FastQCFastQC Report
Thu 26 May 2016
SRR936270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608422
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86470.5376076676394628No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18640.11588998409621355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.071.427181
GTACATG162150.049.824161
GTATCAA46150.049.759132
TACATGG162450.048.5236362
ACATGGG165450.045.6661223
CATGGGA122500.044.8252564
TCAACGC56100.041.0398035
CAACGCA57700.039.9030276
ATCAACG58000.039.797974
AACGCAG58200.039.1513257
ATGGGAG40550.037.9984665
ATGGGAT53350.037.802675
TATCAAC64450.035.907393
CATGGGG35700.035.661764
TGGGATA15800.033.8887946
ATGGGAC21100.033.8342065
CGCAGAG69500.032.3577049
ACGCAGA70450.032.0058178
AGTACAT94350.030.3291572
GAGTACA98450.029.9725111
Produced by FastQC (version 0.11.5)