##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936271_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15912 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.281485671191554 27.0 12.0 31.0 2.0 34.0 2 22.49402966314731 27.0 12.0 31.0 2.0 34.0 3 22.74333836098542 27.0 12.0 31.0 2.0 34.0 4 24.872486173956762 31.0 12.0 37.0 2.0 37.0 5 26.773629964806435 32.0 20.0 37.0 2.0 37.0 6 25.035005027652087 31.0 10.0 37.0 2.0 37.0 7 25.605580693815988 31.0 13.0 37.0 2.0 37.0 8 24.516716943187532 31.0 8.0 37.0 2.0 37.0 9 24.595525389643036 31.0 8.0 39.0 2.0 39.0 10-11 25.376068376068375 31.0 9.0 39.0 2.0 39.0 12-13 25.432189542483663 31.5 9.0 39.0 2.0 39.0 14-15 26.152055052790345 32.0 8.0 40.0 2.0 41.0 16-17 26.316427853192558 32.0 9.5 40.0 2.0 41.0 18-19 26.520456259426847 32.0 12.0 40.0 2.0 41.0 20-21 26.788273001508294 32.0 14.0 40.0 2.0 41.0 22-23 26.941899195575665 31.0 17.0 39.5 2.0 41.0 24-25 27.00996103569633 31.0 18.5 39.0 2.0 41.0 26-27 26.78525641025641 31.0 17.0 39.0 2.0 41.0 28-29 26.3736802413273 30.0 10.5 39.0 2.0 41.0 30-31 25.85027023629965 30.0 2.0 39.0 2.0 41.0 32-33 25.303481649069887 30.0 2.0 39.0 2.0 41.0 34-35 24.74795751633987 30.0 2.0 39.0 2.0 41.0 36-37 24.108220211161388 30.0 2.0 39.0 2.0 41.0 38-39 23.562248617395674 30.0 2.0 39.0 2.0 41.0 40-41 23.35036450477627 30.0 2.0 38.5 2.0 41.0 42-43 22.969017094017094 30.0 2.0 38.5 2.0 41.0 44-45 22.78993841126194 30.0 2.0 38.0 2.0 41.0 46-47 22.596122423328303 30.0 2.0 38.0 2.0 41.0 48-49 22.349578934137757 29.5 2.0 38.0 2.0 41.0 50-51 22.044086224233283 29.0 2.0 38.0 2.0 41.0 52-53 21.805869783810962 29.0 2.0 38.0 2.0 40.5 54-55 21.6327614379085 29.0 2.0 38.0 2.0 40.0 56-57 21.358534439416793 29.0 2.0 37.0 2.0 40.0 58-59 21.088612368024133 28.5 2.0 37.0 2.0 40.0 60-61 20.793080693815988 28.0 2.0 36.5 2.0 40.0 62-63 20.441396430367018 27.5 2.0 36.0 2.0 40.0 64-65 20.14218828557064 27.0 2.0 36.0 2.0 40.0 66-67 19.850647310206135 26.5 2.0 35.0 2.0 39.0 68-69 19.56319130216189 26.0 2.0 35.0 2.0 39.0 70-71 19.171160130718953 25.5 2.0 35.0 2.0 38.5 72-73 18.841000502765212 25.0 2.0 35.0 2.0 38.0 74-75 18.479323780794367 24.0 2.0 35.0 2.0 37.0 76-77 18.205630970336856 22.5 2.0 34.0 2.0 37.0 78-79 17.95871040723982 20.5 2.0 34.0 2.0 36.0 80-81 17.74666918049271 20.0 2.0 34.0 2.0 36.0 82-83 17.55191050779286 19.5 2.0 34.0 2.0 35.5 84-85 17.528657616892914 19.0 2.0 34.0 2.0 35.0 86-87 17.41308446455505 16.5 2.0 34.0 2.0 35.0 88-89 17.268130970336852 12.5 2.0 34.0 2.0 35.0 90-91 17.136814982403216 9.0 2.0 34.0 2.0 35.0 92-93 17.040975364504774 7.0 2.0 34.0 2.0 35.0 94-95 16.918677727501255 5.0 2.0 34.0 2.0 35.0 96-97 16.805272750125688 2.0 2.0 34.0 2.0 35.0 98-99 16.75402212166918 2.0 2.0 34.0 2.0 35.0 100-101 16.59228883861237 2.0 2.0 34.0 2.0 35.0 102-103 16.00443061840121 2.0 2.0 32.5 2.0 34.5 104-105 16.39822146807441 2.0 2.0 34.0 2.0 35.0 106-107 16.425150829562597 2.0 2.0 34.0 2.0 35.0 108-109 16.35787456008044 2.0 2.0 34.0 2.0 35.0 110-111 16.284816490698844 2.0 2.0 34.0 2.0 35.0 112-113 16.175936400201106 2.0 2.0 34.0 2.0 35.0 114-115 16.049522373051786 2.0 2.0 34.0 2.0 35.0 116-117 15.92229135243841 2.0 2.0 34.0 2.0 35.0 118-119 15.715906234288587 2.0 2.0 34.0 2.0 35.0 120-121 15.569601558572147 2.0 2.0 34.0 2.0 35.0 122-123 15.243715434891906 2.0 2.0 34.0 2.0 35.0 124-125 14.134709653092006 2.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.1837151852560126 1101 2 2.183987850375484 1101 3 2.299531839653419 1101 4 3.588640996272776 1101 5 3.3136123012133787 1101 6 2.9789198360724356 1101 7 3.7319085709029025 1101 8 3.4801578051448026 1101 9 4.974545760024583 1101 10-11 2.758943317678547 1101 12-13 2.3319554417797512 1101 14-15 4.8095467091624755 1101 16-17 2.9267370839655804 1101 18-19 3.07182091422645 1101 20-21 3.8590118381036795 1101 22-23 4.548230193409456 1101 24-25 3.275400092987301 1101 26-27 3.6376739655943915 1101 28-29 4.618663813396349 1101 30-31 5.317375499196277 1101 32-33 6.234147951443738 1101 34-35 6.223453608541803 1101 36-37 4.756601690658396 1101 38-39 4.99811867046968 1101 40-41 5.867088415282524 1101 42-43 6.061875057073646 1101 44-45 6.130510976129706 1101 46-47 6.803824423934525 1101 48-49 6.853596614889604 1101 50-51 4.447829173757068 1101 52-53 4.845234652467781 1101 54-55 5.235711796812176 1101 56-57 4.021126357108198 1101 58-59 3.284268345531988 1101 60-61 3.9874951897785067 1101 62-63 3.8608675626428273 1101 64-65 3.7481714776192767 1101 66-67 3.984339542981896 1101 68-69 4.064835017400139 1101 70-71 4.708013716390777 1101 72-73 4.5714812115783126 1101 74-75 4.050229050491598 1101 76-77 4.166272150996367 1101 78-79 4.949129823234955 1101 80-81 4.522883824048147 1101 82-83 3.7944503435659023 1101 84-85 3.9064664469444494 1101 86-87 3.301500248267665 1101 88-89 4.569845433623897 1101 90-91 5.14967627395189 1101 92-93 4.565489185889771 1101 94-95 4.286421330022176 1101 96-97 4.367750926336598 1101 98-99 4.676359642185464 1101 100-101 4.213228736281643 1101 102-103 3.4679575788174795 1101 104-105 3.628536609198971 1101 106-107 4.065855309000334 1101 108-109 4.1193199287130255 1101 110-111 3.3712041963455768 1101 112-113 3.45174490605449 1101 114-115 3.8370340388491897 1101 116-117 3.234028643265141 1101 118-119 3.0321730274278966 1101 120-121 2.906517644829057 1101 122-123 2.1341335454470816 1101 124-125 2.8022207224782285 1102 1 -2.5800210784802537 1102 2 -1.4835945672069357 1102 3 -1.826841786720209 1102 4 -4.230040322408541 1102 5 -3.252321764720687 1102 6 -2.367234010081411 1102 7 -1.8697397807454479 1102 8 -3.4401718651848654 1102 9 -1.5419377564589318 1102 10-11 -2.8742984405632086 1102 12-13 -2.779308294483986 1102 14-15 -3.062706038090269 1102 16-17 -0.8809552237267262 1102 18-19 -1.1218604044548641 1102 20-21 -1.114889260797419 1102 22-23 -0.775945630766369 1102 24-25 -0.22047902789182316 1102 26-27 -0.03265570473528001 1102 28-29 -1.3346328899003517 1102 30-31 -1.457349775529 1102 32-33 -0.3729949056991195 1102 34-35 0.40202503711323345 1102 36-37 0.1989093829660895 1102 38-39 0.24399779134880362 1102 40-41 0.9728576460517573 1102 42-43 0.7789080241066166 1102 44-45 1.12639009700883 1102 46-47 1.016736511846613 1102 48-49 1.136563647856633 1102 50-51 0.6016753276032212 1102 52-53 1.6185863008194268 1102 54-55 1.8359865220868983 1102 56-57 0.8768955878774314 1102 58-59 -0.11683055556691357 1102 60-61 -0.2089333816500627 1102 62-63 -1.6006708988956326 1102 64-65 -1.8150153355675407 1102 66-67 -1.9222538636115125 1102 68-69 -2.2071430045778833 1102 70-71 -1.6793489209718615 1102 72-73 -1.3859363708392713 1102 74-75 -2.5555401802776316 1102 76-77 -1.5713652116409946 1102 78-79 -1.5865844624793297 1102 80-81 -1.516951340787017 1102 82-83 -0.9734067992912436 1102 84-85 -1.3160610255830214 1102 86-87 -1.8441041473367328 1102 88-89 -1.4850996213211545 1102 90-91 -1.1841149348393145 1102 92-93 -1.0869833415827586 1102 94-95 -2.0487435051426637 1102 96-97 -1.4138424802568093 1102 98-99 -1.441772225946405 1102 100-101 -1.632925109872204 1102 102-103 -0.5389105530506519 1102 104-105 -1.3371227314603686 1102 106-107 -1.1470567789117503 1102 108-109 -0.4960646866715912 1102 110-111 -0.19472986958849248 1102 112-113 -0.5166616873521015 1102 114-115 -1.384119807304657 1102 116-117 -0.7275098182733206 1102 118-119 -1.054365434110565 1102 120-121 0.19910006241147293 1102 122-123 0.25913354544708156 1102 124-125 -0.25959246433495764 1103 1 0.8345088360496611 1103 2 0.21441113079876217 1103 3 1.2201667602883397 1103 4 1.6389055465373268 1103 5 3.6522366398377173 1103 6 1.5715124286650273 1103 7 0.312596401590735 1103 8 0.40740648239347976 1103 9 0.26290554838437075 1103 10-11 2.768863952599183 1103 12-13 2.1302358650601754 1103 14-15 1.1210546456704122 1103 16-17 0.8711815284100233 1103 18-19 1.1730113904169315 1103 20-21 1.630837234929075 1103 22-23 -0.33007668489742414 1103 24-25 -0.7781713355841298 1103 26-27 -0.8517440238235956 1103 28-29 -1.0347753400428026 1103 30-31 -0.9260107441899699 1103 32-33 0.3584865757823614 1103 34-35 0.7909139260021227 1103 36-37 -0.41932423526753126 1103 38-39 -0.35571730836629456 1103 40-41 -0.7393930661989572 1103 42-43 -0.4447386995401068 1103 44-45 -0.88073241011368 1103 46-47 -0.8158845707744717 1103 48-49 -1.1953451840521971 1103 50-51 -0.9040226780947869 1103 52-53 -0.5231515909184665 1103 54-55 -1.4382300021296253 1103 56-57 -2.39091067992884 1103 58-59 -1.075520014256373 1103 60-61 -1.6904148631315437 1103 62-63 -1.0002435484682834 1103 64-65 -2.4766962472484515 1103 66-67 -0.424390615748262 1103 68-69 -0.2605618079966874 1103 70-71 -0.20071644233938102 1103 72-73 0.3327245978216986 1103 74-75 -0.6303265050639588 1103 76-77 -2.299997690273475 1103 78-79 -0.8551029809978488 1103 80-81 -0.28333310716878657 1103 82-83 -1.5289623548468008 1103 84-85 -0.6914171509391487 1103 86-87 -0.5278648310974141 1103 88-89 0.024871888650352503 1103 90-91 0.21118421045982672 1103 92-93 -0.19310869770811223 1103 94-95 -0.8379172943164512 1103 96-97 -2.128942195356526 1103 98-99 -0.8527408869150683 1103 100-101 -1.934919411866506 1103 102-103 -1.340904855044954 1103 104-105 -0.7901141844518218 1103 106-107 -0.8885097703647418 1103 108-109 -0.6862356268425298 1103 110-111 0.16353223867361422 1103 112-113 -0.18119160188201633 1103 114-115 -0.5472252204100698 1103 116-117 -0.5715269122904161 1103 118-119 -0.39838252812765873 1103 120-121 -0.9248315615201523 1103 122-123 0.09246687878041548 1103 124-125 0.022458817716326962 1104 1 -4.88771338617256 1104 2 -2.845774054386421 1104 3 -2.807611017489439 1104 4 -2.9351685275367494 1104 5 -3.9542448416437637 1104 6 -5.4794134972608965 1104 7 -3.5716628576685245 1104 8 -4.84722314723615 1104 9 -3.644501859023034 1104 10-11 -1.9464138251785954 1104 12-13 -0.8049493201250151 1104 14-15 -1.7053342432184735 1104 16-17 -1.7190962493677553 1104 18-19 -3.2644886095830685 1104 20-21 -4.2094405428487 1104 22-23 -4.320817425638165 1104 24-25 -3.319838002250794 1104 26-27 -2.210540320119893 1104 28-29 -2.363479043746505 1104 30-31 -1.902862596041821 1104 32-33 -2.9771615723657874 1104 34-35 -3.7854749628867665 1104 36-37 -4.38923164267494 1104 38-39 -3.844143234292222 1104 40-41 -5.257911584717476 1104 42-43 -5.426220181021591 1104 44-45 -4.3992509286321955 1104 46-47 -4.1515327189226205 1104 48-49 -4.241641480348491 1104 50-51 -6.135504159576268 1104 52-53 -5.416670109436982 1104 54-55 -5.151192965092591 1104 56-57 -5.874706976225136 1104 58-59 -5.834779273515629 1104 60-61 -4.917266714983398 1104 62-63 -5.451632437357173 1104 64-65 -4.888733284285486 1104 66-67 -6.479946171303819 1104 68-69 -6.607784030218911 1104 70-71 -5.672938664561606 1104 72-73 -5.669590216993118 1104 74-75 -5.408104282841736 1104 76-77 -5.76527546805125 1104 78-79 -6.419917795812662 1104 80-81 -6.7300923664280425 1104 82-83 -6.410906799291244 1104 84-85 -5.867343076865074 1104 86-87 -7.305642608875193 1104 88-89 -7.340868852090388 1104 90-91 -6.740204678429063 1104 92-93 -6.99403462363404 1104 94-95 -7.04393581283497 1104 96-97 -6.92986812128245 1104 98-99 -6.162926072100252 1104 100-101 -5.661771263718357 1104 102-103 -6.171923373563471 1104 104-105 -6.463725295562931 1104 106-107 -6.0621208814758525 1104 108-109 -6.1931800712869745 1104 110-111 -7.313319613178237 1104 112-113 -7.917302712993129 1104 114-115 -7.4962992944841425 1104 116-117 -7.099304690068193 1104 118-119 -6.946993639238771 1104 120-121 -6.855387117075706 1104 122-123 -6.490866454552918 1104 124-125 -5.852541182283673 1105 1 -0.733867232326407 1105 2 -0.7143637979761657 1105 3 0.019312059433637785 1105 4 -0.7300403224085414 1105 5 -0.9446294570283804 1105 6 -3.828772471619871 1105 7 -3.138970549976218 1105 8 -3.170941095954099 1105 9 -5.465014679535855 1105 10-11 -3.9704522867170553 1105 12-13 -3.702385217560913 1105 14-15 -2.947321422705656 1105 16-17 -2.6501859929574962 1105 18-19 -2.006475789070251 1105 20-21 -2.4802738761820358 1105 22-23 -3.0836379384586756 1105 24-25 -1.9127867201995166 1105 26-27 -1.1288095508891267 1105 28-29 -0.738479043746505 1105 30-31 -0.8035036216828466 1105 32-33 -1.4691487518529662 1105 34-35 -1.6364365013483066 1105 36-37 -1.4357060016492973 1105 38-39 -1.8329252855742766 1105 40-41 -1.5656038924097828 1105 42-43 -3.7595535143549235 1105 44-45 -3.0851483645296334 1105 46-47 -3.5794173343072373 1105 48-49 -3.1711286598356736 1105 50-51 -2.1290939031660088 1105 52-53 -2.9198752376421098 1105 54-55 -3.5870904009900215 1105 56-57 -3.142335181353342 1105 58-59 -2.251445940182297 1105 60-61 -1.9397026124192962 1105 62-63 -3.2737478219725595 1105 64-65 -3.084246104798307 1105 66-67 -3.8453307866884323 1105 68-69 -3.7071430045778833 1105 70-71 -3.256271997894938 1105 72-73 -3.597474832377733 1105 74-75 -4.151694026431478 1105 76-77 -4.225211365487148 1105 78-79 -5.06735369324856 1105 80-81 -5.19002826386394 1105 82-83 -4.55032987621432 1105 84-85 -4.796830256352255 1105 86-87 -4.940257993490578 1105 88-89 -5.119715005936541 1105 90-91 -5.030268780993165 1105 92-93 -4.952367956967372 1105 94-95 -4.683358889758047 1105 96-97 -4.413842480256811 1105 98-99 -4.518695302869482 1105 100-101 -5.709848186795281 1105 102-103 -5.058141322281422 1105 104-105 -4.260199654537292 1105 106-107 -4.41628754814252 1105 108-109 -5.303756994363898 1105 110-111 -4.348576023434646 1105 112-113 -4.478200148890565 1105 114-115 -4.153350576535427 1105 116-117 -3.515971356734859 1105 118-119 -3.285134664879795 1105 120-121 -2.7432076298962205 1105 122-123 -2.587020300706765 1105 124-125 -2.278823233565724 1106 1 -1.9710467195058925 1106 2 -1.387440721053089 1106 3 0.6507223158438933 1106 4 -1.6435018608700815 1106 5 -1.2042448416437637 1106 6 -1.3127468305942323 1106 7 -0.9466628576685245 1106 8 -0.22222314723615 1106 9 -1.2278351923563697 1106 10-11 -0.0505804918452597 1106 12-13 -0.2841159867916794 1106 14-15 -0.642834243218477 1106 16-17 -0.6149295827010874 1106 18-19 -1.0144886095830685 1106 20-21 -0.06360720951537147 1106 22-23 -0.2583174256381646 1106 24-25 -3.819838002250794 1106 26-27 -2.0022069867865575 1106 28-29 -2.800979043746505 1106 30-31 -2.048695929375153 1106 32-33 -3.9979949056991195 1106 34-35 -1.5563082962201022 1106 36-37 -2.0767316426749396 1106 38-39 -2.26080990095889 1106 40-41 -2.132911584717476 1106 42-43 -3.4887201810215878 1106 44-45 -3.920084261965531 1106 46-47 -3.1723660522559562 1106 48-49 -2.179141480348491 1106 50-51 -2.1980041595762643 1106 52-53 -2.27083677610365 1106 54-55 -1.6511929650925872 1106 56-57 -2.166373642891802 1106 58-59 -0.8556126068489647 1106 60-61 -0.9797667149833984 1106 62-63 -0.43079910402383703 1106 64-65 -0.11789995095215389 1106 66-67 -0.2924461713038191 1106 68-69 -0.3786173635522445 1106 70-71 -0.06877199789493815 1106 72-73 0.4137431163402141 1106 74-75 -0.24143761617506598 1106 76-77 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1.5213775229183497 2216 2 0.7261956425832743 2216 3 0.2605708006923777 2216 4 2.367861775493555 2216 5 2.1707551583562363 2216 6 2.160738017890619 2216 7 -2.673935584941251 2216 8 1.2626253376123344 2216 9 -1.220259434780612 2216 10-11 -0.07709564336041552 2216 12-13 -0.9261614413371326 2216 14-15 -1.2602584856427157 2216 16-17 -0.4596265523980527 2216 18-19 3.203314420719959 2216 20-21 2.156089760181601 2216 22-23 3.1488795440588078 2216 24-25 2.8127377553249637 2216 26-27 2.6928687707891967 2216 28-29 2.8978845926171317 2216 30-31 2.456985888806667 2216 32-33 2.9451869124827006 2216 34-35 2.03838867347687 2216 36-37 -0.6524892184325104 2216 38-39 0.3925991899502037 2216 40-41 0.8784520516461605 2216 42-43 0.9222646674632564 2216 44-45 1.5022642228829568 2216 46-47 1.626876371986473 2216 48-49 2.0613888226818062 2216 50-51 1.9932837192116146 2216 52-53 1.5871177693508969 2216 54-55 2.045776731877112 2216 56-57 2.009762720744561 2216 58-59 1.1121904234540665 2216 60-61 1.3137938910772107 2216 62-63 1.4972311990064604 2216 64-65 1.7059636854114792 2216 66-67 2.9518720105143608 2216 68-69 1.8505493031144233 2216 70-71 2.1300916384686985 2216 72-73 1.5633643284614287 2216 74-75 1.6979563232188717 2216 76-77 2.3010124107366288 2216 78-79 0.2770519011570336 2216 80-81 0.35892278508710973 2216 82-83 0.5720477461633031 2216 84-85 0.4811417716197717 2216 86-87 0.830721027488444 2216 88-89 0.19322205700051853 2216 90-91 -0.1474016481260314 2216 92-93 -0.08873159333100489 2216 94-95 -0.8337085401076969 2216 96-97 -0.5624438788582111 2216 98-99 -0.9697442539184316 2216 100-101 -0.30949853644563063 2216 102-103 -0.5791203432604419 2216 104-105 -0.4315283258659619 2216 106-107 -0.7344693663243369 2216 108-109 -0.6988618894687928 2216 110-111 -0.7489256737842958 2216 112-113 -1.0726057432961582 2216 114-115 -2.8788750520599002 2216 116-117 -2.9932440840075856 2216 118-119 -2.6837360634811933 2216 120-121 -2.3743265110151004 2216 122-123 -1.2635937272801918 2216 124-125 -0.18966239440488586 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2188.0 3 294.0 4 255.0 5 458.0 6 983.0 7 835.0 8 408.0 9 361.0 10 324.0 11 284.0 12 245.0 13 219.0 14 235.0 15 186.0 16 129.0 17 120.0 18 102.0 19 89.0 20 94.0 21 66.0 22 78.0 23 155.0 24 253.0 25 223.0 26 270.0 27 243.0 28 249.0 29 239.0 30 264.0 31 315.0 32 444.0 33 547.0 34 632.0 35 898.0 36 1316.0 37 1451.0 38 454.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.7526395173454 22.10910005027652 9.973604826546003 15.164655605832076 2 18.250377073906485 32.23353443941679 29.430618401206637 20.085470085470085 3 18.250377073906485 43.539467068878835 21.945701357466064 16.264454499748616 4 9.873051784816491 28.544494720965307 24.91830065359477 36.66415284062342 5 8.251633986928104 56.93815987933635 23.127199597787833 11.683006535947712 6 22.102815485168424 44.73353443941679 14.498491704374059 18.665158371040725 7 21.45550527903469 49.43438914027149 13.895173453996984 15.214932126696832 8 20.933886375062848 49.18300653594771 12.98391151332328 16.899195575666166 9 20.95274007038713 32.15811965811966 12.895927601809957 33.99321266968326 10-11 18.408069635169532 44.210162461113036 17.301951418785155 20.07981648493228 12-13 20.463187003110956 44.16302674166484 17.77645099456368 17.59733526066053 14-15 21.896112874336172 44.00905005813405 16.428369418345223 17.666467649184554 16-17 19.178555716171203 43.403934385016655 19.153415875809188 18.264094023002954 18-19 19.216265476714224 40.32430394066998 22.141914398843568 18.317516183772234 20-21 22.833260008798945 37.31066557727358 22.116774558481552 17.739299855445918 22-23 22.10655428355768 39.41376273745125 20.18178387218518 18.297899106805886 24-25 20.2432203123527 39.1289319045973 20.601451780159007 20.02639600289099 26-27 20.554961975991453 37.82603230469487 21.28401734648985 20.33498837282383 28-29 20.179738562091504 41.343011563599795 20.852187028657617 17.625062845651083 30-31 20.35570638511815 41.6289592760181 20.864756158873806 17.150578179989946 32-33 18.471497705989567 42.124945006599205 21.629690151467535 17.773867135943686 34-35 18.042986425339365 43.872549019607845 20.443690296631473 17.640774258421317 36-37 18.159250879839114 43.77513826043238 19.64555052790347 18.420060331825038 38-39 18.37292609351433 43.504901960784316 19.812091503267972 18.31008044243338 40-41 17.82931121166415 45.26772247360483 19.856083459024635 17.046882855706386 42-43 17.813599798893918 45.930744092508796 19.59841628959276 16.657239819004523 44-45 17.917295123177475 45.38712921065862 19.937782805429865 16.757792860734035 46-47 16.314731020613372 45.85532931121166 20.773629964806435 17.056309703368527 48-49 16.61639014580191 44.65183509301156 19.777526395173453 18.954248366013072 50-51 16.804927099044747 44.16478129713424 20.038335847159377 18.99195575666164 52-53 16.966249764313996 43.6679027088178 18.889447552008047 20.47639997486016 54-55 16.660905634289666 43.393143324010936 19.199949721899255 20.746001319800143 56-57 17.575338591584703 42.78352135248091 18.244665807749115 21.396474248185275 58-59 17.69733534439417 40.9973604826546 19.274761186525893 22.03054298642534 60-61 18.24409250879839 40.1395173453997 18.878833584715938 22.737556561085974 62-63 18.338360985419808 38.66264454499749 19.601558572146807 23.397435897435898 64-65 19.96920563097034 36.990950226244344 19.928355957767725 23.1114881850176 66-67 21.436651583710407 36.95324283559578 19.469582704876824 22.14052287581699 68-69 22.31963298139769 36.1770990447461 20.32114127702363 21.18212669683258 70-71 22.982654600301657 36.08597285067873 21.141277023629964 19.790095525389642 72-73 22.630718954248366 35.545500251382606 22.077677224736046 19.746103569632982 74-75 22.56787330316742 35.11500754147813 22.574157868275517 19.742961287078934 76-77 22.586726998491706 32.91540975364505 23.083207642031173 21.414655605832074 78-79 23.296882855706386 32.88712921065862 23.548265460030166 20.267722473604827 80-81 23.180618401206637 32.90284062342886 24.318124685771743 19.59841628959276 82-83 23.309451985922575 33.242207139265965 22.92609351432881 20.522247360482655 84-85 24.07302664655606 32.01671694318753 24.019607843137255 19.890648567119154 86-87 22.878959276018097 32.50691302161891 23.551407742584214 21.062719959778782 88-89 22.794117647058822 33.85495223730518 22.93552036199095 20.415409753645047 90-91 22.385620915032682 34.464555052790345 22.385620915032682 20.764203117144294 92-93 21.13499245852187 34.92647058823529 22.479889391654098 21.458647561588737 94-95 19.90950226244344 36.8149824032177 22.181372549019606 21.094142785319256 96-97 20.02576671694319 38.48353443941679 20.981020613373556 20.509678230266466 98-99 20.352564102564102 39.589617898441425 19.69896933132227 20.3588486676722 100-101 18.03984414278532 42.12229763700352 20.063474107591755 19.774384112619405 102-103 17.203996983408747 45.69193061840121 18.60545500251383 18.49861739567622 104-105 16.082202111613878 47.21279537456008 18.19695827048768 18.50804424333836 106-107 15.315485168426346 48.80907491201609 18.225238813474107 17.65020110608346 108-109 14.913273001508296 49.19871794871795 18.36035696329814 17.527652086475616 110-111 16.104198089492208 49.211287078934134 17.52136752136752 17.163147310206135 112-113 15.334338863750629 49.24585218702866 18.41691804927099 17.002890899949723 114-115 15.585721468074409 49.060457516339866 17.750754147812973 17.60306686777275 116-117 17.175716440422324 47.49245852187028 17.942433383609853 17.389391654097537 118-119 17.835595776772248 47.13738059326295 17.8324534942182 17.194570135746606 120-121 18.297511312217193 45.55052790346908 18.709150326797385 17.442810457516337 122-123 18.649446958270488 43.49861739567622 19.21191553544495 18.640020110608347 124-125 19.80266465560583 42.58107088989442 18.262946204122674 19.353318250377075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148.0 1 135.5 2 136.5 3 134.5 4 105.0 5 68.0 6 43.0 7 46.0 8 48.5 9 41.5 10 40.5 11 44.0 12 37.0 13 34.0 14 40.0 15 35.5 16 33.0 17 38.5 18 49.0 19 53.5 20 53.5 21 66.0 22 83.5 23 102.5 24 129.5 25 147.5 26 162.0 27 202.5 28 262.5 29 313.5 30 320.0 31 344.0 32 388.0 33 427.0 34 474.0 35 531.5 36 565.0 37 554.0 38 498.0 39 439.0 40 453.0 41 464.5 42 422.0 43 401.0 44 439.0 45 462.5 46 497.0 47 468.5 48 387.5 49 380.5 50 458.5 51 453.0 52 344.0 53 297.0 54 277.5 55 247.5 56 250.5 57 274.5 58 222.5 59 182.0 60 180.0 61 150.5 62 140.5 63 125.0 64 88.0 65 59.0 66 51.5 67 52.5 68 46.5 69 43.0 70 40.0 71 44.0 72 38.0 73 30.0 74 21.0 75 15.0 76 18.0 77 18.0 78 15.5 79 14.0 80 10.5 81 5.5 82 3.0 83 2.0 84 3.5 85 4.0 86 4.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0031422825540472596 12-13 0.0031422825540472596 14-15 0.0031422825540472596 16-17 0.006284565108094519 18-19 0.006284565108094519 20-21 0.006284565108094519 22-23 0.08798391151332328 24-25 0.0031422825540472596 26-27 0.006284565108094519 28-29 0.0 30-31 0.0 32-33 0.006284565108094519 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.006284565108094519 54-55 0.0031422825540472596 56-57 0.0031422825540472596 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 15912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.76646556058321 #Duplication Level Percentage of deduplicated Percentage of total 1 83.89131039599368 56.85017596782303 2 6.751367893907076 9.15032679738562 3 3.5147918019104143 7.145550527903469 4 2.2257256793100253 6.033182503770739 5 1.335435407586015 4.524886877828054 6 0.797551701752759 3.2428355957767727 7 0.46369284985625525 2.199597787833082 8 0.24112028192525273 1.3071895424836601 9 0.16692942594825189 1.0180995475113122 >10 0.5842529908188816 6.303418803418803 >50 0.018547713994250207 0.7415786827551533 >100 0.009273856997125103 1.4831573655103065 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 236 1.4831573655103065 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.42106586224233283 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.3205128205128205 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.301659125188537 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 47 0.29537456008044244 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 39 0.24509803921568626 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 30 0.1885369532428356 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.1885369532428356 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.1885369532428356 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.16339869281045752 No Hit GTACATGGGCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC 26 0.16339869281045752 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.14454499748617397 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22 0.13826043237807945 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.13826043237807945 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.13826043237807945 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.1256913021618904 No Hit GTACATGGGCCCAAATAGCTGGGATTACAGGCGTGCACCACCATGCCCAG 20 0.1256913021618904 No Hit ACGCAGAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 20 0.1256913021618904 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 16 0.10055304172951231 No Hit GTGAAAACCCATCTCTACTAAAAATACAAAAAAATTTGCTGGGTGTGGTG 16 0.10055304172951231 No Hit GTACATGGGCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT 16 0.10055304172951231 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.00314228255404726 0.0 16-17 0.0 0.0 0.0 0.00628456510809452 0.0 18-19 0.0 0.0 0.0 0.01256913021618904 0.0 20-21 0.0 0.0 0.0 0.01885369532428356 0.0 22-23 0.0 0.0 0.0 0.021995977878330818 0.0 24-25 0.0 0.0 0.0 0.04399195575666164 0.0 26-27 0.0 0.0 0.0 0.04399195575666164 0.0 28-29 0.0 0.0 0.0 0.056561085972850686 0.0 30-31 0.0 0.0 0.0 0.10369532428355957 0.0 32-33 0.0 0.0 0.0 0.14768728004022122 0.0 34-35 0.0 0.0 0.0 0.20739064856711914 0.0 36-37 0.0 0.0 0.0 0.2922322775263952 0.0 38-39 0.0 0.0 0.0 0.3770739064856712 0.0 40-41 0.0 0.0 0.0 0.5184766214177978 0.0 42-43 0.0 0.0 0.0 0.6630216189039719 0.0 44-45 0.0 0.0 0.0 0.7981397687280041 0.0 46-47 0.0 0.0 0.0 0.9175465057817999 0.0 48-49 0.0 0.0 0.0 1.0243841126194067 0.0 50-51 0.0 0.0 0.0 1.1280794369029663 0.0 52-53 0.0 0.0 0.0 1.2537707390648567 0.0 54-55 0.0 0.0 0.0 1.379462041226747 0.0 56-57 0.0 0.0 0.0 1.53657616892911 0.0 58-59 0.0 0.0 0.0 1.703117144293615 0.0 60-61 0.0 0.0 0.0 1.8413775766716944 0.0 62-63 0.0 0.0 0.0 1.9419306184012066 0.0 64-65 0.0 0.0 0.0 2.0990447461035693 0.0 66-67 0.0 0.0 0.0 2.2907239819004523 0.0 68-69 0.0 0.0 0.0 2.4478381096028157 0.0 70-71 0.0 0.0 0.0 2.7023629964806437 0.0 72-73 0.0 0.0 0.0 2.9380341880341883 0.0 74-75 0.0 0.0 0.0 3.161136249371544 0.0 76-77 0.0 0.0 0.0 3.362242332830568 0.0 78-79 0.0 0.0 0.0 3.604198089492207 0.0 80-81 0.0 0.0 0.0 3.915284062342886 0.0 82-83 0.0 0.0 0.0 4.22008547008547 0.0 84-85 0.0 0.0 0.0 4.540598290598291 0.0 86-87 0.0 0.0 0.0 4.901960784313726 0.0 88-89 0.0 0.0 0.0 5.209904474610357 0.0 90-91 0.0 0.0 0.0 5.599547511312217 0.0 92-93 0.0 0.0 0.0 5.9012066365007545 0.0 94-95 0.0 0.0 0.0 6.394544997486173 0.0 96-97 0.0 0.0 0.0 6.774761186525892 0.0 98-99 0.0 0.0 0.0 7.114127702362996 0.0 100-101 0.0 0.0 0.0 7.69544997486174 0.0 102-103 0.0 0.0 0.0 8.094519859225741 0.0 104-105 0.0 0.0 0.0 8.547008547008547 0.0 106-107 0.0 0.0 0.0 9.12833081950729 0.0 108-109 0.0 0.0 0.0 9.618526897938661 0.0 110-111 0.0 0.0 0.0 10.2061337355455 0.0 112-113 0.0 0.0 0.0 10.759175465057819 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTAGG 15 2.6562912E-4 118.95597 4 TAACAAC 30 3.4249751E-7 99.12998 1 GAGAACA 80 7.2759576E-12 66.912735 1 ATACCTG 15 0.004204981 59.477985 110-111 GAATCAA 35 0.0076676374 50.981136 2 GAAAACA 145 0.0 49.22316 1 ACAACAC 135 2.3646862E-11 44.05777 5 AGAACAA 95 1.3715908E-7 43.825886 2 AAACAAC 155 1.8189894E-12 42.210182 3 GAACAAA 85 3.2770313E-6 41.984467 3 AACAACA 165 3.6379788E-12 39.651993 4 GAAACAA 60 0.0012723155 39.651993 2 CAACACA 160 1.4551915E-10 37.17374 6 CACAAAA 175 3.8016879E-10 33.987423 9 AACACAA 215 1.8189894E-12 33.197018 7 ACACAAA 195 1.2132659E-9 30.501532 8 AAAGAGA 45 0.009409897 26.434662 118-119 GTACATG 220 3.7785176E-6 21.628359 1 GAGTACA 220 3.7785176E-6 21.628359 1 TACATGG 205 5.4955613E-5 20.309557 2 >>END_MODULE