FastQCFastQC Report
Thu 26 May 2016
SRR936268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048290
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33990.16594329904456886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.075.7523651
GTACATG179500.054.422711
TACATGG183650.051.767512
GTATCAA49300.049.8396032
ACATGGG187100.048.809693
CATGGGA121300.045.2701954
ATGGGAG46850.040.6360785
TCAACGC62250.040.5226555
CATGGGG62900.040.4822434
ATCAACG62650.040.2639354
CAACGCA63450.039.8500376
AACGCAG63550.039.6000987
ATGGGAT47750.039.3717885
ATGGGGG36050.036.8019685
TATCAAC71950.034.4807473
ACGCAGA76250.032.4582378
CGCAGAG76650.031.978389
CATGGGC25650.029.2250044
ATGGGCG3900.028.984145
GTCGGGA5500.027.042622
Produced by FastQC (version 0.11.5)