##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936271_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15912 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.201168929110107 31.0 31.0 33.0 30.0 34.0 2 30.99459527400704 31.0 31.0 34.0 28.0 34.0 3 31.245663650075414 31.0 31.0 34.0 28.0 34.0 4 35.19859225741579 35.0 35.0 37.0 33.0 37.0 5 34.92370537958773 35.0 35.0 37.0 32.0 37.0 6 35.15208647561589 37.0 35.0 37.0 32.0 37.0 7 34.914529914529915 36.0 35.0 37.0 32.0 37.0 8 35.083584715937654 37.0 35.0 37.0 32.0 37.0 9 36.527400703871294 39.0 35.0 39.0 32.0 39.0 10-11 36.49946581196581 39.0 35.0 39.0 32.0 39.0 12-13 36.38763197586727 39.0 35.0 39.0 31.5 39.0 14-15 36.96515208647561 39.0 36.0 40.5 31.0 41.0 16-17 36.992269984917044 38.5 36.0 40.0 31.5 41.0 18-19 37.52960030165913 39.5 36.5 41.0 32.0 41.0 20-21 37.68966817496229 40.0 37.0 41.0 32.0 41.0 22-23 37.46427224736048 40.0 36.0 41.0 32.0 41.0 24-25 37.277557817998996 39.0 36.0 41.0 31.5 41.0 26-27 36.730989190548016 39.0 35.0 41.0 31.0 41.0 28-29 36.34511689291101 39.0 35.0 40.5 30.0 41.0 30-31 35.47505027652086 37.5 34.5 40.0 29.5 41.0 32-33 34.42031171442936 35.5 33.5 40.0 27.5 41.0 34-35 33.597819255907496 35.0 33.0 39.5 24.5 41.0 36-37 32.777683509301156 35.0 33.0 39.0 19.5 41.0 38-39 32.19299899446958 35.0 33.0 39.0 11.0 41.0 40-41 31.469111362493717 35.0 32.5 40.0 2.0 41.0 42-43 30.630153343388635 35.0 31.0 40.0 2.0 41.0 44-45 29.433226495726494 35.0 27.5 40.0 2.0 41.0 46-47 27.458961789844142 35.0 12.5 39.0 2.0 41.0 48-49 25.85969708396179 35.0 2.0 39.0 2.0 41.0 50-51 25.00810708898944 34.0 2.0 39.0 2.0 41.0 52-53 24.568847410759176 34.0 2.0 39.0 2.0 41.0 54-55 24.15689416792358 33.5 2.0 39.0 2.0 41.0 56-57 23.78663901458019 33.0 2.0 38.5 2.0 41.0 58-59 23.491138763197586 33.0 2.0 38.0 2.0 41.0 60-61 23.269702111613874 33.0 2.0 38.0 2.0 40.0 62-63 23.005247611865258 33.0 2.0 37.5 2.0 40.0 64-65 22.806749622926095 32.0 2.0 37.0 2.0 40.0 66-67 22.559012066365007 32.0 2.0 36.5 2.0 40.0 68-69 22.267628205128204 32.0 2.0 36.0 2.0 39.0 70-71 21.943847410759176 31.0 2.0 35.5 2.0 39.0 72-73 21.70292860734037 31.0 2.0 35.0 2.0 39.0 74-75 21.576074660633484 31.0 2.0 35.0 2.0 38.0 76-77 21.303575917546507 31.0 2.0 35.0 2.0 37.0 78-79 21.064291101055808 30.5 2.0 35.0 2.0 37.0 80-81 20.867458521870287 30.0 2.0 35.0 2.0 36.0 82-83 20.729795123177475 30.0 2.0 35.0 2.0 36.0 84-85 20.453839869281047 30.0 2.0 35.0 2.0 35.5 86-87 20.32975113122172 30.0 2.0 35.0 2.0 35.0 88-89 20.16295877325289 29.0 2.0 35.0 2.0 35.0 90-91 20.07698592257416 29.0 2.0 35.0 2.0 35.0 92-93 19.949252136752136 29.0 2.0 35.0 2.0 35.0 94-95 19.83521870286576 29.0 2.0 35.0 2.0 35.0 96-97 19.75241955756662 29.0 2.0 35.0 2.0 35.0 98-99 19.545720211161388 29.0 2.0 35.0 2.0 35.0 100-101 19.44808949220714 29.0 2.0 35.0 2.0 35.0 102-103 18.700446204122674 25.5 2.0 33.5 2.0 35.0 104-105 19.195010055304174 27.5 2.0 34.0 2.0 35.0 106-107 19.225835847159374 28.0 2.0 35.0 2.0 35.0 108-109 19.14426219205631 27.0 2.0 35.0 2.0 35.0 110-111 19.066867772750125 27.0 2.0 35.0 2.0 35.0 112-113 18.96804298642534 27.0 2.0 35.0 2.0 35.0 114-115 18.88150452488688 27.0 2.0 35.0 2.0 35.0 116-117 18.654506033182507 26.5 2.0 35.0 2.0 35.0 118-119 18.48802790346908 25.0 2.0 35.0 2.0 35.0 120-121 18.241075917546507 24.5 2.0 34.5 2.0 35.0 122-123 17.987745098039216 24.0 2.0 34.0 2.0 35.0 124-125 16.58785822021116 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8150788499625392 1101 2 1.3359152815852617 1101 3 0.813661324632907 1101 4 0.7520175489091727 1101 5 1.036953242315299 1101 6 1.0643113358015626 1101 7 0.8057234982970627 1101 8 0.6615581843402296 1101 9 0.7103936419877641 1101 10-11 0.6665694400898659 1101 12-13 1.2338621155531584 1101 14-15 1.50528646295313 1101 16-17 0.633482480763746 1101 18-19 1.1043681250575972 1101 20-21 1.3382757960417422 1101 22-23 1.4278735159845724 1101 24-25 0.7809841026010034 1101 26-27 0.7807041076054588 1101 28-29 0.9508017055390994 1101 30-31 0.9700188345816017 1101 32-33 1.5228371256165403 1101 34-35 2.3361060041755337 1101 36-37 3.387927567443299 1101 38-39 3.0181035487999495 1101 40-41 1.7812636091801188 1101 42-43 0.776293027351528 1101 44-45 2.0170270800612435 1101 46-47 3.3065091666304554 1101 48-49 3.695112864689502 1101 50-51 3.466685093052181 1101 52-53 4.145901119172766 1101 54-55 3.887146096806486 1101 56-57 4.345698661382986 1101 58-59 4.804672625476776 1101 60-61 4.54473269173841 1101 62-63 4.479356021290055 1101 64-65 4.540130811830998 1101 66-67 3.8094447297736806 1101 68-69 4.2398348351324096 1101 70-71 4.550599461683955 1101 72-73 4.919629045282914 1101 74-75 5.407318469755921 1101 76-77 4.572214353055511 1101 78-79 3.929693311618209 1101 80-81 5.0590917804418645 1101 82-83 5.922907836431186 1101 84-85 5.532282254489726 1101 86-87 4.679359728661176 1101 88-89 5.15291423765299 1101 90-91 4.896568850941332 1101 92-93 5.367634937222878 1101 94-95 5.212708831004548 1101 96-97 5.339024783405819 1101 98-99 5.615156183188368 1101 100-101 4.414355638509388 1101 102-103 4.5680830234805505 1101 104-105 4.991051506047636 1101 106-107 5.1926768925284925 1101 108-109 4.161493370909195 1101 110-111 4.665279680792526 1101 112-113 3.80324930968208 1101 114-115 3.765978471020823 1101 116-117 4.304123325677686 1101 118-119 3.778294848239497 1101 120-121 3.9320321936296594 1101 122-123 3.091383315257765 1101 124-125 3.1751946998862444 1102 1 0.5568370917207766 1102 2 1.1353658310358092 1102 3 0.6295953905669762 1102 4 0.4498197467113698 1102 5 1.0012389566010143 1102 6 0.11650913799936546 1102 7 0.83044877302234 1102 8 0.76320653598858 1102 9 1.383470565064684 1102 10-11 0.6473386708590922 1102 12-13 0.4193016759927133 1102 14-15 0.26902272668939275 1102 16-17 0.7996912719725415 1102 18-19 0.7527197734092468 1102 20-21 0.7558582136241583 1102 22-23 1.084466922577981 1102 24-25 1.1655994872163902 1102 26-27 1.3287810306823786 1102 28-29 1.176076430813822 1102 30-31 1.347766087328857 1102 32-33 0.5681667959462189 1102 34-35 1.572369740439271 1102 36-37 1.6173231718388976 1102 38-39 1.4782683839647817 1102 40-41 0.8142306421471481 1102 42-43 0.8903040163625207 1102 44-45 0.607686420720583 1102 46-47 0.7900256501469407 1102 48-49 0.4409919855686262 1102 50-51 0.6631136644807505 1102 52-53 1.4288681521397955 1102 54-55 1.4352230198834093 1102 56-57 0.5256437163280374 1102 58-59 0.7071451529493089 1102 60-61 0.515886537892257 1102 62-63 0.6194659113999421 1102 64-65 1.2681527898529765 1102 66-67 -0.1493464790175274 1102 68-69 1.1574172527148257 1102 70-71 1.6398851759696704 1102 72-73 1.4979257485796182 1102 74-75 0.8619887994262498 1102 76-77 1.028258309099467 1102 78-79 1.3060669379918366 1102 80-81 0.8022236485737331 1102 82-83 0.6976331111564633 1102 84-85 1.2616778588853315 1102 86-87 1.6038102781117267 1102 88-89 1.3452219299606831 1102 90-91 1.6383270926995692 1102 92-93 1.5242283438162865 1102 94-95 2.0190275123232304 1102 96-97 2.145343464724501 1102 98-99 2.0011451941773792 1102 100-101 2.26325673741049 1102 102-103 2.2026984080959373 1102 104-105 1.919622934619067 1102 106-107 2.330039529891131 1102 108-109 1.5694604038762243 1102 110-111 1.477092867605709 1102 112-113 1.5216559030886714 1102 114-115 0.27971473475708564 1102 116-117 0.150277171831533 1102 118-119 0.4431300130746614 1102 120-121 0.33313109472856084 1102 122-123 0.510339359213809 1102 124-125 1.3743705240620692 1103 1 0.264814299697985 1103 2 1.7208359165058944 1103 3 1.0028147637863505 1103 4 0.7176260145176414 1103 5 0.8160537714158309 1103 6 0.033888055378277215 1103 7 0.79910974168331 1103 8 0.5067962795783245 1103 9 1.6455788271729475 1103 10-11 0.7313842549046754 1103 12-13 0.5916663483573927 1103 14-15 2.37301133067799 1103 16-17 2.1209163431976137 1103 18-19 1.9998707705602428 1103 20-21 2.1226673304332735 1103 22-23 2.2069740450851043 1103 24-25 2.1050581766750796 1103 26-27 1.8038522557536112 1103 28-29 2.24445249918989 1103 30-31 2.6611564007191646 1103 32-33 2.7483662261456416 1103 34-35 3.135047803117331 1103 36-37 4.137266191781919 1103 38-39 4.600775506471898 1103 40-41 3.797136625053131 1103 42-43 3.6089649850234835 1103 44-45 3.366233429267588 1103 46-47 1.0022763623976516 1103 48-49 0.20238799696463516 1103 50-51 -0.2905900392229519 1103 52-53 -1.5511888279171835 1103 54-55 -0.6545205698601784 1103 56-57 -0.5398833491990267 1103 58-59 -0.8719146761105208 1103 60-61 -0.3765636045578873 1103 62-63 -0.3791095871755559 1103 64-65 0.17769694939713432 1103 66-67 -0.43923251890356596 1103 68-69 0.05200414730171943 1103 70-71 0.6398851759696704 1103 72-73 0.7906607913146608 1103 74-75 0.3114189988564533 1103 76-77 0.27327255411371 1103 78-79 0.4164657983906963 1103 80-81 0.09282193917202264 1103 82-83 -0.2411133275899786 1103 84-85 0.4575468047542799 1103 86-87 0.9948359191373655 1103 88-89 1.1806920154307683 1103 90-91 1.3740820784545562 1103 92-93 1.5078465774345169 1103 94-95 1.526862270157988 1103 96-97 1.338363407744442 1103 98-99 1.5602620032941878 1103 100-101 1.1286413527951034 1103 102-103 1.8180830234805505 1103 104-105 1.5307340457301777 1103 106-107 1.7032589031105054 1103 108-109 1.4412552756711001 1103 110-111 1.6672638077766528 1103 112-113 1.8621117435445136 1103 114-115 2.5411107461530946 1103 116-117 2.313382584936946 1103 118-119 2.709511779456431 1103 120-121 2.8844131460106084 1103 122-123 2.533843632718085 1103 124-125 2.585908985600529 1104 1 0.755555040438729 1104 2 0.901391472061448 1104 3 0.6231851341567207 1104 4 0.7222556441472676 1104 5 0.16790562326767855 1104 6 -0.36426009276986804 1104 7 -0.057371739798171006 1104 8 -0.2848703870883398 1104 9 -0.2896063580122359 1104 10-11 -0.29176389324346985 1104 12-13 -0.5935188368277977 1104 14-15 -0.5482849656183006 1104 16-17 -0.7563984716172101 1104 18-19 -0.011703303513833418 1104 20-21 0.576371034136983 1104 22-23 1.308825896936959 1104 24-25 1.2184841026010034 1104 26-27 1.0515374409387945 1104 28-29 1.5222302769676688 1104 30-31 1.6634712155339884 1104 32-33 1.7668847446641678 1104 34-35 0.20217743274696431 1104 36-37 -1.3233819563662266 1104 38-39 -0.9640393083429117 1104 40-41 0.31102551394202393 1104 42-43 0.23165017020867396 1104 44-45 -0.5782110151768514 1104 46-47 -2.5208717857504936 1104 48-49 -3.917982373405735 1104 50-51 -3.1702196688525817 1104 52-53 -4.074336976065329 1104 54-55 -3.4461872365268498 1104 56-57 -2.5352537195693934 1104 58-59 -2.0733035649994136 1104 60-61 -2.7052673082615897 1104 62-63 -2.842072550138518 1104 64-65 -2.986654902454717 1104 66-67 -2.601269555940604 1104 68-69 -2.3345699267723568 1104 70-71 -1.9226148240303296 1104 72-73 -3.2023947642408928 1104 74-75 -2.232562482625031 1104 76-77 -1.960523742182584 1104 78-79 -2.3798304979055978 1104 80-81 -3.080789171939088 1104 82-83 -2.8360207349973834 1104 84-85 -2.6373606026531284 1104 86-87 -3.5884974141959667 1104 88-89 -2.7845857623470103 1104 90-91 -3.4754549585824783 1104 92-93 -3.2514126818247426 1104 94-95 -2.6027673594716454 1104 96-97 -4.226451407070373 1104 98-99 -4.298534293002108 1104 100-101 -4.058858647204897 1104 102-103 -2.9735836431861173 1104 104-105 -3.705377065380933 1104 106-107 -4.030537393185792 1104 108-109 -3.8504113909955677 1104 110-111 -4.138291747778904 1104 112-113 -3.077703071270303 1104 114-115 -2.4750929575506078 1104 116-117 -2.5708766743223137 1104 118-119 -2.2603956279509774 1104 120-121 -2.303086853989388 1104 122-123 -2.1258785895041363 1104 124-125 -1.247424347732803 1105 1 -0.09700906212537674 1105 2 0.5969042925742691 1105 3 -0.13963537866379383 1105 4 0.18058897748060332 1105 5 -0.42183796647590555 1105 6 0.07804759953782536 1105 7 0.8689103114838801 1105 8 0.76320653598858 1105 9 0.6142397958339174 1105 10-11 0.6281079016283329 1105 12-13 0.3808401375311803 1105 14-15 0.42286888053554605 1105 16-17 0.9343066565879212 1105 18-19 0.7142582349477067 1105 20-21 0.10201205977800498 1105 22-23 0.29600538411644806 1105 24-25 0.58867641029331 1105 26-27 0.6749348768362324 1105 28-29 0.23376873850612867 1105 30-31 0.6939199334827038 1105 32-33 -0.7010639732845547 1105 34-35 -0.9853225672530357 1105 36-37 -0.11344605893033588 1105 38-39 -0.8101931544967584 1105 40-41 -0.8396155116990052 1105 42-43 -0.9943113682528661 1105 44-45 -2.7961597331255668 1105 46-47 -4.998435888314599 1105 48-49 -6.385931091354454 1105 50-51 -5.12534787398079 1105 52-53 -4.378824155552511 1105 54-55 -4.084007749347361 1105 56-57 -4.9358947452104225 1105 58-59 -4.273624077819925 1105 60-61 -4.657190385184666 1105 62-63 -4.111303319369288 1105 64-65 -4.7318472101470235 1105 66-67 -4.7455003251713705 1105 68-69 -4.015659670362098 1105 70-71 -5.090884054799563 1105 72-73 -4.925151174497305 1105 74-75 -4.58031889288144 1105 76-77 -3.9332801524389964 1105 78-79 -4.2131638312389335 1105 80-81 -4.31316096681088 1105 82-83 -4.1869822734589235 1105 84-85 -4.2383221411146685 1105 86-87 -3.70388202958058 1105 88-89 -3.0393934546547037 1105 90-91 -2.8616729073004272 1105 92-93 -3.4950024254144836 1105 94-95 -3.211741718446003 1105 96-97 -3.893118073737039 1105 98-99 -5.152700959668774 1105 100-101 -5.082897108743358 1105 102-103 -4.604993899596373 1105 104-105 -4.38806937307324 1105 106-107 -4.2468835470319455 1105 108-109 -4.449770365354542 1105 110-111 -4.869060978548134 1105 112-113 -5.074497943065175 1105 114-115 -5.681823726781378 1105 116-117 -5.657415135860775 1105 118-119 -5.826100756156107 1105 120-121 -8.147638136040673 1105 122-123 -8.316583717709266 1105 124-125 -8.125629475937933 1106 1 -1.4111116262279388 1106 2 -0.056941861271884164 1106 3 -1.6684815325099471 1106 4 -1.611077689186061 1106 5 -0.24876104339898575 1106 6 -0.5725934261032037 1106 7 -0.5990384064648424 1106 8 -0.7848703870883398 1106 9 -0.5812730246789002 1106 10-11 -0.6250972265768056 1106 12-13 -1.0935188368277977 1106 14-15 -0.17328496561830065 1106 16-17 -1.4022318049505387 1106 18-19 -1.6367033035138334 1106 20-21 -1.798628965863017 1106 22-23 -2.5453407697297123 1106 24-25 -3.885682564065661 1106 26-27 -2.8234625590612126 1106 28-29 -3.3944363896990026 1106 30-31 -2.1490287844660187 1106 32-33 -1.4831152553358393 1106 34-35 -0.9436559005863643 1106 36-37 -1.5733819563662266 1106 38-39 0.41096069165708826 1106 40-41 0.22769218060868823 1106 42-43 1.419150170208674 1106 44-45 0.9009556514898165 1106 46-47 -1.2708717857504936 1106 48-49 0.7070176265942649 1106 50-51 -1.1285530021859138 1106 52-53 -0.8451703093986644 1106 54-55 -1.9461872365268462 1106 56-57 -2.2852537195693934 1106 58-59 -2.3441368983327457 1106 60-61 -0.9761006415949254 1106 62-63 -2.1754058834718535 1106 64-65 -4.319988235788053 1106 66-67 -3.0596028892739398 1106 68-69 -3.6054032601056853 1106 70-71 -3.2559481573636653 1106 72-73 -4.181561430907561 1106 74-75 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2.9819725776494046 2215 92-93 2.7775728254216396 2215 94-95 3.1870877129921276 2215 96-97 2.9003601871325237 2215 98-99 3.488603388157312 2215 100-101 3.672119613664666 2215 102-103 3.6224308495675075 2215 104-105 3.5717968476625437 2215 106-107 3.2765278242055125 2215 108-109 3.6079219423377644 2215 110-111 3.3943169478732678 2215 112-113 3.670485334526802 2215 114-115 3.2287113902754783 2215 116-117 1.706297238721163 2215 118-119 2.3528290097301827 2215 120-121 2.4568769141265534 2215 122-123 1.8840851786118051 2215 124-125 2.4989524638613965 2216 1 -0.14596011107642326 2216 2 0.8521490478190259 2216 3 -0.2025724416008572 2216 4 -0.8648655679739434 2216 5 -1.3623974070353526 2216 6 0.20392172541195208 2216 7 -0.5611596185860535 2216 8 -0.9780522052701599 2216 9 -0.357788176194056 2216 10-11 -1.3561578326374075 2216 12-13 -0.4268521701611263 2216 14-15 -2.127830420163754 2216 16-17 -1.0726863504050854 2216 18-19 -0.23329421260473993 2216 20-21 0.20515891292485833 2216 22-23 0.8012501393611942 2216 24-25 0.19007501169190988 2216 26-27 -0.16247771057635418 2216 28-29 0.2495030042403954 2216 30-31 0.013850003412770207 2216 32-33 0.29718777496719184 2216 34-35 0.2646774327469643 2216 36-37 1.8830574375731715 2216 38-39 1.7859606916570883 2216 40-41 2.6026921806086882 2216 42-43 4.271422897481401 2216 44-45 2.42936474239891 2216 46-47 1.475340335461624 2216 48-49 2.566866111442753 2216 50-51 3.3676591190262037 2216 52-53 2.3764205996922456 2216 54-55 1.1257824604428492 2216 56-57 1.1655038561881774 2216 58-59 1.5270752228793754 2216 60-61 0.647005419011137 2216 62-63 -1.5238907319566977 2216 64-65 -2.12870035700017 2216 66-67 -2.362633192304237 2216 68-69 -2.438736593439021 2216 70-71 -2.2919330058485095 2216 72-73 -2.5073190066651385 2216 74-75 -1.8670321795947267 2216 76-77 -1.92453889369774 2216 78-79 -1.6677092857843867 2216 80-81 -1.737986141636057 2216 82-83 -1.8985207349973834 2216 84-85 -0.608951511744035 2216 86-87 -0.7248610505595998 2216 88-89 -1.3016312168924635 2216 90-91 -0.5190155646430874 2216 92-93 -0.48626116667322705 2216 94-95 -1.1785249352292197 2216 96-97 -1.0294817101006757 2216 98-99 -1.2890645960324072 2216 100-101 -2.0986313744776233 2216 102-103 -3.1819169765194495 2216 104-105 -2.5576497926536597 2216 106-107 -2.343037393185792 2216 108-109 -2.005714421298599 2216 110-111 -1.4792008386879942 2216 112-113 -1.581490950058182 2216 114-115 -2.3898656848233344 2216 116-117 -1.9913312197768605 2216 118-119 -1.7281986582540085 2216 120-121 -1.0095262479287825 2216 122-123 -0.8777725288980776 2216 124-125 -1.573181923490381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 15.0 6 51.0 7 100.0 8 140.0 9 242.0 10 429.0 11 593.0 12 741.0 13 908.0 14 1162.0 15 972.0 16 295.0 17 192.0 18 150.0 19 107.0 20 123.0 21 113.0 22 142.0 23 133.0 24 131.0 25 163.0 26 230.0 27 235.0 28 275.0 29 309.0 30 362.0 31 432.0 32 509.0 33 617.0 34 765.0 35 1244.0 36 1556.0 37 1872.0 38 599.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.100238963652366 15.45088668092064 16.035718777512262 15.413155577914727 2 21.116138763197586 25.314228255404725 33.1385118149824 20.431121166415284 3 18.53318250377074 28.676470588235293 37.889643036701855 14.900703871292107 4 11.720713926596279 20.946455505279037 31.554801407742584 35.778029160382104 5 7.433494748757939 42.99100685491479 34.708508898811395 14.866989497515878 6 24.61035696329814 39.498491704374054 14.91955756661639 20.971593765711415 7 15.943941679235795 40.17094017094017 23.592257415786825 20.292860734037205 8 29.273504273504276 31.78733031674208 18.815987933634993 20.123177476118652 9 22.366767219708397 10.030165912518854 22.12166918049271 45.48139768728004 10-11 24.431246857717444 26.429738562091504 25.99924585218703 23.13976872800402 12-13 29.02840623428859 27.724358974358974 29.179235796882857 14.067998994469583 14-15 23.089492207139266 26.954499748617394 35.35696329813977 14.599044746103571 16-17 13.907742584213173 26.310331825037707 37.86136249371543 21.920563097033686 18-19 13.687782805429865 17.200854700854702 51.35432378079437 17.757038712921066 20-21 14.504776269482154 16.635243841126194 55.401583710407245 13.458396178984414 22-23 13.66892911010558 16.70751633986928 56.451106083459024 13.172448466566115 24-25 13.420688788335847 17.00603318250377 55.75351935646054 13.81975867269985 26-27 13.800904977375566 16.78607340372046 55.913775766716945 13.499245852187029 28-29 13.386123680241328 16.333584715937658 56.721342383107086 13.558949220713925 30-31 13.986299648064355 16.33044243338361 56.290849673202615 13.392408245349422 32-33 13.1315987933635 16.575540472599297 55.58383609854198 14.709024635495224 34-35 13.62179487179487 16.63838612368024 55.68753142282554 14.052287581699346 36-37 14.718451483157367 17.26684263448969 53.296254399195576 14.718451483157367 38-39 14.966691804927098 16.610105580693816 52.59238310708899 15.830819507290094 40-41 15.142659627953744 16.874057315233788 51.4831573655103 16.500125691302163 42-43 15.774258421317244 17.863876319758674 50.35822021116139 16.003645047762692 44-45 17.752427642123124 17.77128311492411 46.84327959523585 17.633009647716914 46-47 17.533549137307897 18.624092523335115 44.57085389232848 19.271504447028505 48-49 18.67634580937117 18.58521102416643 40.872379874925365 21.866063291537035 50-51 18.189817724701445 20.864236329352607 38.53236957888121 22.41357636706474 52-53 18.933442272641567 20.84407014015461 38.03343598768148 22.189051599522344 54-55 18.760605870152723 21.906228395449688 36.8738608509836 22.459304883413992 56-57 17.949846018477782 21.73024951291559 35.67971843378794 24.64018603481868 58-59 18.719165383527635 20.573170348490084 35.12868051409358 25.578983753888696 60-61 18.53318250377074 21.832579185520363 34.91704374057315 24.717194570135746 62-63 17.81045751633987 21.19155354449472 37.207767722473605 23.790221216691805 64-65 17.106586224233283 20.635369532428356 37.51885369532428 24.73919054801408 66-67 18.20067246959746 20.31549508217327 36.70615592495993 24.777676523269335 68-69 19.39416792357969 19.91892911010558 36.30593262946204 24.380970336852688 70-71 19.233911513323278 20.374560080442432 36.84640522875817 23.545123177476118 72-73 19.774384112619405 21.1569884364002 36.34049773755656 22.72812971342383 74-75 18.65887380593263 20.94017094017094 36.96895424836602 23.432001005530417 76-77 18.127828054298643 20.981020613373556 37.2140522875817 23.677099044746104 78-79 18.420060331825038 20.635369532428356 37.09464555052791 23.849924585218705 80-81 18.159250879839114 21.74145299145299 36.54474610356963 23.55455002513826 82-83 18.140397184514832 21.98340874811463 35.875439919557564 24.000754147812973 84-85 17.760180995475114 21.376948215183507 35.375816993464056 25.487053795877323 86-87 17.0280291603821 22.53645047762695 33.826671694318755 26.6088486676722 88-89 17.461664152840623 21.747737556561088 34.14718451483157 26.643413775766717 90-91 17.599924585218705 21.49007038712921 33.52815485168426 27.381850175967827 92-93 17.084590246354953 21.76344896933132 32.00414781297134 29.147812971342386 94-95 16.77664655605832 21.77916038210156 31.13373554550025 30.310457516339866 96-97 17.942433383609853 21.98655103066868 29.729135243841124 30.34188034188034 98-99 17.873303167420815 21.945701357466064 28.421945701357465 31.759049773755656 100-101 18.08383609854198 22.263071895424837 28.36224233283057 31.290849673202615 102-103 18.03984414278532 21.88914027149321 28.616767219708393 31.454248366013072 104-105 18.33521870286576 22.28506787330317 28.092006033182503 31.287707390648567 106-107 17.587355455002516 23.01407742584213 28.00716440422323 31.391402714932127 108-109 18.262946204122674 22.52388134741076 28.447083961789843 30.766088486676722 110-111 18.250377073906485 22.413901458019104 29.11953242835596 30.21618903971845 112-113 17.33924193852536 22.94298824564712 29.31045320258973 30.40731661323779 114-115 18.469856038222165 22.49638523920287 29.543597158483685 29.490161564091284 116-117 18.132956152758133 22.76599088480277 29.97328304258997 29.12776991984913 118-119 18.01552629097652 21.43822484835151 31.294590941949274 29.251657918722696 120-121 17.59733526066053 23.250479213147724 30.553373346321845 28.598812179869903 122-123 17.10137640625982 23.08465841241908 31.732763496951794 28.081201684369304 124-125 16.63943183960782 22.726415687260385 31.283388850480797 29.350763622650994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16.0 1 12.5 2 20.0 3 35.0 4 23.5 5 16.5 6 37.0 7 109.5 8 167.0 9 172.0 10 117.0 11 58.0 12 58.0 13 46.5 14 45.5 15 46.5 16 46.0 17 52.5 18 49.5 19 57.0 20 69.5 21 72.5 22 88.5 23 106.0 24 131.0 25 189.5 26 220.0 27 246.0 28 300.0 29 324.5 30 357.0 31 413.0 32 478.5 33 518.0 34 581.0 35 611.5 36 572.0 37 546.5 38 506.0 39 425.0 40 372.0 41 405.5 42 378.5 43 372.0 44 432.0 45 420.0 46 437.5 47 475.5 48 413.0 49 376.0 50 432.5 51 418.5 52 345.5 53 294.0 54 268.0 55 267.0 56 234.5 57 227.0 58 207.5 59 167.5 60 167.5 61 147.0 62 121.0 63 104.0 64 72.0 65 47.5 66 57.0 67 54.5 68 37.0 69 32.0 70 41.5 71 36.5 72 25.0 73 24.5 74 20.0 75 14.5 76 7.0 77 6.0 78 7.0 79 5.5 80 5.0 81 2.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0628456510809452 2 0.0 3 0.0 4 0.0 5 0.06913021618903972 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.009426847662141781 46-47 0.0157114127702363 48-49 0.009426847662141781 50-51 0.012569130216189038 52-53 0.006284565108094519 54-55 0.006284565108094519 56-57 0.006284565108094519 58-59 0.0031422825540472596 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0031422825540472596 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.018853695324283562 114-115 0.0314228255404726 116-117 0.028280542986425343 118-119 0.02199597787833082 120-121 0.0031422825540472596 122-123 0.006284565108094519 124-125 0.006284565108094519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 15912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.34414278531926 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08008164245408 40.346907993966816 2 9.100732380838036 9.527400703871292 3 5.042622163525033 7.918552036199094 4 3.3257293792772242 6.963298139768728 5 1.9570176491775721 5.121920563097033 6 1.0805618921839355 3.3936651583710407 7 0.6363308920638732 2.331573655103067 8 0.5162684595989915 2.1618903971845147 9 0.20410613519029897 0.9615384615384616 >10 0.9244807299795894 8.779537456008043 >50 0.08404370272541722 2.696078431372549 >100 0.03601872973946452 6.177727501256913 >500 0.012006243246488173 3.619909502262444 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 576 3.619909502262444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 3.104575163398693 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 2.331573655103067 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.7415786827551533 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.5027652086475616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 65 0.40849673202614384 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 62 0.38964303670186023 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.37078934137757663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 58 0.36450477626948213 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.33308195072900953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 52 0.32679738562091504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 49 0.3079436902966315 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.301659125188537 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 41 0.2576671694318753 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 40 0.2513826043237808 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 35 0.2199597787833082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 35 0.2199597787833082 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 34 0.2136752136752137 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 34 0.2136752136752137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 31 0.19482151835093012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 31 0.19482151835093012 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.19482151835093012 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 27 0.16968325791855204 No Hit GTACATGGGCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT 27 0.16968325791855204 No Hit GATTACAGGCGTGCACCACCATGCCCAGATAATTTTGTATTTTTAGTAAA 26 0.16339869281045752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 24 0.1508295625942685 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 24 0.1508295625942685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 24 0.1508295625942685 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.1508295625942685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 23 0.14454499748617397 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 23 0.14454499748617397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 22 0.13826043237807945 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 21 0.13197586726998492 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 20 0.1256913021618904 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.1256913021618904 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20 0.1256913021618904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 18 0.11312217194570137 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17 0.10683760683760685 No Hit GGTATCAACGCAGAGTACATGGAGCCCAACTTAGAGACTTTTTCATTGGC 17 0.10683760683760685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 17 0.10683760683760685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 16 0.10055304172951231 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16 0.10055304172951231 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 16 0.10055304172951231 No Hit GGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAAGCTCTGCCTCCCA 16 0.10055304172951231 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.00314228255404726 0.0 16-17 0.0 0.0 0.0 0.00628456510809452 0.0 18-19 0.0 0.0 0.0 0.01256913021618904 0.0 20-21 0.0 0.0 0.0 0.01885369532428356 0.0 22-23 0.0 0.0 0.0 0.021995977878330818 0.0 24-25 0.0 0.0 0.0 0.04399195575666164 0.0 26-27 0.0 0.0 0.0 0.04399195575666164 0.0 28-29 0.0 0.0 0.0 0.056561085972850686 0.0 30-31 0.0 0.0 0.0 0.10369532428355957 0.0 32-33 0.0 0.0 0.0 0.15082956259426847 0.0 34-35 0.0 0.0 0.0 0.2199597787833082 0.0 36-37 0.0 0.0 0.0 0.30480140774258424 0.0 38-39 0.0 0.0 0.0 0.3959276018099548 0.0 40-41 0.0 0.0 0.0 0.5341880341880342 0.0 42-43 0.0 0.0 0.0 0.6755907491201609 0.0 44-45 0.0 0.0 0.0 0.8107088989441931 0.0 46-47 0.0 0.0 0.0 0.9332579185520362 0.0 48-49 0.0 0.0 0.0 1.0620915032679736 0.0 50-51 0.0 0.0 0.0 1.194067370537959 0.0 52-53 0.0 0.0 0.0 1.3229009552538966 0.0 54-55 0.0 0.0 0.0 1.4548768225238815 0.0 56-57 0.0 0.0 0.0 1.6339869281045751 0.0 58-59 0.0 0.0 0.0 1.80052790346908 0.0 60-61 0.0 0.0 0.0 1.957642031171443 0.0 62-63 0.0 0.0 0.0 2.0896178984414275 0.0 64-65 0.0 0.0 0.0 2.246732026143791 0.0 66-67 0.0 0.0 0.0 2.441553544494721 0.0 68-69 0.0 0.0 0.0 2.633232780291604 0.0 70-71 0.0 0.0 0.0 2.922322775263952 0.0 72-73 0.0 0.0 0.0 3.201985922574158 0.0 74-75 0.0 0.0 0.0 3.4722222222222223 0.0 76-77 0.0 0.0 0.0 3.711035696329814 0.0 78-79 0.0 0.0 0.0 3.965560583207642 0.0 80-81 0.0 0.0 0.0 4.282931121166415 0.0 82-83 0.0 0.0 0.0 4.600301659125188 0.0 84-85 0.0 0.0 0.0 4.939668174962293 0.0 86-87 0.0 0.0 0.0 5.341880341880342 0.0 88-89 0.0 0.0 0.0 5.674962292609351 0.0 90-91 0.0 0.0 0.0 6.086601307189543 0.0 92-93 0.0 0.0 0.0 6.429110105580694 0.0 94-95 0.0 0.0 0.0 6.979009552538964 0.0 96-97 0.0 0.0 0.0 7.415786827551534 0.0 98-99 0.0 0.0 0.0 7.796003016591252 0.0 100-101 0.0 0.0 0.0 8.40560583207642 0.0 102-103 0.0 0.0 0.0 8.854952237305179 0.0 104-105 0.0 0.0 0.0 9.313725490196077 0.0 106-107 0.0 0.0 0.0 9.891905480140775 0.0 108-109 0.0 0.0 0.0 10.39152840623429 0.0 110-111 0.0 0.0 0.0 10.994846656611362 0.0 112-113 0.0 0.0 0.0 11.56988436400201 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 340 0.0 70.0 1 AAATTTC 15 0.0041988236 59.5 76-77 ATTCTTC 15 0.0041988236 59.5 62-63 GTATCAA 500 0.0 47.6 2 CAACGCA 685 0.0 36.48175 6 TCAACGC 670 0.0 36.410446 5 ATCAACG 670 0.0 36.410446 4 AACGCAG 690 0.0 36.217392 7 TATCAAC 690 0.0 35.355076 3 ACGCAGA 745 0.0 33.543625 8 CGCAGAG 750 0.0 33.320004 9 GAGTACT 600 0.0 31.237497 12-13 GTACTTT 655 0.0 28.614504 14-15 CAGAGTA 760 0.0 28.18421 10-11 AGTACTT 615 0.0 27.089432 12-13 TTTGGGG 45 0.009392939 26.444443 34-35 GCAGAGT 785 0.0 25.7707 8 AGAGTAC 755 0.0 25.218544 10-11 ACTTTTT 745 0.0 25.157719 16-17 TACTTTT 705 0.0 23.631207 14-15 >>END_MODULE