FastQCFastQC Report
Thu 26 May 2016
SRR936272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148041
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.068.296231
GTACATG100300.055.5194471
TACATGG102550.053.315082
ACATGGG104400.050.489783
GTATCAA26550.050.194222
CATGGGA73650.047.9018364
ATGGGAG26800.045.9521455
ATGGGCG1950.042.7133525
TCAACGC30950.041.3283465
CAACGCA31400.041.1156
CATGGGG29250.041.0861784
ATCAACG31800.040.4107484
AACGCAG32600.039.784067
ATGGGAT29900.038.999155
TATCAAC41450.032.2944763
ACGCAGA40750.031.3892618
ATGGGAA27050.031.0114525
ATGGGTA4800.030.986255
CGCAGAG41400.030.7527319
ATGGGGG16150.029.4705145
Produced by FastQC (version 0.11.5)