FastQCFastQC Report
Thu 26 May 2016
SRR936273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204708
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86490.26988418289591437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.19305971090033788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.18594517815663705No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG57110.17820656359331333No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35550.11093054343796688No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG32980.10291109205581288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.080.820051
GTATCAA95400.054.914872
GTACATG136650.043.16321
TACATGG141750.041.0324252
ACATGGG141600.040.7815933
ATCAACG135200.039.5857354
TCAACGC136050.039.3384135
CAACGCA137250.038.969536
AACGCAG139950.038.21777
TATCAAC141050.038.1127553
CATGGGG89400.032.696384
ACGCAGA163550.032.5210658
CGCAGAG166250.031.8139729
CATGGGA59200.031.0736664
CAGAGTA184000.027.0312589
GTGGTAT19500.025.9700261
GCAGAGT191100.025.9646918
GAGTACA97300.025.1661641
AGAGTAC179300.024.35530510-11
ATGGGGA52350.023.881325
Produced by FastQC (version 0.11.5)