FastQCFastQC Report
Thu 26 May 2016
SRR936267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23109
Sequences flagged as poor quality0
Sequence length125
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3881.6789995239949802No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.38513133411225065No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT730.3158942403392618No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.31156692197844993No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.22934787312302568No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.21636591804059024No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.21203859967977842No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA450.19472932623653122No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1904020078757194No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG370.16011077935003679No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.15145614262841317No Hit
GTTCTAGACCAATCTTTCTCTTGTTTGGTTAGAGTCACAGCAAGCTGAAC330.14280150590678956No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.14280150590678956No Hit
AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.13847418754597773No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.13847418754597773No Hit
GTCTAGAACACAGCAGACAGCCCCATGGTCCCCAGAGAAGATACCACTCC300.12981955082435415No Hit
AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12549223246354235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.12116491410273054No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11683759574191872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACATA454.118374E-879.333331
GTACATA401.9208856E-674.375011
GGAAACA453.8666294E-666.111111
AACATAA401.7356956E-459.5000042
TCAACAC603.019104E-759.54
ACAACAC1101.8189894E-1254.0909085
GAAACAA708.7543594E-751.02
AACCTCT350.007700461451.05
ATCAACA802.19729E-644.6250043
AACAACA1800.042.972224
AACACAG705.2872732E-542.57
GCAGAGA853.3346514E-642.01
GTACAAA600.0012820939.6666681
AGAACAA757.941996E-539.6666642
GAACAAA1053.0826413E-739.6666641
GTACATG2800.038.251
AAACAAC2100.036.8333323
AACAAAG650.001899886236.6153839
TACATAA650.001899886236.6153832
GAGAACA1303.9062797E-836.6153831