FastQCFastQC Report
Thu 26 May 2016
SRR936275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097257
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.5270415226332573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.3494167729164635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36460.3322831387724116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11690.10653839528934424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11080.10097907782771037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.090.140661
CCACGCA807.2759576E-1266.973794
GTATCAA63450.057.2335362
CACGCAG1101.7644197E-1048.708215
TCAACGC88400.042.7635575
ATCAACG89450.042.2615854
GTACATG73800.042.2196541
CAACGCA89650.042.206766
AACGCAG91350.041.4213077
ATGGGCG1151.4191755E-841.4137465
TACATGG75700.040.8153762
TATCAAC93400.040.7929843
ACATGGG73450.040.4446563
CTATCGG650.001939776436.6118669
ACGCAGA103950.036.1716048
CGCAGAG104200.035.9135369
CATGGGG34600.035.0999454
CATGGGT15800.032.4036374
ACGCAGG750.003886135331.7737221
TATCCGT953.2880725E-431.3127786
Produced by FastQC (version 0.11.5)