FastQCFastQC Report
Thu 26 May 2016
SRR936273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204708
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53080.1656313149279123No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38870.1212903016437067No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35930.11211629889525036No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35880.11196027844034465No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34420.10740448115709761No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.062.546461
GTACATG149300.048.2161521
GTATCAA42750.046.759532
TACATGG154800.045.6191022
ACATGGG154100.045.247933
CATGGGG94150.039.6837844
ATCAACG53100.037.9820984
TCAACGC53350.037.6925965
CAACGCA55500.036.1252336
AACGCAG57350.035.167387
CATGGGA61500.033.6649444
TATCAAC62350.032.5380633
ATGGGGA53500.029.1354185
ACGCAGA69850.028.4485788
ATGGGGG47450.027.8349515
CGCAGAG71650.026.9035369
GAGTACA98450.026.5891171
TATTCCG4950.026.4418135
AGTACAT109900.022.5738352
CCGTTGC5600.022.3106279
Produced by FastQC (version 0.11.5)