##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 403001 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45809811886323 31.0 31.0 34.0 30.0 34.0 2 31.273716442390963 31.0 31.0 34.0 28.0 34.0 3 31.579117669683203 31.0 31.0 34.0 28.0 34.0 4 35.477492611680866 37.0 35.0 37.0 33.0 37.0 5 35.14923039893201 37.0 35.0 37.0 33.0 37.0 6 35.397403480388384 37.0 35.0 37.0 33.0 37.0 7 35.204634727953525 37.0 35.0 37.0 32.0 37.0 8 35.31456745764899 37.0 35.0 37.0 32.0 37.0 9 36.93287361569823 39.0 37.0 39.0 33.0 39.0 10-11 36.83088503502473 39.0 37.0 39.0 32.0 39.0 12-13 36.76566683457361 39.0 37.0 39.0 32.0 39.0 14-15 37.64620931461708 39.0 36.5 41.0 32.0 41.0 16-17 37.60276401299252 39.0 36.5 41.0 32.0 41.0 18-19 37.93839717519312 40.0 37.0 41.0 32.5 41.0 20-21 38.03434730931189 40.0 37.0 41.0 33.0 41.0 22-23 37.85406611894263 40.0 37.0 41.0 32.0 41.0 24-25 37.74457259411267 40.0 37.0 41.0 32.0 41.0 26-27 37.45639464914479 40.0 36.0 41.0 32.0 41.0 28-29 37.21516323780834 39.5 36.0 41.0 32.0 41.0 30-31 36.70510743149521 39.0 35.0 40.5 31.0 41.0 32-33 36.0816362738554 38.0 35.0 40.0 30.0 41.0 34-35 35.601585355867606 38.0 35.0 40.0 30.0 41.0 36-37 35.163203068975015 38.0 35.0 40.0 27.0 41.0 38-39 34.971178731566425 38.0 35.0 40.0 26.0 41.0 40-41 34.740236376584676 38.0 35.0 40.0 24.5 41.0 42-43 34.38629060473795 38.0 34.0 40.5 22.5 41.0 44-45 33.78957248245042 38.0 33.5 41.0 16.5 41.0 46-47 32.843589718139654 38.0 33.0 40.0 4.5 41.0 48-49 32.064167334572375 38.0 31.5 40.0 2.0 41.0 50-51 31.788749903846394 38.0 31.0 40.0 2.0 41.0 52-53 31.6771261113496 37.5 31.0 40.0 2.0 41.0 54-55 31.53348502857313 37.0 31.0 40.0 2.0 41.0 56-57 31.3434284282173 37.0 31.0 40.0 2.0 41.0 58-59 31.138899159059157 36.5 31.0 40.0 2.0 41.0 60-61 30.927745836858968 36.0 31.0 40.0 2.0 41.0 62-63 30.707086334773365 36.0 30.0 40.0 2.0 41.0 64-65 30.461934337631916 35.0 30.0 39.0 2.0 41.0 66-67 30.196513160016973 35.0 29.5 39.0 2.0 41.0 68-69 29.901775677975984 35.0 29.0 39.0 2.0 41.0 70-71 29.58827893727311 35.0 29.0 38.0 2.0 40.0 72-73 29.30537393207461 35.0 29.0 37.0 2.0 40.0 74-75 29.099571713221557 35.0 29.0 37.0 2.0 39.5 76-77 28.80758732608604 35.0 29.0 36.5 2.0 39.0 78-79 28.475817677871767 35.0 28.5 36.0 2.0 39.0 80-81 28.191947414522545 35.0 28.0 36.0 2.0 37.5 82-83 27.94091081659847 34.0 27.5 35.0 2.0 37.0 84-85 27.685555370830343 34.0 27.0 35.0 2.0 37.0 86-87 27.467261867836555 34.0 27.0 35.0 2.0 36.0 88-89 27.260660643521977 34.0 26.5 35.0 2.0 36.0 90-91 27.085447678789876 34.0 26.0 35.0 2.0 36.0 92-93 26.92680167046732 34.0 26.0 35.0 2.0 35.5 94-95 26.77820154292421 34.0 25.0 35.0 2.0 35.0 96-97 26.66668569060623 34.0 25.0 35.0 2.0 35.0 98-99 26.53385103262771 34.0 25.0 35.0 2.0 35.0 100-101 26.412719819553796 34.0 24.5 35.0 2.0 35.0 102-103 25.447599385609465 32.5 21.5 34.5 2.0 35.0 104-105 26.065002320093498 34.0 23.5 35.0 2.0 35.0 106-107 26.148421467936803 34.0 23.5 35.0 2.0 35.0 108-109 26.063621430219776 34.0 23.0 35.0 2.0 35.0 110-111 25.97701246398892 34.0 20.0 35.0 2.0 35.0 112-113 25.848550003598007 34.0 19.5 35.0 2.0 35.0 114-115 25.76944846290704 34.0 19.0 35.0 2.0 35.0 116-117 25.62560514738177 34.0 17.5 35.0 2.0 35.0 118-119 25.456668842012796 34.0 11.0 35.0 2.0 35.0 120-121 25.226403904704952 34.0 2.0 35.0 2.0 35.0 122-123 24.980947938094445 34.0 2.0 35.0 2.0 35.0 124-125 23.183206741422477 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07137211490595519 1101 2 0.6839378386689603 1101 3 0.4044891132612136 1101 4 0.1880411812442233 1101 5 0.3888710391150809 1101 6 0.23947488527211647 1101 7 0.4329768219645871 1101 8 0.2169349369627298 1101 9 0.4270906976797946 1101 10-11 0.3268804512084884 1101 12-13 0.2520868986314113 1101 14-15 0.45031911407996716 1101 16-17 0.6082922629055432 1101 18-19 0.5718326901258237 1101 20-21 0.44234394421896184 1101 22-23 0.19380517379711648 1101 24-25 0.3362292708159629 1101 26-27 0.5115170510199292 1101 28-29 0.34707625761962646 1101 30-31 0.31314538113930723 1101 32-33 0.31064815857829586 1101 34-35 0.3288459311312195 1101 36-37 0.32022161317211584 1101 38-39 0.3078008615075021 1101 40-41 0.2954501742989777 1101 42-43 0.310858887555824 1101 44-45 0.19190814716534277 1101 46-47 0.14720940250953163 1101 48-49 0.1975874128003241 1101 50-51 0.10818914004548219 1101 52-53 0.15180877148772254 1101 54-55 0.25374541053311717 1101 56-57 0.15554813648725485 1101 58-59 0.23674286994576121 1101 60-61 0.2608636011603629 1101 62-63 0.08982663214505493 1101 64-65 0.07584766671757137 1101 66-67 0.09350003893684544 1101 68-69 0.18760269171485788 1101 70-71 -0.11927144863563655 1101 72-73 -0.054795652043850396 1101 74-75 -0.13598561697717315 1101 76-77 0.00792511740501567 1101 78-79 -0.07065140989619678 1101 80-81 -0.09986715911569277 1101 82-83 -0.027558490698059046 1101 84-85 -0.07080755651966442 1101 86-87 0.04212406560873205 1101 88-89 0.10697870826538747 1101 90-91 -0.004980894452454976 1101 92-93 -0.1096337759649586 1101 94-95 0.037382967359036456 1101 96-97 0.1972417565877187 1101 98-99 0.042119575382862706 1101 100-101 0.09590363148362613 1101 102-103 0.25264094614401245 1101 104-105 0.20243632527387234 1101 106-107 0.26378565156285205 1101 108-109 0.09794851584955566 1101 110-111 0.009822326245060964 1101 112-113 0.1396091674021953 1101 114-115 0.27812857533734814 1101 116-117 0.2916270988256642 1101 118-119 0.4120167817314062 1101 120-121 0.35273917912125086 1101 122-123 0.13658698253869872 1101 124-125 0.20406178317379897 1102 1 0.11874792208568508 1102 2 0.6159850819907149 1102 3 0.24301145913650046 1102 4 0.09429547475341593 1102 5 0.41740202107743585 1102 6 0.2248524196692614 1102 7 0.4336466093990836 1102 8 0.4288282027775594 1102 9 0.497833008618187 1102 10-11 0.5025180986598556 1102 12-13 0.32013043236121774 1102 14-15 0.4665817001954977 1102 16-17 0.5530384797105867 1102 18-19 0.3720826950326881 1102 20-21 0.3105368920687823 1102 22-23 0.19196387171071194 1102 24-25 0.3743764866592585 1102 26-27 0.34679227722652683 1102 28-29 0.45929754753699115 1102 30-31 0.479115272599401 1102 32-33 0.37802926517509405 1102 34-35 0.47474918732859095 1102 36-37 0.5013181508863056 1102 38-39 0.5238821388632857 1102 40-41 0.4724396637884709 1102 42-43 0.6395196561675789 1102 44-45 0.8810777088839288 1102 46-47 0.7232118755708257 1102 48-49 0.7021814676394769 1102 50-51 0.6577301025766431 1102 52-53 0.9845704547396075 1102 54-55 0.9585556128923365 1102 56-57 0.8951934190246931 1102 58-59 0.8176166358685428 1102 60-61 0.6919122969149392 1102 62-63 0.8249441025566426 1102 64-65 0.6763317292114408 1102 66-67 0.3047210638833562 1102 68-69 0.4833800867569593 1102 70-71 0.6699221567344367 1102 72-73 0.8173644983025738 1102 74-75 0.8198513474382167 1102 76-77 0.9777061729703824 1102 78-79 0.5499520366876389 1102 80-81 0.39537808612955416 1102 82-83 0.5360161133177215 1102 84-85 0.529427482440866 1102 86-87 0.5917030563641923 1102 88-89 0.5142646706984095 1102 90-91 0.5323297004561773 1102 92-93 0.6047227374111586 1102 94-95 0.4930187502006973 1102 96-97 0.40246045567896616 1102 98-99 0.6104575211717886 1102 100-101 0.7455348803795765 1102 102-103 0.716216040846561 1102 104-105 0.5808367845566842 1102 106-107 0.592421885836341 1102 108-109 0.6431505806594622 1102 110-111 0.625223266400905 1102 112-113 0.7969797733022119 1102 114-115 0.5633574316446328 1102 116-117 0.5522688680458643 1102 118-119 0.6452745493029006 1102 120-121 0.5841679117754737 1102 122-123 0.6720513684932854 1102 124-125 0.5002857031722279 1103 1 0.11438106813162818 1103 2 0.5676295799458408 1103 3 0.14647723141032287 1103 4 0.0016551047652697548 1103 5 0.2659524548961869 1103 6 -0.10288140290460035 1103 7 0.07191916988526259 1103 8 0.06125758778692614 1103 9 0.09519378528752753 1103 10-11 -0.07614021651806269 1103 12-13 0.016227974691993552 1103 14-15 0.0699743730541158 1103 16-17 0.22152546514803362 1103 18-19 0.05342418874518984 1103 20-21 -0.0018866640983290495 1103 22-23 0.05979965124669917 1103 24-25 0.05675474985537221 1103 26-27 0.10173962653662016 1103 28-29 0.20846860972405068 1103 30-31 0.08814328926415271 1103 32-33 0.11102051235622667 1103 34-35 0.3459365511630139 1103 36-37 0.14130353100326687 1103 38-39 0.6006843022237547 1103 40-41 0.6722805650612571 1103 42-43 0.9417475161224758 1103 44-45 0.8455982149420933 1103 46-47 0.7152999388663233 1103 48-49 0.57485709734555 1103 50-51 0.30940661138679815 1103 52-53 0.16482023496358167 1103 54-55 0.3038667392922143 1103 56-57 0.16307888705205897 1103 58-59 -0.005810054495707817 1103 60-61 0.19563893376851027 1103 62-63 0.1913871710120958 1103 64-65 0.32641247300993115 1103 66-67 0.06505053680312756 1103 68-69 0.11495186751604791 1103 70-71 0.17873708621500128 1103 72-73 0.3622824167370027 1103 74-75 0.029649057945423607 1103 76-77 0.012129786470364223 1103 78-79 -0.14242364652911377 1103 80-81 -0.16605408907971153 1103 82-83 -0.24323081937348334 1103 84-85 -0.09576362714136977 1103 86-87 0.013214350940501163 1103 88-89 -0.003974350056090259 1103 90-91 -0.05757063589668476 1103 92-93 -0.1944593135768784 1103 94-95 -0.04610377015247025 1103 96-97 0.10494822466570142 1103 98-99 0.046177813187231465 1103 100-101 -0.10274083341471041 1103 102-103 -0.09645118003789577 1103 104-105 0.15896715951368634 1103 106-107 0.24290873749708197 1103 108-109 0.1519607324004717 1103 110-111 0.11355841527526778 1103 112-113 0.20646549741642062 1103 114-115 0.3681628820899192 1103 116-117 0.4541030422613588 1103 118-119 0.3874581673895072 1103 120-121 0.06998041416434475 1103 122-123 0.1239729753426495 1103 124-125 0.4802311413865006 1104 1 0.11310250873984984 1104 2 0.26645781210479313 1104 3 0.059642964059147374 1104 4 0.04561599358448376 1104 5 0.19795366977254503 1104 6 0.01800369330576501 1104 7 0.2636981799569824 1104 8 0.24749127513261726 1104 9 0.46169454948394417 1104 10-11 0.22877415429355352 1104 12-13 0.14755463926854873 1104 14-15 0.28356482255836113 1104 16-17 0.15943205683201 1104 18-19 0.23707136513739613 1104 20-21 0.21938983784155397 1104 22-23 0.3430323980429506 1104 24-25 -0.03301138644592072 1104 26-27 -0.054999018288398815 1104 28-29 -0.0878289566042838 1104 30-31 0.022559560769423115 1104 32-33 -0.16928281483740903 1104 34-35 -0.21623768562030676 1104 36-37 -0.2543077281572934 1104 38-39 -0.2705583987207092 1104 40-41 -0.35465245998086914 1104 42-43 -0.20476265356832357 1104 44-45 0.05851754323304448 1104 46-47 -0.21727687267108564 1104 48-49 -0.27489502868807136 1104 50-51 -0.5837822431023696 1104 52-53 -0.706045266466834 1104 54-55 -0.6647546699643598 1104 56-57 -0.589882374067976 1104 58-59 -0.5747395916670079 1104 60-61 -0.7530888751431952 1104 62-63 -0.6912302996937107 1104 64-65 -0.6177864923856866 1104 66-67 -0.5870784360385031 1104 68-69 -0.6938937060231609 1104 70-71 -0.6060975338422168 1104 72-73 -0.5273573332336028 1104 74-75 -0.6156952223383101 1104 76-77 -0.6031463039791376 1104 78-79 -0.380740258983554 1104 80-81 -0.48987742254367816 1104 82-83 -0.5805157666635239 1104 84-85 -0.27909728705436976 1104 86-87 -0.3333438259611725 1104 88-89 -0.43788605597763564 1104 90-91 -0.4069186195938279 1104 92-93 -0.49444314788341615 1104 94-95 -0.31265401116826297 1104 96-97 -0.21074082577126063 1104 98-99 -0.11137401500573674 1104 100-101 0.04634360049209718 1104 102-103 -0.1379994615756921 1104 104-105 -0.2241034404784763 1104 106-107 -0.16672993476062814 1104 108-109 -0.05458539371153037 1104 110-111 0.13031195213305224 1104 112-113 0.25302759327408 1104 114-115 0.24015263402000642 1104 116-117 0.21710907745358554 1104 118-119 0.06316210985923831 1104 120-121 -0.0516247902092104 1104 122-123 -0.030431441320747155 1104 124-125 -0.14392628307562205 1105 1 0.20487466063858406 1105 2 0.5639261665351754 1105 3 0.13084549570471538 1105 4 0.0693501707996731 1105 5 0.44637139129152814 1105 6 0.3075606553310877 1105 7 0.39502729388103575 1105 8 0.36616216120856393 1105 9 0.2908084735345824 1105 10-11 0.4067804834074735 1105 12-13 0.4268267911672794 1105 14-15 0.45445089850773 1105 16-17 0.5273118036674589 1105 18-19 0.3842232638715757 1105 20-21 0.26764933151244463 1105 22-23 0.2979374613340937 1105 24-25 0.3040139300097735 1105 26-27 0.32474781715464474 1105 28-29 0.13052547377546375 1105 30-31 0.14676842152891822 1105 32-33 0.019799463643600745 1105 34-35 0.0021167447594407918 1105 36-37 0.08509100601991548 1105 38-39 -0.03005206960678919 1105 40-41 0.23553741343685175 1105 42-43 -0.2110917674923769 1105 44-45 -0.563317899804936 1105 46-47 -0.5281945941900688 1105 48-49 -0.5731545223589585 1105 50-51 -0.4595733823428745 1105 52-53 -0.47977944368202685 1105 54-55 -0.4756723914833465 1105 56-57 -0.3636165512831653 1105 58-59 -0.5430940220467555 1105 60-61 -0.5576774827381321 1105 62-63 -0.6516733376683916 1105 64-65 -0.5972168721325204 1105 66-67 -0.624261980342304 1105 68-69 -0.5704759845041742 1105 70-71 -0.519072217386519 1105 72-73 -0.6753003712082872 1105 74-75 -0.6987648425914728 1105 76-77 -0.9702349115740745 1105 78-79 -0.9424491197430491 1105 80-81 -0.7857634984930435 1105 82-83 -0.8139018426128928 1105 84-85 -0.8874833630037351 1105 86-87 -0.7882488892523121 1105 88-89 -0.537569600281433 1105 90-91 -0.6766971006064857 1105 92-93 -0.558525426364433 1105 94-95 -0.5381286947125687 1105 96-97 -0.4639053827332873 1105 98-99 -0.529095533993079 1105 100-101 -0.49637791849524504 1105 102-103 -0.4726513603098681 1105 104-105 -0.40448318731392163 1105 106-107 -0.45233120058341214 1105 108-109 -0.5648702038381153 1105 110-111 -0.4282323516644162 1105 112-113 -0.4495040522955378 1105 114-115 -0.43706255585341225 1105 116-117 -0.5392200364704678 1105 118-119 -0.505667004064815 1105 120-121 -0.4701374484370575 1105 122-123 -0.3951466311941694 1105 124-125 -0.4413946375060043 1106 1 0.2568008043642891 1106 2 0.6733949196498941 1106 3 0.34325205406213755 1106 4 0.11398537462704184 1106 5 0.2923048110064741 1106 6 0.2694912842556363 1106 7 0.2723969739368499 1106 8 0.08090849829617497 1106 9 0.007782459381282081 1106 10-11 0.167027916877025 1106 12-13 0.20936441700202835 1106 14-15 0.09948852434247613 1106 16-17 0.04630911254814407 1106 18-19 0.1873629030622439 1106 20-21 0.3583849041228291 1106 22-23 0.039668498910089056 1106 24-25 0.04139432470726945 1106 26-27 0.18620002486917997 1106 28-29 -0.025711444394573846 1106 30-31 -0.04638292901428542 1106 32-33 0.04902177999962731 1106 34-35 0.17453775542424665 1106 36-37 0.21674903452585426 1106 38-39 -0.12849221711999803 1106 40-41 0.04548209598444686 1106 42-43 0.05771616632603127 1106 44-45 0.05315897650324786 1106 46-47 0.16183829726811894 1106 48-49 0.16653574974309748 1106 50-51 -0.16134129822047782 1106 52-53 -0.29358887256670485 1106 54-55 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46-47 -0.8132972838196864 2216 48-49 -1.0919236608583063 2216 50-51 -1.04102415373411 2216 52-53 -1.0227033187771077 2216 54-55 -1.0045191079507454 2216 56-57 -1.629116297728526 2216 58-59 -1.637750357987148 2216 60-61 -1.3823615146470054 2216 62-63 -1.1598187805113263 2216 64-65 -1.3417361910457473 2216 66-67 -1.4535014627007499 2216 68-69 -1.1830262078272966 2216 70-71 -1.1141259514662138 2216 72-73 -1.142972154477583 2216 74-75 -1.211758298931315 2216 76-77 -1.0575308488485575 2216 78-79 -1.0195589869194244 2216 80-81 -1.0255116017092867 2216 82-83 -1.0634495889022162 2216 84-85 -0.8924727819735239 2216 86-87 -0.9732401611213959 2216 88-89 -1.0070894067997358 2216 90-91 -0.9240835373836127 2216 92-93 -0.9717244082450236 2216 94-95 -1.0645520139378633 2216 96-97 -1.3057884647874722 2216 98-99 -1.1687992651471433 2216 100-101 -1.1778194253899805 2216 102-103 -1.1917323222571667 2216 104-105 -1.004272785722378 2216 106-107 -0.9427973022783256 2216 108-109 -0.9136224224412217 2216 110-111 -0.942160790743042 2216 112-113 -0.7126718274510857 2216 114-115 -0.7547506738928504 2216 116-117 -0.7717564605698612 2216 118-119 -0.7726380988357384 2216 120-121 -0.7916678944981825 2216 122-123 -0.7304458256705786 2216 124-125 -0.6850338292518252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 33.0 4 89.0 5 277.0 6 683.0 7 1182.0 8 1933.0 9 3110.0 10 5146.0 11 6466.0 12 7126.0 13 9172.0 14 13336.0 15 9397.0 16 3613.0 17 2971.0 18 3024.0 19 2567.0 20 2617.0 21 2610.0 22 2694.0 23 3142.0 24 3499.0 25 4131.0 26 4895.0 27 5763.0 28 6919.0 29 8158.0 30 9994.0 31 12247.0 32 15000.0 33 18529.0 34 24887.0 35 35929.0 36 53458.0 37 83710.0 38 34529.0 39 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.660727321484444 17.653908992943304 14.039152004528999 17.64621168104326 2 19.596477428095714 23.477609236701646 36.765913732223986 20.159999602978655 3 16.630479825112097 30.886027578095337 35.380557368344 17.102935228448565 4 10.646375567306285 19.503921826496708 35.007109163500836 34.84259344269617 5 9.124544343904887 42.5699500392336 34.43568172111364 13.86982389574787 6 25.451301609673425 38.49394914652817 18.349334120759007 17.705415123039398 7 22.056769089903003 38.034645075322395 23.193490835010337 16.71509499976427 8 27.962957908293028 32.72026620281339 20.395234751278533 18.921541137615044 9 26.680082679695584 11.102701978407001 21.256771075009738 40.96044426688768 10-11 24.0473348701368 27.035788993079425 28.182188133528204 20.734688003255574 12-13 27.137401644164655 27.79844218748837 29.649926426981573 15.414229741365407 14-15 23.06780876474252 26.811844139344565 32.276842985501276 17.843504110411637 16-17 17.442679900744416 28.342431761786603 33.490694789081886 20.724193548387095 18-19 16.678618663477263 22.926245840581043 41.56056684722867 18.83456864871303 20-21 18.803675425123014 21.473147709310894 43.37954496390828 16.34363190165781 22-23 18.56571075506016 22.25515569440274 42.805725047828666 16.37340850270843 24-25 17.331098433998925 22.751680516921795 43.45535122741631 16.461869821662972 26-27 17.274398822831706 22.24423760735085 43.46589710695507 17.015466462862374 28-29 17.345862665353188 22.0385309217595 43.640958707298495 16.97464770558882 30-31 17.372537537127698 22.142624956265617 43.70175260111017 16.783084905496512 32-33 17.20194242694187 22.370167815960755 43.05919836427205 17.368691392825326 34-35 17.542906274309832 22.567465797188838 42.61930196116382 17.27032596733751 36-37 18.089409207421323 22.231706621075382 41.65275024131454 18.02613393018876 38-39 18.121543122721782 22.704286093582894 41.39518264222669 17.77898814146863 40-41 17.88395066017206 22.921407142910315 41.160443770610996 18.034198426306634 42-43 18.2878093264763 22.9189128829941 40.06749505263746 18.725782737892143 44-45 18.33977528647562 23.32839071117922 39.12013053485789 19.211703467487265 46-47 18.92409688935103 23.97144094432498 37.29664267305614 19.80781949326785 48-49 19.46453875188914 24.64866327014277 35.46199259978013 20.424805378187962 50-51 19.722977734983772 24.88125490118224 34.840457212058645 20.555310151775345 52-53 19.54386522173942 25.184689793112693 34.44325054035878 20.828194444789105 54-55 18.93548831374768 25.514705061250925 34.506611476933344 21.04319514806805 56-57 19.51734277968373 25.338015149729205 34.08229988026627 21.0623421903208 58-59 19.771009698836064 24.64846837301001 34.384278381533875 21.196243546620046 60-61 19.940966936273323 24.900215759768436 34.766355372095646 20.39246193186259 62-63 20.394890297222318 24.601734996203454 34.854639477118994 20.148735229455234 64-65 20.0984375794827 24.700586729661424 35.16618630682897 20.034789384026908 66-67 20.159284151859662 24.963987984828425 34.77910769608713 20.097620167224786 68-69 20.270944272676292 24.858560092109638 34.78131405118636 20.089181584027713 70-71 20.160003573280793 25.2793486703177 34.381040767659826 20.179606988741682 72-73 20.372288883071317 25.237379759226886 34.18057611351051 20.20975524419128 74-75 19.745556418534132 25.53517768122343 33.999508681206066 20.71975721903637 76-77 19.207735054770946 25.935957260174714 34.35141496266097 20.504892722393368 78-79 19.66955416984077 25.56147503356071 34.10016352316744 20.668807273431085 80-81 18.863771712158808 25.83213399503722 34.12642679900744 21.177667493796527 82-83 19.28394718623527 25.465073287659333 33.673737782288384 21.577241743817012 84-85 19.099976302758932 25.316345761357617 33.713978022359775 21.86969991352368 86-87 19.19429280397022 25.42704714640199 33.43436724565757 21.94429280397022 88-89 19.340166329400702 25.053381489371468 33.647522118733335 21.958930062494495 90-91 19.175152079861935 25.49234420731745 33.354218595493 21.978285117327612 92-93 18.957166040526253 25.527421972317484 33.24991687303659 22.265495114119673 94-95 18.958346774393664 25.38127118517893 33.2244400661052 22.435941974322198 96-97 19.531880048139556 25.530465638531496 32.907356170672095 22.030298142656857 98-99 19.293816082998134 25.220535140460104 32.79031853835141 22.69533023819036 100-101 19.265876339183247 25.172116452335313 32.97749986042097 22.584507348060473 102-103 19.46307559075829 25.06482646358924 32.843381745364134 22.628716200288338 104-105 19.07610192581123 24.96569470395708 32.98085106910935 22.977352301122338 106-107 19.10071514002551 24.953225574320467 32.769021186209365 23.177038099444662 108-109 19.3470662896576 25.229747652895245 31.849780333079813 23.573405724367333 110-111 19.135106297185466 25.48775116470946 31.77426658974311 23.602875948361962 112-113 19.119008325991736 25.154918042957654 31.833579431946497 23.89249419910412 114-115 18.53816227887843 25.71047422859518 31.79186806232045 23.959495430205934 116-117 18.605462099364146 25.407026670174375 31.706508952104873 24.281002278356606 118-119 18.527052715694595 25.423993805744576 31.64381045975471 24.405143018806115 120-121 18.464802625456755 25.655650749731684 31.31279041633838 24.56675620847318 122-123 18.48056032905063 25.61048693785633 30.98758615555459 24.921366577538446 124-125 18.22563954945543 25.442144627883835 30.761776668263106 25.570439154397622 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 733.0 1 506.0 2 926.0 3 1475.5 4 971.0 5 727.5 6 1166.0 7 3316.5 8 5104.0 9 5447.0 10 4364.0 11 2423.5 12 1851.0 13 1733.5 14 1759.0 15 1812.0 16 1926.0 17 2042.5 18 2145.0 19 2216.0 20 2254.5 21 2412.5 22 2662.5 23 2875.5 24 3172.0 25 3485.0 26 4004.5 27 4422.0 28 4664.5 29 5276.0 30 6038.0 31 6964.0 32 7915.5 33 9089.5 34 10173.0 35 10299.0 36 10979.0 37 12154.0 38 12365.0 39 12311.5 40 11872.5 41 11768.5 42 11963.5 43 11869.5 44 12242.0 45 12595.0 46 12518.5 47 12358.0 48 12009.0 49 11252.5 50 10870.5 51 9989.5 52 9105.0 53 8954.0 54 8690.5 55 8156.5 56 7250.0 57 6257.0 58 5625.0 59 5412.5 60 5007.0 61 4589.5 62 4113.0 63 2986.0 64 1992.5 65 1598.5 66 1282.5 67 1015.5 68 710.0 69 487.5 70 455.0 71 425.0 72 380.0 73 452.0 74 378.0 75 184.0 76 112.5 77 99.5 78 85.5 79 43.5 80 22.5 81 10.5 82 4.5 83 6.5 84 3.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06526038396927056 2 0.0 3 0.0 4 0.0 5 0.07071942749521713 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4813834208848115E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.2406917104424057E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00210917590775209 44-45 0.010794017880848931 46-47 0.011538432907114374 48-49 0.009925533683539246 50-51 0.009181118657273803 52-53 0.006947873578477473 54-55 0.006823804407433232 56-57 0.0058312510390793075 58-59 0.003598005960282977 60-61 0.0016128992235751277 62-63 7.444150262654435E-4 64-65 0.0011166225393981653 66-67 0.0026054525919290525 68-69 7.444150262654435E-4 70-71 0.0024813834208848116 72-73 0.0018610375656636087 74-75 9.925533683539246E-4 76-77 0.0011166225393981653 78-79 0.0 80-81 2.4813834208848115E-4 82-83 0.0 84-85 1.2406917104424057E-4 86-87 2.4813834208848115E-4 88-89 0.0013647608814866465 90-91 0.0013647608814866465 92-93 7.444150262654435E-4 94-95 0.0017369683946193682 96-97 0.001488830052530887 98-99 9.925533683539246E-4 100-101 8.684841973096841E-4 102-103 4.962766841769623E-4 104-105 4.962766841769623E-4 106-107 7.444150262654435E-4 108-109 0.0011166225393981653 110-111 8.684841973096841E-4 112-113 0.011414363736070134 114-115 0.020719551564388178 116-117 0.01960292902499001 118-119 0.011662502078158615 120-121 0.0058312510390793075 122-123 0.0058312510390793075 124-125 0.005459043525946586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 403001.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.04564609835096 #Duplication Level Percentage of deduplicated Percentage of total 1 54.93456115776591 17.054789442722175 2 14.174865330627442 8.801357050928882 3 9.255508437418948 8.620297182252298 4 6.405145379128546 7.954075065956514 5 4.294748659140774 6.66666234765259 6 2.7263033018789637 5.078390848013999 7 1.8514940226621879 4.023657972654773 8 1.2948076662977261 3.215851245864873 9 0.947412434231369 2.6471728028091857 >10 3.858307394975765 20.03802411592842 >50 0.17443138648029166 3.5815734574534934 >100 0.07281310169590158 3.9387868457055477 >500 0.003200575898720948 0.7338027461033166 >1k 0.004000719873401185 1.9336224020542372 >5k 0.0024004319240407113 5.711936473899683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8967 2.2250565135074107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7441 1.8463974034803883 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6586 1.634239120994737 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.6052094163538055 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.3987583157361892 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 0.3660040545805097 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.2940439353748502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1078 0.2674931327713827 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.24019791514164976 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 686 0.17022290267269807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 673 0.16699710422554784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 627 0.15558274048947768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 428 0.10620321041386994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.4813834208848115E-4 0.0 20-21 0.0 0.0 0.0 3.722075131327217E-4 0.0 22-23 0.0 0.0 0.0 6.203458552212029E-4 0.0 24-25 0.0 0.0 0.0 9.925533683539246E-4 0.0 26-27 0.0 0.0 0.0 0.0013647608814866463 0.0 28-29 0.0 0.0 0.0 0.0022332450787963306 0.0 30-31 0.0 0.0 0.0 0.007692288604742917 0.0 32-33 0.0 0.0 0.0 0.020719551564388178 0.0 34-35 0.0 0.0 0.0 0.033126468668812234 0.0 36-37 0.0 0.0 0.0 0.05620333448304099 0.0 38-39 0.0 0.0 0.0 0.09267967077004771 0.0 40-41 0.0 0.0 0.0 0.1265505544651254 0.0 42-43 0.0 0.0 0.0 0.17307649360671562 0.0 44-45 0.0 0.0 0.0 0.22568182212947363 0.0 46-47 0.0 0.0 0.0 0.2833739866650455 0.0 48-49 0.0 0.0 0.0 0.34813809395013906 0.0 50-51 0.0 0.0 0.0 0.41600393051133866 0.0 52-53 0.0 0.0 0.0 0.49317495490085633 0.0 54-55 0.0 0.0 0.0 0.5836213805921078 0.0 56-57 0.0 0.0 0.0 0.6957799112161012 0.0 58-59 0.0 0.0 0.0 0.8167473529842357 0.0 60-61 0.0 0.0 0.0 0.9503698501988829 0.0 62-63 0.0 0.0 0.0 1.092304981873494 0.0 64-65 0.0 0.0 0.0 1.2379621886794325 0.0 66-67 0.0 0.0 0.0 1.4276639512060765 0.0 68-69 0.0 0.0 0.0 1.6297726308371443 0.0 70-71 0.0 0.0 0.0 1.8419309133227957 0.0 72-73 0.0 0.0 0.0 2.058183478452907 0.0 74-75 0.0 0.0 0.0 2.305081128830946 0.0 76-77 0.0 0.0 0.0 2.603715623534433 0.0 78-79 0.0 0.0 0.0 2.926543606591547 0.0 80-81 0.0 0.0 0.0 3.283366542514783 0.0 82-83 0.0 0.0 0.0 3.643911553569346 0.0 84-85 0.0 0.0 0.0 4.023687286135766 0.0 86-87 0.0 0.0 0.0 4.449368611988556 0.0 88-89 0.0 0.0 0.0 4.895769489405733 0.0 90-91 0.0 0.0 0.0 5.37839856476783 0.0 92-93 0.0 0.0 0.0 5.888322857759658 0.0 94-95 0.0 0.0 0.0 6.42727933677584 0.0 96-97 0.0 0.0 0.0 7.002712152079027 0.0 98-99 0.0 0.0 0.0 7.654943784258601 0.0 100-101 0.0 0.0 0.0 8.330748558936577 0.0 102-103 0.0 0.0 0.0 8.984344951997638 0.0 104-105 0.0 0.0 0.0 9.68980225855519 0.0 106-107 0.0 0.0 0.0 10.42007339932159 0.0 108-109 0.0 0.0 0.0 11.165480978955387 0.0 110-111 0.0 0.0 0.0 11.969821414835199 0.0 112-113 0.0 0.0 0.0 12.759769826873878 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 83.87685 1 GACGTGA 15 0.0042374926 59.495037 24-25 GTTATCA 265 0.0 58.459526 1 GTATCAA 5580 0.0 54.417698 2 TATAACG 35 0.007772252 51.033737 2 CACGCAG 95 2.6102498E-9 50.13841 5 CCACGCA 90 9.652649E-8 46.308388 4 CTAATGT 55 8.494104E-4 43.301353 4 CATGGGG 970 0.0 39.89751 4 ATCAACG 7945 0.0 39.867764 4 GTATTAC 90 5.097643E-6 39.7225 1 CCTAATG 60 0.0013032568 39.69291 3 TCAACGC 7995 0.0 39.618435 5 TATCAAC 8065 0.0 39.422215 3 CAACGCA 8120 0.0 39.126045 6 GTACATG 4515 0.0 38.138878 1 AACGCAG 8325 0.0 38.091118 7 TACATGG 4645 0.0 37.043865 2 GGAACGC 65 0.0019382013 36.61233 8 ACATGGG 4680 0.0 36.257942 3 >>END_MODULE