##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66398 Sequences flagged as poor quality 0 Sequence length 125 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.88541823548902 30.0 12.0 33.0 7.0 34.0 2 24.42481701256062 30.0 16.0 33.0 7.0 34.0 3 25.128196632428686 30.0 22.0 33.0 7.0 34.0 4 26.932979909033406 35.0 17.0 37.0 7.0 37.0 5 29.30074701045212 35.0 28.0 37.0 7.0 37.0 6 27.606192957619207 35.0 19.0 37.0 7.0 37.0 7 28.259766860447606 35.0 25.0 37.0 7.0 37.0 8 27.318036687852043 35.0 18.0 37.0 7.0 37.0 9 27.51810295490828 35.0 15.0 39.0 2.0 39.0 10-11 28.364137474020303 35.0 20.5 39.0 2.0 39.0 12-13 28.533510045483297 35.0 21.0 39.0 2.0 39.0 14-15 29.286514654055843 36.0 20.5 40.0 2.0 41.0 16-17 29.403882647067682 36.0 23.0 40.0 2.0 41.0 18-19 29.15085544745324 36.0 22.5 40.0 2.0 41.0 20-21 28.965285098948762 35.0 22.5 40.0 2.0 41.0 22-23 28.6378354769722 35.0 22.5 40.0 2.0 41.0 24-25 28.390049399078286 35.0 23.0 40.0 2.0 41.0 26-27 28.24346365854393 35.0 23.0 40.0 2.0 41.0 28-29 27.94072863640471 35.0 21.0 40.0 2.0 41.0 30-31 27.718613512455192 35.0 18.5 40.0 2.0 41.0 32-33 27.45887677339679 35.0 16.0 40.0 2.0 41.0 34-35 27.263087743606732 35.0 9.5 40.0 2.0 41.0 36-37 26.98186692370252 35.0 2.0 40.0 2.0 41.0 38-39 26.643603723003707 34.0 2.0 40.0 2.0 41.0 40-41 26.636321877164974 34.5 2.0 40.0 2.0 41.0 42-43 26.35578631886503 34.0 2.0 40.0 2.0 41.0 44-45 26.307200518087896 34.0 2.0 40.0 2.0 41.0 46-47 26.225579083707338 34.0 2.0 40.0 2.0 41.0 48-49 26.083263050091873 33.0 2.0 40.0 2.0 41.0 50-51 26.00476670984066 33.0 2.0 40.0 2.0 41.0 52-53 25.884687791800957 33.0 2.0 40.0 2.0 41.0 54-55 25.76220669297268 33.0 2.0 40.0 2.0 41.0 56-57 25.598911111780474 33.0 2.0 40.0 2.0 41.0 58-59 25.385561312087713 33.0 2.0 40.0 2.0 41.0 60-61 25.17153378113799 33.0 2.0 39.0 2.0 41.0 62-63 24.918596945691135 33.0 2.0 39.0 2.0 41.0 64-65 24.638016205307387 32.5 2.0 39.0 2.0 41.0 66-67 24.336531220819907 32.0 2.0 38.5 2.0 40.5 68-69 24.076387843007318 32.0 2.0 38.0 2.0 40.0 70-71 23.783088346034518 32.0 2.0 37.0 2.0 40.0 72-73 23.465405584505557 31.0 2.0 37.0 2.0 40.0 74-75 23.144989306906837 31.0 2.0 36.5 2.0 39.0 76-77 22.845966745986324 31.0 2.0 36.0 2.0 39.0 78-79 22.54291544926052 31.0 2.0 35.5 2.0 38.5 80-81 22.27817102924787 31.0 2.0 35.0 2.0 37.5 82-83 21.98958552968463 30.0 2.0 35.0 2.0 37.0 84-85 21.859001777161964 30.0 2.0 35.0 2.0 37.0 86-87 21.68441067502033 30.0 2.0 35.0 2.0 36.0 88-89 21.472453989578 30.0 2.0 35.0 2.0 36.0 90-91 21.264262477785476 30.0 2.0 35.0 2.0 36.0 92-93 21.12563631434682 30.0 2.0 35.0 2.0 35.0 94-95 20.997356848097837 30.0 2.0 35.0 2.0 35.0 96-97 20.890817494502848 30.0 2.0 35.0 2.0 35.0 98-99 20.78465465827284 29.5 2.0 35.0 2.0 35.0 100-101 20.684606464050123 29.0 2.0 35.0 2.0 35.0 102-103 20.005971565408597 28.0 2.0 34.0 2.0 35.0 104-105 20.418634597427634 29.0 2.0 35.0 2.0 35.0 106-107 20.453040754239584 29.0 2.0 35.0 2.0 35.0 108-109 20.403837464983887 29.0 2.0 35.0 2.0 35.0 110-111 20.309723184433267 29.0 2.0 35.0 2.0 35.0 112-113 20.252703394680562 29.0 2.0 35.0 2.0 35.0 114-115 20.11002590439471 29.0 2.0 35.0 2.0 35.0 116-117 20.00545197144492 29.0 2.0 35.0 2.0 35.0 118-119 19.864054640200006 28.0 2.0 35.0 2.0 35.0 120-121 19.712303081418113 28.0 2.0 35.0 2.0 35.0 122-123 19.494788999668664 28.0 2.0 35.0 2.0 35.0 124-125 18.261280460254827 24.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4369573952543533 1101 2 1.473991443924465 1101 3 1.5959557481538802 1101 4 1.4322400198367298 1101 5 1.1728504765943626 1101 6 1.4213100948167678 1101 7 1.2527893716524545 1101 8 1.6436261210991994 1101 9 1.620663785807249 1101 10-11 2.1210201049968838 1101 12-13 1.028391194345371 1101 14-15 1.5434986815683551 1101 16-17 1.5290347046125383 1101 18-19 1.9644775507905443 1101 20-21 1.6983952464856422 1101 22-23 2.835409464105311 1101 24-25 1.8694999012627989 1101 26-27 1.6463379517675065 1101 28-29 1.1206941068863792 1101 30-31 0.7622394377835029 1101 32-33 1.2277495801456837 1101 34-35 1.1341362586326262 1101 36-37 1.3422248154721927 1101 38-39 1.5972476580346218 1101 40-41 1.553554759944852 1101 42-43 1.358336618086085 1101 44-45 1.3664750468546991 1101 46-47 1.0882142093138327 1101 48-49 1.2501055750146755 1101 50-51 1.436615652315659 1101 52-53 1.3192828878438405 1101 54-55 0.9370166796294903 1101 56-57 0.720672366705255 1101 58-59 1.2176721334813578 1101 60-61 1.308048211910286 1101 62-63 1.2518954103419446 1101 64-65 0.9314552083373826 1101 66-67 1.2881585728983183 1101 68-69 0.6832796892744888 1101 70-71 0.7758186891353915 1101 72-73 0.8992672114712228 1101 74-75 1.0790529391522306 1101 76-77 1.1809392768392541 1101 78-79 0.8570263970240752 1101 80-81 1.3918529900977852 1101 82-83 1.8502450933411012 1101 84-85 1.655679712144451 1101 86-87 1.7690691983633187 1101 88-89 1.8282854827509816 1101 90-91 1.7642693825028992 1101 92-93 1.6315721720783678 1101 94-95 2.000049468866454 1101 96-97 1.998113988854854 1101 98-99 2.281023038620752 1101 100-101 1.800425282898324 1101 102-103 2.064576055872603 1101 104-105 2.2554209308421704 1101 106-107 2.095571170643254 1101 108-109 2.067931034714441 1101 110-111 1.837055728927524 1101 112-113 1.9962929888776202 1101 114-115 1.8875938203685756 1101 116-117 2.1212022468745246 1101 118-119 1.9383035745370876 1101 120-121 1.9908025680575037 1101 122-123 2.000674607287806 1101 124-125 1.914763474736251 1102 1 0.6368533730629515 1102 2 0.8141440098051866 1102 3 0.05304659420103519 1102 4 1.0028017396702928 1102 5 1.4296986041949147 1102 6 1.3965181391995714 1102 7 0.37952307484246717 1102 8 0.7826691169383118 1102 9 0.9339439522427533 1102 10-11 1.4457860550662325 1102 12-13 1.6726352997545249 1102 14-15 1.4694695553547632 1102 16-17 1.2517722219495724 1102 18-19 1.546785109736451 1102 20-21 0.9643453158337714 1102 22-23 1.646652529292549 1102 24-25 1.3864035073654328 1102 26-27 1.3034981459422639 1102 28-29 1.3560443149307595 1102 30-31 0.667449215869496 1102 32-33 0.6008425066366669 1102 34-35 0.5878897260390055 1102 36-37 0.2469144826011842 1102 38-39 0.294456395898699 1102 40-41 0.6252000442721766 1102 42-43 1.0363445931207593 1102 44-45 0.7943096515703729 1102 46-47 0.34090144925835375 1102 48-49 0.5280181963738997 1102 50-51 0.5951194664626769 1102 52-53 0.5943348989395432 1102 54-55 0.25536792789578655 1102 56-57 0.38663110456933225 1102 58-59 0.6690417590014661 1102 60-61 0.718112358928316 1102 62-63 0.7162244672074074 1102 64-65 0.6056490363540235 1102 66-67 0.3383059376694675 1102 68-69 0.5691586767918224 1102 70-71 0.7650263867775529 1102 72-73 0.6911361435100574 1102 74-75 0.6976382373491781 1102 76-77 0.8252267248281626 1102 78-79 0.7946564247633212 1102 80-81 0.7187427265748987 1102 82-83 0.3575699893189075 1102 84-85 0.5942199340584615 1102 86-87 0.5457682274895319 1102 88-89 0.5279647476608247 1102 90-91 0.637579021892634 1102 92-93 0.4512236977371771 1102 94-95 0.5439988447333057 1102 96-97 0.787035625470665 1102 98-99 0.6395528583156178 1102 100-101 0.6141388751313315 1102 102-103 0.9205920059419554 1102 104-105 1.2936490861819756 1102 106-107 1.230323251087082 1102 108-109 1.2803530180708904 1102 110-111 1.273212108955267 1102 112-113 0.9937357766723522 1102 114-115 1.0974585915197537 1102 116-117 1.356639140078407 1102 118-119 1.1672824233581665 1102 120-121 0.6602287123016097 1102 122-123 0.49469333128225657 1102 124-125 -0.25435927144960147 1103 1 1.7831376443472244 1103 2 2.040334485995661 1103 3 1.5771266807811237 1103 4 2.0982202100887655 1103 5 2.298475660471972 1103 6 1.2137980814795135 1103 7 2.55719262751202 1103 8 2.2729288571980497 1103 9 2.297039190337994 1103 10-11 2.0615418270720056 1103 12-13 1.5406006677198896 1103 14-15 2.243459454344663 1103 16-17 2.7251217890491404 1103 18-19 1.998850405551746 1103 20-21 2.2756728771613304 1103 22-23 2.395525184415206 1103 24-25 1.64551245397438 1103 26-27 1.0667557216998418 1103 28-29 0.9838401302265787 1103 30-31 1.4311126357829167 1103 32-33 1.491895897690057 1103 34-35 1.59902789342717 1103 36-37 1.6359386528753532 1103 38-39 1.763522052464353 1103 40-41 1.64211021743235 1103 42-43 1.391728792254959 1103 44-45 1.7411440671547886 1103 46-47 1.3489281447850523 1103 48-49 2.324735368091069 1103 50-51 2.015168167761381 1103 52-53 1.6158897329943755 1103 54-55 1.3128625166403758 1103 56-57 1.0990679732562008 1103 58-59 1.077140604600313 1103 60-61 1.2949070919730516 1103 62-63 1.7120307442636857 1103 64-65 1.195160219615211 1103 66-67 1.4677702233837522 1103 68-69 1.283309109692258 1103 70-71 1.7071710476722153 1103 72-73 1.652626042499957 1103 74-75 1.534579074290015 1103 76-77 1.0977285285799638 1103 78-79 0.7525839139408141 1103 80-81 1.3386020339342082 1103 82-83 1.5474599604588803 1103 84-85 1.2090287363672623 1103 86-87 1.4851621668834696 1103 88-89 1.070758759204839 1103 90-91 0.9503044908681062 1103 92-93 1.1347103499738296 1103 94-95 1.5065709948054575 1103 96-97 1.2855024364374756 1103 98-99 1.4093941281568902 1103 100-101 1.0377499862424422 1103 102-103 0.808128080978026 1103 104-105 0.8473107023435915 1103 106-107 0.2876374646512936 1103 108-109 1.1255460195138909 1103 110-111 0.653668458161615 1103 112-113 0.681641620828195 1103 114-115 0.71136551792668 1103 116-117 0.7834068168460817 1103 118-119 0.7120605618863038 1103 120-121 0.5487117570346562 1103 122-123 0.032301556390478936 1103 124-125 -0.10906706365739183 1104 1 -0.01632487071162103 1104 2 0.04645069394673129 1104 3 0.7592228720516268 1104 4 0.4652853569704263 1104 5 -0.05793245740403563 1104 6 0.4838379295010107 1104 7 0.37018493591717316 1104 8 0.11196138430397085 1104 9 0.1991798631208681 1104 10-11 -0.10299343379352877 1104 12-13 0.1012067283259519 1104 14-15 0.3169466195749493 1104 16-17 -0.9514142131749388 1104 18-19 -0.3552873017969702 1104 20-21 -0.20505180081105223 1104 22-23 -0.09052800806000505 1104 24-25 0.09208830421995629 1104 26-27 -0.0732220375439745 1104 28-29 0.1855823227944562 1104 30-31 -0.3118676255459718 1104 32-33 -0.7445048066005526 1104 34-35 -0.6758307851012297 1104 36-37 -0.45700917401742913 1104 38-39 0.13447933167851644 1104 40-41 0.029238379789870095 1104 42-43 -0.18944996782288115 1104 44-45 -0.06828045327890564 1104 46-47 -0.5215657853419096 1104 48-49 -0.3378074916994933 1104 50-51 -0.21627470129616455 1104 52-53 -0.14673652963188744 1104 54-55 -0.11860815336240549 1104 56-57 -0.24209595965085384 1104 58-59 -0.3294018845109825 1104 60-61 -0.4309292531293494 1104 62-63 -0.15480272807437245 1104 64-65 -0.18928051803653645 1104 66-67 -0.4955325289098269 1104 68-69 -0.7098796587258711 1104 70-71 -0.8554274140088154 1104 72-73 -0.4940702785082891 1104 74-75 -0.49567762110429214 1104 76-77 -0.16670479548638895 1104 78-79 -0.1933432475826784 1104 80-81 0.17999764138486185 1104 82-83 -0.03311644580560369 1104 84-85 0.17015374795099092 1104 86-87 -0.09184645140955183 1104 88-89 -0.013524439757258477 1104 90-91 0.29522155138149486 1104 92-93 0.2557371315510686 1104 94-95 0.31914628903212616 1104 96-97 0.35658018641430544 1104 98-99 0.4593431597572959 1104 100-101 0.4816847466909664 1104 102-103 0.23964508588953137 1104 104-105 0.07518578342968496 1104 106-107 0.29949920128367324 1104 108-109 0.4917308031298653 1104 110-111 0.7107530657049388 1104 112-113 0.6245106783761507 1104 114-115 0.5909874250715248 1104 116-117 1.060767580445379 1104 118-119 1.0446988715888352 1104 120-121 1.060990507845517 1104 122-123 1.3268525711249808 1104 124-125 1.5389729697037389 1105 1 0.9309009921105726 1105 2 0.03081067647185165 1105 3 0.32864183229627386 1105 4 0.5992303110988644 1105 5 0.7374366994330117 1105 6 -0.36657709889566803 1105 7 -0.2145245442051511 1105 8 1.1707643550335476 1105 9 0.6875153808141832 1105 10-11 0.9323931979233784 1105 12-13 1.0869210140402394 1105 14-15 0.16827907916428586 1105 16-17 -0.06578730185995241 1105 18-19 0.1554160621173999 1105 20-21 -0.2523213508328972 1105 22-23 -0.7119784230884036 1105 24-25 -0.07728696882504238 1105 26-27 -0.48995423501011715 1105 28-29 0.3923538387402843 1105 30-31 0.10643731110759092 1105 32-33 0.6484615542557179 1105 34-35 0.7506873450866216 1105 36-37 0.1323311492678485 1105 38-39 0.8117183006606012 1105 40-41 0.31656909189122473 1105 42-43 0.5735469740731389 1105 44-45 0.22079774680846853 1105 46-47 0.19625859211549468 1105 48-49 0.4387324820881844 1105 50-51 -0.3766067240135129 1105 52-53 0.1342158513204943 1105 54-55 0.6526893564672136 1105 56-57 0.3637144379026651 1105 58-59 0.5220179494776573 1105 60-61 0.042814739880700614 1105 62-63 0.11116494339788474 1105 64-65 -0.007148582693591976 1105 66-67 0.02491308052660912 1105 68-69 -0.254353227970082 1105 70-71 0.09865733915851038 1105 72-73 0.11554090541481798 1105 74-75 0.1785906183015591 1105 76-77 0.4273100581614955 1105 78-79 0.03007309142998693 1105 80-81 -0.030662035329861226 1105 82-83 0.20012951312843086 1105 84-85 -0.3504229230843947 1105 86-87 -0.1589936772723739 1105 88-89 -0.031261442815363694 1105 90-91 -0.09009954953593535 1105 92-93 -0.4127643975009221 1105 94-95 -0.12921544098097826 1105 96-97 -0.24451199357695685 1105 98-99 -0.1985423797796173 1105 100-101 -0.011456362963905065 1105 102-103 -0.06214608929613874 1105 104-105 -0.29742234238945287 1105 106-107 -0.7830696060557756 1105 108-109 -0.3758969819291096 1105 110-111 -0.3526807481875913 1105 112-113 -0.30060946142288714 1105 114-115 -0.06266045609929449 1105 116-117 -0.12669419325492726 1105 118-119 -0.19313424330849926 1105 120-121 -0.12935462103172313 1105 122-123 -0.11185428776536455 1105 124-125 0.5402835856932562 1106 1 -0.9767981623175537 1106 2 -1.2275782865900062 1106 3 -1.3398939932488965 1106 4 -0.884881838433845 1106 5 -1.354597123441934 1106 6 -1.358655425553433 1106 7 -0.4494155099372392 1106 8 -1.4246072515530095 1106 9 -1.061216261375403 1106 10-11 -1.6276869088857033 1106 12-13 -1.2866437389637646 1106 14-15 -0.959268762402246 1106 16-17 -1.2692140931372116 1106 18-19 -0.5813440624931907 1106 20-21 -1.3590859258217698 1106 22-23 -1.0893705014150612 1106 24-25 -2.698960311059132 1106 26-27 -1.8547962465811025 1106 28-29 -1.6405344567648363 1106 30-31 -2.538867539804741 1106 32-33 -5.343750283750516 1106 34-35 -2.3100158146819574 1106 36-37 -1.6631739686671736 1106 38-39 -1.2622024826059715 1106 40-41 -1.5657629063286258 1106 42-43 -1.364147729976704 1106 44-45 -1.5128916167874387 1106 46-47 -1.3959977496735547 1106 48-49 -1.5572621774578757 1106 50-51 -1.3369983573023418 1106 52-53 -0.9598206417814197 1106 54-55 -1.3359621789132952 1106 56-57 -1.3350394561783325 1106 58-59 -1.0401538351240305 1106 60-61 -1.0115688827272216 1106 62-63 -1.1411270014174235 1106 64-65 -1.167673727330886 1106 66-67 -1.2097998700741925 1106 68-69 -1.0074017370933568 1106 70-71 -1.011133493062232 1106 72-73 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-2.5808687310429868 2216 120-121 -2.4260357177128213 2216 122-123 -2.174769150680227 2216 124-125 -2.331289285879615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6280.0 3 1558.0 4 2379.0 5 2584.0 6 2669.0 7 1283.0 8 814.0 9 706.0 10 702.0 11 707.0 12 703.0 13 668.0 14 640.0 15 565.0 16 495.0 17 460.0 18 420.0 19 390.0 20 328.0 21 326.0 22 401.0 23 757.0 24 1086.0 25 1047.0 26 1519.0 27 940.0 28 809.0 29 940.0 30 1125.0 31 1273.0 32 1560.0 33 1968.0 34 2816.0 35 3941.0 36 6337.0 37 10700.0 38 4500.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.79360202421832 26.51364540032532 9.276161214530996 15.416591360925358 2 14.914830489329336 38.051417985752366 29.719716252240314 17.314035272677984 3 12.22325973673906 50.540678936112535 23.160336154703455 14.075725172444953 4 6.9823483342370025 31.708235435869632 28.295379239713235 33.01403699018013 5 6.4024941261521775 59.8590276522682 25.0527140189168 8.685764202662812 6 17.677269715043074 53.52732092294716 14.351768178805951 14.443641183203807 7 18.93817305519994 54.315837035921376 15.528277731756909 11.21771217712177 8 20.47142104074102 51.26741471496348 14.377588673845922 13.883575570449583 9 20.39460802771293 35.49815498154982 13.666691768958506 30.44054522177875 10-11 15.760857287014934 48.18549449887417 20.479550571951414 15.574097642159485 12-13 17.03202777296654 49.35801372080939 19.702389469165833 13.907569037058234 14-15 17.321334437834174 48.68664809097071 17.880864522930946 16.111152948264177 16-17 14.572523740313725 48.963408664743845 20.595522286901975 15.868545308040455 18-19 14.573386550192033 47.31982830032382 22.692973868514198 15.413811280969952 20-21 17.556291889449508 45.74591460200316 22.990436026809245 13.707357481738084 22-23 17.41673387248732 45.423095186053345 22.595476231750794 14.56469470970854 24-25 16.393553731455683 45.661570901423296 23.45583251750885 14.489042849612169 26-27 15.256305020671581 45.957933896122476 23.64392165131673 15.14183943188921 28-29 15.744924393035726 44.27601060304838 24.722121814567146 15.256943189348757 30-31 15.607114886438941 45.93650219892765 24.113651424784628 14.342731489848786 32-33 15.393478424580165 46.42292341290759 23.889600120491 14.293998042021236 34-35 15.22143486478956 45.93389711807098 24.30474497902751 14.539923038111949 36-37 14.98411072789433 46.40571110140518 23.452866846393665 15.157311324306821 38-39 14.919685822081316 47.15681032600101 23.57991128916869 14.34359256274898 40-41 14.464726790770529 46.88610759684318 24.484155672028436 14.165009940357853 42-43 14.674297763385797 46.78213720912719 23.422697492281046 15.120867535205965 44-45 14.798554107990059 47.43956623239702 23.386550192032534 14.37532946758039 46-47 14.827923789441977 47.029896829580544 23.149333534151666 14.992845846825814 48-49 14.029142663503896 48.47772883015174 22.61982755374826 14.873300952596107 50-51 14.189860834990059 48.088740285559375 22.93963491776613 14.781763961684439 52-53 13.781807502014443 48.287911078311026 22.86826667469934 15.062014744975185 54-55 14.907636812735802 47.8631834988817 22.559510810220573 14.669668878161923 56-57 15.220157992635041 48.060486026914475 21.41485492239685 15.304501058053631 58-59 14.17641255807999 47.674917727858066 22.651384506480106 15.49728520758184 60-61 14.878155310560878 48.14371347671547 21.99379480691608 14.98433640580758 62-63 14.783381403860202 48.18398837270598 22.30572855088071 14.726901672553112 64-65 15.086865826750307 47.55141538206655 22.343381705085434 15.018337086097702 66-67 15.290485334538198 47.64486614706879 21.84268986031101 15.221958658082006 68-69 15.27165932452276 47.96415527693061 22.070861101698107 14.693324296848525 70-71 15.6475443167613 47.072156874557585 22.52662017862253 14.753678630058587 72-73 15.815354493768591 47.36096991603599 21.786211830264694 15.03746375993072 74-75 15.705528864255916 47.546575899513535 21.994216606171964 14.753678630058587 76-77 15.410450852078048 46.237376970171624 23.56675427168601 14.785417906064326 78-79 15.609774464400013 46.113935012613425 22.73579577544335 15.540494747543207 80-81 16.058828402099508 46.56796668499092 22.257197292025936 15.116007620883632 82-83 16.837103527214676 45.2144642910931 22.419349980421096 15.529082201271121 84-85 16.186847198270993 44.618315724473426 22.416091209627016 16.778745867628565 86-87 16.90224635334694 44.868328902878915 22.748940079672874 15.48048466410127 88-89 17.07318909882298 43.51961323262522 23.68724255043564 15.719955118116166 90-91 17.262828139825594 43.43343825775261 23.467174721749476 15.83655888067232 92-93 16.90977687076879 44.27793633700571 22.723336320438577 16.088950471786916 94-95 16.13714578328677 43.55199445753918 23.79718810479468 16.513671654379372 96-97 16.8789017493392 44.35474761470861 22.659326922352836 16.10702371359936 98-99 15.568474479268643 45.169962498305644 22.973176498938205 16.288386523487507 100-101 15.423136587496423 45.86653011431239 22.51532448755215 16.195008810639035 102-103 15.25931727300796 46.94218734703406 22.27635909060102 15.522136289356958 104-105 13.92382185942136 48.65882494691025 22.077051674021416 15.340301519646973 106-107 13.434443080584066 49.01689095057721 22.062156890799965 15.486509078038752 108-109 13.89552002771272 49.726261568931626 21.20173806959809 15.176480333757558 110-111 13.788377399411115 49.80910138335605 20.689343564796335 15.7131776524365 112-113 13.792895709864226 49.60577741296605 21.986851716581445 14.614475160588283 114-115 13.972875076246488 49.4709811511149 21.351276046177134 15.204867726461485 116-117 13.964591507082453 49.333172682295 21.318894821263168 15.383340989359379 118-119 14.214711729622268 48.42159166214832 21.349930718717992 16.013765889511415 120-121 14.477310721869966 48.31995421479886 21.480639185505368 15.722095877825806 122-123 14.975186945094999 47.95584104583826 21.027463797037495 16.04150821202925 124-125 14.60242633271332 48.09515561814252 21.0372534696859 16.265164579458254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1900.0 1 2072.0 2 2527.5 3 2241.0 4 1352.5 5 830.0 6 592.5 7 586.5 8 579.5 9 565.0 10 500.0 11 369.5 12 300.5 13 265.0 14 242.0 15 241.0 16 245.0 17 219.0 18 214.5 19 242.0 20 251.5 21 261.0 22 278.0 23 298.0 24 339.5 25 454.5 26 576.0 27 666.5 28 778.0 29 960.0 30 1187.5 31 1331.0 32 1611.0 33 2108.0 34 2398.0 35 2664.0 36 3213.5 37 3578.5 38 3185.0 39 2810.0 40 2677.5 41 2339.5 42 1867.0 43 1694.5 44 1744.0 45 1664.0 46 1371.0 47 1049.5 48 1083.0 49 1011.0 50 898.5 51 723.5 52 519.0 53 406.0 54 323.5 55 311.5 56 324.5 57 303.0 58 224.5 59 180.5 60 202.0 61 215.5 62 166.5 63 120.5 64 102.5 65 91.0 66 89.5 67 75.0 68 61.0 69 62.0 70 64.0 71 53.5 72 48.5 73 62.0 74 55.0 75 26.5 76 13.5 77 18.0 78 19.0 79 12.5 80 9.5 81 6.5 82 5.5 83 4.5 84 4.5 85 3.5 86 1.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030121389198469834 2 0.0015060694599234917 3 0.0 4 0.0030121389198469834 5 0.0030121389198469834 6 0.0030121389198469834 7 0.004518208379770476 8 0.004518208379770476 9 0.004518208379770476 10-11 0.003765173649808729 12-13 0.003765173649808729 14-15 0.004518208379770476 16-17 0.003765173649808729 18-19 0.004518208379770476 20-21 0.004518208379770476 22-23 0.08961113286544775 24-25 0.004518208379770476 26-27 0.003765173649808729 28-29 0.0030121389198469834 30-31 0.0030121389198469834 32-33 0.004518208379770476 34-35 0.002259104189885238 36-37 0.0015060694599234917 38-39 0.003765173649808729 40-41 0.0030121389198469834 42-43 0.004518208379770476 44-45 0.004518208379770476 46-47 0.004518208379770476 48-49 7.530347299617459E-4 50-51 0.0030121389198469834 52-53 0.003765173649808729 54-55 0.003765173649808729 56-57 0.003765173649808729 58-59 0.003765173649808729 60-61 0.0030121389198469834 62-63 0.003765173649808729 64-65 0.003765173649808729 66-67 7.530347299617459E-4 68-69 7.530347299617459E-4 70-71 0.0015060694599234917 72-73 7.530347299617459E-4 74-75 0.0015060694599234917 76-77 0.002259104189885238 78-79 7.530347299617459E-4 80-81 0.002259104189885238 82-83 0.0 84-85 0.002259104189885238 86-87 0.002259104189885238 88-89 0.002259104189885238 90-91 0.0015060694599234917 92-93 0.002259104189885238 94-95 0.002259104189885238 96-97 0.002259104189885238 98-99 0.0015060694599234917 100-101 0.0015060694599234917 102-103 0.003765173649808729 104-105 0.0015060694599234917 106-107 0.002259104189885238 108-109 0.003765173649808729 110-111 0.002259104189885238 112-113 0.002259104189885238 114-115 0.002259104189885238 116-117 0.002259104189885238 118-119 0.0030121389198469834 120-121 0.0015060694599234917 122-123 0.002259104189885238 124-125 0.002259104189885238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 66398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.89581011476249 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37548795757532 31.234374529353293 2 7.744715327391913 6.334528148438206 3 4.53708477572365 5.5664327238772255 4 2.732562421742653 4.4700141570529235 5 1.9518303012447522 3.991084068797253 6 1.4178389924136408 3.4790204524232657 7 0.9427708624880312 2.698876472182897 8 0.7181262429108051 2.3494683574806468 9 0.47506812992560954 1.748546642971174 >10 2.883553067688002 21.550347902045242 >50 0.15467334462694263 4.040784360974728 >100 0.047875082860720335 3.596493870297298 >500 0.011048096044781616 3.085936323383234 >1k 0.007365397363187744 5.854091990722613 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2808 4.229043043465165 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1079 1.6250489472574476 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 1.1687099009006294 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 702 1.0572607608662912 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 571 0.8599656616163137 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 324 0.4879665050152113 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 321 0.4834482966354408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.45935118527666496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.3388656284827856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.30573210036446885 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.22892255790837074 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.22892255790837074 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.22741648844844725 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 128 0.19277689087020694 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 112 0.16867977951143107 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 107 0.16114943221181363 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 105 0.15813729329196663 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.15512515437211963 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 89 0.13404018193319076 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.13102804301334378 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 87 0.13102804301334378 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 84 0.1265098346335733 No Hit GAACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.1250037651736498 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 78 0.11747341787403236 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 78 0.11747341787403236 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 76 0.11446127895418537 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 72 0.10843700111449141 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.1069309316545679 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 69 0.10391879273472092 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 69 0.10391879273472092 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 69 0.10391879273472092 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 68 0.10241272327479745 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 67 0.10090665381487395 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0015060694599234917 0.0 22-23 0.0 0.0 0.0 0.0022591041898852374 0.0 24-25 0.0 0.0 0.0 0.003765173649808729 0.0 26-27 0.0 0.0 0.0 0.009789451489502696 0.0 28-29 0.0 0.0 0.0 0.019578902979005393 0.0 30-31 0.0 0.0 0.0 0.04970029217747523 0.0 32-33 0.0 0.0 0.0 0.10467182746468268 0.0 34-35 0.0 0.0 0.0 0.1378053555829995 0.0 36-37 0.0 0.0 0.0 0.17846923100093376 0.0 38-39 0.0 0.0 0.0 0.24548932196752915 0.0 40-41 0.0 0.0 0.0 0.3027199614446218 0.0 42-43 0.0 0.0 0.0 0.36446880930148495 0.0 44-45 0.0 0.0 0.0 0.42471158769842465 0.0 46-47 0.0 0.0 0.0 0.5075454079942167 0.0 48-49 0.0 0.0 0.0 0.5979095755896262 0.0 50-51 0.0 0.0 0.0 0.6747191180457243 0.0 52-53 0.0 0.0 0.0 0.7696014940209042 0.0 54-55 0.0 0.0 0.0 0.8690020783758547 0.0 56-57 0.0 0.0 0.0 0.9699087321907287 0.0 58-59 0.0 0.0 0.0 1.0994307057441488 0.0 60-61 0.0 0.0 0.0 1.229705714027531 0.0 62-63 0.0 0.0 0.0 1.351697340281334 0.0 64-65 0.0 0.0 0.0 1.466911653965481 0.0 66-67 0.0 0.0 0.0 1.6084821831982892 0.0 68-69 0.0 0.0 0.0 1.7869514141992229 0.0 70-71 0.0 0.0 0.0 1.9684327841200036 0.0 72-73 0.0 0.0 0.0 2.145395945661014 0.0 74-75 0.0 0.0 0.0 2.3426910449109912 0.0 76-77 0.0 0.0 0.0 2.533961866321275 0.0 78-79 0.0 0.0 0.0 2.7576131811199134 0.0 80-81 0.0 0.0 0.0 2.995572155787825 0.0 82-83 0.0 0.0 0.0 3.213952227476731 0.0 84-85 0.0 0.0 0.0 3.5242025362209706 0.0 86-87 0.0 0.0 0.0 3.8141209072562425 0.0 88-89 0.0 0.0 0.0 4.12361818127052 0.0 90-91 0.0 0.0 0.0 4.479050573812464 0.0 92-93 0.0 0.0 0.0 4.762944667008043 0.0 94-95 0.0 0.0 0.0 5.080725323051899 0.0 96-97 0.0 0.0 0.0 5.442181993433538 0.0 98-99 0.0 0.0 0.0 5.862375372752192 0.0 100-101 0.0 0.0 0.0 6.2358805988132175 0.0 102-103 0.0 0.0 0.0 6.683936263140456 0.0 104-105 0.0 0.0 0.0 7.144793517877044 0.0 106-107 0.0 0.0 0.0 7.558209584626043 0.0 108-109 0.0 0.0 0.0 7.957317991505768 0.0 110-111 0.0 0.0 0.0 8.483689267749028 0.0 112-113 0.0 0.0 0.0 9.068044218199343 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTGTG 20 8.44912E-4 89.242096 7 CTGTGCG 20 8.44912E-4 89.242096 9 GAACAAA 335 0.0 88.798096 1 GGAATCA 25 0.00204901 71.39367 1 CCTGTGC 25 0.00204901 71.39367 8 GGAAACA 90 0.0 66.10525 1 GAAAACA 245 0.0 63.137257 1 CTAGATA 30 0.0042204848 59.494728 4 GCGAATG 20 1.7670209E-4 59.494728 80-81 ACGTTAA 20 1.7670209E-4 59.494728 104-105 GGATCTG 15 0.004229794 59.494728 30-31 GCTGCGT 20 1.7670209E-4 59.494728 92-93 CGCGAAT 20 1.7670209E-4 59.494728 78-79 TAACGCG 20 1.7670209E-4 59.494728 76-77 CTGCGTA 20 1.7670209E-4 59.494728 92-93 TCTACGT 20 1.7670209E-4 59.494728 100-101 CTACGTT 20 1.7670209E-4 59.494728 102-103 ATATAGG 40 1.7605486E-4 59.494728 2 AACGCGA 20 1.7670209E-4 59.494728 76-77 AGGGGTC 40 1.7605486E-4 59.494728 5 >>END_MODULE