96-97 18.79563124802346 31.685390800795084 29.946402223018154 19.5725757281633 98-99 18.768327109426085 31.851792781482757 29.874572509848505 19.50530759924265 100-101 18.894569269530592 31.61923961479941 30.005336084462336 19.480855031207664 102-103 18.552148368701637 32.310767157033624 29.740635476575534 19.396448997689202 104-105 18.313341623782804 32.18912067616529 29.74299758531925 19.754540114732652 106-107 18.576289444796082 31.8451978158033 29.609007282326516 19.9695054570741 108-109 18.48212430416571 32.46465690402499 29.378697901287225 19.67452089052207 110-111 18.467439270461757 32.409577832419835 29.516186948731455 19.606795948386956 112-113 18.470744026407935 32.46810651394491 29.426126866719038 19.635022592928113 114-115 18.46361849246341 32.38354718673189 29.097413800174436 20.055420520630268 116-117 18.562541353684967 32.33311338793083 28.87339347199615 20.23095178638805 118-119 18.555186445929998 32.10911974189668 29.17930069675138 20.15639311542195 120-121 18.866140469275962 31.622561446023607 29.573882187499855 19.937415897200577 122-123 18.876120022583866 31.707045883616537 29.52229837770741 19.89453571609219 124-125 18.867492916362497 31.806101998911824 29.0851558491388 20.241249235586885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 621.0 1 808.0 2 1001.5 3 869.5 4 584.0 5 394.0 6 313.5 7 274.5 8 257.5 9 274.5 10 266.0 11 212.5 12 226.0 13 274.0 14 278.0 15 305.5 16 398.0 17 560.0 18 785.5 19 1097.5 20 1596.5 21 2283.5 22 3280.5 23 4755.5 24 6525.0 25 8716.0 26 11752.0 27 15210.0 28 18930.0 29 23641.5 30 29796.5 31 36124.5 32 43242.0 33 51136.5 34 57614.5 35 61279.0 36 62158.0 37 61081.0 38 57953.5 39 54249.5 40 50069.5 41 46057.5 42 41721.0 43 37612.0 44 34823.0 45 32399.5 46 29842.5 47 27069.5 48 24701.5 49 22347.5 50 20355.0 51 18110.5 52 16028.0 53 14051.5 54 11864.0 55 10053.5 56 8327.5 57 6654.5 58 5191.0 59 4060.5 60 3203.0 61 2546.0 62 2012.0 63 1455.0 64 1088.5 65 867.5 66 654.0 67 591.0 68 494.5 69 388.0 70 327.0 71 258.5 72 213.5 73 207.0 74 206.0 75 167.5 76 103.0 77 76.5 78 56.5 79 44.5 80 26.0 81 16.5 82 14.5 83 17.0 84 13.5 85 10.5 86 8.5 87 6.0 88 3.5 89 2.0 90 1.5 91 1.5 92 2.0 93 3.5 94 4.5 95 2.5 96 2.0 97 2.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.290908146405081E-4 2 2.734090554901905E-4 3 9.113635183006351E-5 4 8.202271664705716E-4 5 0.0014581816292810162 6 0.0011847725737908256 7 0.0013670452774509527 8 0.0018227270366012703 9 0.002369545147581651 10-11 0.0025518178512417785 12-13 0.003189772314052223 14-15 0.004784658471078335 16-17 0.003918863128692732 18-19 0.003964431304607763 20-21 0.00519477205431362 22-23 0.09806271456914833 24-25 0.004192272184182922 26-27 0.0029163632585620324 28-29 0.0026885223789868736 30-31 0.0020049997402613973 32-33 0.0029619314344770642 34-35 0.0015948861570261115 36-37 0.001093636221960762 38-39 0.0018227270366012703 40-41 0.002187272443921524 42-43 0.002369545147581651 44-45 0.002278408795751588 46-47 0.0029163632585620324 48-49 5.012499350653493E-4 50-51 0.002232840619836556 52-53 0.002096136092091461 54-55 0.002278408795751588 56-57 0.0018227270366012703 58-59 0.0015948861570261115 60-61 0.0014581816292810162 62-63 0.001959431564346366 64-65 0.001686022508856175 66-67 5.923862868954128E-4 68-69 3.6454540732025405E-4 70-71 9.113635183006352E-4 72-73 2.734090554901905E-4 74-75 6.379544628104446E-4 76-77 7.290908146405081E-4 78-79 5.012499350653493E-4 80-81 9.569316942156669E-4 82-83 4.556817591503176E-4 84-85 7.746589905555398E-4 86-87 7.746589905555398E-4 88-89 7.746589905555398E-4 90-91 6.835226387254764E-4 92-93 6.379544628104446E-4 94-95 8.657953423856034E-4 96-97 9.113635183006352E-4 98-99 8.657953423856034E-4 100-101 9.569316942156669E-4 102-103 0.0015948861570261115 104-105 9.569316942156669E-4 106-107 0.0010480680460457305 108-109 0.0011847725737908256 110-111 0.0013670452774509527 112-113 0.0010024998701306986 114-115 9.113635183006352E-4 116-117 0.0013670452774509527 118-119 0.0020049997402613973 120-121 0.0012303407497058574 122-123 0.0012303407497058574 124-125 9.113635183006352E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1097257.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.82410874444351 #Duplication Level Percentage of deduplicated Percentage of total 1 53.150060093634565 20.103536527553413 2 14.24698543578701 10.777590528074217 3 10.243760583945262 11.62383342837569 4 7.179619466260463 10.86250829742237 5 4.830053571982072 9.134623577406883 6 3.242389650135834 7.3584299231157555 7 2.098657943834124 5.556590639948049 8 1.3688806640078897 4.142135287488037 9 0.9403180538051312 3.2010023089339894 >10 2.627042171235625 14.2937844217328 >50 0.05033032741784339 1.2929387121445508 >100 0.020457948083278422 1.280508315806887 >500 0.0012034082258083499 0.2675538846627713 >1k 2.4068164516166993E-4 0.10496414733452716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1153 0.10508021366006323 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.113635183006351E-5 0.0 10-11 0.0 0.0 0.0 9.113635183006351E-5 0.0 12-13 0.0 0.0 0.0 9.113635183006351E-5 0.0 14-15 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 16-17 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 18-19 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 20-21 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 22-23 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 24-25 0.0 0.0 0.0 2.734090554901905E-4 1.8227270366012703E-4 26-27 0.0 0.0 0.0 4.1011358323528584E-4 1.8227270366012703E-4 28-29 0.0 0.0 0.0 6.835226387254764E-4 1.8227270366012703E-4 30-31 0.0 0.0 0.0 0.0026885223789868736 1.8227270366012703E-4 32-33 0.0 0.0 0.0 0.007245339970490049 1.8227270366012703E-4 34-35 0.0 0.0 0.0 0.013123634663529147 1.8227270366012703E-4 36-37 0.0 0.0 0.0 0.02237397437428059 1.8227270366012703E-4 38-39 0.0 0.0 0.0 0.039963290277482855 1.8227270366012703E-4 40-41 0.0 0.0 0.0 0.06115249207797262 1.8227270366012703E-4 42-43 0.0 0.0 0.0 0.08667067059039041 1.8227270366012703E-4 44-45 0.0 0.0 0.0 0.11865953008274269 1.8227270366012703E-4 46-47 0.0 0.0 0.0 0.15520520716659816 1.8227270366012703E-4 48-49 0.0 0.0 0.0 0.1985861106377084 1.8227270366012703E-4 50-51 0.0 0.0 0.0 0.24255940039571403 1.8227270366012703E-4 52-53 0.0 0.0 0.0 0.2882187126625758 1.8227270366012703E-4 54-55 0.0 0.0 0.0 0.34403972815848977 1.8227270366012703E-4 56-57 0.0 0.0 0.0 0.4142602872435537 1.8227270366012703E-4 58-59 0.0 0.0 0.0 0.5002930033711337 1.8227270366012703E-4 60-61 0.0 0.0 0.0 0.5884218555908051 1.8227270366012703E-4 62-63 0.0 0.0 0.0 0.6893553652426004 1.8227270366012703E-4 64-65 0.0 0.0 0.0 0.799356941901487 1.8227270366012703E-4 66-67 0.0 0.0 0.0 0.9339197653785758 1.8227270366012703E-4 68-69 0.0 0.0 0.0 1.0894439497765793 1.8227270366012703E-4 70-71 0.0 0.0 0.0 1.26278529095736 1.8227270366012703E-4 72-73 0.0 0.0 0.0 1.4344861778051996 1.8227270366012703E-4 74-75 0.0 0.0 0.0 1.6237308123803267 1.8227270366012703E-4 76-77 0.0 0.0 0.0 1.8390404435788517 1.8227270366012703E-4 78-79 0.0 0.0 0.0 2.102606773071395 1.8227270366012703E-4 80-81 0.0 0.0 0.0 2.3741475333490696 1.8227270366012703E-4 82-83 0.0 0.0 0.0 2.6559411332076257 1.8227270366012703E-4 84-85 0.0 0.0 0.0 2.9582859804038613 1.8227270366012703E-4 86-87 0.0 0.0 0.0 3.287789460445456 1.8227270366012703E-4 88-89 0.0 0.0 0.0 3.656253730894403 1.8227270366012703E-4 90-91 0.0 0.0 0.0 4.0615826556586105 1.8227270366012703E-4 92-93 0.0 0.0 0.0 4.47037476179236 1.8227270366012703E-4 94-95 0.0 0.0 0.0 4.899125728976895 1.8227270366012703E-4 96-97 0.0 0.0 0.0 5.36647294116146 1.8227270366012703E-4 98-99 0.0 0.0 0.0 5.878431397566842 1.8227270366012703E-4 100-101 0.0 0.0 0.0 6.423244508806961 1.8227270366012703E-4 102-103 0.0 0.0 0.0 6.98118125471061 1.8227270366012703E-4 104-105 0.0 0.0 0.0 7.555203566712265 1.8227270366012703E-4 106-107 0.0 0.0 0.0 8.15920973846601 1.8227270366012703E-4 108-109 0.0 0.0 0.0 8.778390112799464 1.8227270366012703E-4 110-111 0.0 4.5568175915031756E-5 0.0 9.443958890214416 1.8227270366012703E-4 112-113 0.0 9.113635183006351E-5 0.0 10.144569594908031 1.8227270366012703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1820 0.0 69.95066 1 TACGGTG 35 9.157215E-5 67.99252 5 GTACATG 8785 0.0 48.147892 1 TACATGG 8725 0.0 47.319862 2 GTATCAA 2780 0.0 45.15305 2 ACATGGG 8705 0.0 44.355263 3 TCAACGC 3005 0.0 40.784203 5 ATCAACG 3075 0.0 39.85578 4 CAACGCA 3110 0.0 39.78984 6 CATGGGG 4015 0.0 39.563522 4 AACGCAG 3125 0.0 38.83733 7 CATGGGA 3345 0.0 35.92729 4 GGAACGC 100 1.0629441E-5 35.69608 8 TATCAAC 3715 0.0 33.630222 3 CATGGGC 1730 0.0 33.35761 4 CATGGGT 1755 0.0 32.204437 4 ATGGGGG 1750 0.0 30.596638 5 ACGCAGA 3895 0.0 30.39589 8 CGCAGAG 3815 0.0 30.253553 9 CTCGCTC 100 4.4413636E-4 29.74673 8 >>END_MODULE