FastQCFastQC Report
Thu 26 May 2016
SRR936269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403001
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54141.343420984067037No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16360.4059543276567552No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.2565750457194895No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9690.24044605348373824No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7870.1952848752236347No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.1620343373837782No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.11910640420247096No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.10347368865089665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG150.004237807459.4939248-49
GGTATCA16050.050.7829741
GTACATG56450.048.6912161
GTACAAA6150.047.401661
TACATGG57200.047.22072
ATGGGAT14700.046.5428625
ACATGGG60150.041.3440743
TACAAGG2750.041.104892
CATGGGA44900.040.148464
ACGCAAC300.001297795939.7316320-21
CCCGTCG300.001308936939.66261398-99
GTCGGGA2850.039.6626132
GAACAAA8150.039.419281
GTATCAA21250.038.356082
TGGGATG7200.038.0100066
GTACATA3250.036.6116451
CGGGAGT3100.036.464024
ATGGGAG13400.036.4067275
CATGGGG12550.036.028194
TGGGATC5150.035.811886
Produced by FastQC (version 0.11.5)