##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148041 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.45420938799224 31.0 30.0 33.0 25.0 34.0 2 29.559114177977964 31.0 30.0 34.0 25.0 34.0 3 29.601546460448713 31.0 30.0 34.0 25.0 34.0 4 32.95772537740377 35.0 35.0 37.0 25.0 37.0 5 33.26535027930187 35.0 35.0 37.0 27.0 37.0 6 33.37774173570456 37.0 35.0 37.0 27.0 37.0 7 33.35835392638416 37.0 35.0 37.0 27.0 37.0 8 33.27619135553521 37.0 35.0 37.0 26.0 37.0 9 34.56368892748604 39.0 35.0 39.0 25.0 39.0 10-11 34.45090288587254 39.0 35.0 39.0 25.0 39.0 12-13 34.45787084259186 39.0 35.0 39.0 25.5 39.0 14-15 35.60062445504995 40.0 36.0 41.0 25.5 41.0 16-17 35.64621690340328 40.0 36.0 41.0 26.0 41.0 18-19 35.7097769156328 40.0 36.0 41.0 26.0 41.0 20-21 35.85420424880296 40.0 36.0 41.0 27.0 41.0 22-23 35.79235323477123 39.5 35.5 41.0 27.0 41.0 24-25 35.8096966920171 39.0 35.0 41.0 27.0 41.0 26-27 35.93788592916107 39.0 35.0 41.0 27.5 41.0 28-29 35.83414486068006 39.0 35.0 41.0 27.0 41.0 30-31 35.76143317181181 39.0 35.0 41.0 27.0 41.0 32-33 35.59143967854807 39.0 34.5 41.0 27.0 41.0 34-35 35.54832231601485 39.0 35.0 41.0 27.0 41.0 36-37 35.44651541190602 39.0 34.0 41.0 27.0 41.0 38-39 35.19526959403018 39.0 34.0 41.0 26.0 41.0 40-41 35.298739766262706 39.0 34.5 41.0 26.5 41.0 42-43 35.22716174770761 39.0 34.0 41.0 26.0 41.0 44-45 35.135790882032964 39.0 34.0 41.0 26.0 41.0 46-47 35.11453554359122 39.0 34.0 41.0 26.0 41.0 48-49 35.01565884842092 39.0 34.0 41.0 26.0 41.0 50-51 34.88648271272542 39.0 33.5 40.0 26.0 41.0 52-53 34.73608303187778 38.0 33.0 40.0 26.0 41.0 54-55 34.56322291625473 38.0 33.0 40.0 26.0 41.0 56-57 34.37175458019357 38.0 33.0 40.0 25.0 41.0 58-59 34.14867892348792 38.0 33.0 40.0 24.5 41.0 60-61 33.90593759282116 37.5 32.5 40.0 23.5 41.0 62-63 33.64930956298599 37.0 32.0 40.0 23.0 41.0 64-65 33.359087349667824 36.5 32.0 40.0 22.5 41.0 66-67 33.06703419128759 36.0 32.0 39.0 22.0 41.0 68-69 32.765926042711015 36.0 31.0 39.0 22.0 41.0 70-71 32.42022105482295 35.0 31.0 39.0 20.5 40.5 72-73 32.08019225794201 35.0 31.0 38.0 20.0 40.0 74-75 31.729109848864283 35.0 31.0 37.0 19.0 39.5 76-77 31.395687958879517 35.0 31.0 37.0 18.0 39.0 78-79 31.048413776163045 35.0 30.5 36.5 16.5 39.0 80-81 30.726783276903873 35.0 30.0 36.0 14.0 38.5 82-83 30.427771743343662 34.0 30.0 35.5 11.5 37.0 84-85 30.449686901425995 34.5 30.5 35.0 11.5 37.0 86-87 30.323647413289248 35.0 31.0 35.0 9.0 36.5 88-89 30.144125950205613 34.0 30.0 35.0 7.0 36.0 90-91 29.983288924350262 34.0 30.0 35.0 7.0 36.0 92-93 29.86498609370223 34.0 30.0 35.0 3.5 36.0 94-95 29.74225484978324 34.0 30.0 35.0 2.0 35.0 96-97 29.638394012060544 34.0 30.0 35.0 2.0 35.0 98-99 29.531781094926053 34.0 30.0 35.0 2.0 35.0 100-101 29.43155296718497 34.0 30.0 35.0 2.0 35.0 102-103 28.46806516492007 33.5 28.0 34.5 2.0 35.0 104-105 29.135584443412732 34.0 29.5 35.0 2.0 35.0 106-107 29.262695757381486 34.0 30.0 35.0 2.0 35.0 108-109 29.218897670030948 34.0 30.0 35.0 2.0 35.0 110-111 29.114151846493286 34.0 30.0 35.0 2.0 35.0 112-113 29.084336273704512 34.0 30.0 35.0 2.0 35.0 114-115 28.98736064304324 34.0 29.5 35.0 2.0 35.0 116-117 28.876506152654827 34.0 29.0 35.0 2.0 35.0 118-119 28.68079275914362 34.0 29.0 35.0 2.0 35.0 120-121 28.494538958103412 34.0 29.0 35.0 2.0 35.0 122-123 28.229578908767195 34.0 29.0 35.0 2.0 35.0 124-125 26.250499328856723 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4212946009574061 1101 2 0.5115715087294603 1101 3 0.38131256573871397 1101 4 0.5074428252726477 1101 5 0.3707186217803411 1101 6 0.36735365295727007 1101 7 0.35350525512345143 1101 8 0.43949789102713055 1101 9 0.5534168235097923 1101 10-11 0.5945031371715146 1101 12-13 0.4188035236485561 1101 14-15 0.5962801020320683 1101 16-17 0.6140924996070964 1101 18-19 0.511293993577091 1101 20-21 0.4568677269432655 1101 22-23 0.6287600666100914 1101 24-25 0.680025924552055 1101 26-27 0.44216757308777943 1101 28-29 0.5820301830678289 1101 30-31 0.5942793246529945 1101 32-33 0.7776076647424688 1101 34-35 0.6950699730426138 1101 36-37 0.5468291431057182 1101 38-39 0.6710529924372963 1101 40-41 0.6457972460916253 1101 42-43 0.7369147674411849 1101 44-45 0.6148516898772556 1101 46-47 0.5645012314179354 1101 48-49 0.6265942783420471 1101 50-51 0.5980741218047712 1101 52-53 0.5217939288763915 1101 54-55 0.6020420458047013 1101 56-57 0.5912444412725719 1101 58-59 0.46740036799662477 1101 60-61 0.5187217254654826 1101 62-63 0.6257481218025944 1101 64-65 0.8009691189008237 1101 66-67 0.8172745457484112 1101 68-69 0.7485462451601279 1101 70-71 0.6824916085998751 1101 72-73 0.6022516229413384 1101 74-75 0.5412607551910895 1101 76-77 0.5985954342691002 1101 78-79 0.44074862359642 1101 80-81 0.48910649684145824 1101 82-83 0.45745369004804814 1101 84-85 0.2795264775193296 1101 86-87 0.41828355912495496 1101 88-89 0.4238728578725457 1101 90-91 0.2922701007900059 1101 92-93 0.23128965966530046 1101 94-95 0.268379259637733 1101 96-97 0.2719740504662802 1101 98-99 0.22533938345955917 1101 100-101 0.19963613948736025 1101 102-103 0.24191033532931172 1101 104-105 0.2847233148725081 1101 106-107 0.22616753534639855 1101 108-109 0.34857622494437734 1101 110-111 0.37652062765284455 1101 112-113 0.231374212364436 1101 114-115 0.3101215189681845 1101 116-117 0.3911553669280998 1101 118-119 0.4516187207347073 1101 120-121 0.4624383275285382 1101 122-123 0.38729052673037856 1101 124-125 0.372480568314554 1102 1 0.44941118618686104 1102 2 0.41709178972294936 1102 3 0.40550305603455783 1102 4 0.6230442966325853 1102 5 0.44712808986150776 1102 6 0.5080904309547947 1102 7 0.498953347041315 1102 8 0.5810613833236857 1102 9 0.6975721768519492 1102 10-11 0.8429646186690789 1102 12-13 0.539907273420333 1102 14-15 0.8345407589858524 1102 16-17 0.7023823947963166 1102 18-19 0.6671207014922231 1102 20-21 0.5697624271619759 1102 22-23 0.6484757662657472 1102 24-25 0.8110869533038425 1102 26-27 0.5258364864596174 1102 28-29 0.5952927232704042 1102 30-31 0.6153752222348956 1102 32-33 0.7227738438924192 1102 34-35 0.686740587083861 1102 36-37 0.6932935509803784 1102 38-39 0.6021765195133071 1102 40-41 0.5516788554968883 1102 42-43 0.8132996575296758 1102 44-45 0.7006751675419309 1102 46-47 0.694316443216394 1102 48-49 0.6779128078503263 1102 50-51 0.6027851165303986 1102 52-53 0.6532028798621212 1102 54-55 0.5947002482788406 1102 56-57 0.6245600699393208 1102 58-59 0.648573689447403 1102 60-61 0.7339448234161239 1102 62-63 0.6474476165812035 1102 64-65 0.6676957516136852 1102 66-67 0.62458212560135 1102 68-69 0.7275617465320323 1102 70-71 0.5539882870597239 1102 72-73 0.5170978157783566 1102 74-75 0.5665199093761757 1102 76-77 0.5692601636366703 1102 78-79 0.5030308530054874 1102 80-81 0.48185120108202284 1102 82-83 0.6162192236586144 1102 84-85 0.5018452738743804 1102 86-87 0.4731036546024754 1102 88-89 0.569711963310219 1102 90-91 0.4633610189344388 1102 92-93 0.6893152360990271 1102 94-95 0.6264624187971535 1102 96-97 0.5572540033786737 1102 98-99 0.5642657529006279 1102 100-101 0.644512809035291 1102 102-103 0.6393760570094003 1102 104-105 0.6183484985690662 1102 106-107 0.5575452690894913 1102 108-109 0.6920386704099641 1102 110-111 0.7851939139922024 1102 112-113 0.7427854232487192 1102 114-115 0.694802854121356 1102 116-117 0.5643673339208171 1102 118-119 0.5416858920694878 1102 120-121 0.697003028934656 1102 122-123 0.6671416160142449 1102 124-125 0.5922339967849375 1103 1 0.14061812767847925 1103 2 0.1861116463332877 1103 3 0.10425852421781556 1103 4 0.06943678736062253 1103 5 0.16138446404383444 1103 6 0.215288491457315 1103 7 0.27848593317403214 1103 8 0.33907138856812225 1103 9 0.4177076527605408 1103 10-11 0.5295205032664683 1103 12-13 0.3319165815008418 1103 14-15 0.26975209072596584 1103 16-17 0.2385466742522766 1103 18-19 0.27035811721232506 1103 20-21 0.18646248651511854 1103 22-23 0.26760365188061996 1103 24-25 0.35969623688939834 1103 26-27 0.19390370939191115 1103 28-29 0.2786391415122367 1103 30-31 0.2284575660874495 1103 32-33 0.259851345282037 1103 34-35 0.3790523135694954 1103 36-37 0.27879348346225186 1103 38-39 0.3660098847738169 1103 40-41 0.1726068058035466 1103 42-43 0.3606053701913723 1103 44-45 0.4378861349981946 1103 46-47 0.4053419964724938 1103 48-49 0.2804684474982224 1103 50-51 0.08413692366093528 1103 52-53 0.2100226818298836 1103 54-55 0.3655374416601802 1103 56-57 0.2758513933888338 1103 58-59 0.21476406544486792 1103 60-61 0.141344810226542 1103 62-63 0.28837710567310637 1103 64-65 0.24265563328423667 1103 66-67 0.2843529408120844 1103 68-69 0.33443980251240646 1103 70-71 0.32928952782307874 1103 72-73 0.2606553302319483 1103 74-75 0.31956733222494194 1103 76-77 0.3107693665145135 1103 78-79 0.17638034881779419 1103 80-81 0.15747307444313918 1103 82-83 0.09887789910996148 1103 84-85 0.21510536312392503 1103 86-87 0.27864390953836704 1103 88-89 0.341236271406423 1103 90-91 0.31204331630939564 1103 92-93 0.3048331001395006 1103 94-95 0.4675746778909051 1103 96-97 0.4937715753639935 1103 98-99 0.37094565102676214 1103 100-101 0.3870055783148558 1103 102-103 0.3787502738776247 1103 104-105 0.38793927590684163 1103 106-107 0.34065631599731816 1103 108-109 0.21931222560053598 1103 110-111 0.27927869791849247 1103 112-113 0.396002426511572 1103 114-115 0.44447499865411544 1103 116-117 0.3936318887879331 1103 118-119 0.32269084444565976 1103 120-121 0.15264832951593732 1103 122-123 0.2127584333667194 1103 124-125 0.38546349875238306 1104 1 -0.03009984044629377 1104 2 0.07043769431363245 1104 3 0.06153374429834102 1104 4 -0.05030283830086546 1104 5 0.09040903737609085 1104 6 -0.10633709557917825 1104 7 -0.08101956030885304 1104 8 0.001859323378376132 1104 9 0.03128068103134751 1104 10-11 0.20016293987893619 1104 12-13 0.09343754110776814 1104 14-15 0.04903846527541589 1104 16-17 0.010027091893462625 1104 18-19 -0.14919477345775078 1104 20-21 -0.09336501626109595 1104 22-23 -0.05538041670882876 1104 24-25 0.05864980749817761 1104 26-27 -0.08623863726516134 1104 28-29 0.007965065416172479 1104 30-31 0.02772334395346121 1104 32-33 0.11124798032989958 1104 34-35 -0.023213011417631435 1104 36-37 -0.03565723028727774 1104 38-39 0.09312941998359747 1104 40-41 0.011959499192130352 1104 42-43 0.18514721806128875 1104 44-45 0.15247178183951604 1104 46-47 0.15865251008678172 1104 48-49 -0.0390396205700938 1104 50-51 -0.13830400046013835 1104 52-53 -0.14269769337716554 1104 54-55 0.026754569167302122 1104 56-57 -0.10592581501609999 1104 58-59 -0.011786424346752256 1104 60-61 0.07002832853711993 1104 62-63 -0.025918977855127423 1104 64-65 -0.11264506588630496 1104 66-67 -0.08891216083750919 1104 68-69 -0.03672623653443452 1104 70-71 0.009263130567653377 1104 72-73 -0.018494870461644553 1104 74-75 -0.01843494457711614 1104 76-77 -0.003939581255522739 1104 78-79 -0.07319484458630399 1104 80-81 -0.13840789906034345 1104 82-83 0.009200745919027753 1104 84-85 -0.04781161114883048 1104 86-87 -0.09532129323349992 1104 88-89 -0.12007499907393537 1104 90-91 -0.13635888976910948 1104 92-93 -0.06721248553215631 1104 94-95 -0.08851204531324797 1104 96-97 -0.1079257898735193 1104 98-99 -0.09310010835186588 1104 100-101 -0.0847920096398056 1104 102-103 0.08566908448119648 1104 104-105 0.027382855263009986 1104 106-107 -0.014093949216000823 1104 108-109 -0.148704444064645 1104 110-111 -0.0747883715829083 1104 112-113 -0.0580672796134607 1104 114-115 -0.056892633533788484 1104 116-117 -0.13798293462032518 1104 118-119 -0.03283458811571549 1104 120-121 -0.041491696912334675 1104 122-123 -0.05781839784594567 1104 124-125 0.03682337616489306 1105 1 0.2767029311448965 1105 2 0.26013826347968205 1105 3 0.34055378884177046 1105 4 0.333735766005006 1105 5 0.2534070576680989 1105 6 0.2124008405752349 1105 7 0.18828630457607431 1105 8 0.10175786334372816 1105 9 0.11319976046713265 1105 10-11 0.17778110369977185 1105 12-13 0.1166155910953961 1105 14-15 0.1033585593115447 1105 16-17 0.010432313373300417 1105 18-19 0.09392202931384475 1105 20-21 0.09071194488713985 1105 22-23 0.03293868747330464 1105 24-25 0.3100312724820995 1105 26-27 0.2463046688471806 1105 28-29 0.3410540285441144 1105 30-31 0.32896128010787606 1105 32-33 0.5351523356874779 1105 34-35 0.4752508589114939 1105 36-37 0.27781158931183114 1105 38-39 0.2924173437648392 1105 40-41 0.22837746504217193 1105 42-43 0.43915303345351475 1105 44-45 0.49843008423991364 1105 46-47 0.28185778105930837 1105 48-49 0.4056448634685097 1105 50-51 0.358848313323584 1105 52-53 0.38117325440803995 1105 54-55 0.2781651111123651 1105 56-57 0.27981843145754226 1105 58-59 0.37230662192892794 1105 60-61 0.26277702935374236 1105 62-63 0.30566664451556846 1105 64-65 0.328711034088947 1105 66-67 0.26189704480465537 1105 68-69 0.28467811882315175 1105 70-71 0.15771339040432508 1105 72-73 0.3046726623272704 1105 74-75 0.23331152906802544 1105 76-77 0.1590547270839977 1105 78-79 0.3034996617240182 1105 80-81 0.3171891066313144 1105 82-83 0.33898854596852246 1105 84-85 0.31121956925206007 1105 86-87 0.36908417570982976 1105 88-89 0.3689722664543993 1105 90-91 0.41918119931775166 1105 92-93 0.46901678939979163 1105 94-95 0.3827642476835358 1105 96-97 0.3675258310123972 1105 98-99 0.3578173625711756 1105 100-101 0.27464872285215236 1105 102-103 0.3463230067776557 1105 104-105 0.40749668847261233 1105 106-107 0.43667999287754 1105 108-109 0.31526239582647264 1105 110-111 0.4064885400726226 1105 112-113 0.30236578151002647 1105 114-115 0.3195964780722491 1105 116-117 0.3406312697845273 1105 118-119 0.4090139642228152 1105 120-121 0.37827197544599045 1105 122-123 0.38704735320577655 1105 124-125 0.6269699982881285 1106 1 -0.07766805140790467 1106 2 -0.21774970713556385 1106 3 -0.16222858030423382 1106 4 0.17960353460004086 1106 5 0.01348511092796656 1106 6 -0.029224503777378175 1106 7 -0.12120405355064179 1106 8 -0.02492716879584833 1106 9 -0.05912806322432829 1106 10-11 -0.050382349009140626 1106 12-13 -0.009773297793493896 1106 14-15 -0.19968781350755904 1106 16-17 0.07770733633013549 1106 18-19 -0.047184490429032167 1106 20-21 -0.010729744736373448 1106 22-23 -0.11811962290317979 1106 24-25 -3.9326386081423337 1106 26-27 -0.9147536415293089 1106 28-29 -0.4379519402345906 1106 30-31 -0.42656902292242904 1106 32-33 -5.177797618398834 1106 34-35 -1.044016817856182 1106 36-37 -0.33116223988927374 1106 38-39 0.04156564495859527 1106 40-41 -0.35243520713413545 1106 42-43 0.04134541178225959 1106 44-45 -0.024143377651732578 1106 46-47 -0.04732265971203731 1106 48-49 0.014501612779802997 1106 50-51 -0.0015809796057055792 1106 52-53 0.1382783967404464 1106 54-55 -0.20971367676642672 1106 56-57 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34-35 -1.0873414378581217 2216 36-37 -1.1919408068542978 2216 38-39 -1.038806475901474 2216 40-41 -1.148677913019597 2216 42-43 -0.9707023091094911 2216 44-45 -0.8129907535307694 2216 46-47 -0.9929585128704019 2216 48-49 -1.1449461908445855 2216 50-51 -1.1553858009824154 2216 52-53 -1.4648020739554681 2216 54-55 -1.3744440475670814 2216 56-57 -1.28685622242957 2216 58-59 -1.2007995193561243 2216 60-61 -1.4931095521159037 2216 62-63 -1.7068244807950492 2216 64-65 -1.4799027394289794 2216 66-67 -1.542794116004714 2216 68-69 -1.5058046642794451 2216 70-71 -1.5864160025669207 2216 72-73 -1.628781366781709 2216 74-75 -1.4918402281204735 2216 76-77 -1.4680024259156461 2216 78-79 -1.2347884106934472 2216 80-81 -1.3491216849909762 2216 82-83 -1.2799793276559015 2216 84-85 -1.344270313602042 2216 86-87 -1.2675461047303536 2216 88-89 -1.1551686636130505 2216 90-91 -1.1777969689463603 2216 92-93 -1.150220874781965 2216 94-95 -1.2854794512869994 2216 96-97 -1.436379032317383 2216 98-99 -1.2482626871270845 2216 100-101 -1.2040076491748266 2216 102-103 -1.1574726978726808 2216 104-105 -1.052648856433315 2216 106-107 -1.1502449272502524 2216 108-109 -1.1861770787919177 2216 110-111 -1.209310181972107 2216 112-113 -1.3142014886355184 2216 114-115 -1.2556688751403868 2216 116-117 -1.2076772144185277 2216 118-119 -1.322924267939058 2216 120-121 -1.2065452059526613 2216 122-123 -1.2280547057221618 2216 124-125 -1.5450933669940845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28512.0 3 4571.0 4 3359.0 5 2843.0 6 3231.0 7 3493.0 8 3680.0 9 4066.0 10 4971.0 11 5698.0 12 6189.0 13 6588.0 14 6780.0 15 6239.0 16 5971.0 17 5238.0 18 4567.0 19 4318.0 20 4017.0 21 4031.0 22 4993.0 23 6868.0 24 10669.0 25 16635.0 26 19725.0 27 16766.0 28 18435.0 29 23265.0 30 27020.0 31 33247.0 32 42799.0 33 56855.0 34 78513.0 35 114873.0 36 190631.0 37 271491.0 38 96595.0 39 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.84795812685806 18.320283544374362 9.604203341524112 22.227554987243465 2 19.368488902825685 20.98480889167625 37.04113097104631 22.60557123445176 3 16.973174303008342 28.912904678491447 30.582705669919452 23.531215348580755 4 10.830729618070466 17.06745154128039 36.2893595078216 35.812459332827544 5 11.238865747878547 43.20673429532575 33.90230602926394 11.652093927531764 6 30.709804761879873 37.38843971520007 18.78184792436461 13.119907598555445 7 28.061712457993405 35.57446904882608 21.120628831550285 15.243189661630229 8 27.40625244984887 34.26487112706114 19.484769561771035 18.844106861318956 9 27.73241000849285 14.051349055987455 18.68373946560397 39.53250146991573 10-11 23.75680188706363 28.011274996145563 28.692182326722282 19.539740790068528 12-13 24.349817033282434 27.405069410630272 27.923438816121283 20.32167473996601 14-15 22.515792517848247 28.355888265976315 25.084929425824814 24.043389790350624 16-17 22.436466422477057 30.46889631233041 25.64374158874231 21.450895676450223 18-19 21.687816932785314 29.005575273156154 27.615959889043314 21.69064790501522 20-21 23.282034307267157 28.252167342112312 27.084579151268613 21.381219199351918 22-23 23.032558520722723 28.68303362219701 26.592212233061936 21.692195624018336 24-25 22.424305246749718 28.5882464130493 27.15107069547406 21.836377644726923 26-27 22.216560309819847 28.48508129635146 27.121170151791517 22.177188242037175 28-29 22.184471431831685 28.450531608169154 27.25765792760791 22.107339032391252 30-31 22.27078362866508 28.095365020630975 27.40953859259898 22.224312758104965 32-33 21.83512714019916 28.40681031058584 27.249996515752283 22.508066033462715 34-35 22.414367550019314 28.33410929926574 27.330783171019263 21.920739979695682 36-37 22.553483755511046 27.970432970060592 26.92233820295627 22.553745071472093 38-39 22.107412370806255 28.216946630912776 27.373417239131086 22.302223759149886 40-41 22.265586380837775 28.144877810195645 27.57180891257585 22.01772689639073 42-43 22.138315200822277 28.100006097401636 27.378857695356395 22.382821006419693 44-45 22.01547693398523 28.228418919001314 27.403397643443427 22.352706503570026 46-47 22.345864569963446 28.372957197342043 27.076643399160904 22.204534833533604 48-49 22.214019825990157 28.199707588203704 27.141598474267198 22.444674111538944 50-51 22.125210415255733 28.27824246370405 27.281837760855034 22.314709360185187 52-53 22.426301820427568 28.077911059583094 27.17584168316599 22.31994543682335 54-55 22.06116834207513 28.24873250097885 27.380853349895055 22.309245807050957 56-57 22.264139438864838 28.50787871397089 27.144630366802264 22.083351480362012 58-59 22.429396936338346 27.95177511545785 27.103545353028384 22.51528259517542 60-61 22.23758662620336 28.29885125553554 27.374272235365442 22.089289882895656 62-63 22.005546888542206 28.344182343508145 27.343599606630832 22.306671161318814 64-65 21.812081267663356 28.38562637762748 27.20251630287792 22.599776051831242 66-67 21.935486399828577 28.76688643310277 26.91128421819157 22.386342948877083 68-69 22.126876185988213 28.236460299092236 27.306527949144648 22.3301355657749 70-71 21.861244200434044 28.493948591445324 27.37286249546509 22.271944712655543 72-73 22.214784421616155 28.52171027216398 27.028164100625457 22.235341205594406 74-75 21.880664540726332 28.748058649473318 27.115625661452857 22.25565114834749 76-77 21.813733923149925 28.552085052748577 27.23231453942609 22.40186648467541 78-79 22.12021265800292 28.629869765047488 27.01908164389671 22.230835933052884 80-81 21.97751992745891 28.664705641533082 26.953265524951682 22.40450890605633 82-83 22.133321951621046 28.701382921595535 26.924572782928824 22.240722343854593 84-85 21.82138411674168 28.352782440317792 27.38164818442579 22.444185258514736 86-87 21.864133653879655 28.337078847426916 27.175969478415386 22.62281802027804 88-89 21.978695845698624 28.317548445653852 27.345281824049533 22.358473884597995 90-91 21.7974983450054 28.396745757987528 27.397956517194523 22.407799379812552 92-93 21.87937702519076 28.592731960558865 27.288030033796733 22.239860980453642 94-95 21.706981081209385 28.295740952938424 27.341243346804134 22.656034619048054 96-97 22.11310857606256 28.36115687757858 27.11555405154095 22.4101804948179 98-99 21.621254367237295 28.689667667505493 26.937589990566508 22.75148797469071 100-101 21.458604085586167 28.552588943203872 27.309030378838294 22.679776592371667 102-103 21.808070478626107 28.212878341794163 27.36996052388601 22.609090655693723 104-105 21.542128669253984 28.320179715540405 27.483403184480633 22.654288430724982 106-107 21.55789471850416 28.444391794163337 27.24525744608294 22.752456041249562 108-109 21.519045779467813 28.92338353353748 26.733470582163548 22.82410010483116 110-111 21.56901003104432 28.81751965966224 26.964049907842615 22.649420401450826 112-113 21.7041066131493 28.68301019521558 27.178139060667387 22.434744130967733 114-115 21.318719415210996 29.001696807945383 26.87872701077839 22.800856766065237 116-117 21.394068389000164 28.90080489671269 27.074816066227918 22.63031064805923 118-119 21.591234746736372 28.965935648191298 26.72323008136526 22.71959952370707 120-121 21.446879084419354 28.8261305280799 26.94448303690693 22.782507350593818 122-123 21.54342586519424 28.729953825530014 26.862679512942528 22.863940796333218 124-125 21.82115685043938 28.690998872425556 26.552352946044106 22.935491331090958 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 451.5 2 551.0 3 564.0 4 453.0 5 372.0 6 355.5 7 333.5 8 348.0 9 367.5 10 347.5 11 337.0 12 348.0 13 370.5 14 398.0 15 475.5 16 542.5 17 592.0 18 719.5 19 911.5 20 1183.5 21 1692.0 22 2326.5 23 3231.5 24 4462.0 25 5922.0 26 7904.5 27 9881.0 28 11100.5 29 12737.0 30 15760.5 31 19193.5 32 22480.5 33 25020.0 34 28088.5 35 30388.0 36 32300.0 37 35173.5 38 35777.5 39 34813.0 40 34644.0 41 36138.0 42 37351.5 43 39096.0 44 42172.0 45 43480.0 46 42776.0 47 41612.0 48 41176.0 49 40207.0 50 38664.0 51 36701.0 52 34744.5 53 34089.5 54 33484.0 55 31384.0 56 28280.5 57 25511.0 58 23648.5 59 21751.5 60 18649.5 61 15394.5 62 12659.0 63 9738.0 64 7484.0 65 6226.0 66 4842.0 67 3941.5 68 3171.5 69 2234.0 70 1703.0 71 1334.5 72 1190.0 73 1069.5 74 844.0 75 700.5 76 476.0 77 309.5 78 225.5 79 148.0 80 102.0 81 63.0 82 40.5 83 29.0 84 15.0 85 15.0 86 14.5 87 7.5 88 4.5 89 4.0 90 3.0 91 3.0 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7420980609577532E-4 2 8.710490304788766E-5 3 0.0 4 1.7420980609577532E-4 5 6.097343213352137E-4 6 6.097343213352137E-4 7 6.097343213352137E-4 8 9.581539335267642E-4 9 0.0013936784487662026 10-11 0.0012194686426704273 12-13 0.0015678882548619778 14-15 0.002526042188388742 16-17 0.002264727479245079 18-19 0.0022211750277211354 20-21 0.002918014252104237 22-23 0.09368132322800318 24-25 0.0022211750277211354 26-27 0.0016549931579098655 28-29 0.001306573545718315 30-31 8.710490304788766E-4 32-33 0.0014807833518140903 34-35 8.274965789549328E-4 36-37 4.790769667633821E-4 38-39 6.532867728591575E-4 40-41 0.0010017063850507081 42-43 9.581539335267642E-4 44-45 8.710490304788766E-4 46-47 0.0014372309002901465 48-49 2.1776225761971916E-4 50-51 0.0011759161911464836 52-53 0.0012194686426704273 54-55 0.0010888112880985958 56-57 9.581539335267642E-4 58-59 2.61314709143663E-4 60-61 4.355245152394383E-4 62-63 8.710490304788766E-4 64-65 6.532867728591575E-4 66-67 3.4841961219155064E-4 68-69 4.355245152394383E-5 70-71 2.1776225761971916E-4 72-73 1.306573545718315E-4 74-75 0.0 76-77 4.355245152394383E-5 78-79 4.355245152394383E-5 80-81 1.7420980609577532E-4 82-83 4.355245152394383E-5 84-85 1.7420980609577532E-4 86-87 8.710490304788766E-5 88-89 1.7420980609577532E-4 90-91 8.710490304788766E-5 92-93 8.710490304788766E-5 94-95 2.1776225761971916E-4 96-97 2.1776225761971916E-4 98-99 3.4841961219155064E-4 100-101 4.355245152394383E-4 102-103 4.355245152394383E-4 104-105 3.9197206371549445E-4 106-107 3.9197206371549445E-4 108-109 3.9197206371549445E-4 110-111 4.355245152394383E-4 112-113 2.1776225761971916E-4 114-115 2.61314709143663E-4 116-117 4.790769667633821E-4 118-119 6.968392243831013E-4 120-121 3.9197206371549445E-4 122-123 3.4841961219155064E-4 124-125 1.306573545718315E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1148041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.08628363292341 #Duplication Level Percentage of deduplicated Percentage of total 1 55.03198326077121 18.208038070481695 2 13.888674731747077 9.190492629200005 3 9.623429235997145 9.55210527670707 4 6.519713047417181 8.62852300368465 5 4.38909148156695 7.260936282498606 6 2.8016483622854715 5.561767941257548 7 1.8567448674565823 4.300295112305029 8 1.2151078908201052 3.2162723456221864 9 0.8585688764883643 2.5566168029304968 >10 3.4710235712079345 19.87197327815441 >50 0.23900862196193337 5.51886426319378 >100 0.10237432186522227 5.643364921401426 >500 0.002631730414821438 0.4907500725631767 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.355245152394383E-5 0.0 24-25 0.0 0.0 0.0 1.7420980609577532E-4 0.0 26-27 0.0 0.0 0.0 3.0486716066760683E-4 0.0 28-29 0.0 0.0 0.0 3.4841961219155064E-4 0.0 30-31 0.0 0.0 0.0 0.0016985456094338095 0.0 32-33 0.0 0.0 0.0 0.005923133407256361 0.0 34-35 0.0 0.0 0.0 0.013239945263278926 0.0 36-37 0.0 0.0 0.0 0.024824897368647986 0.0 38-39 0.0 0.0 0.0 0.04512033977880581 0.0 40-41 0.0 0.0 0.0 0.0677676145712566 0.0 42-43 0.0 0.0 0.0 0.0949007918706736 0.0 44-45 0.0 0.0 0.0 0.12678118638620048 0.0 46-47 0.0 0.0 0.0 0.1702900854586204 0.0 48-49 0.0 0.0 0.0 0.21854620174715014 0.0 50-51 0.0 0.0 0.0 0.26667166068110804 0.0 52-53 0.0 0.0 0.0 0.3212428824406097 0.0 54-55 0.0 0.0 0.0 0.3894024690755818 0.0 56-57 0.0 0.0 0.0 0.46901635046135115 0.0 58-59 0.0 0.0 0.0 0.5595618971796303 0.0 60-61 0.0 0.0 0.0 0.6572064934963124 0.0 62-63 0.0 0.0 0.0 0.7562447682617608 0.0 64-65 0.0 0.0 0.0 0.875491380534319 0.0 66-67 0.0 0.0 0.0 1.0203032818514322 0.0 68-69 0.0 0.0 0.0 1.177876051465061 0.0 70-71 0.0 0.0 0.0 1.3502566545968304 0.0 72-73 0.0 0.0 0.0 1.5323059019669159 0.0 74-75 0.0 0.0 0.0 1.731427710334387 0.0 76-77 0.0 0.0 0.0 1.9655221372755851 0.0 78-79 0.0 0.0 0.0 2.232237350408217 0.0 80-81 0.0 0.0 0.0 2.5132377676407027 0.0 82-83 0.0 0.0 0.0 2.8097428576157126 0.0 84-85 0.0 0.0 0.0 3.1435288460952178 0.0 86-87 0.0 0.0 0.0 3.5010944731067966 0.0 88-89 0.0 0.0 0.0 3.8844867038720743 0.0 90-91 0.0 0.0 0.0 4.297581706576681 0.0 92-93 0.0 0.0 0.0 4.730057550209444 0.0 94-95 0.0 0.0 0.0 5.188795522111144 0.0 96-97 0.0 0.0 0.0 5.695397638237658 0.0 98-99 0.0 0.0 0.0 6.249602583879844 0.0 100-101 0.0 0.0 0.0 6.82789203521477 0.0 102-103 0.0 0.0 0.0 7.415588816078868 0.0 104-105 0.0 0.0 0.0 8.023537486901601 0.0 106-107 0.0 0.0 0.0 8.681527924525344 0.0 108-109 0.0 0.0 0.0 9.375057162592626 0.0 110-111 0.0 0.0 0.0 10.118236195397202 0.0 112-113 0.0 0.0 0.0 10.851572374157369 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1960 0.0 68.29623 1 GTACATG 10030 0.0 55.519447 1 TACATGG 10255 0.0 53.31508 2 ACATGGG 10440 0.0 50.48978 3 GTATCAA 2655 0.0 50.19422 2 CATGGGA 7365 0.0 47.901836 4 ATGGGAG 2680 0.0 45.952145 5 ATGGGCG 195 0.0 42.713352 5 TCAACGC 3095 0.0 41.328346 5 CAACGCA 3140 0.0 41.115 6 CATGGGG 2925 0.0 41.086178 4 ATCAACG 3180 0.0 40.410748 4 AACGCAG 3260 0.0 39.78406 7 ATGGGAT 2990 0.0 38.99915 5 TATCAAC 4145 0.0 32.294476 3 ACGCAGA 4075 0.0 31.389261 8 ATGGGAA 2705 0.0 31.011452 5 ATGGGTA 480 0.0 30.98625 5 CGCAGAG 4140 0.0 30.752731 9 ATGGGGG 1615 0.0 29.470514 5 >>END_MODULE