SRR936274_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR936274_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66398
Sequences flagged as poor quality	0
Sequence length	125
%GC	30

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2808	4.229043043465165	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1079	1.6250489472574476	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	776	1.1687099009006294	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	702	1.0572607608662912	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	571	0.8599656616163137	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	324	0.4879665050152113	No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	321	0.4834482966354408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	305	0.45935118527666496	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.3388656284827856	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	203	0.30573210036446885	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.22892255790837074	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.22892255790837074	No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.22741648844844725	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	128	0.19277689087020694	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	112	0.16867977951143107	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	107	0.16114943221181363	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	105	0.15813729329196663	No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.15512515437211963	No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG	89	0.13404018193319076	No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87	0.13102804301334378	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	87	0.13102804301334378	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	84	0.1265098346335733	No Hit
GAACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.1250037651736498	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	78	0.11747341787403236	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	78	0.11747341787403236	No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG	76	0.11446127895418537	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	72	0.10843700111449141	No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71	0.1069309316545679	No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG	69	0.10391879273472092	No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG	69	0.10391879273472092	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	69	0.10391879273472092	No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT	68	0.10241272327479745	No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG	67	0.10090665381487395	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTGTG	20	8.44912E-4	89.242096	7
CTGTGCG	20	8.44912E-4	89.242096	9
GAACAAA	335	0.0	88.798096	1
GGAATCA	25	0.00204901	71.39367	1
CCTGTGC	25	0.00204901	71.39367	8
GGAAACA	90	0.0	66.10525	1
GAAAACA	245	0.0	63.137257	1
CTAGATA	30	0.0042204848	59.494728	4
GCGAATG	20	1.7670209E-4	59.494728	80-81
ACGTTAA	20	1.7670209E-4	59.494728	104-105
GGATCTG	15	0.004229794	59.494728	30-31
GCTGCGT	20	1.7670209E-4	59.494728	92-93
CGCGAAT	20	1.7670209E-4	59.494728	78-79
TAACGCG	20	1.7670209E-4	59.494728	76-77
CTGCGTA	20	1.7670209E-4	59.494728	92-93
TCTACGT	20	1.7670209E-4	59.494728	100-101
CTACGTT	20	1.7670209E-4	59.494728	102-103
ATATAGG	40	1.7605486E-4	59.494728	2
AACGCGA	20	1.7670209E-4	59.494728	76-77
AGGGGTC	40	1.7605486E-4	59.494728	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content