FastQCFastQC Report
Thu 26 May 2016
SRR936274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66398
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34125.138708997258953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34085.1326847194192595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28544.298322238621645No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9451.4232356396276997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8831.329859333112443No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7271.0949124973643785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6891.0376818578872857No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.8780384951353957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.5948974366697792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1430.2153679327690593No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.20633151600951838No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.1972950992499774No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1300.19578902979005391No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA1120.16867977951143107No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1080.1626555016717371No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG1050.15813729329196663No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA950.1430765986927317No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT930.14006445977288473No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.13705232085303776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC910.13705232085303776No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG870.13102804301334378No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG860.12952197355342027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA800.12048555679387933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.12048555679387933No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG790.11897948733395583No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA750.11295520949426187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG740.1114491400343384No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT690.10391879273472092No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA680.10241272327479745No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC670.10090665381487395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.090.981031
CTGTCAG250.002048340671.399555
CCAATAC508.801362E-871.399553
CAATACT603.8908183E-969.416224
ATGGGAG359.089438E-567.999575
ATATAGG359.089438E-567.999572
GCTTACT359.089438E-567.999578
CTTACTA359.089438E-567.999579
GTTATCA507.3424944E-659.54451
ATACTTA507.3754436E-659.499636
CATGGGG805.6024874E-1059.4996224
CTCTGTG150.00422841759.49962280-81
AGGCTTA401.7598324E-459.4996226
GTATCAA13600.054.2496572
TACTTAT551.297284E-554.090577
TCATTAA350.007764301350.9996768
CCCTTGA350.007764301350.9996769
CTTCTGT350.007764301350.9996762
TTAGCCT302.1828972E-549.5830228-29
TGGGAGG505.296295E-447.5997056
Produced by FastQC (version 0.11.5)