##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1608422 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.526541541958515 31.0 31.0 34.0 30.0 34.0 2 31.390520646944644 31.0 31.0 34.0 30.0 34.0 3 31.73399083076456 31.0 31.0 34.0 30.0 34.0 4 35.5638644584568 37.0 35.0 37.0 33.0 37.0 5 35.2162604092707 37.0 35.0 37.0 33.0 37.0 6 35.42027962810755 37.0 35.0 37.0 33.0 37.0 7 35.24602747288958 37.0 35.0 37.0 32.0 37.0 8 35.3308690132316 37.0 35.0 37.0 32.0 37.0 9 36.98559706345723 39.0 37.0 39.0 33.0 39.0 10-11 36.87913215561588 39.0 37.0 39.0 32.0 39.0 12-13 36.83095356815562 39.0 37.0 39.0 32.0 39.0 14-15 37.88843568416746 40.0 37.0 41.0 32.5 41.0 16-17 37.81880190646484 40.0 37.0 41.0 32.0 41.0 18-19 38.016385936029224 40.0 37.0 41.0 33.0 41.0 20-21 38.090526615527516 40.0 37.0 41.0 33.0 41.0 22-23 37.96352076755976 40.0 37.0 41.0 33.0 41.0 24-25 37.91222452814 40.0 37.0 41.0 33.0 41.0 26-27 37.714545996013484 40.0 37.0 41.0 32.0 41.0 28-29 37.53951574897633 40.0 36.0 41.0 32.0 41.0 30-31 37.27381371306784 39.0 36.0 41.0 32.0 41.0 32-33 36.95200855248187 39.0 35.0 41.0 31.0 41.0 34-35 36.68109395419859 39.0 35.0 41.0 30.5 41.0 36-37 36.41504468354698 39.0 35.0 41.0 30.0 41.0 38-39 36.358206055376016 39.0 35.0 41.0 30.0 41.0 40-41 36.32903616090802 39.0 35.0 41.0 30.0 41.0 42-43 36.1606978765523 39.0 35.0 41.0 30.0 41.0 44-45 35.86224977027173 39.0 35.0 41.0 28.5 41.0 46-47 35.387830432560605 39.0 35.0 41.0 26.0 41.0 48-49 34.98437381483218 39.0 35.0 41.0 23.5 41.0 50-51 34.80140970466706 39.0 35.0 41.0 23.0 41.0 52-53 34.691595240552545 39.0 35.0 41.0 23.0 41.0 54-55 34.53666730497345 39.0 34.0 41.0 23.0 41.0 56-57 34.35458418250931 38.5 34.0 41.0 22.0 41.0 58-59 34.165416787385396 38.0 34.0 40.0 21.5 41.0 60-61 33.961146701549715 38.0 34.0 40.0 20.5 41.0 62-63 33.76045030470859 38.0 34.0 40.0 20.0 41.0 64-65 33.522553160799845 37.0 33.0 40.0 20.0 41.0 66-67 33.256414050541466 37.0 33.0 40.0 19.0 41.0 68-69 32.96303457674665 36.0 33.0 39.0 18.0 41.0 70-71 32.653512573192856 36.0 33.0 39.0 17.5 41.0 72-73 32.37171463707908 35.5 32.5 39.0 16.0 40.5 74-75 32.15575203522459 35.0 32.0 38.5 15.5 40.0 76-77 31.845963621487396 35.0 32.0 37.0 13.0 39.5 78-79 31.50594930932305 35.0 32.0 37.0 11.5 39.0 80-81 31.20434686916742 35.0 32.0 36.0 9.0 39.0 82-83 30.9252879530372 35.0 32.0 36.0 7.5 37.5 84-85 30.647340685466872 35.0 31.5 36.0 7.0 37.0 86-87 30.432683400251925 35.0 31.0 35.0 6.0 37.0 88-89 30.253326241496325 35.0 31.0 35.0 2.0 36.0 90-91 30.093804051424314 35.0 31.0 35.0 2.0 36.0 92-93 29.953203823374714 35.0 31.0 35.0 2.0 36.0 94-95 29.815063770577623 34.0 31.0 35.0 2.0 36.0 96-97 29.73416273838582 34.0 31.0 35.0 2.0 35.0 98-99 29.639512515993935 34.0 31.0 35.0 2.0 35.0 100-101 29.55140286566585 34.0 31.0 35.0 2.0 35.0 102-103 28.56127900513671 33.5 29.0 34.5 2.0 35.0 104-105 29.27037400632421 34.0 30.5 35.0 2.0 35.0 106-107 29.402638735356767 34.0 31.0 35.0 2.0 35.0 108-109 29.361493438910934 34.0 31.0 35.0 2.0 35.0 110-111 29.314438623694528 34.0 31.0 35.0 2.0 35.0 112-113 29.225131837291457 34.0 31.0 35.0 2.0 35.0 114-115 29.19210971996155 34.0 31.0 35.0 2.0 35.0 116-117 29.078248121450713 34.0 31.0 35.0 2.0 35.0 118-119 28.949977369123275 34.0 31.0 35.0 2.0 35.0 120-121 28.747901980947788 34.0 31.0 35.0 2.0 35.0 122-123 28.537583420271545 34.0 30.5 35.0 2.0 35.0 124-125 26.582893668452684 32.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10786906305255783 1101 2 0.535752414152828 1101 3 0.2902158112562532 1101 4 0.22446667960481648 1101 5 0.3903319418121356 1101 6 0.2544568128292184 1101 7 0.38259199729228754 1101 8 0.32522397053107 1101 9 0.18355611516815173 1101 10-11 0.23883426987486445 1101 12-13 0.2734186360041093 1101 14-15 0.4038011655980043 1101 16-17 0.44905735610740294 1101 18-19 0.4184127339202064 1101 20-21 0.4122408851296271 1101 22-23 0.36504638719766547 1101 24-25 0.14887222824172142 1101 26-27 0.2658337881329942 1101 28-29 0.32223397793975295 1101 30-31 0.2603162120012925 1101 32-33 0.25637512955162123 1101 34-35 0.2871991254261559 1101 36-37 0.3531397611207865 1101 38-39 0.3324961266413595 1101 40-41 0.35783347265174825 1101 42-43 0.2925504441532709 1101 44-45 0.3055973112134325 1101 46-47 0.14759003347628408 1101 48-49 0.20750905094173078 1101 50-51 -0.008985843898166479 1101 52-53 -0.011575007596448472 1101 54-55 0.05899860898863096 1101 56-57 -0.11684069316679313 1101 58-59 0.038065966150604424 1101 60-61 0.1628982401813559 1101 62-63 0.059235068942349756 1101 64-65 0.02351117150716675 1101 66-67 -0.047900245621690374 1101 68-69 -0.004351558621117135 1101 70-71 0.002446084063315368 1101 72-73 0.09182860076179367 1101 74-75 0.08347273217709272 1101 76-77 0.06306840971873484 1101 78-79 0.034960057420505564 1101 80-81 0.07135479852524895 1101 82-83 0.04185560360209095 1101 84-85 0.08965070528712715 1101 86-87 0.16152077140666776 1101 88-89 0.0681747721688879 1101 90-91 0.11992619134313642 1101 92-93 0.24518471145280785 1101 94-95 0.2503004259280175 1101 96-97 0.165239657565035 1101 98-99 0.17629046774450075 1101 100-101 0.18181882013830375 1101 102-103 0.2112324687973981 1101 104-105 0.17814604502078524 1101 106-107 0.20821422979874527 1101 108-109 0.11177815982652106 1101 110-111 0.20997416303078964 1101 112-113 0.2533582575952593 1101 114-115 0.10743782224970744 1101 116-117 0.15440141163956866 1101 118-119 0.186008722372911 1101 120-121 0.049579716412875285 1101 122-123 0.0011438530888696619 1101 124-125 0.08491331489855014 1102 1 0.2513671978974017 1102 2 0.6249663229731475 1102 3 0.42182943436268516 1102 4 0.22312829765573383 1102 5 0.4789492017476178 1102 6 0.2993690714071846 1102 7 0.5217719564146179 1102 8 0.3634869819547646 1102 9 0.42501822490543617 1102 10-11 0.4240993167119598 1102 12-13 0.3432738451300139 1102 14-15 0.3643098009299379 1102 16-17 0.45353653605711486 1102 18-19 0.44963775101269476 1102 20-21 0.4531184703140454 1102 22-23 0.5021717190679311 1102 24-25 0.47071230657777363 1102 26-27 0.4772279467145424 1102 28-29 0.5385455491167122 1102 30-31 0.43400307077823186 1102 32-33 0.3834091117170928 1102 34-35 0.3198853362649956 1102 36-37 0.3574080592551283 1102 38-39 0.28741611938244205 1102 40-41 0.1484203797221113 1102 42-43 0.19834665907172422 1102 44-45 0.3190673185492017 1102 46-47 0.33111140374852255 1102 48-49 0.03983275523412999 1102 50-51 0.11681617752440587 1102 52-53 0.3156422041240319 1102 54-55 0.2638968391027987 1102 56-57 0.18508332695426333 1102 58-59 0.21594693407068632 1102 60-61 0.21923474074153404 1102 62-63 0.13728376985861246 1102 64-65 0.24616088230342115 1102 66-67 0.18332386175377735 1102 68-69 0.15314774850389057 1102 70-71 0.21334021198114073 1102 72-73 0.2981557924073357 1102 74-75 0.1560081558519144 1102 76-77 0.13152124300885504 1102 78-79 0.15537161339892336 1102 80-81 0.19360437499708638 1102 82-83 0.20800318260199546 1102 84-85 0.2634558272762604 1102 86-87 0.13469556929656434 1102 88-89 0.18632717141419874 1102 90-91 0.14670975037255474 1102 92-93 0.21611517472306474 1102 94-95 0.059497319903165646 1102 96-97 0.06585966108156072 1102 98-99 0.09990835271831244 1102 100-101 0.11299715933695609 1102 102-103 0.20056925215408938 1102 104-105 0.20534415268391015 1102 106-107 0.15974615404315884 1102 108-109 0.198011490634606 1102 110-111 0.20233520650130643 1102 112-113 0.140872611126035 1102 114-115 0.13804572182447572 1102 116-117 0.2111203070423464 1102 118-119 0.2144008336206369 1102 120-121 0.23087518167744392 1102 122-123 0.34495947917259073 1102 124-125 0.25628000316315536 1103 1 0.05935985411007039 1103 2 0.5263379736626028 1103 3 0.27078717824391063 1103 4 0.11659447864879269 1103 5 0.36839138968205987 1103 6 0.25186359779857526 1103 7 0.37109018300734675 1103 8 0.3483858302264693 1103 9 0.25997442905042334 1103 10-11 0.32240896018337395 1103 12-13 0.19049065312546531 1103 14-15 0.20642605800161817 1103 16-17 0.3913190364378636 1103 18-19 0.3226220867813794 1103 20-21 0.32296255455521816 1103 22-23 0.31604457485947535 1103 24-25 0.18996334086973832 1103 26-27 0.14112881676332734 1103 28-29 0.13040786148756212 1103 30-31 -0.06430070436998392 1103 32-33 0.07866569017726732 1103 34-35 -0.11325979155965626 1103 36-37 -0.09843769885537768 1103 38-39 -0.05988661763144876 1103 40-41 -0.040956697769090056 1103 42-43 -0.11269473077076952 1103 44-45 8.616841205935089E-4 1103 46-47 0.06996263756347787 1103 48-49 -0.031222159696135066 1103 50-51 -0.1478263368005841 1103 52-53 -0.308304466790176 1103 54-55 -0.18526531803692592 1103 56-57 -0.21334088854174382 1103 58-59 -0.20862694881714816 1103 60-61 -0.2950252846599213 1103 62-63 -0.4368380425590388 1103 64-65 -0.3290060505336214 1103 66-67 -0.2860770243630313 1103 68-69 -0.3928375499492418 1103 70-71 -0.3317641393805246 1103 72-73 -0.24916874123562138 1103 74-75 -0.3321881311761601 1103 76-77 -0.40120094637870096 1103 78-79 -0.3472768167547571 1103 80-81 -0.349758341868899 1103 82-83 -0.34699069058913423 1103 84-85 -0.3129120159892125 1103 86-87 -0.2961678985750602 1103 88-89 -0.23620327749855718 1103 90-91 -0.2564908610508354 1103 92-93 -0.3319373104419583 1103 94-95 -0.2289922435634466 1103 96-97 -0.28718634796963016 1103 98-99 -0.2293548793026119 1103 100-101 -0.19614051851165826 1103 102-103 -0.12014509044700006 1103 104-105 -0.20331330071531895 1103 106-107 -0.2493814638228109 1103 108-109 -0.265617480438749 1103 110-111 -0.12415873585220183 1103 112-113 -0.09702362294740752 1103 114-115 -0.16285115357285918 1103 116-117 -0.22199757499597794 1103 118-119 -0.2827341671548176 1103 120-121 -0.3375962738204059 1103 122-123 -0.4085905070647975 1103 124-125 -0.25204952274619075 1104 1 0.14847184283960146 1104 2 0.4621267909563045 1104 3 0.25039594519003217 1104 4 0.17774323497111055 1104 5 0.42612990558312447 1104 6 0.19616808520012796 1104 7 0.38369971209577614 1104 8 0.284059633316609 1104 9 0.2538047715525593 1104 10-11 0.21863935811404644 1104 12-13 0.19987701308758687 1104 14-15 0.19042684323915182 1104 16-17 0.33655772555307806 1104 18-19 0.355940870125103 1104 20-21 0.42932436429678233 1104 22-23 0.3241350619376959 1104 24-25 0.38061601183211735 1104 26-27 0.3791356991393542 1104 28-29 0.46137647508140844 1104 30-31 0.3574175801169588 1104 32-33 0.25548293768876107 1104 34-35 0.19272556606984637 1104 36-37 0.2621613911928904 1104 38-39 0.21133373387782228 1104 40-41 0.22162927419330458 1104 42-43 0.2223298656402335 1104 44-45 0.23846024297876056 1104 46-47 0.21313615196334723 1104 48-49 0.08020281547649688 1104 50-51 0.09473700184464207 1104 52-53 0.04673075342275723 1104 54-55 -0.028234670677861118 1104 56-57 0.06021369701451107 1104 58-59 0.06397126361270722 1104 60-61 0.046343107219804835 1104 62-63 0.09355711668251132 1104 64-65 0.08154315383599453 1104 66-67 0.022967757271651124 1104 68-69 -0.0035105447066285933 1104 70-71 -0.08095164948468181 1104 72-73 -0.0512722540188264 1104 74-75 -0.0018056743064889247 1104 76-77 -0.03860319585328398 1104 78-79 -0.0478779853729705 1104 80-81 0.034904121467441485 1104 82-83 0.03800411298600892 1104 84-85 0.0657066588069739 1104 86-87 0.031697871997007354 1104 88-89 -0.02310389876002006 1104 90-91 -0.177680034010951 1104 92-93 -0.1597167048852448 1104 94-95 -0.055762838727307695 1104 96-97 -0.053026436655283504 1104 98-99 -0.12554486059548253 1104 100-101 -0.07109536735343269 1104 102-103 0.012038793707368711 1104 104-105 -0.06596001311052291 1104 106-107 0.08825358548548579 1104 108-109 0.10346633212042988 1104 110-111 0.06324628504897234 1104 112-113 0.11378424790411756 1104 114-115 0.09728164395133376 1104 116-117 0.10206418162657727 1104 118-119 0.07097469448170912 1104 120-121 0.09602043788194337 1104 122-123 0.13632923704014743 1104 124-125 0.20359979940905504 1105 1 0.17987835820165188 1105 2 0.49460170218063837 1105 3 0.26330469924283406 1105 4 0.2106226544554417 1105 5 0.34324276647551244 1105 6 0.24309258728442984 1105 7 0.38144712602198183 1105 8 0.24710270881453056 1105 9 0.29450571334660225 1105 10-11 0.10466452408594762 1105 12-13 0.25177063667875643 1105 14-15 0.23937697447274076 1105 16-17 0.35214441692254184 1105 18-19 0.22751181346307447 1105 20-21 0.28279742281152664 1105 22-23 0.25719743649381854 1105 24-25 0.15227959064946361 1105 26-27 0.19665075240476426 1105 28-29 0.1092412272815011 1105 30-31 0.15359019089355286 1105 32-33 0.09550455263394753 1105 34-35 0.2080212276415665 1105 36-37 0.12430590254073337 1105 38-39 0.09132292640523332 1105 40-41 0.07463915531110388 1105 42-43 0.0398395151693407 1105 44-45 0.09579156921004284 1105 46-47 0.17893281708609265 1105 48-49 0.013340070378461633 1105 50-51 0.04925298146483925 1105 52-53 0.02071407903646616 1105 54-55 -0.07443815994759007 1105 56-57 -0.0651058953893866 1105 58-59 0.01901372154075176 1105 60-61 0.018632747485355594 1105 62-63 -0.015453999575008481 1105 64-65 0.0018596583905718944 1105 66-67 -0.002277726748729947 1105 68-69 0.047124780289514945 1105 70-71 -5.965468136892582E-4 1105 72-73 0.016323083328707355 1105 74-75 0.018556376025451016 1105 76-77 0.051440805999426686 1105 78-79 0.02447263991651738 1105 80-81 -0.03161201254522439 1105 82-83 -0.020174055919348177 1105 84-85 -0.03303581456032845 1105 86-87 0.0627029669483754 1105 88-89 0.12904292847481358 1105 90-91 0.03244965566019431 1105 92-93 0.0646325308720499 1105 94-95 0.17184176216817093 1105 96-97 0.125116221982978 1105 98-99 0.06965970942150079 1105 100-101 0.04525633868331269 1105 102-103 0.11857113892892102 1105 104-105 0.12801790876688912 1105 106-107 0.11488551384737988 1105 108-109 0.06933710562668338 1105 110-111 0.11636887266670115 1105 112-113 0.14156948798440183 1105 114-115 0.11308448477270261 1105 116-117 0.07196537044856299 1105 118-119 0.11126804016643987 1105 120-121 0.18168741333354177 1105 122-123 0.1622995936867433 1105 124-125 0.17100291813686042 1106 1 0.16449757328988568 1106 2 0.5486772055885609 1106 3 0.22493209957273308 1106 4 0.10818886776968384 1106 5 0.27234861441052516 1106 6 0.09434060321919446 1106 7 0.24597515191597807 1106 8 0.1979959272017524 1106 9 0.04817662305996606 1106 10-11 0.12990728030268173 1106 12-13 0.15723654187934244 1106 14-15 0.15294331227735114 1106 16-17 0.10678712956764969 1106 18-19 0.06941138085904441 1106 20-21 0.100329556887651 1106 22-23 -0.005921514052381838 1106 24-25 0.09545829657209737 1106 26-27 0.16213035154577682 1106 28-29 0.007879613886338177 1106 30-31 0.13372464049051302 1106 32-33 0.09216665465380203 1106 34-35 0.11810183515456174 1106 36-37 0.08318673413639033 1106 38-39 0.04605511650201777 1106 40-41 -0.033789620637350026 1106 42-43 0.005313667856775339 1106 44-45 0.010888360470815428 1106 46-47 0.052561866913357846 1106 48-49 0.07045469488438982 1106 50-51 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-0.793625560288703 2216 56-57 -1.366443753274659 2216 58-59 -1.3373966772181518 2216 60-61 -0.9920566992362723 2216 62-63 -0.7116068196323155 2216 64-65 -0.724109480895919 2216 66-67 -0.6293483183948112 2216 68-69 -0.6993131759824038 2216 70-71 -0.6842342927417526 2216 72-73 -0.690111912091222 2216 74-75 -0.6257836932461416 2216 76-77 -0.40227868720140947 2216 78-79 -0.46324468199286173 2216 80-81 -0.4779296842052432 2216 82-83 -0.5318816039329555 2216 84-85 -0.4970266883509282 2216 86-87 -0.3967885684075938 2216 88-89 -0.43643561922728935 2216 90-91 -0.42318290260844904 2216 92-93 -0.33729371923975293 2216 94-95 -0.4971199503145982 2216 96-97 -0.5368081467541259 2216 98-99 -0.6604433652382333 2216 100-101 -0.7992622298681198 2216 102-103 -0.7225196266055285 2216 104-105 -0.5035476262991558 2216 106-107 -0.48363968886303255 2216 108-109 -0.48973520701461126 2216 110-111 -0.4810856427867911 2216 112-113 -0.5094324982538048 2216 114-115 -0.6430010684285641 2216 116-117 -0.5149020173089305 2216 118-119 -0.7028613922444009 2216 120-121 -0.7772965363813249 2216 122-123 -0.830999165531221 2216 124-125 -1.0513791181274073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66.0 3 92.0 4 197.0 5 555.0 6 1338.0 7 2478.0 8 3864.0 9 6456.0 10 10871.0 11 13689.0 12 14909.0 13 19133.0 14 27085.0 15 20035.0 16 8858.0 17 7158.0 18 6547.0 19 6254.0 20 6366.0 21 6540.0 22 7259.0 23 8360.0 24 10202.0 25 12773.0 26 16149.0 27 19872.0 28 25219.0 29 31234.0 30 39226.0 31 48924.0 32 61686.0 33 78447.0 34 105122.0 35 148488.0 36 236043.0 37 410564.0 38 185458.0 39 905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.17526513374587 18.93645375915665 12.221785327212933 20.666495779884553 2 18.583042467816203 22.324980197933623 38.00156426998454 21.090413064265633 3 16.94393635501131 29.46683146587152 33.4773461193642 20.111886059752976 4 10.681469590362225 18.075367083618033 38.05558370600732 33.18757962001242 5 9.826721216012423 42.42966070904195 35.642161766682094 12.10145630826353 6 27.512804475442394 39.217941560112955 18.918977730968614 14.35027623347604 7 24.29884694439643 36.059379938846895 23.480031981656555 16.161741135100115 8 27.091459828328635 33.568180489946045 20.739706370591797 18.600653311133524 9 26.953001140248016 11.835202453087561 21.180200221086256 40.03159618557816 10-11 23.524298971289873 26.783953464948873 29.980968924821966 19.71077863893928 12-13 25.269487733940473 26.897636316837247 30.04062366717192 17.79225228205036 14-15 22.51159832432036 27.15083479335647 29.523097793986903 20.81446908833627 16-17 19.526224935441363 29.007477206689913 30.282236341655466 21.184061516213255 18-19 18.715766136001623 26.290550614204484 35.022369751222 19.97131349857189 20-21 20.570316745232283 25.281300554207785 35.5481024258559 18.600280274704026 22-23 20.09767958906307 25.791769821601545 35.07770348826365 19.032847101071734 24-25 19.36282890932852 26.085287318875274 35.62681311247919 18.92507065931702 26-27 19.309515786279967 25.973127699074 35.504861286403695 19.21249522824234 28-29 19.258658486392253 25.576683233628984 35.74055813710581 19.424100142872952 30-31 19.469019946257884 25.621851727966916 35.71258040489374 19.19654792088146 32-33 19.29997227095874 25.967656498108084 35.39982044513194 19.33255078580124 34-35 19.64534738771427 25.945509223653783 35.157971896626435 19.251171492005504 36-37 19.908021651034367 25.749741050545193 34.61324204717419 19.728995251246253 38-39 19.594173668353207 26.244636047007564 34.64619359844618 19.51499668619305 40-41 19.714726607818097 26.14665181152707 34.62231926695855 19.516302313696283 42-43 19.729902725313956 26.367063576566164 33.981230222767145 19.921803475352736 44-45 19.96958854375385 26.47301410734666 33.53831479743365 20.019082551465843 46-47 20.339554783979885 26.642356644134974 32.57360626717314 20.444482304712007 48-49 20.42516638810959 27.065799160349393 31.973440080383615 20.535594371157405 50-51 20.455748730723805 27.280197052972444 31.559626524553746 20.70442769175001 52-53 20.484193784457474 27.511824428302027 31.363631559083593 20.640350228156905 54-55 20.422055312375647 27.578777855153202 31.439576701154 20.55959013131715 56-57 20.518300786684346 27.630760430445818 31.399726113847727 20.45121266902211 58-59 20.70500166317969 27.112036136076078 31.472613492584085 20.71034870816015 60-61 20.795170063395872 27.36741284971793 31.568426847916747 20.26899023896945 62-63 20.461874868269792 27.194679684979057 31.87547757058097 20.467967876170185 64-65 20.530256305676726 27.10909405169884 31.825792468502705 20.534857174121733 66-67 20.49773048106465 27.51056108924474 31.289890669527516 20.701817760163095 68-69 20.650762509827278 27.36735232502003 31.312439555820966 20.669445609331728 70-71 20.47238954075415 27.284571518102734 31.53746387660597 20.70557506453715 72-73 20.87744217083095 27.3065529621585 31.121821684496727 20.69418318251383 74-75 20.341063746845485 27.75736768446334 31.072973354414206 20.828595214276966 76-77 20.358919566864305 27.451884951012197 31.381136842864432 20.80805863925907 78-79 20.473993553294743 27.65076377820822 30.836159867596646 21.03908280090039 80-81 20.30495813431525 27.50467462583564 30.838914647471817 21.35145259237729 82-83 20.685058663269537 27.532070352402393 30.71815331471876 21.064717669609312 84-85 20.444934782088335 27.40737701172365 30.647833224686032 21.499854981501983 86-87 20.120031248163954 27.57429754581297 30.752302966679295 21.553368239343776 88-89 20.645144242725692 27.127113902014425 30.796132802785376 21.43160905247451 90-91 20.492378130288646 27.41690800411341 30.716526630846346 21.3741872347516 92-93 20.41809509243267 27.53989333591973 30.567254450657604 21.47475712098999 94-95 20.344602904000368 27.16093364718522 30.734294038430694 21.760169410383725 96-97 20.673764421199756 27.257870574162084 30.45574597300665 21.612619031631514 98-99 20.29551829479646 27.32240131385677 30.391766447845974 21.9903139435008 100-101 20.208417785581233 27.334177234078005 30.46352160278139 21.99388337755937 102-103 20.44475171956282 27.10415237972075 30.387004998125473 22.064090902590955 104-105 20.231135168002925 27.004627863737436 30.492794279068857 22.27144268919078 106-107 20.116723751921924 27.33766140910326 30.27148499794518 22.274129841029637 108-109 20.284655027378086 27.488229007282378 29.767912643783927 22.459203321555606 110-111 20.25545119431687 27.252520969479775 29.885153589665315 22.606874246538037 112-113 20.20772248619075 27.23193480621656 29.988375574342697 22.57196713324999 114-115 19.970343828761575 27.451080376370328 29.744225628180388 22.834350166687706 116-117 19.82973290869683 27.47611264205636 29.710304669332928 22.983849779913882 118-119 19.829733547894474 27.260788785781266 29.761466543633926 23.14801112269034 120-121 19.727293076004777 27.559223288897734 29.593830829685636 23.119652805411857 122-123 19.782848953338174 27.53702215500263 29.308800601867468 23.371328289791723 124-125 19.709457657194978 27.35026925327217 29.201094049319266 23.739179040213585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 609.0 1 507.5 2 1419.5 3 2403.0 4 1670.5 5 1293.5 6 2090.0 7 5351.5 8 8211.0 9 8693.0 10 6848.5 11 4024.5 12 3307.5 13 3160.5 14 3296.0 15 3582.5 16 3899.5 17 4221.5 18 4627.0 19 4975.0 20 5516.5 21 6325.5 22 7318.5 23 8558.5 24 9871.0 25 11730.0 26 15096.0 27 18824.5 28 20837.5 29 23245.5 30 27758.5 31 31398.5 32 35607.0 33 41173.0 34 47082.5 35 50806.0 36 54010.5 37 58428.0 38 58189.0 39 56113.0 40 54648.5 41 53966.5 42 53427.0 43 53011.5 44 54242.5 45 54794.5 46 53332.0 47 51432.0 48 50412.5 49 48724.0 50 46442.0 51 43279.5 52 40138.5 53 38341.5 54 35978.0 55 32573.5 56 28883.0 57 25720.5 58 23136.5 59 20716.0 60 17972.0 61 14644.5 62 11937.0 63 9453.0 64 6939.5 65 5229.0 66 4024.0 67 3088.0 68 2291.5 69 1603.5 70 1189.5 71 1029.0 72 978.0 73 942.5 74 730.0 75 549.0 76 370.5 77 221.0 78 132.5 79 45.0 80 28.5 81 23.0 82 13.5 83 7.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0696334668389266 2 2.4869095299616644E-4 3 0.0 4 6.217273824904161E-5 5 0.06652482992647452 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1086369124520805E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.865182147471248E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002393650422588102 44-45 0.009108306153484595 46-47 0.010507192764088031 48-49 0.008331146925371576 50-51 0.007678333173756638 52-53 0.006155101086655119 54-55 0.006341619301402244 56-57 0.005564460073289224 58-59 0.003543846080195372 60-61 0.0022693049460900186 62-63 0.0013056275032298737 64-65 0.001865182147471248 66-67 0.0025801686373352266 68-69 0.0010258501811091865 70-71 0.002859945959455914 72-73 0.002642341375584268 74-75 8.082455972375409E-4 76-77 7.149864898639785E-4 78-79 2.176045838716456E-4 80-81 2.7977732212068726E-4 82-83 3.1086369124520803E-4 84-85 4.0412279861877043E-4 86-87 4.66295536867812E-4 88-89 0.0013678002414789154 90-91 0.0014921457179769984 92-93 4.973819059923329E-4 94-95 0.0012434547649808321 96-97 0.0016164911944750817 98-99 0.001709750301848644 100-101 0.001119109288482749 102-103 0.0011812820267317905 104-105 7.771592281130201E-4 106-107 5.595546442413745E-4 108-109 0.0011812820267317905 110-111 8.082455972375409E-4 112-113 0.010600451871461594 114-115 0.020206139930938522 116-117 0.018745080582086047 118-119 0.011066747408329405 120-121 0.006341619301402244 122-123 0.005875323764534432 124-125 0.005284682751168536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1608422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.92571505615017 #Duplication Level Percentage of deduplicated Percentage of total 1 43.35497807030966 11.240088077164877 2 16.47337580210412 8.541680941164614 3 11.996574211478652 9.33059293970265 4 8.307594573368915 8.615213188447278 5 5.56985861352913 7.220128365869995 6 3.804959983678785 5.9187785002146 7 2.502664092582896 4.541834930189395 8 1.6427280897064707 3.4071120294771085 9 1.1780324852976611 2.7487201086644024 >10 4.6871619122564585 20.576549255005133 >50 0.31272759767735747 5.611363940820937 >100 0.16059796020959533 7.6178343634036 >500 0.005588110539115464 0.9858480625446929 >1k 0.002429613277876289 1.1823121930843963 >5k 0.0 0.0 >10k+ 7.288839833628867E-4 2.461943104246331 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14857 0.9237003721660112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12557 0.7807030741932155 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11671 0.7256180281045645 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3816 0.23725116915834277 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2765 0.17190762125860004 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2610 0.1622708468299986 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2072 0.1288219136520142 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.10600451871461594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.217273824904161E-5 0.0 0.0 0.0 0.0 14-15 6.217273824904161E-5 0.0 0.0 0.0 0.0 16-17 6.217273824904161E-5 0.0 0.0 0.0 0.0 18-19 6.217273824904161E-5 0.0 0.0 3.1086369124520805E-5 0.0 20-21 6.217273824904161E-5 0.0 0.0 1.2434547649808322E-4 0.0 22-23 6.217273824904161E-5 0.0 0.0 1.8651821474712483E-4 0.0 24-25 6.217273824904161E-5 0.0 0.0 4.973819059923329E-4 0.0 26-27 6.217273824904161E-5 0.0 0.0 5.595546442413744E-4 0.0 28-29 6.217273824904161E-5 0.0 0.0 7.460728589884992E-4 0.0 30-31 6.217273824904161E-5 0.0 0.0 0.0027356004829578308 0.0 32-33 6.217273824904161E-5 0.0 0.0 0.0085176651401187 0.0 34-35 6.217273824904161E-5 0.0 0.0 0.0146416798576493 0.0 36-37 6.217273824904161E-5 0.0 0.0 0.026889709292710492 0.0 38-39 6.217273824904161E-5 0.0 0.0 0.04861908131075054 0.0 40-41 6.217273824904161E-5 0.0 0.0 0.07267993101312964 0.0 42-43 6.217273824904161E-5 0.0 0.0 0.10233632715792249 0.0 44-45 6.217273824904161E-5 0.0 0.0 0.13904932909398154 0.0 46-47 6.217273824904161E-5 0.0 0.0 0.1838137006332915 0.0 48-49 6.217273824904161E-5 0.0 0.0 0.23184214093067618 0.0 50-51 6.217273824904161E-5 0.0 0.0 0.2789379901543252 0.0 52-53 6.217273824904161E-5 0.0 0.0 0.3321267677263803 0.0 54-55 6.217273824904161E-5 0.0 0.0 0.3973459701496249 0.0 56-57 6.217273824904161E-5 0.0 0.0 0.4768338160010246 0.0 58-59 6.217273824904161E-5 0.0 0.0 0.5692225050391004 0.0 60-61 6.217273824904161E-5 0.0 0.0 0.6664606676606015 0.0 62-63 6.217273824904161E-5 0.0 0.0 0.7656883579060719 0.0 64-65 6.217273824904161E-5 0.0 0.0 0.8822000693847759 0.0 66-67 6.217273824904161E-5 0.0 0.0 1.0208452756801387 0.0 68-69 6.217273824904161E-5 0.0 0.0 1.190918801160392 0.0 70-71 6.217273824904161E-5 0.0 0.0 1.3678624142171643 0.0 72-73 6.217273824904161E-5 0.0 0.0 1.5471064185891512 6.217273824904161E-5 74-75 6.217273824904161E-5 0.0 0.0 1.7422044712146438 6.217273824904161E-5 76-77 6.217273824904161E-5 0.0 0.0 1.966212847125941 6.217273824904161E-5 78-79 6.217273824904161E-5 0.0 0.0 2.2278046433087835 6.217273824904161E-5 80-81 6.217273824904161E-5 0.0 0.0 2.5174363444419434 6.217273824904161E-5 82-83 6.217273824904161E-5 0.0 0.0 2.811824260051156 6.217273824904161E-5 84-85 6.217273824904161E-5 0.0 0.0 3.11647067747146 6.217273824904161E-5 86-87 6.217273824904161E-5 0.0 0.0 3.4574570603983283 6.217273824904161E-5 88-89 6.217273824904161E-5 0.0 0.0 3.836462072764486 6.217273824904161E-5 90-91 6.217273824904161E-5 0.0 0.0 4.246771058839036 6.217273824904161E-5 92-93 6.217273824904161E-5 0.0 0.0 4.670322838160632 6.217273824904161E-5 94-95 6.217273824904161E-5 0.0 0.0 5.117624603493361 6.217273824904161E-5 96-97 6.217273824904161E-5 0.0 0.0 5.59881051117182 6.217273824904161E-5 98-99 6.217273824904161E-5 0.0 0.0 6.129206141174393 6.217273824904161E-5 100-101 6.217273824904161E-5 0.0 0.0 6.700293828360965 6.217273824904161E-5 102-103 6.217273824904161E-5 0.0 0.0 7.270915220010669 6.217273824904161E-5 104-105 6.217273824904161E-5 0.0 0.0 7.855929600565026 6.217273824904161E-5 106-107 6.217273824904161E-5 0.0 0.0 8.484402725155462 6.217273824904161E-5 108-109 6.217273824904161E-5 0.0 0.0 9.154531584372759 6.217273824904161E-5 110-111 6.217273824904161E-5 0.0 0.0 9.871383256384208 6.217273824904161E-5 112-113 6.217273824904161E-5 0.0 0.0 10.59327092019383 6.217273824904161E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6765 0.0 89.2853 1 GTATCAA 11305 0.0 53.40076 2 GTACATG 14030 0.0 42.160076 1 ACATGGG 14150 0.0 41.359676 3 TACATGG 14320 0.0 41.11813 2 TCAACGC 16050 0.0 38.985916 5 CATGGGA 10250 0.0 38.974335 4 CAACGCA 16225 0.0 38.75785 6 ATCAACG 16235 0.0 38.541664 4 AACGCAG 16630 0.0 37.81396 7 TATCAAC 16755 0.0 37.52317 3 TGGTATC 1765 0.0 34.406105 2 ATGGGAG 3375 0.0 34.222206 5 GGAACGC 235 0.0 32.91129 8 ATGGGAT 4205 0.0 32.84748 5 ACGCAGA 19185 0.0 32.77801 8 CGCAGAG 19270 0.0 32.60255 9 GTGGTAT 1995 0.0 30.754177 1 CATGGGG 3370 0.0 30.563019 4 TACCTGG 880 0.0 29.768026 2 >>END_MODULE