##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1097257 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.580883056567423 31.0 31.0 34.0 30.0 34.0 2 31.46641671003238 31.0 31.0 34.0 30.0 34.0 3 31.8025795233022 31.0 31.0 34.0 30.0 34.0 4 35.6070118486371 37.0 35.0 37.0 33.0 37.0 5 35.25189631964071 37.0 35.0 37.0 33.0 37.0 6 35.46247050599814 37.0 35.0 37.0 33.0 37.0 7 35.29017267604581 37.0 35.0 37.0 32.0 37.0 8 35.3718235563774 37.0 35.0 37.0 33.0 37.0 9 37.053177149929326 39.0 37.0 39.0 33.0 39.0 10-11 36.943637634574216 39.0 37.0 39.0 33.0 39.0 12-13 36.9119518034517 39.0 37.0 39.0 33.0 39.0 14-15 38.011162836053906 40.0 37.0 41.0 33.0 41.0 16-17 37.94513728324358 40.0 37.0 41.0 32.5 41.0 18-19 38.12139362063765 40.0 37.0 41.0 33.0 41.0 20-21 38.203169357771245 40.0 37.5 41.0 33.0 41.0 22-23 38.10926246084554 40.0 37.0 41.0 33.0 41.0 24-25 38.078924080684835 40.0 37.0 41.0 33.0 41.0 26-27 37.89621483389944 40.0 37.0 41.0 33.0 41.0 28-29 37.74958008925894 40.0 37.0 41.0 32.0 41.0 30-31 37.52344482650828 39.5 36.5 41.0 32.0 41.0 32-33 37.253461130801625 39.0 36.0 41.0 32.0 41.0 34-35 37.01291766650839 39.0 35.0 41.0 31.0 41.0 36-37 36.769691603699044 39.0 35.0 41.0 31.0 41.0 38-39 36.71839049557214 39.0 35.0 41.0 30.5 41.0 40-41 36.6619684358359 40.0 35.0 41.0 30.5 41.0 42-43 36.509999480522794 40.0 35.0 41.0 30.0 41.0 44-45 36.244231752451796 40.0 35.0 41.0 30.0 41.0 46-47 35.778726861619475 40.0 35.0 41.0 28.0 41.0 48-49 35.36847657385644 40.0 35.0 41.0 24.5 41.0 50-51 35.16282329481608 40.0 35.0 41.0 24.0 41.0 52-53 35.06488634841245 40.0 35.0 41.0 23.5 41.0 54-55 34.95824177927322 39.0 35.0 41.0 23.0 41.0 56-57 34.82312302404997 39.0 35.0 41.0 23.0 41.0 58-59 34.67915128360995 39.0 35.0 41.0 22.0 41.0 60-61 34.54581196565618 39.0 34.5 41.0 22.0 41.0 62-63 34.41177727733795 39.0 34.0 41.0 21.5 41.0 64-65 34.25751669845806 39.0 34.0 40.5 20.5 41.0 66-67 34.055246856479386 38.0 34.0 40.0 20.0 41.0 68-69 33.83408763853865 38.0 34.0 40.0 19.0 41.0 70-71 33.57036318747568 37.5 34.0 40.0 18.5 41.0 72-73 33.336161901906294 37.0 34.0 40.0 18.5 41.0 74-75 33.17207910270794 37.0 34.0 39.0 19.0 41.0 76-77 32.863031176834596 36.0 33.0 39.0 18.0 41.0 78-79 32.504251966494635 36.0 33.0 39.0 17.5 40.0 80-81 32.1791963961041 35.5 33.0 37.5 16.5 39.0 82-83 31.85135934425572 35.0 32.5 37.0 14.5 39.0 84-85 31.50367416202403 35.0 32.0 37.0 12.5 39.0 86-87 31.229274910071204 35.0 32.0 36.0 12.0 37.5 88-89 30.987803677716343 35.0 32.0 36.0 10.5 37.0 90-91 30.76896387992968 35.0 32.0 36.0 9.0 37.0 92-93 30.578816084107917 35.0 32.0 35.0 7.0 36.0 94-95 30.413460110074485 35.0 32.0 35.0 7.0 36.0 96-97 30.301904202935138 35.0 32.0 35.0 7.0 36.0 98-99 30.1924230148452 35.0 32.0 35.0 2.0 36.0 100-101 30.103737775197608 35.0 32.0 35.0 2.0 36.0 102-103 29.157690039799245 34.0 29.5 35.0 2.0 35.0 104-105 29.847507922027383 34.0 31.0 35.0 2.0 35.0 106-107 29.970822696961605 35.0 32.0 35.0 2.0 35.0 108-109 29.936752283193456 35.0 32.0 35.0 2.0 35.0 110-111 29.915599535933694 35.0 32.0 35.0 2.0 35.0 112-113 29.845142022333874 35.0 32.0 35.0 2.0 35.0 114-115 29.823979705757175 35.0 32.0 35.0 2.0 35.0 116-117 29.728400912457154 35.0 32.0 35.0 2.0 35.0 118-119 29.609756875554226 35.0 31.5 35.0 2.0 35.0 120-121 29.437932954631414 34.0 31.0 35.0 2.0 35.0 122-123 29.233309516366724 34.0 31.0 35.0 2.0 35.0 124-125 27.328641330153282 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1485652617240305 1101 2 0.6089506358556562 1101 3 0.39159062546587364 1101 4 0.23744616083147463 1101 5 0.49803706725113983 1101 6 0.2530445245139319 1101 7 0.4040109126084843 1101 8 0.3417702327221832 1101 9 0.19170239231941366 1101 10-11 0.3453999135397794 1101 12-13 0.38356892498626394 1101 14-15 0.45052461230358176 1101 16-17 0.5555751480967714 1101 18-19 0.502755219867737 1101 20-21 0.4043422475300318 1101 22-23 0.2606777655812138 1101 24-25 0.22068134318056565 1101 26-27 0.33744493163884925 1101 28-29 0.37027258875657765 1101 30-31 0.2669928195564921 1101 32-33 0.1383727804571464 1101 34-35 0.066440943170619 1101 36-37 0.1847166915251819 1101 38-39 0.1746283878182595 1101 40-41 0.22799351960617287 1101 42-43 0.08894912674210076 1101 44-45 0.13241971745274128 1101 46-47 0.18497543948419093 1101 48-49 0.04720251566534017 1101 50-51 -0.02873730250043849 1101 52-53 -0.04332628261703064 1101 54-55 -0.11990274522747058 1101 56-57 -0.29407640333199936 1101 58-59 -0.07763461851907749 1101 60-61 0.01246303181587649 1101 62-63 -0.14554325616778385 1101 64-65 -0.23857088560051665 1101 66-67 -0.19091361544641927 1101 68-69 -0.24162187866556906 1101 70-71 -0.21172887083820768 1101 72-73 -0.15204484022360987 1101 74-75 -0.0796040710564867 1101 76-77 -0.1285622820958352 1101 78-79 -0.0024465414102579075 1101 80-81 -0.040125470693844534 1101 82-83 -0.10838430369627972 1101 84-85 -0.14069918758548638 1101 86-87 -0.06007692946088383 1101 88-89 -0.1206288218751368 1101 90-91 -0.09372629241105912 1101 92-93 -0.026261647573932123 1101 94-95 -0.035410150685596165 1101 96-97 -0.002211976954484385 1101 98-99 0.08596447266984342 1101 100-101 0.1311804823392606 1101 102-103 0.06851165840883766 1101 104-105 0.06786890115838418 1101 106-107 0.08851464414400212 1101 108-109 0.03220826754258965 1101 110-111 -0.0019735189740011094 1101 112-113 0.003779377946440121 1101 114-115 0.005915897131615111 1101 116-117 -0.014509202684347855 1101 118-119 5.429505676985968E-4 1101 120-121 0.017982401524619718 1101 122-123 -0.035764757283580195 1101 124-125 0.10042967450855045 1102 1 0.2437380253046797 1102 2 0.6320193805237793 1102 3 0.37152789494820126 1102 4 0.1412759905083547 1102 5 0.4721373851480166 1102 6 0.2708089752399161 1102 7 0.5221473748279877 1102 8 0.4275238690125107 1102 9 0.4262754810122189 1102 10-11 0.5020893295687472 1102 12-13 0.38752512821956486 1102 14-15 0.5981461155440968 1102 16-17 0.506565339556424 1102 18-19 0.57975395931539 1102 20-21 0.5320400205542128 1102 22-23 0.4464914584902857 1102 24-25 0.34176864385838 1102 26-27 0.47792171328317323 1102 28-29 0.3729390601861837 1102 30-31 0.38570119904640876 1102 32-33 0.355412201367038 1102 34-35 0.36906847329441916 1102 36-37 0.45791276471361897 1102 38-39 0.3885678897939755 1102 40-41 0.28654394806663674 1102 42-43 0.2570445255138054 1102 44-45 0.27481438493430943 1102 46-47 0.23460385241907034 1102 48-49 0.08448802016215495 1102 50-51 0.02313103132098604 1102 52-53 -0.006232831971679786 1102 54-55 0.06601334103335432 1102 56-57 0.10664289670067717 1102 58-59 0.12960368953953605 1102 60-61 0.09359888710277176 1102 62-63 0.05506017491140369 1102 64-65 0.008839040082065708 1102 66-67 -0.07869050192455518 1102 68-69 -0.010572076236989858 1102 70-71 -0.02632740401365652 1102 72-73 -0.057607929383287626 1102 74-75 -0.00642253454078201 1102 76-77 -9.610665329020662E-4 1102 78-79 0.02956915395201065 1102 80-81 0.04879280986182266 1102 82-83 0.04957998914233741 1102 84-85 0.07120305806518346 1102 86-87 0.07050642615493885 1102 88-89 -0.026649232636945186 1102 90-91 0.09816468466556572 1102 92-93 0.07862044621625586 1102 94-95 0.12130101729892218 1102 96-97 0.07743572625122397 1102 98-99 0.059466877056731704 1102 100-101 0.1298079869973634 1102 102-103 0.18197834714577965 1102 104-105 0.04421444529681651 1102 106-107 0.028491979667386147 1102 108-109 0.09228929092435223 1102 110-111 0.10831897585858385 1102 112-113 0.0794464732857918 1102 114-115 0.08216074563189579 1102 116-117 0.20101553936929406 1102 118-119 0.14266704494359672 1102 120-121 0.25858714833185914 1102 122-123 0.27583147548993026 1102 124-125 0.47100182521458933 1103 1 0.1769321476310992 1103 2 0.6789841884293963 1103 3 0.3683711383125683 1103 4 0.28167323205766337 1103 5 0.5094064405299079 1103 6 0.3294092463792637 1103 7 0.43634213578840786 1103 8 0.3885321824585546 1103 9 0.3302506975315467 1103 10-11 0.38197501968559777 1103 12-13 0.4136636053575842 1103 14-15 0.37501075874936873 1103 16-17 0.5325070445971463 1103 18-19 0.4537056752241 1103 20-21 0.44221740954954214 1103 22-23 0.3879556109557498 1103 24-25 0.3311940693342663 1103 26-27 0.21546835037099754 1103 28-29 0.289399151163515 1103 30-31 0.1888086576192336 1103 32-33 0.20431223834058443 1103 34-35 0.21597768082806112 1103 36-37 0.15723169516973456 1103 38-39 0.031030888236600163 1103 40-41 0.14723140395047096 1103 42-43 0.29179825928186176 1103 44-45 0.29205750281270326 1103 46-47 0.24148107223240345 1103 48-49 0.3415627123293632 1103 50-51 0.3335099541583162 1103 52-53 0.2771701068651069 1103 54-55 0.2806188725000851 1103 56-57 0.42736881067556 1103 58-59 0.41181479168736956 1103 60-61 0.3236030606319531 1103 62-63 0.3074627552168323 1103 64-65 0.3887282314818066 1103 66-67 0.22432802406336805 1103 68-69 0.32853487254904223 1103 70-71 0.26385191768981997 1103 72-73 0.25949835836054547 1103 74-75 0.2440529396729616 1103 76-77 0.2650797108079246 1103 78-79 0.2865514122514554 1103 80-81 0.31066005802154706 1103 82-83 0.18922585538772907 1103 84-85 0.24173864230325037 1103 86-87 0.18442486587125018 1103 88-89 0.2890481388799806 1103 90-91 0.34046046183832246 1103 92-93 0.32933890946118893 1103 94-95 0.21295760027092214 1103 96-97 0.1610267540037924 1103 98-99 0.2899766358323461 1103 100-101 0.3090190499308214 1103 102-103 0.38245760274503127 1103 104-105 0.38492778266363104 1103 106-107 0.28267251141421923 1103 108-109 0.4132077754570851 1103 110-111 0.4336350716760897 1103 112-113 0.42288590949521776 1103 114-115 0.41547657255087955 1103 116-117 0.42570896704118866 1103 118-119 0.39121541306567664 1103 120-121 0.34765558300632193 1103 122-123 0.3318513011540567 1103 124-125 0.3203472253692041 1104 1 0.1350665238560218 1104 2 0.47669346343974084 1104 3 0.22045189616783034 1104 4 0.1142878299815635 1104 5 0.3079274194262922 1104 6 0.1273823508502474 1104 7 0.3022300829622111 1104 8 0.22889240218395202 1104 9 0.26915053553340584 1104 10-11 0.2064501361131974 1104 12-13 0.14612768343465632 1104 14-15 0.28743788014381266 1104 16-17 0.29109178113773737 1104 18-19 0.2123409505250038 1104 20-21 0.21877527111925588 1104 22-23 0.339914664623457 1104 24-25 0.13282498082736538 1104 26-27 0.09580592873177096 1104 28-29 0.14738506335558554 1104 30-31 0.09263014169764006 1104 32-33 0.0728210057019325 1104 34-35 -0.018519939909893424 1104 36-37 0.09308293235859111 1104 38-39 -0.007567019218868154 1104 40-41 0.09856017931578975 1104 42-43 0.018022085343552874 1104 44-45 0.08945634991222562 1104 46-47 0.1348317817623439 1104 48-49 0.02088811067758911 1104 50-51 -0.10931922962870999 1104 52-53 -0.010023165689226232 1104 54-55 -0.10741104781656219 1104 56-57 0.02426035141219529 1104 58-59 0.054014245389069515 1104 60-61 -0.0390848407657316 1104 62-63 -0.26970205116660395 1104 64-65 -0.26854085263936156 1104 66-67 -0.2108225231943166 1104 68-69 -0.1511538566563928 1104 70-71 -0.20648063077942425 1104 72-73 -0.2654145093903111 1104 74-75 -0.1665868227845806 1104 76-77 -0.230912308522214 1104 78-79 -0.2027304302512647 1104 80-81 -0.13052772962109316 1104 82-83 -0.13285526566133754 1104 84-85 -0.20171054071627736 1104 86-87 -0.16978999821203544 1104 88-89 -0.2493494980303801 1104 90-91 -0.1915005928216651 1104 92-93 -0.2701898979748272 1104 94-95 -0.1898620171283909 1104 96-97 -0.06560378059007377 1104 98-99 -0.21160274325701067 1104 100-101 -0.2500801197944398 1104 102-103 -0.1772079437314389 1104 104-105 -0.22069188763862968 1104 106-107 -0.27805794670337036 1104 108-109 -0.4478762484013217 1104 110-111 -0.3255939912290735 1104 112-113 -0.28866420165579143 1104 114-115 -0.15447506785162446 1104 116-117 -0.22870579068798236 1104 118-119 -0.14577090754810484 1104 120-121 -0.17827782506727274 1104 122-123 0.014369035975981603 1104 124-125 -0.12937647323202128 1105 1 0.13351955298055884 1105 2 0.5283876857328735 1105 3 0.3100010342650634 1105 4 0.109732010725196 1105 5 0.3532167036447973 1105 6 0.27425317512579284 1105 7 0.4187398587278821 1105 8 0.3389121465049669 1105 9 0.36022836272738346 1105 10-11 0.244063732438228 1105 12-13 0.30478892539898084 1105 14-15 0.2285080622303255 1105 16-17 0.41347615719724473 1105 18-19 0.36063559296664494 1105 20-21 0.3130439334913646 1105 22-23 0.3374122215130271 1105 24-25 0.24877932291600757 1105 26-27 0.2536431610699168 1105 28-29 0.30781515050981056 1105 30-31 0.40222724453430203 1105 32-33 0.29976901232326014 1105 34-35 0.1889001354775175 1105 36-37 0.16457698579456093 1105 38-39 0.2568262251652982 1105 40-41 0.1054605535936588 1105 42-43 0.10849761102778643 1105 44-45 0.12609220347184902 1105 46-47 0.0375895049828614 1105 48-49 -0.03752632727405114 1105 50-51 0.057880766382538695 1105 52-53 -0.11980175672916005 1105 54-55 -0.222520937926447 1105 56-57 -0.19745142138850014 1105 58-59 -0.13991819845263365 1105 60-61 -0.1609784341656777 1105 62-63 -0.2013574496096595 1105 64-65 -0.25296294899430904 1105 66-67 -0.21342800905751602 1105 68-69 -0.15278492371396624 1105 70-71 -0.0696448410398176 1105 72-73 -0.14801308141720426 1105 74-75 -0.07057839987053427 1105 76-77 -0.00924956412806921 1105 78-79 -0.1080358024350403 1105 80-81 -0.12412844227807085 1105 82-83 0.03310489335689937 1105 84-85 0.08564627971676586 1105 86-87 0.027959348629870817 1105 88-89 0.12635883779592305 1105 90-91 -0.012147535111072472 1105 92-93 0.03462435915340478 1105 94-95 0.07728047437889174 1105 96-97 0.021562244552356447 1105 98-99 0.09670499558612278 1105 100-101 0.1354980497673992 1105 102-103 -0.004462918037233976 1105 104-105 0.09651940983582818 1105 106-107 0.08154896181461169 1105 108-109 0.10779226638697637 1105 110-111 0.15711044891638082 1105 112-113 0.12070554233282849 1105 114-115 0.02834155593595966 1105 116-117 0.011423544586541823 1105 118-119 0.05870397328793686 1105 120-121 0.08866173869624916 1105 122-123 0.12454550500000039 1105 124-125 -0.0010329021026045382 1106 1 0.23650177485367507 1106 2 0.4831437928729265 1106 3 0.24866780566296853 1106 4 0.1265557742420711 1106 5 0.2628192486137664 1106 6 0.26643760682793527 1106 7 0.20462339759626502 1106 8 0.2225622918195569 1106 9 0.01877966794038599 1106 10-11 0.18178595902425343 1106 12-13 0.12417205843382817 1106 14-15 0.10152864191770306 1106 16-17 0.20438221328082307 1106 18-19 0.20777904857369833 1106 20-21 0.21195407409304323 1106 22-23 0.010236430137645414 1106 24-25 0.038293762734816994 1106 26-27 0.11222719479336973 1106 28-29 0.11795378848012206 1106 30-31 0.10846529862799059 1106 32-33 0.10422074431603079 1106 34-35 0.15077469364263152 1106 36-37 0.3211213787461489 1106 38-39 0.37766774062625075 1106 40-41 0.2210844899170752 1106 42-43 0.31847457015642533 1106 44-45 0.25676078148352843 1106 46-47 0.25923941465217126 1106 48-49 0.42670141079312884 1106 50-51 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2213 86-87 0.1778907749621581 2213 88-89 0.0751963508575777 2213 90-91 0.04139217230794756 2213 92-93 0.13849713867273294 2213 94-95 0.2498934576599794 2213 96-97 0.16679474194003063 2213 98-99 0.10167821920158815 2213 100-101 0.050750792714104875 2213 102-103 0.12774384789798177 2213 104-105 0.2063678946843126 2213 106-107 0.11345530977699525 2213 108-109 0.16724563629293243 2213 110-111 0.21555154087040407 2213 112-113 0.17868621108936011 2213 114-115 0.05583633127556453 2213 116-117 -0.12017911999026154 2213 118-119 0.029403424698593028 2213 120-121 -0.019201375201348014 2213 122-123 -0.15203495477011586 2213 124-125 -0.2953706763972761 2214 1 -0.1720362271598539 2214 2 0.04528545021522845 2214 3 -0.18143217644493603 2214 4 -0.2101114087139706 2214 5 0.11446705736594254 2214 6 -0.09918539867054932 2214 7 -0.30907340345696355 2214 8 -0.25778510861893267 2214 9 -0.1380185278952979 2214 10-11 -0.11462500642797835 2214 12-13 -0.17368596376839207 2214 14-15 -0.1566700111011201 2214 16-17 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72-73 -0.32889688736522515 2215 74-75 -0.2202531685034117 2215 76-77 -0.26766194103417007 2215 78-79 -0.281485395689991 2215 80-81 -0.2948592659779621 2215 82-83 -0.12796697042467642 2215 84-85 -0.15591664226063884 2215 86-87 -0.2141066527533937 2215 88-89 -0.23790276349511785 2215 90-91 -0.27851861175040327 2215 92-93 -0.2548719027442985 2215 94-95 -0.1453844467422094 2215 96-97 -0.1791384437854191 2215 98-99 -0.20103814573720058 2215 100-101 -0.1096531210655236 2215 102-103 -0.10522453579568491 2215 104-105 -0.17155604384134904 2215 106-107 -0.3249474318223804 2215 108-109 -0.23480974922413012 2215 110-111 -0.22278119551927844 2215 112-113 -0.18580649534460747 2215 114-115 -0.2380064663279775 2215 116-117 -0.28673128201162257 2215 118-119 -0.3065637868148201 2215 120-121 -0.43953870263036876 2215 122-123 -0.4670421180938966 2215 124-125 -0.529476237741374 2216 1 -0.23046600011445406 2216 2 0.10186127004163836 2216 3 -0.13232997912732003 2216 4 -0.12872232861889188 2216 5 -0.44577095848744364 2216 6 -0.3581614088666143 2216 7 -0.11723899817393857 2216 8 -0.1971128455861404 2216 9 -0.2639952479443437 2216 10-11 -0.914889789653941 2216 12-13 -0.43735029742079945 2216 14-15 -0.4494557941442423 2216 16-17 -0.48985941698813207 2216 18-19 -0.8365284110405184 2216 20-21 -0.5880474932723203 2216 22-23 -0.6274703314765375 2216 24-25 -0.6103251271525352 2216 26-27 -1.0990412478723997 2216 28-29 -1.6624968551845853 2216 30-31 -1.263031639784998 2216 32-33 -0.8858506491762697 2216 34-35 -1.335390041681606 2216 36-37 -1.0580589152915891 2216 38-39 -0.7560468107937908 2216 40-41 -0.5646174807836459 2216 42-43 -0.3161412163475177 2216 44-45 -0.8590061303887424 2216 46-47 -0.5620783216542833 2216 48-49 -0.5343462945839761 2216 50-51 -0.4289647730037416 2216 52-53 -0.46781201192266053 2216 54-55 -0.6629452498530597 2216 56-57 -1.1579840851025196 2216 58-59 -1.218068572806743 2216 60-61 -0.7826692229455929 2216 62-63 -0.8200672460875751 2216 64-65 -0.7009020241814881 2216 66-67 -0.724584156901031 2216 68-69 -0.543750335503475 2216 70-71 -0.43784978673211583 2216 72-73 -0.522390256361831 2216 74-75 -0.5637240288908814 2216 76-77 -0.3277952511469451 2216 78-79 -0.4527810043753533 2216 80-81 -0.36314233556078435 2216 82-83 -0.42369345632147315 2216 84-85 -0.29790982067340366 2216 86-87 -0.2232586203672824 2216 88-89 -0.238325333879704 2216 90-91 -0.2074193372833797 2216 92-93 -0.3254884743381048 2216 94-95 -0.4878228684451109 2216 96-97 -0.3556614715712314 2216 98-99 -0.48313985836305307 2216 100-101 -0.5852766259681275 2216 102-103 -0.6060473322521389 2216 104-105 -0.3924905290020959 2216 106-107 -0.36441948171591676 2216 108-109 -0.353659805742101 2216 110-111 -0.4669312572947355 2216 112-113 -0.3648969832392446 2216 114-115 -0.3529209664725599 2216 116-117 -0.309691213401436 2216 118-119 -0.46005393560165686 2216 120-121 -0.4518587980537241 2216 122-123 -0.5629294501829563 2216 124-125 -0.8607272235198771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66.0 3 55.0 4 137.0 5 305.0 6 704.0 7 1357.0 8 2144.0 9 4084.0 10 7292.0 11 9507.0 12 10562.0 13 13930.0 14 20076.0 15 14555.0 16 5121.0 17 3333.0 18 2889.0 19 2960.0 20 2985.0 21 3054.0 22 3256.0 23 3985.0 24 5080.0 25 6697.0 26 8822.0 27 11002.0 28 14127.0 29 18286.0 30 23250.0 31 29541.0 32 37676.0 33 48306.0 34 64428.0 35 88825.0 36 138963.0 37 276110.0 38 211718.0 39 2069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.01650869620333 20.41252588888595 12.544584514729875 21.026380900180847 2 16.932679942875424 23.310163308121844 40.47250811345809 19.284648635544645 3 16.39817872948519 30.219930444672894 34.45695443907347 18.924936386768447 4 10.054253470244436 19.2994895452934 40.46991725730618 30.176339727155987 5 8.653562035115112 42.36302881511124 38.243952620500714 10.739456529272928 6 25.782565069076796 41.31538919323367 19.90663992118528 12.995405816504245 7 21.87226875745609 36.54494799304083 25.854107105263395 15.728676144239682 8 23.98180189326657 36.304256887857626 22.732049100620912 16.981892118254883 9 25.423943524625496 12.208625691155309 22.791014320254963 39.57641646396423 10-11 21.755568658937698 27.287043965087488 32.58785316475539 18.36953421121943 12-13 23.43534832769351 27.492146324881045 32.684366561343424 16.38813878608202 14-15 20.457650304349848 29.16080735871359 30.791464533833 19.590077803103554 16-17 17.675174400880742 30.534792913051778 32.26196427179971 19.528068414267764 18-19 17.060952903467463 28.45144756424429 36.05659385175943 18.43100568052881 20-21 18.840526877477203 27.587885062478524 36.38614289997694 17.18544516006733 22-23 18.393867617158058 27.87979479739022 36.185688494126715 17.540649091325005 24-25 17.92847983653784 28.01859546122741 36.73956967237393 17.313355029860826 26-27 17.364528091413405 28.120212493517926 36.783770803011514 17.731488612057156 28-29 17.392689224128898 27.873324116410288 36.530776290331254 18.203210369129565 30-31 17.741240201702972 28.274597473518053 36.36386006195449 17.62030226282448 32-33 17.64167373732863 28.22105486681789 36.365227107231945 17.772044288621537 34-35 17.75527008547242 28.731802877090047 36.25608279574155 17.25684424169598 36-37 17.702142706767876 28.616860042815855 35.7612209354782 17.91977631493807 38-39 18.055159365581627 28.55001152874851 35.590476980324574 17.804352125345293 40-41 18.476664992795673 28.243474409368087 35.61982288561386 17.660037712222387 42-43 18.16710769809845 28.850017566827997 35.12426878932885 17.858605945744703 44-45 18.049369145696676 29.377722362128505 34.47755711776645 18.09535137440836 46-47 18.666607726024452 29.121887729014016 33.389038316582344 18.822466228379188 48-49 19.18130467178133 29.47052537317514 32.460665939038755 18.887504016004776 50-51 19.12219548196491 29.96681038352395 31.94665781974798 18.96433631476316 52-53 18.777906286826727 30.22140924299056 32.206646196360005 18.794038273822707 54-55 18.950633683787437 29.860539894667816 32.44941307178165 18.7394133497631 56-57 18.91258341642317 30.167397917984406 31.893874076517992 19.02614458907443 58-59 18.78983881286945 29.899866391307594 32.22165919029123 19.088635605531728 60-61 18.842954568860314 29.836508412448293 32.12346763661286 19.19706938207854 62-63 18.712397419545347 29.63357890213566 32.71351020302147 18.94051347529753 64-65 18.74476529854317 29.881020191479568 32.55936459040597 18.814849919571287 66-67 18.740627020701552 30.28367175171442 31.97437380181939 19.001327425764636 68-69 18.799279471077455 30.08759630330203 32.29835146883184 18.814772756788678 70-71 19.16766310985057 29.403975079199007 32.56268478248982 18.865677028460603 72-73 18.77573507297414 29.391481155410222 32.693043806357416 19.139739965258222 74-75 18.646089798008934 29.872094045895587 32.61576767040191 18.86604848569357 76-77 18.49388290019066 29.665655656130042 32.64619248814311 19.194268955536195 78-79 18.371555954125565 29.5380020706198 32.60014071465547 19.490301260599168 80-81 18.575245886101328 29.88673563872059 32.14085865103495 19.397159824143134 82-83 18.828186481465366 29.59581465227137 32.32762804321506 19.248370823048212 84-85 18.520845874092224 29.536785188135305 32.58065236401185 19.361716573760624 86-87 18.18121336591772 29.99452724461576 32.31194979100089 19.512309598465624 88-89 18.354137645209814 29.85135090182397 32.12991405319761 19.664597399768603 90-91 18.572787829794294 29.596614040540008 32.18229060813662 19.648307521529077 92-93 18.456164206282228 29.763937976074818 32.18024267854116 19.59965513910179 94-95 18.37785840380516 29.668479385225023 32.26456759508115 19.689094615888674 96-97 18.47105458983009 29.662386710734552 31.81737193966898 20.04918675976638 98-99 18.578948111915707 29.577032845699176 31.82768710300833 20.016331939376787 100-101 18.867834251233308 29.34729610636035 31.756134193543684 20.02873544886266 102-103 18.43317384370016 29.6937343358396 31.705718842560948 20.167372977899294 104-105 18.20696776404348 29.590714265534018 31.598500983820504 20.603816986602 106-107 18.48348488611327 29.16306866468748 31.399700615856425 20.953745833342825 108-109 18.55044171732734 29.463163185468417 31.169654359096345 20.8167407381079 110-111 18.535093611215668 29.498264298688266 31.26062313909944 20.706018950996626 112-113 18.27019612221693 29.600896871204647 31.24516074639007 20.883746260188353 114-115 18.263971527576167 29.608504172282046 30.811581666114435 21.315942634027355 116-117 18.421870599076996 29.56955383926838 30.573226345423404 21.43534921623122 118-119 18.37255150375563 29.286845134634827 30.742521781374155 21.59808158023539 120-121 18.496045643144072 29.044551737023305 31.018016191103325 21.4413864287293 122-123 18.4158828228494 29.09341461825238 31.086651102956086 21.404051455942135 124-125 18.209309309446155 29.344425674674984 30.620111764939097 21.826153250939768 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 94.5 2 156.0 3 244.0 4 193.5 5 177.5 6 270.5 7 712.5 8 1168.5 9 1378.5 10 1304.5 11 1070.5 12 1114.5 13 1263.5 14 1521.5 15 1868.5 16 2226.5 17 2593.5 18 3068.0 19 3723.0 20 4471.5 21 5339.5 22 6481.0 23 7957.0 24 9647.5 25 11603.0 26 14330.5 27 17357.0 28 20965.0 29 25330.0 30 30500.0 31 36207.5 32 42524.0 33 49708.0 34 55713.5 35 58872.5 36 59700.5 37 58410.5 38 55110.0 39 51418.5 40 47773.5 41 43873.0 42 39476.0 43 35835.5 44 32920.0 45 30434.5 46 27812.5 47 25103.0 48 23497.5 49 21596.0 50 19567.0 51 17472.5 52 15451.0 53 13804.0 54 11593.5 55 9398.0 56 7771.5 57 6168.0 58 4833.5 59 3797.0 60 2879.5 61 2303.5 62 1729.0 63 1252.5 64 996.0 65 788.0 66 574.0 67 548.5 68 505.5 69 375.5 70 301.0 71 229.5 72 176.0 73 142.5 74 126.5 75 109.0 76 73.0 77 43.0 78 26.0 79 10.0 80 6.5 81 5.5 82 5.0 83 5.5 84 3.0 85 3.0 86 3.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06780544576156726 2 5.46818110980381E-4 3 9.113635183006351E-5 4 0.0 5 0.06826112752071757 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.5568175915031756E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.278408795751588E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.001686022508856175 44-45 0.00856681707202597 46-47 0.00984272599764686 48-49 0.007701021729640367 50-51 0.006971930914999858 52-53 0.0052859084061436835 54-55 0.005604885637548906 56-57 0.0046479539433332395 58-59 0.002096136092091461 60-61 0.0012759089256208893 62-63 7.746589905555398E-4 64-65 0.001093636221960762 66-67 0.001868295212516302 68-69 6.835226387254764E-4 70-71 0.0019138633884313338 72-73 0.0017315906847712068 74-75 5.012499350653493E-4 76-77 4.556817591503176E-4 78-79 9.113635183006351E-5 80-81 9.113635183006351E-5 82-83 4.5568175915031756E-5 84-85 1.3670452774509526E-4 86-87 3.189772314052223E-4 88-89 7.746589905555398E-4 90-91 0.0018227270366012703 92-93 2.734090554901905E-4 94-95 9.113635183006352E-4 96-97 9.569316942156669E-4 98-99 0.001868295212516302 100-101 5.46818110980381E-4 102-103 6.379544628104446E-4 104-105 3.6454540732025405E-4 106-107 4.101135832352858E-4 108-109 5.012499350653493E-4 110-111 5.46818110980381E-4 112-113 0.009979430525391955 114-115 0.017817156782777416 116-117 0.016951361440391815 118-119 0.009478180590326605 120-121 0.004511249415588144 122-123 0.004237840360097954 124-125 0.003964431304607763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1097257.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.634922331996705 #Duplication Level Percentage of deduplicated Percentage of total 1 42.27782485760392 13.374557057360596 2 17.066615743015674 10.798021270006663 3 12.276102859171193 11.6506068146845 4 8.871970985930618 11.226564522865734 5 6.232084110598689 9.857574840263014 6 4.122297535409102 7.8245137417229005 7 2.751499057761533 6.093042129219374 8 1.7653838717821728 4.467822533599093 9 1.1899736991839052 3.388025299572148 >10 3.3229423889557643 15.274377062136304 >50 0.07921330187803274 1.7026485096469288 >100 0.03916029337205802 2.2130141205253504 >500 0.00348091435577479 0.7031981024013829 >1k 0.0011603047852582636 0.8953548068811552 >5k 2.900761963145659E-4 0.5306791891148632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5783 0.5270415226332573 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3834 0.3494167729164635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3646 0.3322831387724116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1169 0.10653839528934424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1108 0.10097907782771037 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8227270366012703E-4 0.0 10-11 0.0 0.0 0.0 1.8227270366012703E-4 0.0 12-13 0.0 0.0 0.0 1.8227270366012703E-4 0.0 14-15 0.0 0.0 0.0 3.6454540732025405E-4 0.0 16-17 0.0 0.0 0.0 3.6454540732025405E-4 0.0 18-19 0.0 0.0 0.0 4.556817591503176E-4 0.0 20-21 0.0 0.0 0.0 4.556817591503176E-4 0.0 22-23 0.0 0.0 0.0 4.556817591503176E-4 0.0 24-25 0.0 0.0 0.0 6.379544628104445E-4 0.0 26-27 0.0 0.0 0.0 9.569316942156669E-4 0.0 28-29 0.0 0.0 0.0 0.0012303407497058574 0.0 30-31 0.0 0.0 0.0 0.0030530677863071275 0.0 32-33 0.0 0.0 0.0 0.00751874902598024 0.0 34-35 0.0 0.0 0.0 0.013351475543104304 0.0 36-37 0.0 0.0 0.0 0.022601815253855753 0.0 38-39 0.0 0.0 0.0 0.039826585749737756 0.0 40-41 0.0 0.0 0.0 0.06056010579107721 0.0 42-43 0.0 0.0 0.0 0.08626055700715513 0.0 44-45 0.0 0.0 0.0 0.11847725737908257 0.0 46-47 0.0 0.0 0.0 0.15561532074983345 0.0 48-49 0.0 0.0 0.0 0.19922406510051882 0.0 50-51 0.0 0.0 0.0 0.24342519573809965 0.0 52-53 0.0 0.0 0.0 0.28976803064368695 0.0 54-55 0.0 0.0 0.0 0.34668268236156163 0.0 56-57 0.0 0.0 0.0 0.4185436957795667 0.0 58-59 0.0 0.0 0.0 0.505168798194042 0.0 60-61 0.0 0.0 0.0 0.5939356048765239 0.0 62-63 0.0 0.0 0.0 0.6951425235838093 0.0 64-65 0.0 0.0 0.0 0.8058276228814216 0.0 66-67 0.0 0.0 0.0 0.9412562417008961 0.0 68-69 0.0 0.0 0.0 1.0976917896172 0.0 70-71 0.0 0.0 0.0 1.2729014260104972 0.0 72-73 0.0 0.0 0.0 1.446425039894938 0.0 74-75 0.0 0.0 0.0 1.6369000152197708 0.0 76-77 0.0 0.0 0.0 1.8544424870381324 0.0 78-79 0.0 0.0 0.0 2.1186012028175716 0.0 80-81 0.0 0.0 0.0 2.3917824174281868 0.0 82-83 0.0 0.0 0.0 2.6739405626940638 0.0 84-85 0.0 0.0 0.0 2.9770600688808546 0.0 86-87 0.0 0.0 0.0 3.3082040032553905 0.0 88-89 0.0 0.0 0.0 3.677442932694893 0.0 90-91 0.0 0.0 0.0 4.0850046980789365 0.0 92-93 0.0 0.0 0.0 4.497351121934059 0.0 94-95 0.0 0.0 0.0 4.932208224691207 0.0 96-97 0.0 0.0 0.0 5.405524867920642 0.0 98-99 0.0 0.0 0.0 5.923543891722723 0.0 100-101 0.0 0.0 0.0 6.4745542748872875 0.0 102-103 0.0 0.0 0.0 7.038004770076655 0.0 104-105 0.0 0.0 0.0 7.616401626966153 0.0 106-107 0.0 0.0 0.0 8.228610070384605 0.0 108-109 0.0 0.0 0.0 8.85407885299433 0.0 110-111 0.0 0.0 0.0 9.52794103842582 0.0 112-113 0.0 0.0 0.0 10.239624809866786 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4025 0.0 90.14066 1 CCACGCA 80 7.2759576E-12 66.97379 4 GTATCAA 6345 0.0 57.233536 2 CACGCAG 110 1.7644197E-10 48.70821 5 TCAACGC 8840 0.0 42.763557 5 ATCAACG 8945 0.0 42.261585 4 GTACATG 7380 0.0 42.219654 1 CAACGCA 8965 0.0 42.20676 6 AACGCAG 9135 0.0 41.421307 7 ATGGGCG 115 1.4191755E-8 41.413746 5 TACATGG 7570 0.0 40.815376 2 TATCAAC 9340 0.0 40.792984 3 ACATGGG 7345 0.0 40.444656 3 CTATCGG 65 0.0019397764 36.611866 9 ACGCAGA 10395 0.0 36.171604 8 CGCAGAG 10420 0.0 35.913536 9 CATGGGG 3460 0.0 35.099945 4 CATGGGT 1580 0.0 32.403637 4 ACGCAGG 75 0.0038861353 31.773722 1 TATCCGT 95 3.2880725E-4 31.312778 6 >>END_MODULE