FastQCFastQC Report
Thu 26 May 2016
SRR936271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15912
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5763.619909502262444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4943.104575163398693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3712.331573655103067No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.7415786827551533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.5027652086475616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG650.40849673202614384No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT620.38964303670186023No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.37078934137757663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC580.36450477626948213No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.33308195072900953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC520.32679738562091504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG490.3079436902966315No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.301659125188537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC410.2576671694318753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC400.2513826043237808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC350.2199597787833082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC350.2199597787833082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC340.2136752136752137No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA340.2136752136752137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.19482151835093012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC310.19482151835093012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.19482151835093012No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC270.16968325791855204No Hit
GTACATGGGCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT270.16968325791855204No Hit
GATTACAGGCGTGCACCACCATGCCCAGATAATTTTGTATTTTTAGTAAA260.16339869281045752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.1508295625942685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.1508295625942685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC240.1508295625942685No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1508295625942685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.14454499748617397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC230.14454499748617397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA220.13826043237807945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG210.13197586726998492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC200.1256913021618904No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.1256913021618904No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.1256913021618904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC180.11312217194570137No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG170.10683760683760685No Hit
GGTATCAACGCAGAGTACATGGAGCCCAACTTAGAGACTTTTTCATTGGC170.10683760683760685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT170.10683760683760685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT160.10055304172951231No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC160.10055304172951231No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT160.10055304172951231No Hit
GGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAAGCTCTGCCTCCCA160.10055304172951231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.070.01
AAATTTC150.004198823659.576-77
ATTCTTC150.004198823659.562-63
GTATCAA5000.047.62
CAACGCA6850.036.481756
TCAACGC6700.036.4104465
ATCAACG6700.036.4104464
AACGCAG6900.036.2173927
TATCAAC6900.035.3550763
ACGCAGA7450.033.5436258
CGCAGAG7500.033.3200049
GAGTACT6000.031.23749712-13
GTACTTT6550.028.61450414-15
CAGAGTA7600.028.1842110-11
AGTACTT6150.027.08943212-13
TTTGGGG450.00939293926.44444334-35
GCAGAGT7850.025.77078
AGAGTAC7550.025.21854410-11
ACTTTTT7450.025.15771916-17
TACTTTT7050.023.63120714-15
Produced by FastQC (version 0.11.5)