FastQCFastQC Report
Thu 26 May 2016
SRR936271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15912
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2361.4831573655103065No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.42106586224233283No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.3205128205128205No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.301659125188537No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA470.29537456008044244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT390.24509803921568626No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG300.1885369532428356No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1885369532428356No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1885369532428356No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.16339869281045752No Hit
GTACATGGGCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC260.16339869281045752No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14454499748617397No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT220.13826043237807945No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.13826043237807945No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.13826043237807945No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1256913021618904No Hit
GTACATGGGCCCAAATAGCTGGGATTACAGGCGTGCACCACCATGCCCAG200.1256913021618904No Hit
ACGCAGAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA200.1256913021618904No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA160.10055304172951231No Hit
GTGAAAACCCATCTCTACTAAAAATACAAAAAAATTTGCTGGGTGTGGTG160.10055304172951231No Hit
GTACATGGGCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT160.10055304172951231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG152.6562912E-4118.955974
TAACAAC303.4249751E-799.129981
GAGAACA807.2759576E-1266.9127351
ATACCTG150.00420498159.477985110-111
GAATCAA350.007667637450.9811362
GAAAACA1450.049.223161
ACAACAC1352.3646862E-1144.057775
AGAACAA951.3715908E-743.8258862
AAACAAC1551.8189894E-1242.2101823
GAACAAA853.2770313E-641.9844673
AACAACA1653.6379788E-1239.6519934
GAAACAA600.001272315539.6519932
CAACACA1601.4551915E-1037.173746
CACAAAA1753.8016879E-1033.9874239
AACACAA2151.8189894E-1233.1970187
ACACAAA1951.2132659E-930.5015328
AAAGAGA450.00940989726.434662118-119
GTACATG2203.7785176E-621.6283591
GAGTACA2203.7785176E-621.6283591
TACATGG2055.4955613E-520.3095572
Produced by FastQC (version 0.11.5)