##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66398 Sequences flagged as poor quality 0 Sequence length 125 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.364920630139462 31.0 31.0 34.0 30.0 34.0 2 31.162730805144733 31.0 31.0 34.0 28.0 34.0 3 31.433627518901172 31.0 31.0 34.0 28.0 34.0 4 35.39483116961354 37.0 35.0 37.0 33.0 37.0 5 35.13095273954035 37.0 35.0 37.0 33.0 37.0 6 35.39978613813669 37.0 35.0 37.0 33.0 37.0 7 35.19068345432091 37.0 35.0 37.0 32.0 37.0 8 35.30824723636254 37.0 35.0 37.0 32.0 37.0 9 36.87909274375734 39.0 37.0 39.0 32.0 39.0 10-11 36.77524925449562 39.0 37.0 39.0 32.0 39.0 12-13 36.69077381848851 39.0 37.0 39.0 32.0 39.0 14-15 37.3248441218109 39.0 36.0 41.0 32.0 41.0 16-17 37.376208620741586 39.0 36.5 41.0 32.0 41.0 18-19 37.859822585017625 39.5 37.0 41.0 32.5 41.0 20-21 37.978049037621616 40.0 37.0 41.0 33.0 41.0 22-23 37.71594023916383 40.0 36.5 41.0 32.0 41.0 24-25 37.55358595138408 40.0 36.0 41.0 32.0 41.0 26-27 37.13702972981114 40.0 35.0 41.0 31.5 41.0 28-29 36.809098165607395 39.5 35.0 41.0 31.0 41.0 30-31 36.00747010452122 37.5 35.0 40.0 30.5 41.0 32-33 35.060762372360614 36.0 34.5 40.0 30.0 41.0 34-35 34.45135395644447 35.0 34.0 40.0 26.5 41.0 36-37 33.957265279074676 35.0 33.0 40.0 24.0 41.0 38-39 33.7106539353595 35.0 33.0 40.0 22.0 41.0 40-41 33.353881140998226 35.0 33.0 40.0 19.5 41.0 42-43 32.9113150998524 35.0 33.0 40.0 15.5 41.0 44-45 32.22913340763276 35.0 33.0 40.0 5.0 41.0 46-47 31.08579324678454 35.0 30.5 40.0 2.0 41.0 48-49 30.27139371667821 35.0 27.0 40.0 2.0 41.0 50-51 30.17242236211934 35.0 27.5 40.0 2.0 41.0 52-53 30.212702189824995 35.0 28.5 40.0 2.0 41.0 54-55 30.131555167324315 35.0 29.0 40.0 2.0 41.0 56-57 29.973545889936446 35.0 28.5 40.0 2.0 41.0 58-59 29.79296816169162 35.0 28.0 40.0 2.0 41.0 60-61 29.606516762553092 35.0 27.5 40.0 2.0 41.0 62-63 29.392963643483235 35.0 27.0 39.0 2.0 41.0 64-65 29.157821018705384 35.0 27.0 39.0 2.0 41.0 66-67 28.894085665230882 35.0 26.0 39.0 2.0 41.0 68-69 28.582366938763215 35.0 26.0 38.0 2.0 40.5 70-71 28.252018133076298 35.0 26.0 37.5 2.0 40.0 72-73 27.893626314045605 35.0 25.0 37.0 2.0 40.0 74-75 27.544180547606857 35.0 24.0 37.0 2.0 39.0 76-77 27.154432362420554 35.0 22.0 36.0 2.0 39.0 78-79 26.788909304497125 34.5 20.0 36.0 2.0 39.0 80-81 26.432618452363023 34.0 19.0 35.5 2.0 37.5 82-83 26.003516672188923 34.0 10.5 35.0 2.0 37.0 84-85 25.598685201361484 34.0 3.5 35.0 2.0 37.0 86-87 25.236957438477063 34.0 2.0 35.0 2.0 36.0 88-89 24.900343383836862 34.0 2.0 35.0 2.0 36.0 90-91 24.53842736226995 33.5 2.0 35.0 2.0 36.0 92-93 24.20003614566704 33.0 2.0 35.0 2.0 35.5 94-95 23.901676255308892 33.0 2.0 35.0 2.0 35.0 96-97 23.629506912858822 33.0 2.0 35.0 2.0 35.0 98-99 23.32562727793006 33.0 2.0 35.0 2.0 35.0 100-101 23.046808638814422 33.0 2.0 35.0 2.0 35.0 102-103 22.033382029579204 31.0 2.0 34.0 2.0 35.0 104-105 22.39587035754089 32.0 2.0 35.0 2.0 35.0 106-107 22.279338233079308 33.0 2.0 35.0 2.0 35.0 108-109 22.04450435254074 32.5 2.0 35.0 2.0 35.0 110-111 21.828232778095725 32.0 2.0 35.0 2.0 35.0 112-113 21.562358805988133 32.0 2.0 35.0 2.0 35.0 114-115 21.344212175065515 32.0 2.0 35.0 2.0 35.0 116-117 21.0748591825055 32.0 2.0 35.0 2.0 35.0 118-119 20.857970119581914 32.0 2.0 35.0 2.0 35.0 120-121 20.58559745775475 31.0 2.0 35.0 2.0 35.0 122-123 20.319452393144374 31.0 2.0 35.0 2.0 35.0 124-125 18.942686526702612 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.026396982562186366 1101 2 0.9212076999393624 1101 3 0.1453282757444576 1101 4 0.2542561229657281 1101 5 0.3701012106888939 1101 6 0.4127814444423592 1101 7 0.21428140331136802 1101 8 0.6782919929429454 1101 9 0.08988100339412597 1101 10-11 0.16043181686276853 1101 12-13 0.49779189749699526 1101 14-15 0.6347143835859157 1101 16-17 0.7916383462737357 1101 18-19 0.8232566106556405 1101 20-21 0.519388808733261 1101 22-23 0.4083340139443479 1101 24-25 0.11854945177726961 1101 26-27 0.42018068311839585 1101 28-29 0.8183598658300326 1101 30-31 0.0141919609292529 1101 32-33 -0.4701606227345039 1101 34-35 -0.40945694811593825 1101 36-37 -0.36509675160719723 1101 38-39 -0.4201452426505199 1101 40-41 -0.49325258867227006 1101 42-43 -0.7427724147535244 1101 44-45 -0.6097446022810118 1101 46-47 -0.14803154173115374 1101 48-49 -0.7825286801311471 1101 50-51 -0.7488407422066494 1101 52-53 -0.9536954991491875 1101 54-55 -0.2533485646657425 1101 56-57 -0.5551361546201505 1101 58-59 -0.12637904331100813 1101 60-61 -0.13428750844745352 1101 62-63 -0.20211345707046746 1101 64-65 -0.30770151049958017 1101 66-67 -0.35189150084958243 1101 68-69 0.07203034850856938 1101 70-71 0.29919186741538084 1101 72-73 0.4924365024690722 1101 74-75 0.32668876794409485 1101 76-77 0.505708867055187 1101 78-79 0.8426803766257187 1101 80-81 0.9247237245896613 1101 82-83 0.5001458690042 1101 84-85 0.44096660015059896 1101 86-87 0.06360741880522269 1101 88-89 -0.1393024980893074 1101 90-91 0.0037733648708950795 1101 92-93 0.02438643343041136 1101 94-95 0.06874659886916845 1101 96-97 -0.20732840561473154 1101 98-99 -0.3555820614591063 1101 100-101 -0.026849899583709202 1101 102-103 0.047718400189030064 1101 104-105 0.1321293286947416 1101 106-107 0.14450034150272018 1101 108-109 0.6150467171111877 1101 110-111 0.8313926008623049 1101 112-113 0.8150535944698163 1101 114-115 1.0678615548525237 1101 116-117 0.8936004193909 1101 118-119 1.5952088238221016 1101 120-121 1.598518811754289 1101 122-123 1.2235677944016992 1101 124-125 1.2427759698597853 1102 1 0.272753502874707 1102 2 0.8380766319782005 1102 3 0.2626999817083977 1102 4 0.1744190910655945 1102 5 0.7379063424503371 1102 6 0.2160061323757816 1102 7 0.35384451010748563 1102 8 0.12316023150050626 1102 9 0.3860841934079957 1102 10-11 0.6528902079862107 1102 12-13 0.6547353787730046 1102 14-15 0.7923080035581762 1102 16-17 0.22749132824322515 1102 18-19 0.6352798422783863 1102 20-21 0.7559959515903998 1102 22-23 0.7456259695615444 1102 24-25 0.8055726834000154 1102 26-27 0.4902222919949608 1102 28-29 0.668091141835589 1102 30-31 0.44003280697641145 1102 32-33 0.32153493898533014 1102 34-35 0.9542306385692143 1102 36-37 0.9737555368810149 1102 38-39 0.8527829820374109 1102 40-41 0.38855219634853455 1102 42-43 0.4490618432215143 1102 44-45 0.5959159386343771 1102 46-47 0.29757352068216036 1102 48-49 0.4357878940713462 1102 50-51 0.6812476766560458 1102 52-53 0.9197875105595514 1102 54-55 0.9011243895644938 1102 56-57 1.1086121116766599 1102 58-59 1.4468265738873285 1102 60-61 0.9849912710254962 1102 62-63 1.167685433359491 1102 64-65 0.8554833022604775 1102 66-67 0.8921705657246193 1102 68-69 0.8166125260397763 1102 70-71 0.40369082719346494 1102 72-73 0.4351376120391137 1102 74-75 0.19700776933105857 1102 76-77 -0.49151720784079345 1102 78-79 -0.3787135207390513 1102 80-81 -0.5533449300566602 1102 82-83 -0.7064248660859498 1102 84-85 -0.83876640955814 1102 86-87 -0.8045444535942252 1102 88-89 -0.6236991693791865 1102 90-91 -0.41417913166170095 1102 92-93 -0.21625156934351253 1102 94-95 -0.030291195860371545 1102 96-97 -0.020218488832483672 1102 98-99 0.011832987084581248 1102 100-101 -0.029580482107981254 1102 102-103 0.17189489117377477 1102 104-105 0.41298924547698945 1102 106-107 0.16435124302837778 1102 108-109 0.09870656454531002 1102 110-111 -0.12305434782008362 1102 112-113 -0.8851891239767866 1102 114-115 -0.9307081400157138 1102 116-117 -0.8837054058518206 1102 118-119 -0.7333279306855296 1102 120-121 -0.7333986639738654 1102 122-123 -0.7301856730046801 1102 124-125 -0.5864109233440971 1103 1 0.13070804832925376 1103 2 0.8563847127862836 1103 3 0.1716105156189336 1103 4 0.37183972598622717 1103 5 0.17955497159896794 1103 6 0.3276583690280219 1103 7 0.36520814647111877 1103 8 0.2952381535784241 1103 9 0.1295004993243012 1103 10-11 0.6170857346817371 1103 12-13 0.31450269479032045 1103 14-15 0.6615359977861743 1103 16-17 0.21053606128795366 1103 18-19 0.19611137185992078 1103 20-21 -0.2583889690445176 1103 22-23 -0.11142669999112087 1103 24-25 -0.4227372011598618 1103 26-27 -0.753644951872289 1103 28-29 -0.22336809337365082 1103 30-31 -0.07029367210007109 1103 32-33 -0.1128986829482912 1103 34-35 0.32350372659229976 1103 36-37 0.18001455564004232 1103 38-39 0.1278100383144647 1103 40-41 -0.1661465771002426 1103 42-43 0.038437745097418485 1103 44-45 -0.42415080018235685 1103 46-47 0.10939711375575456 1103 48-49 0.3029866675201198 1103 50-51 1.0629666521250165 1103 52-53 0.6435880156100566 1103 54-55 0.9720118354519407 1103 56-57 0.6038321693967212 1103 58-59 0.734119142429897 1103 60-61 0.2238080098422408 1103 62-63 -0.1102727195986617 1103 64-65 0.43011353314070533 1103 66-67 0.49845664076069696 1103 68-69 0.7722853254625726 1103 70-71 0.948108215360854 1103 72-73 1.218777583179083 1103 74-75 0.9452040175273062 1103 76-77 0.9644712481476674 1103 78-79 0.8804856134600847 1103 80-81 1.3845158203040882 1103 82-83 1.358780761619677 1103 84-85 1.4194406611489327 1103 86-87 1.1437780572282854 1103 88-89 1.5700237743437597 1103 90-91 1.4136978524652868 1103 92-93 1.0398811867892448 1103 94-95 1.3009227291035508 1103 96-97 1.848423286059294 1103 98-99 1.4288153103169066 1103 100-101 1.5048260835485863 1103 102-103 1.678884429413312 1103 104-105 2.0010123335000785 1103 106-107 2.4167863079634415 1103 108-109 1.9824096669984108 1103 110-111 2.009386128370398 1103 112-113 1.9595078457201822 1103 114-115 1.883640705583133 1103 116-117 2.0285420688956535 1103 118-119 2.011801939444343 1103 120-121 1.3366770936018924 1103 122-123 1.5718074727384668 1103 124-125 1.3179451372619653 1104 1 0.2366296496636977 1104 2 0.5737417695928784 1104 3 -0.04644156481846906 1104 4 -0.084837134360356 1104 5 0.20309310522884516 1104 6 0.32691701048849353 1104 7 0.49948671194234606 1104 8 0.1726359851046979 1104 9 0.4670966442598967 1104 10-11 0.47177946093509604 1104 12-13 0.31630975623041024 1104 14-15 0.485787689010337 1104 16-17 0.35167219324977594 1104 18-19 0.0951012708498169 1104 20-21 0.22122367111858665 1104 22-23 -0.3422672152353101 1104 24-25 -0.027037080689105153 1104 26-27 0.35195230510111486 1104 28-29 -0.43442360259429336 1104 30-31 -0.07942164911795402 1104 32-33 0.5064137070063026 1104 34-35 0.5552709400108924 1104 36-37 0.37160940319819247 1104 38-39 0.0833285259430454 1104 40-41 0.13220390014932804 1104 42-43 0.11034952343121063 1104 44-45 0.43786384164879166 1104 46-47 0.6755633634082407 1104 48-49 1.2661040801788168 1104 50-51 1.2919537710203954 1104 52-53 1.074317327073313 1104 54-55 0.9304494223298931 1104 56-57 0.46681984431100076 1104 58-59 0.42376792382179573 1104 60-61 0.017756670763375837 1104 62-63 0.5512044372913394 1104 64-65 0.08389909518314553 1104 66-67 0.274010342919901 1104 68-69 0.8267288432088442 1104 70-71 0.6536498704437932 1104 72-73 0.8125131035201072 1104 74-75 0.6858675334201791 1104 76-77 0.5550096597869931 1104 78-79 1.1558539759844102 1104 80-81 0.7960161446484477 1104 82-83 0.28218915750513673 1104 84-85 0.5413941409005751 1104 86-87 0.2364942095774687 1104 88-89 0.35762455277022553 1104 90-91 0.09667440700147267 1104 92-93 -0.17103531770524327 1104 94-95 0.33270847648562807 1104 96-97 0.5309364915082782 1104 98-99 0.2753077577267824 1104 100-101 0.29696768302963505 1104 102-103 0.18782706679631644 1104 104-105 0.20525661113885363 1104 106-107 0.24831257985668742 1104 108-109 0.44024490006300354 1104 110-111 0.327428941826053 1104 112-113 0.696232894371839 1104 114-115 1.009208308214955 1104 116-117 1.1501248693775352 1104 118-119 1.0964558176762047 1104 120-121 1.5286426204298067 1104 122-123 1.7674797922639982 1104 124-125 1.7389904528026925 1105 1 -0.0242703066491039 1105 2 0.2898623462639165 1105 3 0.1615095055179232 1105 4 0.17501432916083104 1105 5 0.6265968186408131 1105 6 0.34814898951864137 1105 7 0.09074927201224625 1105 8 0.3160173743576493 1105 9 0.47120324102704103 1105 10-11 -8.598248997770952E-5 1105 12-13 0.3841996644872907 1105 14-15 0.7244508607010403 1105 16-17 0.5536818044337011 1105 18-19 0.7522441279926753 1105 20-21 0.2860554753999267 1105 22-23 -0.08473117329559443 1105 24-25 0.08087030244763582 1105 26-27 0.028317530090191667 1105 28-29 -0.516730286735843 1105 30-31 -0.2674076692140659 1105 32-33 -0.07667934672895882 1105 34-35 -0.3597574566688806 1105 36-37 0.02792220354768915 1105 38-39 -0.042752732248303005 1105 40-41 -0.5736501846038422 1105 42-43 -0.4634381567784871 1105 44-45 -0.21390548993704783 1105 46-47 -0.6170098126511725 1105 48-49 -1.0037954392619852 1105 50-51 -0.8791689900106228 1105 52-53 -0.9138434418214025 1105 54-55 -0.13578037234026752 1105 56-57 -0.534840269275719 1105 58-59 -0.343947235636481 1105 60-61 -0.1150087289744981 1105 62-63 -0.16207647140241477 1105 64-65 -0.20076669773952815 1105 66-67 0.25199199429604846 1105 68-69 0.20619585937310703 1105 70-71 -0.6638686966160563 1105 72-73 -0.16188619748469435 1105 74-75 -0.3125160401927545 1105 76-77 -0.824850541174122 1105 78-79 -0.5775230445485775 1105 80-81 -0.2658449300566623 1105 82-83 -0.1546391518002359 1105 84-85 -0.8465045047962363 1105 86-87 -0.5146635012132705 1105 88-89 -0.5927467884268047 1105 90-91 -0.7228100840426528 1105 92-93 -0.6257753788673206 1105 94-95 -0.5049935768127511 1105 96-97 -0.4788494412134341 1105 98-99 -0.31822653672494283 1105 100-101 -0.24178286306036156 1105 102-103 -0.016497965969083594 1105 104-105 0.06060829309603477 1105 106-107 0.02804171921885512 1105 108-109 0.056147040735783804 1105 110-111 0.8474813664656331 1105 112-113 0.5326680188803579 1105 114-115 0.5142323361747643 1105 116-117 0.45855649891008454 1105 118-119 0.43691016455256815 1105 120-121 0.46005371697851416 1105 122-123 0.47368337461437093 1105 124-125 0.8335295528463789 1106 1 -0.012877338246788383 1106 2 0.28959532356698503 1106 3 0.27054377343069547 1106 4 -0.2377137527261297 1106 5 0.14453184311789613 1106 6 0.19247475720889184 1106 7 0.22417161291122056 1106 8 0.10747264805591072 1106 9 -0.21780432461603993 1106 10-11 -0.3134816211192586 1106 12-13 -0.17374426074635352 1106 14-15 0.2500850396062475 1106 16-17 -0.2080894461225924 1106 18-19 -0.2834034020473766 1106 20-21 -0.18621866790225283 1106 22-23 -0.19955093297517124 1106 24-25 -0.058204018869673746 1106 26-27 -0.6853451312360193 1106 28-29 -0.6850880971497304 1106 30-31 -0.3749288085109086 1106 32-33 -1.277346903471276 1106 34-35 -1.195093460229181 1106 36-37 -1.3507604844808014 1106 38-39 -1.137412278309725 1106 40-41 -0.42207030031367765 1106 42-43 -0.1992191981670075 1106 44-45 -0.7253874659050048 1106 46-47 -0.9703253444713766 1106 48-49 -1.8336129737524196 1106 50-51 -2.2803260883639815 1106 52-53 -1.940901741776898 1106 54-55 -1.4151306171110711 1106 56-57 -1.9452096506731387 1106 58-59 -1.7870268974077348 1106 60-61 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 23.0 5 85.0 6 205.0 7 358.0 8 497.0 9 726.0 10 1032.0 11 1236.0 12 1385.0 13 1734.0 14 2326.0 15 1842.0 16 835.0 17 699.0 18 741.0 19 752.0 20 832.0 21 900.0 22 1015.0 23 1047.0 24 1166.0 25 1286.0 26 1334.0 27 1343.0 28 1472.0 29 1674.0 30 1898.0 31 2028.0 32 2188.0 33 2664.0 34 3413.0 35 4848.0 36 6113.0 37 11302.0 38 5376.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.391065830721004 18.07029177718833 16.5872920183265 13.951350373764168 2 19.497575228169524 27.222205488117112 36.26012831711798 17.02009096659538 3 15.050152113015452 32.68321334979969 39.05238109581614 13.214253441368715 4 8.640320491581072 22.53682339829513 36.20741588602066 32.61544022410314 5 5.946353224834238 44.345991561181435 36.31856540084388 13.389089813140446 6 19.967468899665654 44.46368866532124 16.685743546492365 18.88309888852074 7 16.06825506792373 41.76933040151812 24.875749269556312 17.286665261001836 8 27.323112141931986 33.11545528479773 20.818398144522426 18.743034428747855 9 23.875719148167114 10.943100695804091 22.601584385071842 42.579595770956956 10-11 22.228832193740775 28.418777674026323 28.82541642820567 20.526973704027228 12-13 26.847947227326124 31.44522425374258 30.089008705081476 11.617819813849815 14-15 21.563149492454592 29.31940721106057 35.859513840778334 13.257929455706496 16-17 12.07792403385644 28.961715714328744 38.80463266965872 20.15572758215609 18-19 11.670532244947136 20.909515346847797 51.42172957016777 15.99822283803729 20-21 12.652489532817254 20.49233410644899 55.57095093225699 11.284225428476761 22-23 12.245097743907948 20.0570800325311 55.378174041386785 12.319648182174161 24-25 11.91828067110455 20.810867797222805 55.74113678122835 11.52971475044429 26-27 11.358022832013011 20.840989186421275 55.701978975270336 12.09900900629537 28-29 11.890418386095966 19.084159161420526 56.58905388716528 12.436368565318233 30-31 12.074911894936594 20.176059519865056 56.05063405524263 11.698394529955722 32-33 11.994337178830687 20.77246302599476 55.2832916654116 11.949908129762944 34-35 12.248862917557759 20.383897105334498 55.25467634567306 12.112563631434682 36-37 12.45745353775716 20.279225277869813 54.05735112503388 13.205970059339137 38-39 12.468749058706589 20.929847284556764 53.50537666797193 13.096026988764722 40-41 11.764661586192355 21.088737612578694 53.94063074188982 13.205970059339137 42-43 12.287360216875635 21.33739975149667 52.17214503558117 14.20309499604654 44-45 12.995345894898557 21.35089542572259 50.865302064976724 14.78845661440212 46-47 13.331726677762632 21.587425911839798 49.62532290011372 15.45552451028385 48-49 13.687230773865108 22.98701689911739 47.21736903937103 16.108383287646475 50-51 13.818915723440947 23.06742275339072 46.541505697008034 16.5721558261603 52-53 13.434544249653593 23.109825893126093 46.53593589975299 16.919693957467317 54-55 14.314330898411026 23.08080427743053 45.972588297311546 16.6322765268469 56-57 14.52293094359515 23.298441147676783 45.28202424881392 16.89660365991415 58-59 14.251396900462371 22.178712893654833 46.194858201424765 17.37503200445803 60-61 14.873300952596107 23.347264580744756 45.43619865205768 16.343235814601453 62-63 15.344704243382656 22.791520765089047 45.97688165970104 15.886893331827254 64-65 15.545130051056525 22.450562525415304 46.01111496001325 15.99319246351492 66-67 15.486392457490549 22.847417805021312 45.328102173290965 16.338087564197178 68-69 15.559956625199552 22.551131058164405 45.637669809331605 16.251242507304436 70-71 15.48349687107001 22.13219070282319 45.83449428810253 16.549818138004262 72-73 15.428291727851198 22.432320493994503 45.163598027034155 16.97578975112015 74-75 14.306261530931137 22.969238299634775 45.22685342068602 17.497646748748068 76-77 13.846153846153847 22.6860951090026 46.433977182875864 17.033773861967695 78-79 13.440916895087202 23.267267086358025 45.584957378234286 17.70685864032049 80-81 13.57129409992846 23.456455438834293 45.020520350916826 17.95173011032042 82-83 14.396626379005234 22.585940735720474 44.42938363643209 18.5880492488422 84-85 13.793337148709298 23.074490195487815 43.69709931022019 19.435073345582698 86-87 13.92371700741745 23.33747505553673 43.96400466885048 18.77480326819534 88-89 14.61361206078588 22.401614530776992 43.879241531996925 19.105531876440203 90-91 14.1046591311289 22.92741334257077 43.77037946277289 19.197548063527446 92-93 14.245911021416308 23.13247386969487 42.70911774451038 19.912497364378446 94-95 13.95856709314497 23.258756109132257 42.53160934687823 20.25106745084455 96-97 14.902893977845219 23.174414314007517 42.046644024910954 19.876047683236315 98-99 14.44858616664784 23.55359765051395 41.50909296283746 20.488723220000754 100-101 13.910162280206334 23.252381490266952 42.071614142098724 20.76584208742799 102-103 14.42911577330301 23.205867734988026 41.48756720936187 20.877449282347094 104-105 13.919198765013743 23.94442561843443 40.93527617756693 21.2010994389849 106-107 14.158772233685257 23.52064099281594 40.76916125728572 21.551425516213083 108-109 14.065394520836785 23.82110637528804 39.68025663810112 22.433242465774057 110-111 14.045709552317481 23.732821265860913 39.31322715463685 22.90824202718476 112-113 13.952717714664436 23.293190838774787 40.06266145492066 22.69142999164012 114-115 14.499630918485712 23.905183712206806 38.542655277865656 23.052530091441827 116-117 13.915879543845378 24.497973817809314 38.48164384387099 23.10450279447432 118-119 13.625546015966261 23.417683386052115 38.65115228197018 24.305618316011447 120-121 13.762227861827986 23.903728415329354 37.880579256124285 24.453464466718376 122-123 13.430228179832818 24.235258679117404 37.250545974847505 25.083967166202275 124-125 13.383537917011823 23.999548158746894 36.910158897507344 25.70675502673394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 496.0 1 338.0 2 372.5 3 566.0 4 410.0 5 372.0 6 574.5 7 1556.0 8 2435.5 9 2548.5 10 1882.5 11 914.5 12 680.0 13 608.5 14 565.0 15 510.0 16 470.5 17 433.0 18 418.0 19 419.0 20 410.5 21 416.0 22 440.0 23 437.0 24 431.0 25 438.0 26 544.5 27 687.0 28 743.5 29 902.0 30 1182.5 31 1348.5 32 1605.0 33 1977.5 34 2152.0 35 2387.5 36 2919.0 37 3370.0 38 3193.5 39 2774.5 40 2550.0 41 2241.5 42 1790.5 43 1718.0 44 1813.0 45 1663.0 46 1326.0 47 1047.5 48 1151.5 49 1109.5 50 970.5 51 764.5 52 447.5 53 344.5 54 313.5 55 306.5 56 289.0 57 248.0 58 226.5 59 198.0 60 177.0 61 166.5 62 130.0 63 103.0 64 76.0 65 58.5 66 57.0 67 51.0 68 46.0 69 45.5 70 50.0 71 38.5 72 42.0 73 45.0 74 32.5 75 24.0 76 18.5 77 17.0 78 12.0 79 3.5 80 0.0 81 0.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06927919515648061 2 0.0 3 0.0 4 0.0 5 0.057230639477092685 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 7.530347299617459E-4 44-45 0.007530347299617458 46-47 0.011295520949426189 48-49 0.006024277839693967 50-51 0.005271243109732221 52-53 0.0030121389198469834 54-55 0.004518208379770476 56-57 0.004518208379770476 58-59 0.0015060694599234917 60-61 7.530347299617459E-4 62-63 7.530347299617459E-4 64-65 0.0015060694599234917 66-67 0.0015060694599234917 68-69 0.0 70-71 0.002259104189885238 72-73 7.530347299617459E-4 74-75 7.530347299617459E-4 76-77 7.530347299617459E-4 78-79 0.0 80-81 7.530347299617459E-4 82-83 7.530347299617459E-4 84-85 0.0 86-87 7.530347299617459E-4 88-89 0.0015060694599234917 90-91 0.002259104189885238 92-93 0.0 94-95 0.002259104189885238 96-97 0.002259104189885238 98-99 7.530347299617459E-4 100-101 7.530347299617459E-4 102-103 0.0015060694599234917 104-105 7.530347299617459E-4 106-107 0.0015060694599234917 108-109 0.0015060694599234917 110-111 7.530347299617459E-4 112-113 0.014307659869273171 114-115 0.02560318081869936 116-117 0.02560318081869936 118-119 0.012048555679387934 120-121 0.003765173649808729 122-123 0.004518208379770476 124-125 0.004518208379770476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 66398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.133407632760022 #Duplication Level Percentage of deduplicated Percentage of total 1 66.2944582299421 19.313834754058856 2 10.153019023986767 5.915840838579475 3 6.415425971877585 5.60709659929516 4 4.29590570719603 5.006174884785686 5 2.8691066997518613 4.179342751287689 6 2.0626550868486353 3.605530287056839 7 1.4164598842018197 2.888641224133257 8 1.0752688172043012 2.5060995813126903 9 0.8529776674937966 2.236513147986385 >10 4.280397022332506 22.66182716346878 >50 0.20161290322580644 3.8465014006445974 >100 0.0413564929693962 1.899153588963523 >500 0.025847808105872623 5.763727823127203 >1k 0.015508684863523574 14.56971595529986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3412 5.138708997258953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3408 5.1326847194192595 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2854 4.298322238621645 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 945 1.4232356396276997 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 883 1.329859333112443 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 1.0949124973643785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 1.0376818578872857 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.8780384951353957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.5948974366697792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 143 0.2153679327690593 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.20633151600951838 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.1972950992499774 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 130 0.19578902979005391 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 112 0.16867977951143107 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 108 0.1626555016717371 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 105 0.15813729329196663 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 95 0.1430765986927317 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 93 0.14006445977288473 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91 0.13705232085303776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 91 0.13705232085303776 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 87 0.13102804301334378 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 86 0.12952197355342027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 80 0.12048555679387933 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.12048555679387933 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 79 0.11897948733395583 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 75 0.11295520949426187 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 74 0.1114491400343384 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 69 0.10391879273472092 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 68 0.10241272327479745 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 67 0.10090665381487395 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0015060694599234917 0.0 22-23 0.0 0.0 0.0 0.0022591041898852374 0.0 24-25 0.0 0.0 0.0 0.003765173649808729 0.0 26-27 0.0 0.0 0.0 0.009789451489502696 0.0 28-29 0.0 0.0 0.0 0.019578902979005393 0.0 30-31 0.0 0.0 0.0 0.04970029217747523 0.0 32-33 0.0 0.0 0.0 0.10467182746468268 0.0 34-35 0.0 0.0 0.0 0.13705232085303776 0.0 36-37 0.0 0.0 0.0 0.17846923100093376 0.0 38-39 0.0 0.0 0.0 0.24850146088737612 0.0 40-41 0.0 0.0 0.0 0.30723816982439234 0.0 42-43 0.0 0.0 0.0 0.3712461218711407 0.0 44-45 0.0 0.0 0.0 0.4322419349980421 0.0 46-47 0.0 0.0 0.0 0.5158287900237959 0.0 48-49 0.0 0.0 0.0 0.6076990270791289 0.0 50-51 0.0 0.0 0.0 0.6890267779149974 0.0 52-53 0.0 0.0 0.0 0.7921925359197566 0.0 54-55 0.0 0.0 0.0 0.8968643633844393 0.0 56-57 0.0 0.0 0.0 1.001536190849122 0.0 58-59 0.0 0.0 0.0 1.1355763727823127 0.0 60-61 0.0 0.0 0.0 1.2726286936353506 0.0 62-63 0.0 0.0 0.0 1.399138528268924 0.0 64-65 0.0 0.0 0.0 1.5248953281725353 0.0 66-67 0.0 0.0 0.0 1.680020482544655 0.0 68-69 0.0 0.0 0.0 1.8690321997650532 0.0 70-71 0.0 0.0 0.0 2.0670803337449923 0.0 72-73 0.0 0.0 0.0 2.259857224615199 0.0 74-75 0.0 0.0 0.0 2.466188740624718 0.0 76-77 0.0 0.0 0.0 2.669508117714389 0.0 78-79 0.0 0.0 0.0 2.9044549534624537 0.0 80-81 0.0 0.0 0.0 3.146932136510136 0.0 82-83 0.0 0.0 0.0 3.3735955902286214 0.0 84-85 0.0 0.0 0.0 3.706436940871713 0.0 86-87 0.0 0.0 0.0 4.008403867586373 0.0 88-89 0.0 0.0 0.0 4.3224193499804215 0.0 90-91 0.0 0.0 0.0 4.682369950902135 0.0 92-93 0.0 0.0 0.0 4.980571703966987 0.0 94-95 0.0 0.0 0.0 5.3224494713696195 0.0 96-97 0.0 0.0 0.0 5.7072502183800715 0.0 98-99 0.0 0.0 0.0 6.143257327027923 0.0 100-101 0.0 0.0 0.0 6.561191602156692 0.0 102-103 0.0 0.0 0.0 7.043133829332209 0.0 104-105 0.0 0.0 0.0 7.528088195427573 0.0 106-107 0.0 0.0 0.0 7.9595770956956535 0.0 108-109 0.0 0.0 0.0 8.382782613934154 0.0 110-111 0.0 0.0 0.0 8.931744932076267 0.0 112-113 0.0 0.0 0.0 9.54471520226513 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 805 0.0 90.98103 1 CTGTCAG 25 0.0020483406 71.39955 5 CCAATAC 50 8.801362E-8 71.39955 3 CAATACT 60 3.8908183E-9 69.41622 4 ATGGGAG 35 9.089438E-5 67.99957 5 ATATAGG 35 9.089438E-5 67.99957 2 GCTTACT 35 9.089438E-5 67.99957 8 CTTACTA 35 9.089438E-5 67.99957 9 GTTATCA 50 7.3424944E-6 59.5445 1 ATACTTA 50 7.3754436E-6 59.49963 6 CATGGGG 80 5.6024874E-10 59.499622 4 CTCTGTG 15 0.004228417 59.499622 80-81 AGGCTTA 40 1.7598324E-4 59.499622 6 GTATCAA 1360 0.0 54.249657 2 TACTTAT 55 1.297284E-5 54.09057 7 TCATTAA 35 0.0077643013 50.999676 8 CCCTTGA 35 0.0077643013 50.999676 9 CTTCTGT 35 0.0077643013 50.999676 2 TTAGCCT 30 2.1828972E-5 49.58302 28-29 TGGGAGG 50 5.296295E-4 47.599705 6 >>END_MODULE