FastQCFastQC Report
Thu 26 May 2016
SRR936268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048290
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122480.5979622026177933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93900.4584311791787296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85510.41747018244486866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.119319041737254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.10887130240346825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.090.508671
GTATCAA103850.059.554682
GTACATG160450.045.5937231
ATCAACG146200.043.972714
TCAACGC146000.043.869865
TACATGG168600.043.2499282
CAACGCA148400.043.2194946
ACATGGG167550.042.7393653
TATCAAC151950.042.4262473
AACGCAG151750.042.3045927
CATGGGA105200.039.5519874
ATGGGAT43950.035.7562455
ACGCAGA179050.035.6549768
CATGGGG59950.035.248944
CGCAGAG180100.035.2488949
GTGGTAT18550.029.8665771
ATGGGGG34350.029.2864555
ATGGGAG37000.029.2803695
AGTACAT106650.028.2978842
GAGTACA110950.027.8667581