FastQCFastQC Report
Thu 26 May 2016
SRR936269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403001
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89672.2250565135074107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74411.8463974034803883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65861.634239120994737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.6052094163538055No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.3987583157361892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.3660040545805097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.2940439353748502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10780.2674931327713827No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.24019791514164976No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6860.17022290267269807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6730.16699710422554784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6270.15558274048947768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4280.10620321041386994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.083.876851
GACGTGA150.004237492659.49503724-25
GTTATCA2650.058.4595261
GTATCAA55800.054.4176982
TATAACG350.00777225251.0337372
CACGCAG952.6102498E-950.138415
CCACGCA909.652649E-846.3083884
CTAATGT558.494104E-443.3013534
CATGGGG9700.039.897514
ATCAACG79450.039.8677644
GTATTAC905.097643E-639.72251
CCTAATG600.001303256839.692913
TCAACGC79950.039.6184355
TATCAAC80650.039.4222153
CAACGCA81200.039.1260456
GTACATG45150.038.1388781
AACGCAG83250.038.0911187
TACATGG46450.037.0438652
GGAACGC650.001938201336.612338
ACATGGG46800.036.2579423
Produced by FastQC (version 0.11.5)