##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936269_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 403001 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.93443440586996 31.0 26.0 33.0 10.0 34.0 2 27.1022975129094 31.0 26.0 34.0 10.0 34.0 3 27.278686653382994 31.0 26.0 34.0 10.0 34.0 4 29.858781988134023 35.0 28.0 37.0 7.0 37.0 5 30.9421738407597 35.0 31.0 37.0 10.0 37.0 6 30.200247642065403 35.0 30.0 37.0 8.0 37.0 7 30.443060935332667 35.0 31.0 37.0 8.0 37.0 8 30.03631504636465 35.0 30.0 37.0 8.0 37.0 9 30.704539194691826 37.0 28.0 39.0 7.0 39.0 10-11 31.000514887059833 37.0 30.0 39.0 7.5 39.0 12-13 31.028089260324414 37.0 29.5 39.0 7.0 39.0 14-15 31.94281155629887 38.0 30.0 41.0 7.5 41.0 16-17 32.05777901295531 38.0 30.0 40.5 7.5 41.0 18-19 32.07447748268615 38.0 30.0 40.5 7.5 41.0 20-21 32.230353026419294 38.0 30.0 41.0 7.5 41.0 22-23 32.24153662149722 38.0 29.5 40.5 7.5 41.0 24-25 32.33227336904871 38.0 30.0 40.0 4.5 41.0 26-27 32.48394172719175 38.0 30.0 40.0 2.0 41.0 28-29 32.407609410398486 38.0 30.0 40.0 2.0 41.0 30-31 32.318924766936064 38.0 30.0 40.0 2.0 41.0 32-33 32.14583710710395 38.0 30.0 40.0 2.0 41.0 34-35 32.05153337088494 38.0 30.0 40.0 2.0 41.0 36-37 31.86092838479309 38.0 30.0 40.0 2.0 41.0 38-39 31.581541981285405 38.0 29.0 40.0 2.0 41.0 40-41 31.632749050250496 37.5 29.0 40.0 2.0 41.0 42-43 31.48106704449865 38.0 29.0 40.0 2.0 41.0 44-45 31.35739117272662 37.0 29.0 40.0 2.0 41.0 46-47 31.319969429356256 37.0 29.0 40.0 2.0 41.0 48-49 31.184660832107117 37.0 29.0 40.0 2.0 41.0 50-51 31.08079136280059 37.0 29.0 40.0 2.0 41.0 52-53 30.90911064736812 37.0 29.0 40.0 2.0 41.0 54-55 30.730015558274047 36.0 29.0 40.0 2.0 41.0 56-57 30.530937888491593 36.0 29.0 40.0 2.0 41.0 58-59 30.269149456204822 35.5 28.5 40.0 2.0 41.0 60-61 30.00050372083444 35.0 28.0 40.0 2.0 41.0 62-63 29.71189525584304 35.0 28.0 39.0 2.0 41.0 64-65 29.38633651033124 35.0 28.0 39.0 2.0 41.0 66-67 29.073909245882767 35.0 27.5 39.0 2.0 40.5 68-69 28.773716442390963 34.5 27.0 38.5 2.0 40.0 70-71 28.430480321388778 34.0 26.0 37.5 2.0 40.0 72-73 28.103554333612074 34.0 26.0 37.0 2.0 40.0 74-75 27.749816997972708 34.0 26.0 36.5 2.0 39.0 76-77 27.43095922838901 34.0 26.0 36.0 2.0 39.0 78-79 27.089793821851558 34.0 25.0 36.0 2.0 38.5 80-81 26.7891667762611 33.5 25.0 35.0 2.0 37.0 82-83 26.50740444812792 33.0 24.0 35.0 2.0 37.0 84-85 26.486444202371707 33.5 25.0 35.0 2.0 36.5 86-87 26.347584745447282 34.0 24.5 35.0 2.0 36.0 88-89 26.163647236607353 34.0 24.0 35.0 2.0 36.0 90-91 25.987959086950156 33.0 24.0 35.0 2.0 36.0 92-93 25.838844072347214 33.0 23.0 35.0 2.0 35.0 94-95 25.717019312607164 33.0 22.5 35.0 2.0 35.0 96-97 25.607751593668503 33.0 20.5 35.0 2.0 35.0 98-99 25.489163798601 33.0 20.0 35.0 2.0 35.0 100-101 25.374155399118116 33.0 19.5 35.0 2.0 35.0 102-103 24.51418730970891 31.5 18.0 34.0 2.0 35.0 104-105 25.077252910042407 33.0 18.0 35.0 2.0 35.0 106-107 25.177956134103887 33.0 18.0 35.0 2.0 35.0 108-109 25.11605058051965 33.0 17.5 35.0 2.0 35.0 110-111 24.99223326989263 33.0 15.5 35.0 2.0 35.0 112-113 24.949724690509452 33.0 12.5 35.0 2.0 35.0 114-115 24.83823365202568 33.0 7.5 35.0 2.0 35.0 116-117 24.715693013168703 33.0 2.0 35.0 2.0 35.0 118-119 24.52440068386927 33.0 2.0 35.0 2.0 35.0 120-121 24.328086034525967 33.0 2.0 35.0 2.0 35.0 122-123 24.04543164905298 33.0 2.0 35.0 2.0 35.0 124-125 22.337784521626496 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5989238354152242 1101 2 0.5338680214779821 1101 3 0.7510058878186534 1101 4 0.5724082072062124 1101 5 0.23768972734223226 1101 6 0.5116889154950215 1101 7 0.6603970300246438 1101 8 0.4559276443235305 1101 9 0.42564409135074754 1101 10-11 0.46579192926455804 1101 12-13 0.43780663758391825 1101 14-15 0.2883720046520324 1101 16-17 0.4642889728636206 1101 18-19 0.2384887068064785 1101 20-21 0.30420163076027507 1101 22-23 0.44874894433095847 1101 24-25 0.4704472018569774 1101 26-27 0.4414210120174218 1101 28-29 0.35668273641636716 1101 30-31 0.545635049842943 1101 32-33 0.9482362964024205 1101 34-35 0.5687857874540896 1101 36-37 0.7314845067562352 1101 38-39 0.7144071249131763 1101 40-41 0.5400131649868243 1101 42-43 0.7373654637002076 1101 44-45 0.8341215945333609 1101 46-47 0.6916470439463005 1101 48-49 0.8217052209922961 1101 50-51 0.5646530119753059 1101 52-53 0.41283428463143323 1101 54-55 0.5618845310628515 1101 56-57 0.6161162775505247 1101 58-59 0.7291471880801588 1101 60-61 0.7849820406425785 1101 62-63 0.6637128382076867 1101 64-65 0.7937449661091591 1101 66-67 0.8675266102656849 1101 68-69 0.7983490201285228 1101 70-71 0.7052354676557506 1101 72-73 0.5788832092574125 1101 74-75 0.7169031939254751 1101 76-77 0.9161133970820927 1101 78-79 0.8558473661379224 1101 80-81 0.8599935273057362 1101 82-83 0.5822414696823834 1101 84-85 0.6941504046499318 1101 86-87 0.768639147348555 1101 88-89 0.9001267777316961 1101 90-91 0.9372207816213347 1101 92-93 0.7224548423868349 1101 94-95 0.853196321016199 1101 96-97 0.9255079582086054 1101 98-99 0.9899398942108562 1101 100-101 0.9925429851095764 1101 102-103 0.8742616008827504 1101 104-105 0.9153068915072851 1101 106-107 0.8753144138254463 1101 108-109 0.8890849836602506 1101 110-111 0.8799572974611287 1101 112-113 0.7015663799395817 1101 114-115 0.8449592917465445 1101 116-117 0.8599600111454961 1101 118-119 0.7937619599318033 1101 120-121 0.8123912859127387 1101 122-123 0.8244801608919019 1101 124-125 0.4257484318110123 1102 1 0.4577066378254777 1102 2 0.5245327057701168 1102 3 0.16917896285447043 1102 4 0.41495173112228656 1102 5 0.5815703726934665 1102 6 0.42561755001189283 1102 7 0.7164849807204376 1102 8 0.7974456329003416 1102 9 0.8536922375361478 1102 10-11 0.5930834364580235 1102 12-13 0.6551403929078745 1102 14-15 0.7568306593851943 1102 16-17 0.6489638438130001 1102 18-19 0.8020718978406478 1102 20-21 0.43797879027273723 1102 22-23 0.580412470602333 1102 24-25 0.46393333504899203 1102 26-27 0.17897964908586772 1102 28-29 0.07414161686543963 1102 30-31 0.37800050353192916 1102 32-33 0.5029184295159226 1102 34-35 0.5493006160081393 1102 36-37 0.4426834507983166 1102 38-39 0.4988116274549377 1102 40-41 0.4210449320480123 1102 42-43 0.5088808516371657 1102 44-45 0.24019924080708321 1102 46-47 0.015819255373411778 1102 48-49 0.18025004032142888 1102 50-51 0.30330833396396173 1102 52-53 0.22370888472172012 1102 54-55 0.15808735153037645 1102 56-57 0.10491144526726259 1102 58-59 0.3668498417141812 1102 60-61 0.5023967592827923 1102 62-63 0.3059371703830003 1102 64-65 0.2505571704703833 1102 66-67 0.4144080505294774 1102 68-69 0.5464917707222519 1102 70-71 0.4679994571354307 1102 72-73 0.38404130853315976 1102 74-75 0.45537818852792 1102 76-77 0.6318242059301546 1102 78-79 0.39468399688631806 1102 80-81 0.38554604060334796 1102 82-83 0.49202502775025536 1102 84-85 0.5318790646825136 1102 86-87 0.37759246340971586 1102 88-89 0.6190393177226703 1102 90-91 0.6346093466569549 1102 92-93 0.6405138327497504 1102 94-95 0.6997524359546681 1102 96-97 0.9837610642558303 1102 98-99 0.8388267712840083 1102 100-101 0.8829468693860072 1102 102-103 0.9566785766820765 1102 104-105 0.878696751955971 1102 106-107 0.7782443045004364 1102 108-109 0.7412030330822219 1102 110-111 0.8171493915159722 1102 112-113 0.8008691140462325 1102 114-115 0.9197362746117612 1102 116-117 0.8828198808190919 1102 118-119 1.0159302066098554 1102 120-121 0.8870026619946998 1102 122-123 0.8390241166123289 1102 124-125 0.7371615606261024 1103 1 0.798154025357487 1103 2 0.8702833842091344 1103 3 0.8457259949226597 1103 4 1.0397749547587196 1103 5 0.4116500653892601 1103 6 0.3827038933211462 1103 7 0.3983687478725244 1103 8 0.6979157624726398 1103 9 0.8076204949989787 1103 10-11 0.7993623417823379 1103 12-13 0.7145826395921233 1103 14-15 0.7799835852729018 1103 16-17 0.8346554693911088 1103 18-19 0.726669612170042 1103 20-21 0.6801743527082351 1103 22-23 0.5413353387749424 1103 24-25 0.708816375984668 1103 26-27 0.5960524236147862 1103 28-29 0.8405125414535988 1103 30-31 0.6465261886597773 1103 32-33 1.1165904090289729 1103 34-35 0.8144556058793313 1103 36-37 0.7231797623833849 1103 38-39 0.6315897874518761 1103 40-41 0.8610532453148352 1103 42-43 0.9316797470237788 1103 44-45 0.8475932705437295 1103 46-47 0.8397308863025863 1103 48-49 0.6745836412081765 1103 50-51 0.6258821978174964 1103 52-53 0.5091023793515426 1103 54-55 0.7840835102277737 1103 56-57 0.5985236074810771 1103 58-59 0.6484426445273179 1103 60-61 0.5583273673934777 1103 62-63 0.6172522909731448 1103 64-65 0.5847457191842125 1103 66-67 0.37742117975777845 1103 68-69 0.3487841301611567 1103 70-71 0.46098812433720227 1103 72-73 0.5645538644327139 1103 74-75 0.700000842657797 1103 76-77 0.39914828955970805 1103 78-79 0.45979264268381925 1103 80-81 0.6680012254063143 1103 82-83 0.516221889730911 1103 84-85 0.34250619077661426 1103 86-87 0.29798815579973237 1103 88-89 0.16960213544235003 1103 90-91 0.32091252289821526 1103 92-93 0.3349558883244157 1103 94-95 0.4798593617659286 1103 96-97 0.3673538288470475 1103 98-99 0.22240998039611526 1103 100-101 0.12083768848167509 1103 102-103 0.23619784052796788 1103 104-105 0.2766155686054361 1103 106-107 0.2775610877439476 1103 108-109 0.1969253317482753 1103 110-111 0.10397620801255059 1103 112-113 0.2013153549207516 1103 114-115 0.204071499929956 1103 116-117 0.0351127180319466 1103 118-119 0.045017085025936154 1103 120-121 0.2850504776121774 1103 122-123 0.4226727807563506 1103 124-125 0.324438917962798 1104 1 -0.006799535014028635 1104 2 0.07016483579043253 1104 3 -0.03905669896768771 1104 4 0.04641758516197925 1104 5 0.06234861929449664 1104 6 0.19179195226224266 1104 7 0.13258515262229764 1104 8 0.2747077050991251 1104 9 0.20163722894105618 1104 10-11 0.011674231894811982 1104 12-13 0.33583268857906745 1104 14-15 0.556916610315028 1104 16-17 0.393670047907392 1104 18-19 0.23454181501520566 1104 20-21 0.3282741489225174 1104 22-23 0.27431972173531705 1104 24-25 0.22114149257360793 1104 26-27 0.26273296991724493 1104 28-29 0.2594342407129204 1104 30-31 0.2723687641976511 1104 32-33 0.5756348695846825 1104 34-35 0.5474350901447167 1104 36-37 0.3328811379369405 1104 38-39 0.6439026573033466 1104 40-41 0.5610179747109214 1104 42-43 0.670312325304927 1104 44-45 0.462528901691595 1104 46-47 0.4738498070221091 1104 48-49 0.3063830298510446 1104 50-51 0.08821613205741485 1104 52-53 0.25442657498582477 1104 54-55 0.053891383410359595 1104 56-57 0.48780330337009303 1104 58-59 0.33246946978106706 1104 60-61 0.2920318585170456 1104 62-63 0.33896967546191803 1104 64-65 0.3242698599531124 1104 66-67 0.3255660439659529 1104 68-69 0.474888785880875 1104 70-71 0.3719004572917015 1104 72-73 0.25490980361051285 1104 74-75 0.3394128033114825 1104 76-77 0.354974307508531 1104 78-79 0.3471863410025904 1104 80-81 0.434166918865575 1104 82-83 0.39558417761741893 1104 84-85 0.3670388552747923 1104 86-87 0.33029015054833266 1104 88-89 0.461299827177271 1104 90-91 0.2806770916171075 1104 92-93 0.3072488694742397 1104 94-95 0.5218926141075038 1104 96-97 0.4226968354700844 1104 98-99 0.48654516478299215 1104 100-101 0.35750344072527795 1104 102-103 0.26610192408010747 1104 104-105 0.3263936577224982 1104 106-107 0.336739772542316 1104 108-109 0.21457777913629172 1104 110-111 0.38569525805761984 1104 112-113 0.44803077993153195 1104 114-115 0.26854077531499243 1104 116-117 0.2555246003065079 1104 118-119 0.4491912630249182 1104 120-121 0.2795835340059547 1104 122-123 0.23268914239761074 1104 124-125 -0.0032666312788869334 1105 1 0.15301059156825048 1105 2 0.1524433168030903 1105 3 0.16822178204497007 1105 4 -0.09756975661017364 1105 5 0.13196887245905486 1105 6 -0.4727650097630729 1105 7 -0.5003262397827655 1105 8 -0.389849256926194 1105 9 -0.30627416346400693 1105 10-11 0.03857296607202798 1105 12-13 -0.19739515952219477 1105 14-15 -0.02773528841914441 1105 16-17 -3.172938647608703E-4 1105 18-19 -0.05422400776960501 1105 20-21 -0.16302331943191462 1105 22-23 -0.14814863269506162 1105 24-25 -0.0620863555276614 1105 26-27 0.03804942561345115 1105 28-29 0.09962411413064132 1105 30-31 0.2288561059698111 1105 32-33 0.29161588224290824 1105 34-35 0.27686546989155403 1105 36-37 0.34633050502554497 1105 38-39 0.2665292395818284 1105 40-41 -0.07426683541565993 1105 42-43 0.12600852783657146 1105 44-45 0.02186434472956833 1105 46-47 -0.26111854740826956 1105 48-49 -0.05200304609832429 1105 50-51 -0.02412564009448559 1105 52-53 0.11123037245418033 1105 54-55 0.10927113024580137 1105 56-57 -0.06994986118687052 1105 58-59 0.0919631406671435 1105 60-61 0.17256983320058694 1105 62-63 -0.008340451120361081 1105 64-65 0.2514850498265311 1105 66-67 0.3318951578900027 1105 68-69 0.20590144410872213 1105 70-71 0.17886248260815663 1105 72-73 0.14731486690165596 1105 74-75 0.34415963875451894 1105 76-77 0.43804392776169365 1105 78-79 0.3456040625215735 1105 80-81 0.27831248848582746 1105 82-83 0.3797613928072927 1105 84-85 0.16688062742669274 1105 86-87 0.16810660624453178 1105 88-89 0.28092008034182925 1105 90-91 0.19127835743989152 1105 92-93 0.19648937580334902 1105 94-95 0.11999387993028776 1105 96-97 0.3989626582548951 1105 98-99 0.37262111415007837 1105 100-101 0.24437052933287262 1105 102-103 0.16879179749782836 1105 104-105 0.2615202400009764 1105 106-107 0.363638506719532 1105 108-109 0.27391322217426506 1105 110-111 0.2812648783107825 1105 112-113 0.21780926094418973 1105 114-115 0.3476546993656271 1105 116-117 0.3670752332179035 1105 118-119 0.5306786047970711 1105 120-121 0.5113873314743103 1105 122-123 0.6171828132836836 1105 124-125 0.8203093180882028 1106 1 -0.8965633145415879 1106 2 -0.9986057213709358 1106 3 -0.698819481784394 1106 4 -0.4194849365164828 1106 5 -0.8499183995433377 1106 6 -0.6211453546250283 1106 7 -0.9429954314502638 1106 8 -0.9882644866680437 1106 9 -0.7714341355561025 1106 10-11 -0.6428769990430929 1106 12-13 -0.8210832388603784 1106 14-15 -0.6140840873825653 1106 16-17 -0.5219509029547638 1106 18-19 -0.6488953423654387 1106 20-21 -0.5423689787561372 1106 22-23 -0.9071227680483958 1106 24-25 -4.4667534541023635 1106 26-27 -1.8111170749347423 1106 28-29 -1.1802024581807302 1106 30-31 -1.057370595913973 1106 32-33 -5.62336717367257 1106 34-35 -1.7107217423081842 1106 36-37 -1.2076251911769837 1106 38-39 -0.7566442877778847 1106 40-41 -1.1622701743970225 1106 42-43 -0.8071308621763862 1106 44-45 -0.5921668523201653 1106 46-47 -0.7025194245138202 1106 48-49 -0.4040699752321828 1106 50-51 -0.3254512655305426 1106 52-53 -0.20957098307258093 1106 54-55 -0.2012703329257306 1106 56-57 -0.21882482381021262 1106 58-59 -0.20297979763645557 1106 60-61 -0.2757880856671484 1106 62-63 0.009420936301150817 1106 64-65 -0.21557241055420917 1106 66-67 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70-71 -1.4002368132901104 2216 72-73 -1.576753075990041 2216 74-75 -1.6089717610621435 2216 76-77 -1.5231768403138268 2216 78-79 -1.2440372551823913 2216 80-81 -1.3906219481352657 2216 82-83 -1.431804843466626 2216 84-85 -1.7956749595523007 2216 86-87 -1.3655320491357017 2216 88-89 -1.2335520383997611 2216 90-91 -1.207726440594083 2216 92-93 -1.4689696556179186 2216 94-95 -1.4596844469991659 2216 96-97 -1.6120023609567475 2216 98-99 -1.5546976918026019 2216 100-101 -1.45116520236731 2216 102-103 -1.4455320892997747 2216 104-105 -1.3208837993194962 2216 106-107 -1.3296591176685233 2216 108-109 -1.417423010783935 2216 110-111 -1.4030278992827903 2216 112-113 -1.5085979673135022 2216 114-115 -1.6447325663401315 2216 116-117 -1.541973010428606 2216 118-119 -1.369501321477042 2216 120-121 -1.3307468671955078 2216 122-123 -1.4606962509046468 2216 124-125 -1.2392492042733672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25522.0 3 4652.0 4 4922.0 5 4033.0 6 4217.0 7 3228.0 8 2801.0 9 2940.0 10 3634.0 11 4215.0 12 4408.0 13 3945.0 14 3694.0 15 3456.0 16 3043.0 17 2608.0 18 2315.0 19 2020.0 20 1922.0 21 1764.0 22 2118.0 23 3759.0 24 6364.0 25 8361.0 26 9844.0 27 6646.0 28 6800.0 29 7942.0 30 8837.0 31 10671.0 32 13704.0 33 17625.0 34 23878.0 35 34428.0 36 53754.0 37 73654.0 38 25236.0 39 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.94553363391157 21.357763380239753 9.927518790275954 18.769184195572716 2 17.840154242793442 26.31283793340463 35.74283934779318 20.104168476008745 3 15.42601631261461 38.601144910310396 26.92052873317932 19.052310043895673 4 10.052382263975842 22.783679363968645 32.01174196462026 35.152196407435255 5 10.179009220935194 49.98089311060159 28.73428024099495 11.105817427468264 6 24.662840354442565 42.79216573738396 18.266298427539525 14.278695480633946 7 25.451057948322198 42.595106167043426 18.297406680446755 13.656429204187623 8 25.702055856772414 40.315388528393655 17.398478889316245 16.584076725517686 9 26.2101326822054 23.04705044504495 16.1119423910589 34.630874481690746 10-11 21.256546321466725 36.704190017953145 23.803857878601022 18.235405781979114 12-13 22.768346230627692 35.70000856097461 23.80484451905313 17.726800689344564 14-15 21.43311781849854 35.60014739874711 22.424709389970186 20.54202539278416 16-17 20.00129035780133 37.237120616195476 23.18772968989228 19.57385933611091 18-19 19.83108699131613 34.90124408016606 25.789817823585537 19.477851104932277 20-21 23.110947885351 32.70258432974802 25.126524089516643 19.059943695384337 22-23 22.775540413504654 33.237960765613224 24.518697618045284 19.46780120283684 24-25 21.601200025310707 33.22117215871799 25.55897580966348 19.618652006307823 26-27 21.081455307026904 33.074065618842965 25.65323553204266 20.191243542087474 28-29 21.042666729529238 32.368351507647105 26.12535375705188 20.463628005771778 30-31 21.029143005318918 32.611902614297186 26.191359456867385 20.167594923516514 32-33 20.577900856838703 33.19003059606494 25.879605849233112 20.35246269786324 34-35 20.821122737886384 32.91910361615595 26.125256515557187 20.134517130400475 36-37 21.230449703349137 32.45367854510805 25.67636644259663 20.639505308946177 38-39 21.057574798851107 33.000328786158725 25.747057984230672 20.195038430759496 40-41 20.655219047382722 33.14582066775022 26.289532128190174 19.90942815667688 42-43 20.581918611792467 32.98035084846071 25.933604552915735 20.504125986831088 44-45 20.61072869571259 33.137217923840055 25.825570604028847 20.42648277641851 46-47 20.81311485172838 33.378826209355736 25.429938696281702 20.378120242634186 48-49 20.662158808933004 33.30744416873449 25.42952853598015 20.600868486352358 50-51 20.63881606618436 33.36972745353236 25.475222428042 20.516234052241277 52-53 20.620303923612145 33.443384053439736 25.633256905775742 20.303055117172377 54-55 20.12707369455414 33.80753335343286 25.439305002921873 20.626087949091115 56-57 20.36672866074187 33.90707836688003 25.285890289097523 20.44030268328058 58-59 20.563402998777907 33.36695637069709 25.32062853987928 20.749012090645724 60-61 20.397001951634696 33.82692858853263 25.532295025626837 20.24377443420584 62-63 20.564052362997508 33.709020842716214 25.32912980201963 20.397796992266645 64-65 20.090869275539614 34.199180389904136 25.397242904094703 20.312707430461554 66-67 20.259305210918114 33.8454094292804 25.337468982630273 20.557816377171214 68-69 20.52533563937424 33.680066600578904 25.231172743390502 20.563425016656346 70-71 20.243922108697944 33.93954056786953 25.341844552385563 20.474692771046968 72-73 20.617592285865648 33.684077315040554 25.24115975042215 20.457170648671653 74-75 20.421415960605312 33.95384846345582 25.232103841577576 20.392631734361295 76-77 20.184219276522462 33.881185514568315 25.677174576548765 20.25742063236046 78-79 20.72178480946697 33.750784737506855 25.25887032538381 20.268560127642363 80-81 20.28764339365606 34.01063029933077 25.332196854086487 20.36952945292668 82-83 20.370793124582818 33.842099647395415 25.054900608187076 20.732206619834688 84-85 20.41970171252889 33.42626125774038 25.463988258079084 20.690048771651647 86-87 20.139106872341035 33.61257272031355 25.5250937035902 20.723226703755216 88-89 20.56655227432326 33.11025585671428 25.693860282531716 20.629331586430734 90-91 20.464764267990073 33.4939205955335 25.332009925558314 20.709305210918114 92-93 20.002555840778562 33.76774355239536 25.3250322271671 20.90466837965898 94-95 19.87518610421836 33.61439205955335 25.461290322580645 21.04913151364764 96-97 20.14126603448896 33.987719557268825 25.154560128635715 20.7164542796065 98-99 19.717815326856055 33.95632986226996 25.092649228222935 21.233205582651053 100-101 19.6547634113236 34.434452690949605 25.340261390226775 20.57052250750002 102-103 19.399399995533464 34.70546927148341 25.23902163043397 20.65610910254915 104-105 19.188385082841396 35.00182382586557 25.148697639448237 20.661093451844796 106-107 19.003544678205998 35.6395867478415 24.87912486026623 20.47774371368628 108-109 19.160091961096438 35.843611545013424 24.189540107668428 20.806756386221718 110-111 19.097708165837872 36.286340311070084 24.248627777943206 20.367323745148834 112-113 19.000844912103087 36.27055053281572 24.37329482221486 20.355309732866335 114-115 18.69096611782397 36.6733705624945 24.235513939843596 20.40014937983794 116-117 19.01091197836215 36.513005663806844 24.183276571194607 20.292805786636396 118-119 18.94043350413777 36.348341790841076 24.122383652402636 20.58884105261852 120-121 19.131561120499057 35.90616850165881 24.224625867557982 20.737644510284145 122-123 19.35096452091574 35.73925925190696 24.17741527253237 20.732360954644935 124-125 19.393672456575683 35.71563275434243 23.948014888337468 20.94267990074442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4273.0 1 4475.5 2 4290.5 3 3154.0 4 1912.0 5 1201.0 6 876.5 7 823.0 8 891.0 9 919.0 10 785.0 11 590.5 12 555.0 13 566.5 14 557.0 15 558.0 16 591.0 17 644.0 18 763.0 19 896.0 20 1014.5 21 1239.5 22 1563.0 23 1895.5 24 2375.0 25 2893.5 26 3493.0 27 3989.0 28 4441.5 29 5230.0 30 6182.0 31 7175.0 32 8295.5 33 9566.5 34 10673.0 35 11259.5 36 12027.0 37 13325.5 38 13648.0 39 13253.5 40 12728.5 41 12439.0 42 12381.5 43 12321.0 44 12574.5 45 13022.5 46 12823.0 47 12345.0 48 12172.0 49 11646.5 50 11225.0 51 10412.5 52 9832.5 53 9649.5 54 9143.5 55 8728.0 56 8197.0 57 7264.0 58 6706.0 59 6451.5 60 5709.0 61 5278.5 62 4600.0 63 3206.5 64 2218.5 65 1838.0 66 1502.0 67 1132.0 68 844.0 69 639.5 70 603.0 71 502.5 72 423.0 73 464.5 74 381.0 75 228.0 76 150.0 77 128.5 78 133.0 79 103.5 80 48.0 81 17.5 82 14.0 83 11.0 84 9.0 85 6.0 86 5.0 87 4.5 88 2.5 89 1.5 90 2.0 91 3.0 92 2.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 4.962766841769623E-4 5 0.0012406917104424058 6 4.962766841769623E-4 7 9.925533683539246E-4 8 0.001488830052530887 9 0.001985106736707849 10-11 0.0018610375656636087 12-13 0.002357314249840571 14-15 0.0031017292761060145 16-17 0.002977660105061774 18-19 0.0031017292761060145 20-21 0.003598005960282977 22-23 0.09168711740169379 24-25 0.002357314249840571 26-27 0.0017369683946193682 28-29 0.0013647608814866465 30-31 0.0013647608814866465 32-33 0.001985106736707849 34-35 9.925533683539246E-4 36-37 4.962766841769623E-4 38-39 8.684841973096841E-4 40-41 0.001488830052530887 42-43 0.0016128992235751277 44-45 0.0017369683946193682 46-47 0.0018610375656636087 48-49 2.4813834208848115E-4 50-51 0.0013647608814866465 52-53 0.0012406917104424058 54-55 0.0016128992235751277 56-57 0.0013647608814866465 58-59 8.684841973096841E-4 60-61 0.0013647608814866465 62-63 0.0016128992235751277 64-65 0.0011166225393981653 66-67 2.4813834208848115E-4 68-69 3.7220751313272175E-4 70-71 8.684841973096841E-4 72-73 1.2406917104424057E-4 74-75 6.203458552212029E-4 76-77 7.444150262654435E-4 78-79 0.0 80-81 4.962766841769623E-4 82-83 0.0 84-85 1.2406917104424057E-4 86-87 3.7220751313272175E-4 88-89 4.962766841769623E-4 90-91 2.4813834208848115E-4 92-93 6.203458552212029E-4 94-95 2.4813834208848115E-4 96-97 6.203458552212029E-4 98-99 6.203458552212029E-4 100-101 4.962766841769623E-4 102-103 9.925533683539246E-4 104-105 4.962766841769623E-4 106-107 6.203458552212029E-4 108-109 0.0011166225393981653 110-111 0.0012406917104424058 112-113 1.2406917104424057E-4 114-115 3.7220751313272175E-4 116-117 8.684841973096841E-4 118-119 0.001488830052530887 120-121 4.962766841769623E-4 122-123 7.444150262654435E-4 124-125 2.4813834208848115E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 403001.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.793393604839686 #Duplication Level Percentage of deduplicated Percentage of total 1 66.96125643237522 27.985381463528604 2 11.076609388577177 9.258581919677372 3 6.913029650714255 8.667569075826846 4 4.544817023821025 7.5977330695411265 5 2.9148330247059784 6.091038194696117 6 1.975930891161906 4.954851448217471 7 1.2872209586861039 3.76581325279372 8 0.9076002647062412 3.03453560789797 9 0.6416435701191704 2.4134816052004537 >10 2.6370985915015637 18.192038731441585 >50 0.10022573904112332 2.778470642705615 >100 0.03617615432845276 2.6597474100690395 >500 0.001779155130907513 0.5970861532808047 >1k 0.0011861034206050085 0.661776682963781 >5k 5.930517103025042E-4 1.341894742159517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5414 1.343420984067037 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1636 0.4059543276567552 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1034 0.2565750457194895 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 969 0.24044605348373824 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 787 0.1952848752236347 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 653 0.1620343373837782 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 480 0.11910640420247096 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 417 0.10347368865089665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.4813834208848115E-4 0.0 20-21 0.0 0.0 0.0 3.722075131327217E-4 0.0 22-23 0.0 0.0 0.0 6.203458552212029E-4 0.0 24-25 0.0 0.0 0.0 9.925533683539246E-4 0.0 26-27 0.0 0.0 0.0 0.0013647608814866463 0.0 28-29 0.0 0.0 0.0 0.00210917590775209 0.0 30-31 0.0 0.0 0.0 0.007320081091610194 0.0 32-33 0.0 0.0 0.0 0.020223274880211216 0.0 34-35 0.0 0.0 0.0 0.03263019198463527 0.0 36-37 0.0 0.0 0.0 0.055210781114687066 0.0 38-39 0.0 0.0 0.0 0.09143897905960531 0.0 40-41 0.0 0.0 0.0 0.12518579358363874 0.0 42-43 0.0 0.0 0.0 0.17183580189627323 0.0 44-45 0.0 0.0 0.0 0.22506147627425244 0.0 46-47 0.0 0.0 0.0 0.28300177915191277 0.0 48-49 0.0 0.0 0.0 0.34875843980536025 0.0 50-51 0.0 0.0 0.0 0.41724462222178105 0.0 52-53 0.0 0.0 0.0 0.49230647070354666 0.0 54-55 0.0 0.0 0.0 0.5825047580527096 0.0 56-57 0.0 0.0 0.0 0.692554112768951 0.0 58-59 0.0 0.0 0.0 0.8116605169714219 0.0 60-61 0.0 0.0 0.0 0.9430497691072728 0.0 62-63 0.0 0.0 0.0 1.0833720015583088 0.0 64-65 0.0 2.4813834208848115E-4 0.0 1.2266718941144066 0.0 66-67 0.0 2.4813834208848115E-4 0.0 1.4128997198518118 0.0 68-69 0.0 2.4813834208848115E-4 0.0 1.6107900476673755 0.0 70-71 0.0 2.4813834208848115E-4 0.0 1.8174892866270804 0.0 72-73 0.0 2.4813834208848115E-4 0.0 2.029647569112732 0.0 74-75 0.0 2.4813834208848115E-4 0.0 2.2718305909910894 0.0 76-77 0.0 2.4813834208848115E-4 0.0 2.564509765484453 0.0 78-79 0.0 2.4813834208848115E-4 0.0 2.8810102208183106 0.0 80-81 0.0 2.4813834208848115E-4 0.0 3.2307612139920248 0.0 82-83 0.0 2.4813834208848115E-4 0.0 3.584234282297066 0.0 84-85 0.0 2.4813834208848115E-4 0.0 3.956565864600832 0.0 86-87 0.0 2.4813834208848115E-4 0.0 4.375299316875145 0.0 88-89 0.0 2.4813834208848115E-4 0.0 4.814256044029667 0.0 90-91 0.0 2.4813834208848115E-4 0.0 5.2886965541028435 0.0 92-93 0.0 2.4813834208848115E-4 0.0 5.787702760042779 0.0 94-95 0.0 2.4813834208848115E-4 0.0 6.314128252783492 0.0 96-97 0.0 2.4813834208848115E-4 0.0 6.873928352535105 0.0 98-99 0.0 2.4813834208848115E-4 0.0 7.506308917347599 0.0 100-101 0.0 2.4813834208848115E-4 0.0 8.166729114816093 0.0 102-103 0.0 2.4813834208848115E-4 0.0 8.807298244917506 0.0 104-105 0.0 2.4813834208848115E-4 0.0 9.497370974265573 0.0 106-107 0.0 2.4813834208848115E-4 0.0 10.214862990414417 0.0 108-109 0.0 2.4813834208848115E-4 0.0 10.943769370299329 0.0 110-111 0.0 2.4813834208848115E-4 0.0 11.725281078707 0.0 112-113 0.0 2.4813834208848115E-4 0.0 12.491284140734141 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTCG 15 0.0042378074 59.49392 48-49 GGTATCA 1605 0.0 50.782974 1 GTACATG 5645 0.0 48.691216 1 GTACAAA 615 0.0 47.40166 1 TACATGG 5720 0.0 47.2207 2 ATGGGAT 1470 0.0 46.542862 5 ACATGGG 6015 0.0 41.344074 3 TACAAGG 275 0.0 41.10489 2 CATGGGA 4490 0.0 40.14846 4 ACGCAAC 30 0.0012977959 39.73163 20-21 CCCGTCG 30 0.0013089369 39.662613 98-99 GTCGGGA 285 0.0 39.662613 2 GAACAAA 815 0.0 39.41928 1 GTATCAA 2125 0.0 38.35608 2 TGGGATG 720 0.0 38.010006 6 GTACATA 325 0.0 36.611645 1 CGGGAGT 310 0.0 36.46402 4 ATGGGAG 1340 0.0 36.406727 5 CATGGGG 1255 0.0 36.02819 4 TGGGATC 515 0.0 35.81188 6 >>END_MODULE