##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148041 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54002513847502 31.0 31.0 34.0 30.0 34.0 2 31.409288518441414 31.0 31.0 34.0 30.0 34.0 3 31.754464344043463 31.0 31.0 34.0 30.0 34.0 4 35.58595206965605 37.0 35.0 37.0 33.0 37.0 5 35.21215705710859 37.0 35.0 37.0 33.0 37.0 6 35.41279623288715 37.0 35.0 37.0 33.0 37.0 7 35.224995448768816 37.0 35.0 37.0 32.0 37.0 8 35.31078942302583 37.0 35.0 37.0 32.0 37.0 9 36.99409602967141 39.0 37.0 39.0 33.0 39.0 10-11 36.87921032436995 39.0 37.0 39.0 32.0 39.0 12-13 36.830053978908424 39.0 37.0 39.0 32.0 39.0 14-15 37.93585072310135 40.0 37.0 41.0 32.5 41.0 16-17 37.850725278975226 40.0 37.0 41.0 32.0 41.0 18-19 37.99700097818806 40.0 37.0 41.0 33.0 41.0 20-21 38.05375766196504 40.0 37.0 41.0 33.0 41.0 22-23 37.949720436813664 40.0 37.0 41.0 32.5 41.0 24-25 37.90461708249096 40.0 37.0 41.0 32.0 41.0 26-27 37.71995817222556 40.0 37.0 41.0 32.0 41.0 28-29 37.55106132969119 40.0 36.5 41.0 32.0 41.0 30-31 37.33323940521288 39.0 36.0 41.0 32.0 41.0 32-33 37.07028233312225 39.0 35.5 41.0 31.0 41.0 34-35 36.84195294418927 39.0 35.0 41.0 30.5 41.0 36-37 36.61085579696196 39.0 35.0 41.0 30.0 41.0 38-39 36.55181957787222 39.0 35.0 41.0 30.0 41.0 40-41 36.54027120982613 39.0 35.0 41.0 30.0 41.0 42-43 36.39201866483863 39.0 35.0 41.0 30.0 41.0 44-45 36.156208271307385 39.0 35.0 41.0 30.0 41.0 46-47 35.76637376191269 39.0 35.0 41.0 27.5 41.0 48-49 35.4059815807972 39.0 35.0 41.0 26.0 41.0 50-51 35.18682477367969 39.0 35.0 41.0 25.5 41.0 52-53 35.033702193562775 39.0 35.0 41.0 24.5 41.0 54-55 34.840776156949104 39.0 34.0 41.0 24.0 41.0 56-57 34.64260292097582 38.0 34.0 40.5 24.0 41.0 58-59 34.429756864084126 38.0 34.0 40.0 23.0 41.0 60-61 34.20341172484258 38.0 34.0 40.0 23.0 41.0 62-63 33.98925081943938 37.0 34.0 40.0 23.0 41.0 64-65 33.74372779369378 37.0 33.0 40.0 22.5 41.0 66-67 33.45841045746624 36.5 33.0 39.5 22.0 41.0 68-69 33.15535812745364 36.0 33.0 39.0 22.0 41.0 70-71 32.82660767341933 35.5 33.0 39.0 21.0 41.0 72-73 32.564089174515544 35.0 32.5 38.5 20.5 40.0 74-75 32.374977461606335 35.0 33.0 37.5 21.0 40.0 76-77 32.077002476392394 35.0 32.0 37.0 20.0 39.0 78-79 31.745235579565538 35.0 32.0 36.5 20.0 39.0 80-81 31.464911531905223 35.0 32.0 36.0 19.5 38.5 82-83 31.21357381835666 35.0 32.0 36.0 19.0 37.0 84-85 30.97144178648672 35.0 32.0 35.0 18.5 37.0 86-87 30.774052930165386 35.0 32.0 35.0 18.0 36.5 88-89 30.62044212706689 35.0 32.0 35.0 18.0 36.0 90-91 30.49411301512751 35.0 32.0 35.0 17.0 36.0 92-93 30.36858221962456 35.0 31.0 35.0 15.0 36.0 94-95 30.25138126600008 34.5 31.0 35.0 12.0 35.5 96-97 30.194349330729477 34.0 31.0 35.0 11.0 35.0 98-99 30.108859352584098 34.0 31.0 35.0 10.0 35.0 100-101 30.03393824785003 34.0 31.0 35.0 7.0 35.0 102-103 29.019376485683004 33.5 29.5 34.5 7.0 35.0 104-105 29.763486234376646 34.0 31.0 35.0 7.0 35.0 106-107 29.91746766883761 34.0 31.0 35.0 6.0 35.0 108-109 29.88487214306806 34.0 31.0 35.0 2.0 35.0 110-111 29.840162502907127 34.0 31.0 35.0 2.0 35.0 112-113 29.772432778968696 34.0 31.0 35.0 2.0 35.0 114-115 29.7528049956404 34.0 31.0 35.0 2.0 35.0 116-117 29.656986989140634 34.0 31.0 35.0 2.0 35.0 118-119 29.52541764623389 34.0 31.0 35.0 2.0 35.0 120-121 29.332316528765087 34.0 31.0 35.0 2.0 35.0 122-123 29.113556920005472 34.0 31.0 35.0 2.0 35.0 124-125 27.05966250334265 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17017864148556328 1101 2 0.5678277516287338 1101 3 0.3189649577020077 1101 4 0.21591064184212883 1101 5 0.49188564511537436 1101 6 0.3141946784760634 1101 7 0.44235072850546686 1101 8 0.42696218623893856 1101 9 0.35884363435754807 1101 10-11 0.36035762691267337 1101 12-13 0.42322234357366284 1101 14-15 0.4286893719343965 1101 16-17 0.47950730526045504 1101 18-19 0.38482603827769424 1101 20-21 0.37546841382273044 1101 22-23 0.3796619314889824 1101 24-25 0.23662747262763162 1101 26-27 0.2460011214000417 1101 28-29 0.3671644253677968 1101 30-31 0.2305902247259155 1101 32-33 0.25681125581812836 1101 34-35 0.15696887159234763 1101 36-37 0.23959639929742593 1101 38-39 0.1294556052858269 1101 40-41 0.035812419205633717 1101 42-43 -0.04969452881213954 1101 44-45 0.04779050178365196 1101 46-47 -0.09771021971193505 1101 48-49 -0.222585825050011 1101 50-51 -0.1582799093916165 1101 52-53 -0.04530860224990363 1101 54-55 -0.09828130852904593 1101 56-57 -0.20255110408120913 1101 58-59 -0.0016670904318800694 1101 60-61 0.009020190110447857 1101 62-63 0.10673229580142163 1101 64-65 0.06384674461236273 1101 66-67 -0.07764414712448087 1101 68-69 -0.14190597161246643 1101 70-71 -0.14103486441530322 1101 72-73 -0.06094759317979026 1101 74-75 -0.006404415490578685 1101 76-77 0.053114694611750224 1101 78-79 0.11906099586815344 1101 80-81 -0.12732033368044782 1101 82-83 -0.11893482522551935 1101 84-85 -0.1320073102045498 1101 86-87 -0.0274414155043452 1101 88-89 -0.04190015987504481 1101 90-91 -0.014567665435762933 1101 92-93 0.05694007144147406 1101 94-95 0.057305506635238146 1101 96-97 -0.11083095578101165 1101 98-99 -0.04908393716384296 1101 100-101 0.11341398277205172 1101 102-103 0.18729429836425027 1101 104-105 0.012604209393806798 1101 106-107 0.2082578047439796 1101 108-109 -0.05101914085486925 1101 110-111 -0.0271534747317439 1101 112-113 0.025238488700761508 1101 114-115 -0.033544453777228256 1101 116-117 -0.01641280600604844 1101 118-119 -0.11793126497394013 1101 120-121 -0.03459345348243659 1101 122-123 -0.01553621634994684 1101 124-125 0.22051920465358776 1102 1 0.1393578386725629 1102 2 0.45187963481368243 1102 3 0.29669474278183117 1102 4 0.09791521015682036 1102 5 0.4397515731229191 1102 6 0.19765605144742437 1102 7 0.35650969513174857 1102 8 0.3425083730750984 1102 9 0.25866948172375004 1102 10-11 0.41485405895420513 1102 12-13 0.41001119371940575 1102 14-15 0.43358358442359446 1102 16-17 0.4574270111927561 1102 18-19 0.3768889426175974 1102 20-21 0.37350600474533735 1102 22-23 0.3454493656869957 1102 24-25 0.354943375801831 1102 26-27 0.3546521279486399 1102 28-29 0.262985336706997 1102 30-31 0.2867110386468852 1102 32-33 0.3561963591286954 1102 34-35 0.2717184122711629 1102 36-37 0.34989062021335826 1102 38-39 0.36029658120450136 1102 40-41 0.18960074970238594 1102 42-43 0.31094793353246075 1102 44-45 0.36455326733769056 1102 46-47 0.3798290061259877 1102 48-49 0.34221165867425185 1102 50-51 0.21474762689763338 1102 52-53 0.3142859438615986 1102 54-55 0.4958268678349711 1102 56-57 0.2527894064515763 1102 58-59 0.3001551283288535 1102 60-61 0.32703643329084997 1102 62-63 0.2023499439492582 1102 64-65 0.2498552275309791 1102 66-67 0.16890025094451744 1102 68-69 0.1705636250913969 1102 70-71 0.27492212601269017 1102 72-73 0.4452032888828654 1102 74-75 0.24503994057239709 1102 76-77 0.2537968775460655 1102 78-79 0.3387062173318256 1102 80-81 0.26436126065882704 1102 82-83 0.3098652835560394 1102 84-85 0.31797104839411006 1102 86-87 0.2558078206627137 1102 88-89 0.24429460507635525 1102 90-91 0.26059604953102067 1102 92-93 0.30499426048901057 1102 94-95 0.25714373875618435 1102 96-97 0.10048647569746194 1102 98-99 0.25985153571035013 1102 100-101 0.26271539322963733 1102 102-103 0.28084803351690724 1102 104-105 0.2825044035918651 1102 106-107 0.1550643255088744 1102 108-109 0.18932617675025298 1102 110-111 0.21415996042895813 1102 112-113 0.19589338687126556 1102 114-115 0.34382859929463905 1102 116-117 0.16119862167528254 1102 118-119 0.2704725188878321 1102 120-121 0.38982634629310553 1102 122-123 0.2897071313131043 1102 124-125 0.3712611408376283 1103 1 0.09743441993141744 1103 2 0.5049400902313046 1103 3 0.27615761252209126 1103 4 0.1123317436191229 1103 5 0.3604727295357293 1103 6 0.20065385632317856 1103 7 0.19380074222799948 1103 8 0.2653458302481866 1103 9 0.16172946608466532 1103 10-11 0.22882026220575113 1103 12-13 0.15078774640082315 1103 14-15 0.19681525325188431 1103 16-17 0.3131115664995221 1103 18-19 0.19921797258622576 1103 20-21 0.21189194464479044 1103 22-23 0.17979426712622626 1103 24-25 -0.04833910434312827 1103 26-27 0.08549692104483597 1103 28-29 0.07189301901379253 1103 30-31 -0.10066385654299381 1103 32-33 0.16001223248352403 1103 34-35 0.0986057009629775 1103 36-37 0.24082874534486365 1103 38-39 0.19358129101787114 1103 40-41 0.11020618318446651 1103 42-43 0.061246426465046966 1103 44-45 0.16218306701297536 1103 46-47 0.1965086530218798 1103 48-49 0.31244699860446445 1103 50-51 0.20166261372374805 1103 52-53 0.1269546999264577 1103 54-55 0.2666552681577983 1103 56-57 0.1793394185420354 1103 58-59 0.2044990562508957 1103 60-61 0.2827432852816898 1103 62-63 0.23505022940962306 1103 64-65 0.14343535270644026 1103 66-67 0.13943267063719844 1103 68-69 0.13684130359833802 1103 70-71 0.3003177351361117 1103 72-73 0.23107457106782192 1103 74-75 0.13473808743532345 1103 76-77 0.20209071015763413 1103 78-79 0.1383763206188604 1103 80-81 0.2283169436439465 1103 82-83 0.19280266755832187 1103 84-85 0.0727689179616462 1103 86-87 0.1080418855994516 1103 88-89 0.12530568118969754 1103 90-91 0.14170479140137004 1103 92-93 -0.008248242643517756 1103 94-95 0.04002679421840227 1103 96-97 0.038328973177307546 1103 98-99 0.1291190111290419 1103 100-101 0.0707232002094429 1103 102-103 0.04993116874472747 1103 104-105 0.10593884663011721 1103 106-107 -0.05087114600318898 1103 108-109 2.207762421377879E-4 1103 110-111 0.009205228978387936 1103 112-113 0.01705686553082586 1103 114-115 0.09287156908734318 1103 116-117 0.19705498706454705 1103 118-119 0.24654095400605058 1103 120-121 0.10633280134016587 1103 122-123 -0.07169573836435106 1103 124-125 -0.03494251895893541 1104 1 0.18226918117739288 1104 2 0.3941947632589553 1104 3 0.26835103523179527 1104 4 0.08410963444428887 1104 5 0.34011954604533656 1104 6 0.2309412172800549 1104 7 0.29546460683582154 1104 8 0.19487477417185772 1104 9 0.27969076665355175 1104 10-11 0.2142899844607129 1104 12-13 0.26272001929446276 1104 14-15 0.192572305444628 1104 16-17 0.26569095988997304 1104 18-19 0.2614677259843674 1104 20-21 0.2668632209567363 1104 22-23 0.13072034240338581 1104 24-25 0.24600750930154902 1104 26-27 0.2008595963803046 1104 28-29 0.18763971082660902 1104 30-31 0.12471256025447275 1104 32-33 0.1264712779637378 1104 34-35 0.17408820308778417 1104 36-37 0.21139890745068612 1104 38-39 0.16838633596454855 1104 40-41 0.1237588939437515 1104 42-43 0.13003764309624444 1104 44-45 0.12442708187733587 1104 46-47 -0.04094256944091512 1104 48-49 0.021673785459817907 1104 50-51 0.010186507737678596 1104 52-53 0.0177218643462993 1104 54-55 0.08117561979033638 1104 56-57 0.053310670038705155 1104 58-59 0.18492662354684342 1104 60-61 0.16191721558797667 1104 62-63 0.1619780665717485 1104 64-65 0.26403963582398404 1104 66-67 0.3239491759011415 1104 68-69 0.3780433558221006 1104 70-71 0.3567540436148775 1104 72-73 0.2765628427455198 1104 74-75 0.2625173989353442 1104 76-77 0.25177183379357615 1104 78-79 0.14791112715272448 1104 80-81 0.11239949167702434 1104 82-83 0.2969714797128482 1104 84-85 0.22892310771169377 1104 86-87 0.19106668226924128 1104 88-89 0.14241814404432418 1104 90-91 0.1491442576246662 1104 92-93 0.16498878671916728 1104 94-95 0.13062056186051763 1104 96-97 0.020663594837326826 1104 98-99 0.009278108100957638 1104 100-101 0.006358099180229004 1104 102-103 0.03542415532586318 1104 104-105 0.039475613571198664 1104 106-107 0.041918929125074555 1104 108-109 -0.028387055460623145 1104 110-111 0.029896985962338363 1104 112-113 0.06896438518142745 1104 114-115 0.1169144747086257 1104 116-117 0.07966738477906077 1104 118-119 0.05375925403520654 1104 120-121 -0.016941606039900137 1104 122-123 0.03269951323158082 1104 124-125 0.13821727284085128 1105 1 0.13066561770300567 1105 2 0.564110625669251 1105 3 0.23998924359606022 1105 4 0.2459792211802494 1105 5 0.3467107363447681 1105 6 0.26786524598581707 1105 7 0.3693238001048158 1105 8 0.2809957838229309 1105 9 0.28300306558946176 1105 10-11 0.15966365434440632 1105 12-13 0.22476097450357457 1105 14-15 0.23481432474677888 1105 16-17 0.366813206858545 1105 18-19 0.210052853428067 1105 20-21 0.24363753424552925 1105 22-23 0.42191435378671116 1105 24-25 0.27901233483978416 1105 26-27 0.2893618235518005 1105 28-29 0.25545151483551365 1105 30-31 0.20760293392435614 1105 32-33 0.17601347049033933 1105 34-35 0.21017951711896643 1105 36-37 0.0978954429617005 1105 38-39 -0.002391318081478744 1105 40-41 0.02476359575023679 1105 42-43 0.05332705165848495 1105 44-45 -0.03089956994153198 1105 46-47 0.06077977651305844 1105 48-49 -0.13568825363941528 1105 50-51 0.05529848497103984 1105 52-53 0.13450422019880648 1105 54-55 0.10770680019123091 1105 56-57 0.08476266211987138 1105 58-59 0.05102103087175891 1105 60-61 -0.024766773523630548 1105 62-63 -0.04422089408156182 1105 64-65 0.00150560959286139 1105 66-67 0.0285761246761993 1105 68-69 0.0021159863590938244 1105 70-71 -0.12062779256428513 1105 72-73 -0.2333783846734363 1105 74-75 -0.1891740140812388 1105 76-77 -0.09061123970308316 1105 78-79 -0.09600003345109087 1105 80-81 -0.06498234450213758 1105 82-83 -0.12306625847076802 1105 84-85 -0.018750735396444895 1105 86-87 -0.006367739903485159 1105 88-89 -0.019322143013337012 1105 90-91 -0.15701387155127833 1105 92-93 -0.11426572948969138 1105 94-95 -0.008566520544832201 1105 96-97 -0.1300794143188213 1105 98-99 -0.15625393842218216 1105 100-101 -0.14622047048074904 1105 102-103 -0.21244270931160258 1105 104-105 -0.24119439385270525 1105 106-107 -0.2598962156410707 1105 108-109 -0.23500051747497608 1105 110-111 -0.1883992278461264 1105 112-113 -0.0639822864345092 1105 114-115 -0.2110699350909293 1105 116-117 -0.20691205100416354 1105 118-119 -0.24439219362626474 1105 120-121 -0.2867250754781594 1105 122-123 -0.24837942762464138 1105 124-125 -0.25023422431331355 1106 1 0.2132826975928097 1106 2 0.49834386717521895 1106 3 0.24287262284307332 1106 4 0.08933551135471873 1106 5 0.36979154442557416 1106 6 0.2490957326708525 1106 7 0.33306576521041364 1106 8 0.28555501247306836 1106 9 0.1414374090238084 1106 10-11 0.16246898033154622 1106 12-13 0.1508468330894317 1106 14-15 0.09659118425091151 1106 16-17 0.10575719216616619 1106 18-19 0.2972957368073139 1106 20-21 0.21964304388740175 1106 22-23 0.1025295971292195 1106 24-25 0.2204930510932286 1106 26-27 0.347936203717083 1106 28-29 0.09730413191540777 1106 30-31 0.16546335633023546 1106 32-33 0.14580226755186487 1106 34-35 0.03377455844128008 1106 36-37 -0.15703339083076884 1106 38-39 -0.30669572579497384 1106 40-41 -0.2912740534692304 1106 42-43 -0.14234558378687723 1106 44-45 -0.07153317875695109 1106 46-47 0.09554561673344608 1106 48-49 0.07301238915213304 1106 50-51 0.00837240599950917 1106 52-53 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-1.328303543062951 2216 58-59 -1.3142034987697002 2216 60-61 -1.031847000715679 2216 62-63 -0.9128311177237265 2216 64-65 -0.821559708121022 2216 66-67 -0.7391581671235841 2216 68-69 -0.7380215713263922 2216 70-71 -0.6760679771578282 2216 72-73 -0.7181556299698499 2216 74-75 -0.7186663818483652 2216 76-77 -0.5881831246871094 2216 78-79 -0.5459503351905255 2216 80-81 -0.4998953725461597 2216 82-83 -0.5245900426383265 2216 84-85 -0.4777674198124906 2216 86-87 -0.4223945414654331 2216 88-89 -0.4572712022962584 2216 90-91 -0.2515399631380717 2216 92-93 -0.23237098330580963 2216 94-95 -0.4881245610134144 2216 96-97 -0.6090845616386673 2216 98-99 -0.697262103136417 2216 100-101 -0.7611601225929228 2216 102-103 -0.6276681264220016 2216 104-105 -0.448662444970914 2216 106-107 -0.37460335089133423 2216 108-109 -0.40926391825594877 2216 110-111 -0.45433107023164254 2216 112-113 -0.39947660663330353 2216 114-115 -0.4755374537277781 2216 116-117 -0.4878412309828626 2216 118-119 -0.4731515120501193 2216 120-121 -0.5245348021377225 2216 122-123 -0.6217535845291486 2216 124-125 -0.8205741249167922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51.0 3 56.0 4 142.0 5 288.0 6 717.0 7 1496.0 8 2390.0 9 3972.0 10 6691.0 11 8420.0 12 9364.0 13 11547.0 14 15967.0 15 11937.0 16 5601.0 17 4757.0 18 4598.0 19 4198.0 20 4243.0 21 4216.0 22 4604.0 23 5433.0 24 6695.0 25 8955.0 26 11236.0 27 14487.0 28 18216.0 29 23199.0 30 28642.0 31 36266.0 32 45547.0 33 58338.0 34 77900.0 35 110284.0 36 183735.0 37 293066.0 38 120367.0 39 420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.67998535641441 18.16920314488433 11.223349545874518 21.927461952826736 2 19.522626546995205 21.36874233470844 36.51048054409633 22.59815057420002 3 17.192984901196652 27.455972711704664 33.0910649299065 22.259977457192186 4 11.323560154698441 16.953067837357583 36.434967422737884 35.28840458520609 5 10.61623536467159 41.72088450032426 35.23566452584674 12.42721560915741 6 30.399262744100604 37.23839131180855 17.968434925233506 14.393911018857342 7 26.066229341983433 35.10127251552863 22.468796846105672 16.36370129638227 8 27.776882532940895 32.706323206226955 20.22114192785798 19.29565233297417 9 26.992938405509907 11.232612772540355 19.998937320182815 41.77551150176692 10-11 24.190120387686502 25.74089252909957 29.845711085231276 20.22327599798265 12-13 25.43820299100816 25.67033755763078 29.572942081336816 19.318517370024242 14-15 22.79587575705049 26.547353273968437 27.935674771197196 22.721096197783876 16-17 21.104690511924225 28.636041744153733 28.66888029260279 21.59038745131925 18-19 20.254111133661603 26.06866827926877 32.51277611165455 21.164444475415074 20-21 21.667039766001388 25.334461051478126 32.733238621268754 20.265260561251733 22-23 21.40145691660838 25.891235591760225 32.366918951500864 20.340388540130537 24-25 20.86005639171423 26.076856140155275 32.616692260990675 20.446395207139815 26-27 20.78092158729523 26.121236088258172 32.423842005642655 20.674000318803945 28-29 20.77739383872179 25.88600929757735 32.60367007798502 20.73292678571584 30-31 20.768204271450237 25.478227693958665 32.8777456554252 20.8758223791659 32-33 20.571956924883345 25.922157832342226 32.54953438074076 20.956350862033673 34-35 21.045617332853094 26.101628036317564 32.14288358545155 20.709871045377795 36-37 21.326111175471958 25.632229162547333 31.776739680899897 21.264919981080816 38-39 21.01196734262975 25.991972412135105 31.88723225041614 21.108827994819002 40-41 21.12664094749229 26.05969647425484 31.821860020678706 20.991802557574164 42-43 21.173033964292262 26.19596703700171 31.282096175614505 21.34890282309152 44-45 21.11658668190573 26.478774347149255 30.926443928925796 21.478195042019223 46-47 21.66685976972243 26.61529254241806 30.051641274340785 21.666206413518722 48-49 21.853717348089017 26.82073658773701 29.451705748199824 21.873840315974153 50-51 21.832515576578402 27.108625537095232 29.237885548898806 21.820973337427564 52-53 21.991007616478285 26.98677623965403 29.259175859331005 21.76304028453668 54-55 21.63051990532763 27.108198115089476 29.316073135016147 21.94520884456675 56-57 21.777426436000628 27.365677102388542 29.21379292322166 21.643103538389173 58-59 21.988827603988824 26.82233050640762 29.099797213956695 22.08904467564686 60-61 21.802513721462798 26.996199540251514 29.604431633971213 21.596855104314475 62-63 21.57823814248271 26.880813778770012 29.661453955351224 21.879494123396054 64-65 21.450802250831867 26.885507221128552 29.618038013275026 22.04565251476455 66-67 21.569336262449763 27.179198163088603 29.31161989313719 21.939845681324442 68-69 21.729158691023247 26.86667076064882 29.33717122383499 22.066999324492944 70-71 21.649434696429434 26.99154146103937 29.412510240533308 21.94651360199789 72-73 21.784751753898473 27.22070462555335 28.850606351490487 22.143937269057695 74-75 21.468736348951186 27.449871933291202 28.97517452654951 22.106217191208106 76-77 21.455530536960982 27.2864503259918 29.203377945794333 22.05464119125288 78-79 21.750486263121267 27.34466800401728 28.774233672839213 22.130612060022248 80-81 21.55399037314096 27.33419973903303 28.675575198730357 22.436234689095656 82-83 21.790685175877865 27.414264821552543 28.575721598793073 22.21932840377652 84-85 21.454130518100413 27.080545974007876 28.858315041287756 22.60700846660395 86-87 21.55551471292762 26.995752753828704 28.76712269106075 22.681609842182922 88-89 21.68713004447197 26.889623192495993 28.758177620464338 22.665069142567702 90-91 21.400809392154503 27.177919935332252 28.809204004801302 22.612066667711943 92-93 21.54488186781599 27.170924953572882 28.665695152416827 22.6184980261943 94-95 21.349124497180377 26.95914552457871 28.629186098572678 23.06254387966823 96-97 21.754008505893804 26.93824843046007 28.47174828129117 22.835994782354952 98-99 21.440536159307747 26.940628927968834 28.30894490238385 23.30989001033957 100-101 21.21630491069737 26.871971182050714 28.56556140081156 23.346162506440354 102-103 21.382809886832423 26.72103216635076 28.4559576736537 23.440200273163118 104-105 21.267749909845875 26.345996721351455 28.88272472766486 23.503528641137805 106-107 21.205283453407862 26.57592917301446 28.571683665247722 23.647103708329958 108-109 21.306386859565393 26.753026273726533 28.05029315461543 23.89029371209264 110-111 21.16162368437786 26.743659361114812 28.23712948517941 23.857587469327918 112-113 21.287473936429933 26.45650002678771 28.426986504656927 23.82903953212543 114-115 20.82841999753444 26.75230209838462 28.23494972824371 24.184328175837237 116-117 20.704839866146376 26.673279195972192 28.36722799112405 24.25465294675738 118-119 20.85633704659545 26.699770235100672 28.014504589853324 24.429388128450558 120-121 20.640660225162286 26.639107922492883 28.15717630743607 24.56305554490876 122-123 20.616845925281954 26.616833729830685 27.920309694773987 24.846010650113374 124-125 20.634077886238632 26.506687387084465 27.87700504014871 24.982229686528186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 58.0 1 54.0 2 205.0 3 402.0 4 353.5 5 360.5 6 587.0 7 1238.5 8 1907.0 9 2356.5 10 2246.5 11 1746.5 12 1718.0 13 1845.5 14 2050.0 15 2296.5 16 2547.0 17 2736.0 18 2921.5 19 3114.0 20 3333.5 21 3717.5 22 4205.0 23 5103.5 24 6398.5 25 7372.0 26 8917.0 27 10943.5 28 12142.5 29 13554.0 30 16313.0 31 19779.5 32 22674.5 33 24833.5 34 27244.5 35 28814.5 36 30671.0 37 33516.0 38 34327.5 39 34054.5 40 33835.5 41 34933.0 42 35908.0 43 37121.5 44 39936.5 45 41817.0 46 41465.5 47 40571.0 48 40616.5 49 39433.0 50 37912.0 51 35517.5 52 33418.0 53 32922.5 54 31910.5 55 30027.0 56 26962.0 57 24351.5 58 22798.0 59 20563.5 60 17728.5 61 14690.0 62 12096.5 63 9414.0 64 7225.5 65 6165.0 66 4753.0 67 3632.5 68 2761.0 69 1940.5 70 1543.5 71 1256.0 72 1039.0 73 900.5 74 744.0 75 581.0 76 342.0 77 217.0 78 157.5 79 83.5 80 51.0 81 30.5 82 17.5 83 9.5 84 3.0 85 0.5 86 0.5 87 2.0 88 3.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06802892928040026 2 7.839441274309889E-4 3 2.61314709143663E-4 4 8.710490304788766E-5 5 0.07116470579012422 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.306573545718315E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002308279930769023 44-45 0.009929958947459194 46-47 0.010626798171842295 48-49 0.00862338540174088 50-51 0.007273259404498619 52-53 0.006053790761828192 54-55 0.007142602049926788 56-57 0.005313399085921148 58-59 0.002874461800580293 60-61 0.0019163078670535285 62-63 0.0012194686426704273 64-65 0.0018292029640056408 66-67 0.002351832382292967 68-69 0.001263021094194371 70-71 0.002569594639912686 72-73 0.002351832382292967 74-75 5.661818698112698E-4 76-77 5.22629418287326E-4 78-79 0.0 80-81 2.61314709143663E-4 82-83 0.0 84-85 8.710490304788766E-5 86-87 2.61314709143663E-4 88-89 0.001263021094194371 90-91 0.0016549931579098655 92-93 4.355245152394383E-4 94-95 0.001306573545718315 96-97 0.0011759161911464836 98-99 0.002090517673149304 100-101 5.661818698112698E-4 102-103 7.839441274309889E-4 104-105 6.097343213352137E-4 106-107 3.4841961219155064E-4 108-109 0.001045258836574652 110-111 8.710490304788766E-4 112-113 0.01101877023555779 114-115 0.01999057524949022 116-117 0.019163078670535287 118-119 0.011192980041653564 120-121 0.006010238310304249 122-123 0.006271553019447911 124-125 0.005139189279825372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1148041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.390935721521803 #Duplication Level Percentage of deduplicated Percentage of total 1 44.05917129893145 12.068219289925498 2 16.577929342144945 9.081699940132449 3 11.957945802573654 9.826179745192091 4 8.267037004199846 9.057675167579205 5 5.577817872314497 7.6390825403460925 6 3.6002979836746856 5.916931838949472 7 2.4091431692421885 4.619207998485927 8 1.6092840034873077 3.5263835757755295 9 1.1027980826142219 2.7186004255233276 >10 4.373613173056344 20.74524310552769 >50 0.31641436674275075 5.977766367718586 >100 0.14404644954485366 6.812708402928962 >500 0.003536854729091657 0.6747095668217377 >1k 6.430644961984831E-4 0.7653343082731888 >5k 3.2153224809924153E-4 0.5702577268203565 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6475 0.5640042472350726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4557 0.39693704318922407 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4133 0.3600045642969197 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.355245152394383E-5 0.0 24-25 0.0 0.0 0.0 8.710490304788766E-5 0.0 26-27 0.0 0.0 0.0 2.1776225761971916E-4 0.0 28-29 0.0 0.0 0.0 3.0486716066760683E-4 0.0 30-31 0.0 0.0 0.0 0.0016985456094338095 0.0 32-33 0.0 0.0 0.0 0.005966685858780305 0.0 34-35 0.0 0.0 0.0 0.013283497714802868 0.0 36-37 0.0 0.0 0.0 0.025260421883887423 0.0 38-39 0.0 0.0 0.0 0.04564296919709314 0.0 40-41 0.0 0.0 0.0 0.06868221605325942 0.0 42-43 0.0 0.0 0.0 0.09612026051334403 0.0 44-45 0.0 0.0 0.0 0.12804420748039486 0.0 46-47 0.0 0.0 0.0 0.17137889674671897 0.0 48-49 0.0 0.0 0.0 0.22007053755048817 0.0 50-51 0.0 0.0 0.0 0.26854441609663765 0.0 52-53 0.0 0.0 0.0 0.32346405746833085 0.0 54-55 0.0 0.0 0.0 0.39153653920025505 0.0 56-57 0.0 0.0 0.0 0.4711068681345004 0.0 58-59 0.0 0.0 0.0 0.5616959673043036 0.0 60-61 0.0 0.0 0.0 0.659863193039273 0.0 62-63 0.0 0.0 0.0 0.7598160692867241 0.0 64-65 0.0 0.0 0.0 0.879323996268426 0.0 66-67 0.0 0.0 0.0 1.0248327368099224 0.0 68-69 0.0 0.0 0.0 1.182492611326599 0.0 70-71 0.0 0.0 0.0 1.356789522325422 0.0 72-73 0.0 0.0 0.0 1.5402760005957976 0.0 74-75 0.0 0.0 0.0 1.739136494254125 0.0 76-77 0.0 0.0 0.0 1.9747552569986613 0.0 78-79 0.0 0.0 0.0 2.243343225546823 0.0 80-81 0.0 0.0 0.0 2.5251711393582634 0.0 82-83 0.0 0.0 0.0 2.8231134602335635 0.0 84-85 0.0 0.0 0.0 3.1575091830344038 0.0 86-87 0.0 0.0 0.0 3.516686250752368 0.0 88-89 0.0 0.0 0.0 3.9023867614484153 0.0 90-91 0.0 0.0 0.0 4.31765938672922 0.0 92-93 0.0 0.0 0.0 4.7543162657082805 0.0 94-95 0.0 0.0 0.0 5.218585398953522 0.0 96-97 0.0 0.0 0.0 5.7300653896507185 0.0 98-99 0.0 0.0 0.0 6.2906289932154 0.0 100-101 0.0 0.0 0.0 6.875015787763678 0.0 102-103 0.0 0.0 0.0 7.468679254486556 0.0 104-105 0.0 0.0 0.0 8.084249604325978 0.0 106-107 0.0 0.0 0.0 8.747814755744786 0.0 108-109 0.0 0.0 0.0 9.447571994379992 0.0 110-111 0.0 0.0 0.0 10.199722832198502 0.0 112-113 0.0 0.0 0.0 10.940942004684501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4125 0.0 86.3906 1 GTATCAA 6020 0.0 58.946808 2 GTACATG 9415 0.0 45.95212 1 CATGGGA 6640 0.0 44.386166 4 TACATGG 9645 0.0 44.323364 2 ACATGGG 9835 0.0 43.709248 3 ATCAACG 8545 0.0 42.712887 4 TCAACGC 8560 0.0 42.63804 5 CAACGCA 8635 0.0 42.30531 6 AACGCAG 8840 0.0 41.391552 7 TATCAAC 9085 0.0 40.305164 3 ATGGGAT 2755 0.0 39.333294 5 ATGGGAG 2380 0.0 39.27654 5 ACGCAGA 10180 0.0 35.709373 8 CGCAGAG 10355 0.0 34.99097 9 CATGGGG 2830 0.0 33.241543 4 ATGGGAC 1270 0.0 29.535706 5 GAGTACA 6170 0.0 29.264877 1 AGTACAT 6060 0.0 28.689352 2 TGGGATA 885 0.0 28.235458 6 >>END_MODULE