FastQCFastQC Report
Thu 26 May 2016
SRR936272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148041
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64750.5640042472350726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45570.39693704318922407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41330.3600045642969197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.086.39061
GTATCAA60200.058.9468082
GTACATG94150.045.952121
CATGGGA66400.044.3861664
TACATGG96450.044.3233642
ACATGGG98350.043.7092483
ATCAACG85450.042.7128874
TCAACGC85600.042.638045
CAACGCA86350.042.305316
AACGCAG88400.041.3915527
TATCAAC90850.040.3051643
ATGGGAT27550.039.3332945
ATGGGAG23800.039.276545
ACGCAGA101800.035.7093738
CGCAGAG103550.034.990979
CATGGGG28300.033.2415434
ATGGGAC12700.029.5357065
GAGTACA61700.029.2648771
AGTACAT60600.028.6893522
TGGGATA8850.028.2354586
Produced by FastQC (version 0.11.5)