##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048290 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49555189938925 31.0 31.0 34.0 30.0 34.0 2 31.37001059420297 31.0 31.0 34.0 30.0 34.0 3 31.72420311576974 31.0 31.0 34.0 30.0 34.0 4 35.55521532595482 37.0 35.0 37.0 33.0 37.0 5 35.18255862207012 37.0 35.0 37.0 33.0 37.0 6 35.38179603474117 37.0 35.0 37.0 33.0 37.0 7 35.1967206792007 37.0 35.0 37.0 32.0 37.0 8 35.28669377871297 37.0 35.0 37.0 32.0 37.0 9 36.95676881691557 39.0 37.0 39.0 33.0 39.0 10-11 36.84196720190988 39.0 37.0 39.0 32.0 39.0 12-13 36.77532478311177 39.0 37.0 39.0 32.0 39.0 14-15 37.87026714967119 40.0 37.0 41.0 32.5 41.0 16-17 37.79048718687295 40.0 37.0 41.0 32.0 41.0 18-19 37.93257985929726 40.0 37.0 41.0 32.0 41.0 20-21 37.99320481963003 40.0 37.0 41.0 32.5 41.0 22-23 37.8925061880886 40.0 37.0 41.0 32.0 41.0 24-25 37.84766585786193 40.0 37.0 41.0 32.0 41.0 26-27 37.657824087409494 40.0 37.0 41.0 32.0 41.0 28-29 37.49127442891387 40.0 36.0 41.0 32.0 41.0 30-31 37.27616304331906 39.0 36.0 41.0 31.5 41.0 32-33 37.0245695189646 39.0 35.5 41.0 31.0 41.0 34-35 36.79890323147601 39.0 35.0 41.0 30.5 41.0 36-37 36.57869417904691 39.0 35.0 41.0 30.0 41.0 38-39 36.55037738796753 39.0 35.0 41.0 30.0 41.0 40-41 36.55247840881906 39.0 35.0 41.0 30.0 41.0 42-43 36.428320208564216 39.0 35.0 41.0 30.0 41.0 44-45 36.21651231026857 39.0 35.0 41.0 30.0 41.0 46-47 35.87027056715601 39.0 35.0 41.0 28.5 41.0 48-49 35.550206025513965 39.0 35.0 41.0 27.0 41.0 50-51 35.36865458504411 39.0 35.0 41.0 26.0 41.0 52-53 35.23615186326155 39.0 35.0 41.0 26.0 41.0 54-55 35.067639347943896 39.0 34.5 41.0 26.0 41.0 56-57 34.87419969828491 38.0 34.0 40.0 25.5 41.0 58-59 34.66653745319266 38.0 34.0 40.0 25.0 41.0 60-61 34.447501330378024 38.0 34.0 40.0 25.0 41.0 62-63 34.22797992471769 37.5 34.0 40.0 24.5 41.0 64-65 33.99023282835927 37.0 33.5 40.0 24.0 41.0 66-67 33.712992300894896 36.5 33.0 40.0 23.0 41.0 68-69 33.40755190915349 36.0 33.0 39.0 23.0 41.0 70-71 33.08407696175834 36.0 33.0 39.0 22.5 41.0 72-73 32.81725805427942 35.0 33.0 39.0 22.5 40.0 74-75 32.63611793251932 35.0 33.0 37.5 23.0 40.0 76-77 32.33479414536028 35.0 33.0 37.0 22.0 39.0 78-79 32.00310600549727 35.0 32.0 37.0 22.0 39.0 80-81 31.72612740383442 35.0 32.0 36.0 21.0 39.0 82-83 31.461718311371925 35.0 32.0 36.0 20.5 37.0 84-85 31.211694388978124 35.0 32.0 35.5 20.0 37.0 86-87 31.00832645767933 35.0 32.0 35.0 20.0 36.5 88-89 30.842548662542903 35.0 32.0 35.0 20.0 36.0 90-91 30.699910657182333 35.0 32.0 35.0 19.0 36.0 92-93 30.56319539713615 35.0 32.0 35.0 18.5 36.0 94-95 30.440189621586786 34.5 31.0 35.0 18.0 35.5 96-97 30.37234571276528 34.0 31.0 35.0 18.0 35.0 98-99 30.2855801668709 34.0 31.0 35.0 17.0 35.0 100-101 30.19917833900473 34.0 31.0 35.0 15.0 35.0 102-103 29.176018776638074 33.5 29.5 34.5 10.0 35.0 104-105 29.918552304605306 34.0 31.0 35.0 10.0 35.0 106-107 30.062121574581724 34.0 31.0 35.0 10.0 35.0 108-109 30.022698934232945 34.0 31.0 35.0 7.0 35.0 110-111 29.97831630286727 34.0 31.0 35.0 7.0 35.0 112-113 29.891698441138708 34.0 31.0 35.0 2.0 35.0 114-115 29.86103261745163 34.0 31.0 35.0 2.0 35.0 116-117 29.75596082585962 34.0 31.0 35.0 2.0 35.0 118-119 29.61948259279692 34.0 31.0 35.0 2.0 35.0 120-121 29.41591522684776 34.0 31.0 35.0 2.0 35.0 122-123 29.178422000790903 34.0 31.0 35.0 2.0 35.0 124-125 27.132650161842317 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.248917995984705 1101 2 0.6418291356013732 1101 3 0.4388424746854973 1101 4 0.2795242863251346 1101 5 0.45692613588239794 1101 6 0.2964127438777382 1101 7 0.45046479978234544 1101 8 0.4498456187455062 1101 9 0.3272082213280427 1101 10-11 0.4286896207116584 1101 12-13 0.5206670331833152 1101 14-15 0.558534540062098 1101 16-17 0.6429526208674119 1101 18-19 0.5623244633930824 1101 20-21 0.47589208282873585 1101 22-23 0.46902971580454533 1101 24-25 0.40610334442844476 1101 26-27 0.41622206152997876 1101 28-29 0.5191849464387914 1101 30-31 0.401686874268151 1101 32-33 0.28163407239705407 1101 34-35 0.27162937400419906 1101 36-37 0.4175869319326253 1101 38-39 0.38509964266012986 1101 40-41 0.32807613312472483 1101 42-43 0.4213098140743128 1101 44-45 0.4087865214422379 1101 46-47 0.31628239863021435 1101 48-49 0.3384308646211096 1101 50-51 0.2550138823887238 1101 52-53 0.3413487309820056 1101 54-55 0.22589192661817492 1101 56-57 0.03379360745343263 1101 58-59 0.2516045152140549 1101 60-61 0.3298147521971728 1101 62-63 0.17788836474624503 1101 64-65 0.1954095454121969 1101 66-67 0.20115042696981078 1101 68-69 0.1419630961051368 1101 70-71 0.0767010266700936 1101 72-73 0.12437984380199651 1101 74-75 0.16831907680724356 1101 76-77 0.19674033323208562 1101 78-79 0.2636211255881662 1101 80-81 0.20998868683302163 1101 82-83 0.15884908861524139 1101 84-85 0.14688807775512913 1101 86-87 0.06987883406629081 1101 88-89 0.10492151861619092 1101 90-91 0.1188751626381972 1101 92-93 0.12792914029163427 1101 94-95 0.09531131991531083 1101 96-97 0.12302816654727522 1101 98-99 0.13978273872695368 1101 100-101 0.1819060382516433 1101 102-103 0.14288860046628926 1101 104-105 0.17682608336511052 1101 106-107 0.12475070709975355 1101 108-109 0.05814637215130247 1101 110-111 0.09659653958092917 1101 112-113 0.13357481092825552 1101 114-115 0.10395194313320388 1101 116-117 0.12772062586895672 1101 118-119 0.13374873128926268 1101 120-121 0.16490815500082334 1101 122-123 0.11891780486507741 1101 124-125 0.18332952309518546 1102 1 0.2976776585963563 1102 2 0.6999137221670928 1102 3 0.47871149837314064 1102 4 0.2810983968582761 1102 5 0.46126576243411677 1102 6 0.3385614185715369 1102 7 0.5209773988838364 1102 8 0.4269694360326284 1102 9 0.4505198404098678 1102 10-11 0.49895145820247677 1102 12-13 0.4752932279852473 1102 14-15 0.5639552738334075 1102 16-17 0.6016462657069965 1102 18-19 0.6327157113753614 1102 20-21 0.5011851561436913 1102 22-23 0.5128174365449851 1102 24-25 0.4207674873602585 1102 26-27 0.5588206794627695 1102 28-29 0.5434524070687559 1102 30-31 0.44206985563718604 1102 32-33 0.4934880273612805 1102 34-35 0.4465746278398868 1102 36-37 0.43718571335553946 1102 38-39 0.4632723296778707 1102 40-41 0.48157401581249815 1102 42-43 0.3654406338045746 1102 44-45 0.4437533743955413 1102 46-47 0.46739309525931816 1102 48-49 0.531481843604567 1102 50-51 0.5168384782112057 1102 52-53 0.47676414556762836 1102 54-55 0.5375510750418755 1102 56-57 0.5186956400271114 1102 58-59 0.5239767568939016 1102 60-61 0.6245851406819369 1102 62-63 0.6001644696286164 1102 64-65 0.6574017761773518 1102 66-67 0.6033877087047443 1102 68-69 0.6310018893177656 1102 70-71 0.6616673039539478 1102 72-73 0.613669628893085 1102 74-75 0.5920647506513532 1102 76-77 0.5684429562123512 1102 78-79 0.5163671839794688 1102 80-81 0.5724619469872536 1102 82-83 0.5253368372180987 1102 84-85 0.480617241399532 1102 86-87 0.4466213279123856 1102 88-89 0.4302265658556266 1102 90-91 0.44694273241508853 1102 92-93 0.5858513880735785 1102 94-95 0.4361801662967686 1102 96-97 0.4000557319030271 1102 98-99 0.3830046915358736 1102 100-101 0.4864961961462555 1102 102-103 0.5390016803669049 1102 104-105 0.47619883393675266 1102 106-107 0.41602586097619465 1102 108-109 0.4695453179854141 1102 110-111 0.5028692641323111 1102 112-113 0.48842551681413227 1102 114-115 0.5476576885445468 1102 116-117 0.44517745641720197 1102 118-119 0.5605083800848476 1102 120-121 0.5678829844299642 1102 122-123 0.6442967680613769 1102 124-125 0.57078015944019 1103 1 0.16077697700617932 1103 2 0.6002463675588388 1103 3 0.3236326299643544 1103 4 0.19988884403561258 1103 5 0.44195534161129757 1103 6 0.2761246361179488 1103 7 0.42513007141063497 1103 8 0.3319995210420075 1103 9 0.3509631380299325 1103 10-11 0.34442791705568965 1103 12-13 0.36953495030326167 1103 14-15 0.4863669414451266 1103 16-17 0.5665463228696623 1103 18-19 0.5069592243310908 1103 20-21 0.3794722963804702 1103 22-23 0.3826440431258149 1103 24-25 0.32370046011730835 1103 26-27 0.2444145398710873 1103 28-29 0.29508843010829366 1103 30-31 0.20145405991681287 1103 32-33 0.2914485369124975 1103 34-35 0.11740996033605455 1103 36-37 0.11960119181164686 1103 38-39 0.14008609646796089 1103 40-41 0.21158303787177601 1103 42-43 0.17758483064616826 1103 44-45 0.14794668062442895 1103 46-47 0.12006401507318998 1103 48-49 0.023413184126468423 1103 50-51 0.11576202945902736 1103 52-53 0.050991818266602706 1103 54-55 0.09721718997786155 1103 56-57 0.05952975580791531 1103 58-59 0.142376936875948 1103 60-61 0.1579203870897672 1103 62-63 -0.10122809555175394 1103 64-65 0.0656499585652881 1103 66-67 0.12351677236190994 1103 68-69 0.08047192942346015 1103 70-71 0.050496708980126925 1103 72-73 0.08972168594555541 1103 74-75 0.11421884191418741 1103 76-77 0.11676721774647092 1103 78-79 0.14895128082083176 1103 80-81 0.22151738559019307 1103 82-83 0.06753528119983088 1103 84-85 0.10659595117618537 1103 86-87 0.07460325624555608 1103 88-89 0.09879605292974603 1103 90-91 0.09797430043980881 1103 92-93 0.0062194329956319905 1103 94-95 0.0667941025087444 1103 96-97 -0.009737219871425395 1103 98-99 0.050913020067390846 1103 100-101 0.09240231622458595 1103 102-103 0.10967975814027042 1103 104-105 0.06497888164117782 1103 106-107 0.06938870157041421 1103 108-109 0.10217877214853033 1103 110-111 0.2119578984037389 1103 112-113 0.17823058982853013 1103 114-115 0.2870243996710613 1103 116-117 0.3376136190346557 1103 118-119 0.3134058844409182 1103 120-121 0.22110418098918672 1103 122-123 0.25023456865320526 1103 124-125 0.285457649517074 1104 1 0.21448143964034116 1104 2 0.43836059356668855 1104 3 0.2248872035459435 1104 4 0.16622756044216658 1104 5 0.4371881096850174 1104 6 0.2927464438410752 1104 7 0.34677155799541026 1104 8 0.24875350573458377 1104 9 0.35828539138881155 1104 10-11 0.266495978964727 1104 12-13 0.20302893588193172 1104 14-15 0.2050407227771629 1104 16-17 0.40275261309041355 1104 18-19 0.3567348834621882 1104 20-21 0.33175857951239607 1104 22-23 0.3558629769228716 1104 24-25 0.25927997121020496 1104 26-27 0.2234964729777289 1104 28-29 0.25581225593006707 1104 30-31 0.1752482681287688 1104 32-33 0.2568626606493325 1104 34-35 0.1461135922490442 1104 36-37 0.18508726523263164 1104 38-39 0.14717510178088844 1104 40-41 -0.017317244204214433 1104 42-43 0.08141560905037437 1104 44-45 0.11242393547861695 1104 46-47 -0.023775956645373242 1104 48-49 0.029369131905490065 1104 50-51 -0.08619671877337964 1104 52-53 -0.14138174597029263 1104 54-55 -0.124897300014716 1104 56-57 -0.0734934416194406 1104 58-59 -0.06449081969689985 1104 60-61 -0.1135756309867304 1104 62-63 -0.08176997497533733 1104 64-65 -0.0642064374339597 1104 66-67 0.00976026043090883 1104 68-69 -0.062472432564220526 1104 70-71 0.06923357897041171 1104 72-73 0.048829482171491634 1104 74-75 -0.0013822863897701154 1104 76-77 -0.005252800787843626 1104 78-79 -0.032866447876710225 1104 80-81 -0.029930376816167836 1104 82-83 0.04330355983478995 1104 84-85 -0.07425814095633498 1104 86-87 -0.03863087051880498 1104 88-89 -0.04483670000638895 1104 90-91 -0.08413898039194834 1104 92-93 -0.12332087359586552 1104 94-95 0.018778695399984002 1104 96-97 0.05414477710844423 1104 98-99 0.032636231155489526 1104 100-101 -0.007167693264097608 1104 102-103 -0.014205967854653778 1104 104-105 -0.046504500243191416 1104 106-107 -0.030989968832653148 1104 108-109 -0.06708902097605218 1104 110-111 -0.025898988644026844 1104 112-113 0.02329264470043313 1104 114-115 -0.00171499017346477 1104 116-117 -0.029113551824384132 1104 118-119 -0.005168210349904001 1104 120-121 0.07283083495504883 1104 122-123 0.05418441709274191 1104 124-125 -0.031815351306264006 1105 1 0.22351811082351958 1105 2 0.57563640690352 1105 3 0.3224082199073841 1105 4 0.15701132138742935 1105 5 0.4084805612781821 1105 6 0.3033974046930936 1105 7 0.3700370707654912 1105 8 0.28602349131087834 1105 9 0.3978664482531826 1105 10-11 0.22538640978191893 1105 12-13 0.2602205745025046 1105 14-15 0.2930832508596808 1105 16-17 0.4536043767164486 1105 18-19 0.3730710068358789 1105 20-21 0.38012334632912825 1105 22-23 0.3736631430140491 1105 24-25 0.2730655387612373 1105 26-27 0.30579337552299535 1105 28-29 0.3069277257204135 1105 30-31 0.26733981496209935 1105 32-33 0.3123564176608582 1105 34-35 0.3320810733452433 1105 36-37 0.3847286758420623 1105 38-39 0.2314295855141566 1105 40-41 0.2573737023697902 1105 42-43 0.20808945114719535 1105 44-45 0.22343621748395037 1105 46-47 0.2500495674888441 1105 48-49 0.23022490211689473 1105 50-51 0.28275588696404697 1105 52-53 0.288057368501498 1105 54-55 0.3503206946528863 1105 56-57 0.2993335321702091 1105 58-59 0.34270427915649293 1105 60-61 0.25708851517350695 1105 62-63 0.19329613368325482 1105 64-65 0.2795957392346011 1105 66-67 0.29166961690044957 1105 68-69 0.3011326565276633 1105 70-71 0.23713882686877952 1105 72-73 0.32787012351654 1105 74-75 0.36250126038529373 1105 76-77 0.31970057712786115 1105 78-79 0.2653863166671968 1105 80-81 0.33653923869733404 1105 82-83 0.3414585272721773 1105 84-85 0.3231772801722137 1105 86-87 0.3662466008158951 1105 88-89 0.3373290779267961 1105 90-91 0.34446621797045296 1105 92-93 0.48311352038988176 1105 94-95 0.4734390826546715 1105 96-97 0.4709950182320348 1105 98-99 0.41993499129943146 1105 100-101 0.4543517860255015 1105 102-103 0.4185391258994393 1105 104-105 0.44607730694535164 1105 106-107 0.5136585079364373 1105 108-109 0.5063431293218947 1105 110-111 0.5150504992415179 1105 112-113 0.5274443777307241 1105 114-115 0.5035718813389849 1105 116-117 0.5159049877388249 1105 118-119 0.5291339881727559 1105 120-121 0.46443284990325395 1105 122-123 0.627462131736447 1105 124-125 0.7153855024606521 1106 1 0.19476787230208004 1106 2 0.5410657909275791 1106 3 0.2750742081130646 1106 4 0.1926050807579145 1106 5 0.38732711702859035 1106 6 0.22362084249124337 1106 7 0.311928250469812 1106 8 0.22243958174003353 1106 9 0.1800981911407007 1106 10-11 0.16066463936220998 1106 12-13 0.24783540553845995 1106 14-15 0.25753537847092645 1106 16-17 0.2465159738560061 1106 18-19 0.21606392842820554 1106 20-21 0.13683193219230816 1106 22-23 0.12831930867968566 1106 24-25 0.16527860882747802 1106 26-27 0.18917933501523976 1106 28-29 0.10328266379570294 1106 30-31 0.08627955308827495 1106 32-33 -0.03237543880829463 1106 34-35 0.12029311663346931 1106 36-37 0.08561029651574614 1106 38-39 0.06102691589133258 1106 40-41 0.10748592102807208 1106 42-43 0.03676540727742861 1106 44-45 0.031861703386198315 1106 46-47 0.11460119261491997 1106 48-49 0.06666052858777505 1106 50-51 0.03215049683384308 1106 52-53 0.15493719004201978 1106 54-55 0.1359121823906051 1106 56-57 0.040865112408823734 1106 58-59 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-0.44576414648354046 2216 76-77 -0.2826712614734177 2216 78-79 -0.25151109590785126 2216 80-81 -0.386550122988254 2216 82-83 -0.4928446774187343 2216 84-85 -0.4200524725910064 2216 86-87 -0.21378433050549006 2216 88-89 -0.27091699079263165 2216 90-91 -0.2575564649985651 2216 92-93 -0.31673716771783234 2216 94-95 -0.39236465553593547 2216 96-97 -0.40724650262109563 2216 98-99 -0.4896412311339837 2216 100-101 -0.7712386591795131 2216 102-103 -0.6758936249571725 2216 104-105 -0.43851140268504807 2216 106-107 -0.4466146652886813 2216 108-109 -0.44327195404593667 2216 110-111 -0.5771976237918679 2216 112-113 -0.5157832371466284 2216 114-115 -0.5779904079091978 2216 116-117 -0.57185328345005 2216 118-119 -0.6425883412676789 2216 120-121 -0.6074009897203219 2216 122-123 -0.6795730189942262 2216 124-125 -0.846546621606695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71.0 3 84.0 4 233.0 5 573.0 6 1437.0 7 2784.0 8 4164.0 9 6775.0 10 10792.0 11 13367.0 12 14700.0 13 18305.0 14 24753.0 15 18967.0 16 9498.0 17 8129.0 18 7978.0 19 7435.0 20 7433.0 21 7763.0 22 8711.0 23 10226.0 24 12744.0 25 16166.0 26 21119.0 27 26579.0 28 33615.0 29 41907.0 30 53361.0 31 66543.0 32 82941.0 33 105620.0 34 143951.0 35 200475.0 36 323824.0 37 520829.0 38 213412.0 39 1026.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.47111062040718 18.74578029904062 11.673390504683564 22.109718575868637 2 18.880682561597904 21.635525151785593 37.41185304381619 22.071939242800315 3 17.34034341867131 28.131653425520938 32.52171204523585 22.006291110571908 4 11.770360530179092 17.391832890768832 36.46990244052475 34.36790413852733 5 10.712137918433728 41.82791071003723 34.978888091496174 12.481063280032869 6 29.90426160358152 37.52325110213886 18.24717203130416 14.325315262975458 7 25.49629202896074 35.27347201812243 22.881281459168378 16.348954493748444 8 27.019025626254095 33.77920118733187 20.400480400724508 18.801292785689526 9 26.98187268404376 11.864970292292595 20.36576851910618 40.78738850455746 10-11 24.333614868988278 26.03024962285614 29.480762001474403 20.155373506681183 12-13 25.684863959693203 25.600354441997958 29.7141518046761 19.000629793632736 14-15 22.7526863871815 26.65569816773992 28.065532712652992 22.526082732425586 16-17 21.018811310318025 28.560544923104114 28.64773965002009 21.772904116557772 18-19 20.342529622270284 26.40121760102329 32.321595086633245 20.934657690073184 20-21 21.880983649776155 25.52390042425633 32.519394226403485 20.075721699564024 22-23 21.398093043465526 25.987946042796672 32.27648428689297 20.33747662684483 24-25 20.99092413671892 26.04130762733793 32.43908333292649 20.528684903016664 26-27 20.772278339492942 26.03986740158864 32.473551108485616 20.7143031504328 28-29 20.62743587452747 26.23377365205789 32.363719182205244 20.775071291209397 30-31 21.05219475757827 25.81106679229992 32.49000385687574 20.646734593246073 32-33 20.752676622939134 26.089591805847807 32.25522264913659 20.902508922076464 34-35 21.20673977652063 26.18545980483697 32.11712223015568 20.490678188486726 36-37 21.33135444688008 26.110414052697617 31.714088337100705 20.8441431633216 38-39 21.16799867206304 26.13096778288231 31.742551103603496 20.958482441451164 40-41 21.203141061427626 26.226774206856113 31.652425915587575 20.917658816128686 42-43 21.217517563236562 26.298803144062845 31.146131938055156 21.337547354645437 44-45 21.214096443956837 26.476191731715048 30.998012295352112 21.311699528976007 46-47 21.450351600683966 26.68173619743934 30.152822521974432 21.71508967990227 48-49 21.73708931649179 26.862839398274506 29.790636245123554 21.60943504011015 50-51 21.65584865789638 26.935059722829823 29.761225860883695 21.6478657583901 52-53 21.67613858292321 26.998452269353955 29.77255194906648 21.552857198656355 54-55 21.30654132416212 27.069064344959447 29.716944283323276 21.90745004755516 56-57 21.662137446769865 27.15605839715416 29.443526014777984 21.73827814129799 58-59 21.86386306774477 26.815249012010156 29.54085355389509 21.78003436634998 60-61 22.078419492509106 26.62009365539959 29.943876575824234 21.35761027626707 62-63 21.635994365963388 26.64948139034744 30.06110049236793 21.65342375132124 64-65 21.32803288653024 26.664331345462355 30.06556800788964 21.94206776011776 66-67 21.691755698431678 27.030536963703945 29.58363257412831 21.69407476373607 68-69 21.662978189897725 26.863998953263508 29.603651079373773 21.869371777464995 70-71 21.418951692534755 26.83537048433427 29.77293482296669 21.97274300016429 72-73 21.667007202017533 27.18221521785352 29.313160946771273 21.83761663335767 74-75 21.354683362365936 27.114807017817945 29.4003189029582 22.130190716857918 76-77 21.230151900952777 27.13677920767983 29.372586841955588 22.26048204941181 78-79 21.506553043155616 27.18606053356833 29.24666245175842 22.060723971517636 80-81 21.54927022934603 27.121895658333905 29.070676599225546 22.25815751309452 82-83 21.617724490909342 27.164219618905477 29.114095234329017 22.103960655856156 84-85 21.41244119771506 26.98200712938419 29.125313951901678 22.48023772099907 86-87 21.27478571670935 27.113704830122227 29.059726409675623 22.5517830434928 88-89 21.441892801032676 26.902023467655077 29.152878581199904 22.503205150112336 90-91 21.39898176690977 26.998043211437643 29.12461052193722 22.47836449971537 92-93 21.547777804629593 26.91814125862279 28.989490442827965 22.54459049391965 94-95 21.254565147501776 26.852917117353307 28.996464566204494 22.896053168940423 96-97 21.421054597303417 26.943887264339665 28.883020494707583 22.75203764364933 98-99 21.246673090006418 26.908455691283255 28.78944293923695 23.05542827947338 100-101 21.119377153338448 26.94123293135241 28.887067300110875 23.052322615198268 102-103 21.222915949091835 26.748439480704878 28.800164430925218 23.22848013927807 104-105 21.122166188745236 26.662059548025553 28.908896466522272 23.306877796706942 106-107 21.17101349085109 26.709424555370198 28.976622790765116 23.142939163013594 108-109 21.312297374785217 26.732870487565 28.596711225397232 23.358120912252556 110-111 21.20354785911577 26.94986397106837 28.520842512794285 23.32574565702157 112-113 21.211012265145893 26.82341510097262 28.512045427912973 23.453527205968516 114-115 20.910464400275995 26.956120685571864 28.436441429057645 23.696973485094492 116-117 20.74207218563597 26.879089096369675 28.569210271690448 23.80962844630391 118-119 20.754220883611595 26.831726894786556 28.4011385405851 24.012913681016748 120-121 20.781996126808096 26.92778935851144 28.390452701579427 23.899761813101037 122-123 20.84792861172568 26.926765940192254 28.220158438261883 24.005147009820185 124-125 20.722780978420076 26.81146860658139 27.969192461673664 24.49655795332487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 238.0 2 861.5 3 1544.5 4 1206.5 5 1110.5 6 1773.0 7 3900.5 8 5791.5 9 6463.5 10 5563.5 11 3667.0 12 3173.0 13 3245.5 14 3540.5 15 3849.5 16 4109.0 17 4447.5 18 4799.0 19 5145.5 20 5741.0 21 6662.0 22 7987.0 23 9714.0 24 11791.0 25 14091.0 26 17658.5 27 21656.5 28 24271.5 29 26833.5 30 30546.5 31 34624.0 32 38122.0 33 41916.0 34 46313.5 35 49231.5 36 53023.0 37 57506.5 38 58495.5 39 58385.5 40 58989.0 41 60813.5 42 62609.0 43 63895.5 44 66679.0 45 70011.5 46 71282.5 47 71698.5 48 73043.0 49 73045.0 50 71561.5 51 68120.0 52 64797.5 53 63382.5 54 60271.5 55 55187.0 56 49748.0 57 43624.5 58 38615.5 59 34776.5 60 30309.5 61 25250.5 62 20707.5 63 15968.0 64 11907.0 65 9653.0 66 7676.5 67 6070.5 68 4570.0 69 3141.5 70 2381.5 71 2056.5 72 1843.5 73 1638.0 74 1252.5 75 1022.0 76 710.0 77 407.0 78 239.5 79 100.5 80 49.0 81 35.0 82 20.0 83 10.5 84 6.0 85 2.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06561570871312167 2 2.929272710407218E-4 3 1.464636355203609E-4 4 4.8821211840120294E-5 5 0.06546924507760132 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.8821211840120294E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.8821211840120294E-5 30-31 0.0 32-33 0.0 34-35 1.2205302960030074E-4 36-37 0.0 38-39 0.0 40-41 7.323181776018044E-5 42-43 0.0023678287742458343 44-45 0.00971542115618394 46-47 0.011131236299547428 48-49 0.008787818131221653 50-51 0.007909036318099489 52-53 0.006346757539215639 54-55 0.006932612081297081 56-57 0.005272690878732992 58-59 0.003197789375527879 60-61 0.001781974232164391 62-63 0.0012205302960030073 64-65 0.0017087424144042105 66-67 0.002489881803846135 68-69 9.276030249622856E-4 70-71 0.0022213651387254737 72-73 0.002172543926885353 74-75 8.787818131221653E-4 76-77 7.079075716817443E-4 78-79 2.441060592006015E-4 80-81 4.638015124811428E-4 82-83 2.685166651206616E-4 84-85 3.1733787696078193E-4 86-87 4.1498030064102256E-4 88-89 0.001171709084162887 90-91 0.0017575636262443307 92-93 5.614439361613833E-4 94-95 0.0012937621137631878 96-97 0.0014402257492835487 98-99 0.001489046961123669 100-101 8.299606012820451E-4 102-103 6.59086359841624E-4 104-105 5.12622724321263E-4 106-107 6.346757539215639E-4 108-109 9.520136308823457E-4 110-111 6.102651480015037E-4 112-113 0.010252454486425262 114-115 0.020651372608370886 116-117 0.019015862011726854 118-119 0.011204468117307607 120-121 0.0052971014846530514 122-123 0.005394743908333293 124-125 0.004393909065610826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2048290.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.633698732843758 #Duplication Level Percentage of deduplicated Percentage of total 1 41.83967449694223 11.56184960128748 2 17.651932131435792 9.755763491454024 3 12.697715503855544 10.526545344867106 4 8.656372502570246 9.56830359421196 5 5.618754248614258 7.763348108004616 6 3.6952286376434946 6.126770095297021 7 2.46271110158022 4.763767165296832 8 1.6158283491659007 3.572105104387102 9 1.0662911370382764 2.6519011238175434 >10 4.3116416268139215 20.234298896179297 >50 0.25091118298676596 4.82244011527607 >100 0.12846077987408067 6.085201158622949 >500 0.0028661129467819663 0.5159964806124786 >1k 0.0010747923550432374 0.5573296599401282 >5k 3.582641183477458E-4 0.8880940961884572 >10k+ 1.791320591738729E-4 0.6062859645569492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12248 0.5979622026177933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9390 0.4584311791787296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8551 0.41747018244486866 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.119319041737254 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2230 0.10887130240346825 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4410605920060147E-5 0.0 16-17 0.0 0.0 0.0 7.323181776018044E-5 0.0 18-19 0.0 0.0 0.0 9.764242368024059E-5 4.8821211840120294E-5 20-21 0.0 0.0 0.0 9.764242368024059E-5 4.8821211840120294E-5 22-23 0.0 0.0 0.0 1.2205302960030073E-4 4.8821211840120294E-5 24-25 0.0 0.0 0.0 1.464636355203609E-4 4.8821211840120294E-5 26-27 0.0 0.0 0.0 1.464636355203609E-4 4.8821211840120294E-5 28-29 0.0 0.0 0.0 5.126227243212631E-4 4.8821211840120294E-5 30-31 0.0 0.0 0.0 0.0019772590795248722 4.8821211840120294E-5 32-33 0.0 0.0 0.0 0.006834969657616842 4.8821211840120294E-5 34-35 0.0 0.0 0.0 0.013157316590912418 9.764242368024059E-5 36-37 0.0 0.0 0.0 0.024435016525980208 9.764242368024059E-5 38-39 0.0 0.0 0.0 0.04555019064683224 9.764242368024059E-5 40-41 0.0 0.0 0.0 0.06883790869456961 9.764242368024059E-5 42-43 0.0 0.0 0.0 0.09778888731576095 9.764242368024059E-5 44-45 0.0 0.0 0.0 0.13367247801824939 9.764242368024059E-5 46-47 0.0 0.0 0.0 0.17907620502956123 9.764242368024059E-5 48-49 0.0 0.0 0.0 0.22643278051447793 9.764242368024059E-5 50-51 0.0 0.0 0.0 0.27237354085603116 9.764242368024059E-5 52-53 0.0 2.929272710407218E-4 0.0 0.3228546738987155 9.764242368024059E-5 54-55 0.0 4.88212118401203E-4 0.0 0.3911555492630438 9.764242368024059E-5 56-57 0.0 4.88212118401203E-4 0.0 0.47537213968725134 9.764242368024059E-5 58-59 0.0 4.88212118401203E-4 0.0 0.5694750255090832 9.764242368024059E-5 60-61 0.0 4.88212118401203E-4 0.0 0.6676544825195652 1.464636355203609E-4 62-63 0.0 4.88212118401203E-4 0.0 0.7693978879943758 1.9528484736048118E-4 64-65 0.0 4.88212118401203E-4 0.0 0.8894736585151517 1.9528484736048118E-4 66-67 0.0 4.88212118401203E-4 0.0 1.028760575895015 1.9528484736048118E-4 68-69 0.0 4.88212118401203E-4 0.0 1.1939227355501418 1.9528484736048118E-4 70-71 0.0 4.88212118401203E-4 0.0 1.3714854830126595 1.9528484736048118E-4 72-73 0.0 4.88212118401203E-4 0.0 1.5533689077230275 1.9528484736048118E-4 74-75 0.0 4.88212118401203E-4 0.0 1.7550981550464047 1.9528484736048118E-4 76-77 0.0 4.88212118401203E-4 0.0 1.9879997461296983 1.9528484736048118E-4 78-79 0.0 4.88212118401203E-4 0.0 2.2518295749137085 1.9528484736048118E-4 80-81 0.0 4.88212118401203E-4 0.0 2.528841130894551 1.9528484736048118E-4 82-83 0.0 4.88212118401203E-4 0.0 2.8251370655522408 3.417484828808421E-4 84-85 0.0 4.88212118401203E-4 0.0 3.1375195895112507 3.417484828808421E-4 86-87 0.0 4.88212118401203E-4 0.0 3.479731873904574 3.417484828808421E-4 88-89 0.0 4.88212118401203E-4 0.0 3.8707165489261772 3.417484828808421E-4 90-91 0.0 4.88212118401203E-4 0.0 4.291213646505133 3.417484828808421E-4 92-93 0.0 4.88212118401203E-4 0.0 4.721377344028434 3.417484828808421E-4 94-95 0.0 4.88212118401203E-4 0.0 5.1685308232720955 6.346757539215638E-4 96-97 0.0 4.88212118401203E-4 0.0 5.644586459925108 7.323181776018044E-4 98-99 0.0 4.88212118401203E-4 0.0 6.1678033872156774 7.323181776018044E-4 100-101 0.0 4.88212118401203E-4 0.0 6.735813776369557 7.323181776018044E-4 102-103 0.0 4.88212118401203E-4 0.0 7.3169082502965885 7.323181776018044E-4 104-105 0.0 4.88212118401203E-4 0.0 7.911697074144774 7.323181776018044E-4 106-107 0.0 4.88212118401203E-4 0.0 8.545469635647297 7.323181776018044E-4 108-109 0.0 4.88212118401203E-4 0.0 9.20426306821788 7.323181776018044E-4 110-111 0.0 4.88212118401203E-4 0.0 9.893765043035899 7.323181776018044E-4 112-113 0.0 4.88212118401203E-4 0.0 10.620444370670167 7.323181776018044E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6865 0.0 90.50867 1 GTATCAA 10385 0.0 59.55468 2 GTACATG 16045 0.0 45.593723 1 ATCAACG 14620 0.0 43.97271 4 TCAACGC 14600 0.0 43.86986 5 TACATGG 16860 0.0 43.249928 2 CAACGCA 14840 0.0 43.219494 6 ACATGGG 16755 0.0 42.739365 3 TATCAAC 15195 0.0 42.426247 3 AACGCAG 15175 0.0 42.304592 7 CATGGGA 10520 0.0 39.551987 4 ATGGGAT 4395 0.0 35.756245 5 ACGCAGA 17905 0.0 35.654976 8 CATGGGG 5995 0.0 35.24894 4 CGCAGAG 18010 0.0 35.248894 9 GTGGTAT 1855 0.0 29.866577 1 ATGGGGG 3435 0.0 29.286455 5 ATGGGAG 3700 0.0 29.280369 5 AGTACAT 10665 0.0 28.297884 2 GAGTACA 11095 0.0 27.866758 1 >>END_MODULE