##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23109 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.542169717426113 27.0 10.0 31.0 2.0 34.0 2 22.19061837379376 27.0 12.0 31.0 2.0 34.0 3 22.52265351161885 27.0 12.0 31.0 2.0 34.0 4 24.373534120905276 31.0 10.0 37.0 2.0 37.0 5 26.59989614435934 32.0 19.0 37.0 2.0 37.0 6 24.888052274005798 31.0 8.0 37.0 2.0 37.0 7 25.57626898610931 32.0 11.0 37.0 2.0 37.0 8 24.51252758665455 31.0 8.0 37.0 2.0 37.0 9 24.388117183781212 31.0 8.0 39.0 2.0 39.0 10-11 25.20645635899433 31.0 8.0 39.0 2.0 39.0 12-13 25.350469514042146 31.0 8.0 39.0 2.0 39.0 14-15 25.948742914016183 31.5 8.0 40.0 2.0 41.0 16-17 26.235687394521616 32.0 9.0 40.0 2.0 41.0 18-19 26.419749880998744 32.0 11.5 40.0 2.0 41.0 20-21 26.802479553420746 31.5 14.0 40.0 2.0 41.0 22-23 26.783828811285645 31.0 16.5 40.0 2.0 41.0 24-25 26.91299926435588 31.0 18.0 39.0 2.0 41.0 26-27 26.599636505257692 31.0 13.5 39.0 2.0 41.0 28-29 26.08345233458826 30.0 2.0 39.0 2.0 41.0 30-31 25.609091695876067 30.0 2.0 39.0 2.0 41.0 32-33 25.093859535246008 30.0 2.0 39.0 2.0 41.0 34-35 24.522458782292613 30.0 2.0 39.0 2.0 41.0 36-37 23.99565104504738 30.0 2.0 39.0 2.0 41.0 38-39 23.466549829070924 30.0 2.0 39.0 2.0 41.0 40-41 23.271366134406506 30.0 2.0 39.0 2.0 41.0 42-43 22.925397031459603 29.5 2.0 39.0 2.0 41.0 44-45 22.7873123025661 30.0 2.0 39.0 2.0 41.0 46-47 22.65420398978753 30.0 2.0 38.5 2.0 41.0 48-49 22.341836513912327 29.5 2.0 38.0 2.0 41.0 50-51 22.125557142238954 29.0 2.0 38.0 2.0 41.0 52-53 21.948548184689948 29.5 2.0 38.0 2.0 41.0 54-55 21.755268510104287 29.0 2.0 38.0 2.0 41.0 56-57 21.55218745943139 29.0 2.0 38.0 2.0 41.0 58-59 21.29393309965814 29.0 2.0 38.0 2.0 40.0 60-61 21.113505560604093 28.5 2.0 37.5 2.0 40.0 62-63 20.876195421697176 28.0 2.0 37.0 2.0 40.0 64-65 20.558591890605392 28.0 2.0 36.5 2.0 40.0 66-67 20.305963044701198 28.0 2.0 36.0 2.0 40.0 68-69 20.05644986801679 27.5 2.0 36.0 2.0 40.0 70-71 19.78224934008395 27.0 2.0 35.0 2.0 39.0 72-73 19.443896317452076 26.0 2.0 35.0 2.0 39.0 74-75 19.126292786360295 26.0 2.0 35.0 2.0 39.0 76-77 18.778224934008396 25.0 2.0 35.0 2.0 37.5 78-79 18.467566748885716 24.0 2.0 35.0 2.0 37.0 80-81 18.250183911030334 22.5 2.0 35.0 2.0 37.0 82-83 17.972045523389156 21.0 2.0 34.0 2.0 36.0 84-85 17.9258946730711 20.5 2.0 34.5 2.0 36.0 86-87 17.72190488554243 19.0 2.0 34.5 2.0 36.0 88-89 17.566532519797484 18.0 2.0 34.0 2.0 35.5 90-91 17.3790081786317 15.0 2.0 34.0 2.0 35.0 92-93 17.231533168895236 9.0 2.0 34.0 2.0 35.0 94-95 17.09621792375265 7.0 2.0 34.0 2.0 35.0 96-97 16.993487385866977 3.5 2.0 34.0 2.0 35.0 98-99 16.865030940326278 2.0 2.0 34.0 2.0 35.0 100-101 16.765502618027607 2.0 2.0 34.0 2.0 35.0 102-103 16.12447531264875 2.0 2.0 32.5 2.0 35.0 104-105 16.48065688692717 2.0 2.0 34.0 2.0 35.0 106-107 16.510212471331513 2.0 2.0 34.0 2.0 35.0 108-109 16.38257821627937 2.0 2.0 34.0 2.0 35.0 110-111 16.308754165043922 2.0 2.0 34.0 2.0 35.0 112-113 16.24542386083344 2.0 2.0 34.0 2.0 35.0 114-115 16.121749102081438 2.0 2.0 34.0 2.0 35.0 116-117 15.985676576225714 2.0 2.0 34.0 2.0 35.0 118-119 15.750551733091005 2.0 2.0 34.0 2.0 35.0 120-121 15.55692587303648 2.0 2.0 34.0 2.0 35.0 122-123 15.326669263057683 2.0 2.0 34.0 2.0 35.0 124-125 14.274525076809901 2.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2911481561300775 1101 2 1.2638379569376994 1101 3 1.6775578656793044 1101 4 1.4930883798609642 1101 5 1.2565041377189772 1101 6 -1.1440812609402364 1101 7 0.11592699644688764 1101 8 -0.10363119157432266 1101 9 -1.7656908478845423 1101 10-11 -1.2205787131424835 1101 12-13 0.32835171660701334 1101 14-15 -0.5348292488788182 1101 16-17 -2.395185581110436 1101 18-19 -1.388951434073391 1101 20-21 -0.6118843537750891 1101 22-23 -0.5110350135088915 1101 24-25 -0.5158908701914271 1101 26-27 0.9457466264968524 1101 28-29 1.3165447088822866 1101 30-31 0.9161471324956061 1101 32-33 0.9686624303468356 1101 34-35 1.1812131143379752 1101 36-37 0.945283110470271 1101 38-39 0.6144405961287163 1101 40-41 -0.750563445890073 1101 42-43 -0.7301141082616063 1101 44-45 -1.1987591538306859 1101 46-47 -1.163592716473012 1101 48-49 -1.799954604722167 1101 50-51 -1.5548458292580989 1101 52-53 -1.5167313339831132 1101 54-55 -1.5236283222924207 1101 56-57 -1.2037559720167934 1101 58-59 -0.5241506976066042 1101 60-61 -0.3517614554718449 1101 62-63 -0.8165222256680202 1101 64-65 -0.7049228833419292 1101 66-67 -0.6161783043923634 1101 68-69 -0.42603990340570164 1101 70-71 -1.6113357050230945 1101 72-73 -1.2496885834943967 1101 74-75 -1.01906261179548 1101 76-77 -2.4666638193439105 1101 78-79 -1.8390059119027846 1101 80-81 -1.5195800425256216 1101 82-83 -1.1624209247549366 1101 84-85 -1.3129682263910034 1101 86-87 -1.6331899921171669 1101 88-89 -2.8119388051075447 1101 90-91 -2.789694057617389 1101 92-93 -2.450847060554688 1101 94-95 -2.282023281741571 1101 96-97 -2.54851205785509 1101 98-99 -1.9759725772664218 1101 100-101 -2.2513295012011554 1101 102-103 -1.720170751531743 1101 104-105 -1.793132354408371 1101 106-107 -2.2715960511145497 1101 108-109 -1.9459740169754713 1101 110-111 -1.9680425545248035 1101 112-113 -1.4095856260518769 1101 114-115 -1.2773277650182653 1101 116-117 -1.2371476780938941 1101 118-119 -0.9778152018857771 1101 120-121 -0.7913319490169339 1101 122-123 -0.9893023343270837 1101 124-125 -0.290739817882848 1102 1 3.13499199997392 1102 2 2.4147388578386 1102 3 1.9440743821958222 1102 4 2.2213166080891895 1102 5 3.531278912493754 1102 6 4.163726546867572 1102 7 2.7157768462967375 1102 8 2.367089529146398 1102 9 2.998573416379724 1102 10-11 3.3691360015722296 1102 12-13 3.254402767658064 1102 14-15 2.746702282652709 1102 16-17 4.239198803273947 1102 18-19 3.0922797971578433 1102 20-21 2.5240015321107983 1102 22-23 2.937913935440058 1102 24-25 3.292292312991755 1102 26-27 3.587638518388747 1102 28-29 3.502355519693097 1102 30-31 2.5032342195826907 1102 32-33 1.4198636315480364 1102 34-35 2.2525344356592996 1102 36-37 1.702039867227029 1102 38-39 1.811512668200784 1102 40-41 1.2892263438997134 1102 42-43 1.8021681740206752 1102 44-45 0.9183579632864323 1102 46-47 0.7249207970405038 1102 48-49 1.2094297796622158 1102 50-51 2.0480070235947565 1102 52-53 2.5497101074583277 1102 54-55 1.6554257317616354 1102 56-57 1.3232710550102311 1102 58-59 0.4919904435345366 1102 60-61 1.3411814874711006 1102 62-63 1.9199642608184675 1102 64-65 2.145677717258671 1102 66-67 0.9442571310430701 1102 68-69 0.4189300665642648 1102 70-71 0.2576582889709016 1102 72-73 0.5382243294185187 1102 74-75 1.1521085593756943 1102 76-77 1.1024052497251624 1102 78-79 0.8280361301392567 1102 80-81 1.9440085460629675 1102 82-83 0.9092757719417612 1102 84-85 0.2485933351705576 1102 86-87 0.4970652631380901 1102 88-89 1.2173404741717313 1102 90-91 1.5000957321724009 1102 92-93 1.942546332838706 1102 94-95 0.9090427843244946 1102 96-97 0.4770134676704352 1102 98-99 0.48386225756841483 1102 100-101 0.1112831114114563 1102 102-103 0.4416160352550449 1102 104-105 0.863774552498537 1102 106-107 0.9224730179545233 1102 108-109 1.0123593163578626 1102 110-111 0.87242291094066 1102 112-113 0.3764504099841588 1102 114-115 0.5210956584051587 1102 116-117 1.0646541237079106 1102 118-119 1.2147523656817878 1102 120-121 1.6564908738058897 1102 122-123 1.7667036716789202 1102 124-125 1.285836758693728 1103 1 3.957814822796742 1103 2 4.180504623604364 1103 3 4.260891199012637 1103 4 3.4097550465276285 1103 5 4.6453930266078665 1103 6 3.328140961281985 1103 7 3.5603714408913305 1103 8 2.2852576973145666 1103 9 5.317642485448793 1103 10-11 4.737754620190849 1103 12-13 4.953351716607017 1103 14-15 3.742948528898957 1103 16-17 3.674258863334007 1103 18-19 4.124937454815498 1103 20-21 4.804782312891575 1103 22-23 4.6417427642688835 1103 24-25 5.428553574253023 1103 26-27 6.056857737607967 1103 28-29 5.566544708882287 1103 30-31 5.791147132495606 1103 32-33 6.649217985902386 1103 34-35 5.597879781004643 1103 36-37 3.8341719993591603 1103 38-39 3.947773929462045 1103 40-41 3.9855476652210378 1103 42-43 3.130997002849501 1103 44-45 4.148463068391539 1103 46-47 4.225296172415877 1103 48-49 3.213934284166722 1103 50-51 4.070154170741901 1103 52-53 4.149935332683555 1103 54-55 3.0319272332631364 1103 56-57 2.3656884724276495 1103 58-59 1.9064048579489494 1103 60-61 1.064905211194823 1103 62-63 2.030699996554201 1103 64-65 1.6839660055469565 1103 66-67 2.772710584496526 1103 68-69 2.4767378743720734 1103 70-71 2.041442072754684 1103 72-73 2.0975336387278247 1103 74-75 1.4114929437600772 1103 76-77 1.6722250695449823 1103 78-79 2.105438532541662 1103 80-81 1.9804199574743784 1103 82-83 1.920912408578399 1103 84-85 2.089809551386775 1103 86-87 1.3390322301050546 1103 88-89 1.674172306003566 1103 90-91 1.7241948312715003 1103 92-93 1.5630418283342031 1103 94-95 1.1485322738139843 1103 96-97 1.298710164367133 1103 98-99 0.3851385338446889 1103 100-101 1.0403371654655125 1103 102-103 0.4464959151349248 1103 104-105 -0.45979902107503534 1103 106-107 -0.813262717781214 1103 108-109 -0.5293073503088035 1103 110-111 -0.5930425545248035 1103 112-113 0.1320810406147892 1103 114-115 0.06989445720395793 1103 116-117 -1.1260365669827817 1103 118-119 -0.8111485352191092 1103 120-121 -1.4718875045724893 1103 122-123 -1.5309690009937515 1103 124-125 -1.4574064845495158 1104 1 -1.86500800002608 1104 2 -2.0447206016208597 1104 3 -2.1910607529393147 1104 4 -3.3732779865054034 1104 5 -1.8741264929116532 1104 6 -2.755192372051347 1104 7 -2.5544934239735326 1104 8 -3.957234795177925 1104 9 -1.2446698268635217 1104 10-11 -2.4551883227520968 1104 12-13 -2.19154317828788 1104 14-15 -1.7532977173472908 1104 16-17 -2.5580984940233513 1104 18-19 -3.083395878517834 1104 20-21 -2.6381606300513667 1104 22-23 -2.9945184969923773 1104 24-25 -2.3158157951163503 1104 26-27 -1.7772263464761195 1104 28-29 -1.0516985343609555 1104 30-31 0.23296394931242403 1104 32-33 0.3252690369534399 1104 34-35 1.3876695707944293 1104 36-37 0.6209587861459482 1104 38-39 -0.44524408855597386 1104 40-41 -0.6161790615056901 1104 42-43 -0.6032372313847283 1104 44-45 -0.18975014482167296 1104 46-47 0.6843802564999635 1104 48-49 0.8580784283108649 1104 50-51 0.06152053710826877 1104 52-53 0.33349389124211015 1104 54-55 0.0473176236535231 1104 56-57 -0.14970191796273724 1104 58-59 -0.1971987456546529 1104 60-61 -0.2939536476640363 1104 62-63 -0.1881438472896413 1104 64-65 -0.7867547151737604 1104 66-67 -0.5287158419299018 1104 68-69 -0.36485371721951765 1104 70-71 -0.36396333265071945 1104 72-73 0.4301162213104064 1104 74-75 0.8548112620783961 1104 76-77 0.7645674118873238 1104 78-79 0.625333427436555 1104 80-81 0.1872517893062131 1104 82-83 0.49035685302284193 1104 84-85 0.6404852270624524 1104 86-87 0.06463283070565495 1104 88-89 -0.20157844474718445 1104 90-91 -0.32422859215192545 1104 92-93 -0.42231853202616065 1104 94-95 -0.5639301886484773 1104 96-97 -0.577040586383621 1104 98-99 -0.745867472161315 1104 100-101 -0.8076358075074594 1104 102-103 -0.35568126204225337 1104 104-105 0.05296374168772644 1104 106-107 0.0846351801166847 1104 108-109 0.2420890460875924 1104 110-111 0.45350399202174074 1104 112-113 0.5926666262003764 1104 114-115 0.4129875502970499 1104 116-117 1.321410880464665 1104 118-119 0.9985361494655738 1104 120-121 0.8456800629950791 1104 122-123 1.4288658338410816 1104 124-125 -0.47091999806302987 1105 1 -3.1695564509159944 1105 2 -2.8601457828997 1105 3 -0.5832822427217792 1105 4 -2.4161257122799604 1105 5 -2.015853585858256 1105 6 -2.0234850549781775 1105 7 -3.2749944127672066 1105 8 -2.3942815980783863 1105 9 -2.9513277042531065 1105 10-11 -1.9133293906492597 1105 12-13 -1.8223935408455603 1105 14-15 -2.76450404562679 1105 16-17 -1.642475554010165 1105 18-19 -2.693151976078809 1105 20-21 -2.36459438087536 1105 22-23 -2.6682165853246076 1105 24-25 -3.6496984582673058 1105 26-27 -4.767668007649487 1105 28-29 -4.698699193556738 1105 30-31 -3.9348013769894905 1105 32-33 -4.36128336911116 1105 34-35 -3.972580923602404 1105 36-37 -4.127887621237047 1105 38-39 -4.496331761594863 1105 40-41 -5.595414394399558 1105 42-43 -5.840547712597651 1105 44-45 -5.499572161960764 1105 46-47 -6.044690277448623 1105 48-49 -6.330781162987751 1105 50-51 -5.1395341761415665 1105 52-53 -5.253519951869293 1105 54-55 -5.825457590585103 1105 56-57 -5.386682801285087 1105 58-59 -5.183026036359994 1105 60-61 -4.968970127558567 1105 62-63 -5.042131981765582 1105 64-65 -4.318066514778245 1105 66-67 -4.672750120110521 1105 68-69 -4.942638819394865 1105 70-71 -4.056118902855072 1105 72-73 -4.828618122789788 1105 74-75 -5.551582936998731 1105 76-77 -5.667205824763963 1105 78-79 -5.463328405127715 1105 80-81 -5.500948606211258 1105 82-83 -5.96086265917228 1105 84-85 -5.827873375442493 1105 86-87 -4.838135791575162 1105 88-89 -4.838361569335188 1105 90-91 -5.213813298809802 1105 92-93 -5.736754919633279 1105 94-95 -5.2491642031507855 1105 96-97 -5.1406529792643045 1105 98-99 -4.986473932279971 1105 100-101 -4.7980774686808285 1105 102-103 -4.185279152615754 1105 104-105 -3.3900158232430577 1105 106-107 -3.1398209833638706 1105 108-109 -3.0608114153494554 1105 110-111 -2.822039844497704 1105 112-113 -3.7164961951575677 1105 114-115 -3.72346597640038 1105 116-117 -3.97292003581747 1105 118-119 -3.694278616519922 1105 120-121 -3.063350919206634 1105 122-123 -3.1634215755194948 1105 124-125 -3.530577216256832 1106 1 -4.0294867645048456 1106 2 -3.181400138300397 1106 3 -2.9184738803524404 1106 4 -0.8481814614088776 1106 5 -4.49111490990007 1106 6 -2.183763800622774 1106 7 -0.4896285591086702 1106 8 -1.0298216677647964 1106 9 -0.3736273558210499 1106 10-11 -2.7297056972694698 1106 12-13 -2.42204510878981 1106 14-15 -2.4038768679264386 1106 16-17 -3.2229633588882116 1106 18-19 -2.5691101642321215 1106 20-21 -3.3841065759973077 1106 22-23 -3.2427810452549224 1106 24-25 -7.341684520985076 1106 26-27 -5.904253373503149 1106 28-29 -6.36559814826057 1106 30-31 -6.104487788139313 1106 32-33 -7.9226074109230105 1106 34-35 -5.579897996773134 1106 36-37 -6.0404311752440165 1106 38-39 -5.636749880061764 1106 40-41 -5.30691265223928 1106 42-43 -4.66424109238859 1106 44-45 -4.62256867764021 1106 46-47 -4.602878430758725 1106 48-49 -4.538446668214231 1106 50-51 -5.249290273702542 1106 52-53 -4.88696942922121 1106 54-55 -4.412914036578133 1106 56-57 -5.032327400588223 1106 58-59 -5.141214189670098 1106 60-61 -5.655729709440099 1106 62-63 -4.966522225668019 1106 64-65 -5.2755578039768505 1106 66-67 -5.1086386218526805 1106 68-69 -4.519293871659674 1106 70-71 -4.187129355816744 1106 72-73 -3.7969108057166174 1106 74-75 -3.9571578498907165 1106 76-77 -3.825393978074068 1106 78-79 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0.7580093979969895 2215 96-97 1.0429912101187675 2215 98-99 1.0526221939754095 2215 100-101 1.0329842242890415 2215 102-103 0.8541756536970126 2215 104-105 0.7477173187942441 2215 106-107 0.967783033852772 2215 108-109 0.5858887281225691 2215 110-111 1.511532608873889 2215 112-113 1.938453589634399 2215 114-115 1.2202212545895748 2215 116-117 1.0471660473963027 2215 118-119 1.8236553863495164 2215 120-121 2.1310536718980977 2215 122-123 1.5564492996598407 2215 124-125 1.9396523389798972 2216 1 -0.08662962164770249 2216 2 -0.5242572811575386 2216 3 -0.6041881660667272 2216 4 -0.07675288998030894 2216 5 -1.9196863384714966 2216 6 -1.0409066577656318 2216 7 -0.9467714162515257 2216 8 0.25589261794948825 2216 9 0.8835155013218063 2216 10-11 -1.0725628401266114 2216 12-13 0.6493834626387596 2216 14-15 -1.7038768679264393 2216 16-17 -2.437249073173927 2216 18-19 -0.9976815928035485 2216 20-21 -1.2841065759973134 2216 22-23 -0.5142096166834946 2216 24-25 -0.41311309241364924 2216 26-27 -0.3185390877888601 2216 28-29 -0.8370267196891419 2216 30-31 -0.4616306452821739 2216 32-33 -0.9083216966372909 2216 34-35 0.17724486036971854 2216 36-37 1.2024259676131308 2216 38-39 0.7918215485096667 2216 40-41 0.5502302049035777 2216 42-43 0.8929017647542672 2216 44-45 1.906002750931222 2216 46-47 1.4542644263841282 2216 48-49 1.6901247603572003 2216 50-51 1.6364240120117444 2216 52-53 0.5273162850645079 2216 54-55 0.40137167770758 2216 56-57 0.1533868851260607 2216 58-59 0.030214381758472797 2216 60-61 -1.0414439951543848 2216 62-63 -1.6093793685251647 2216 64-65 0.41015648173743813 2216 66-67 0.31993280671875013 2216 68-69 0.894991842626041 2216 70-71 -0.2299864986738882 2216 72-73 0.5316606228548082 2216 74-75 -0.10001499274785886 2216 76-77 0.07460602192593413 2216 78-79 0.4558353579384864 2216 80-81 0.0760548781093 2216 82-83 0.5760711387371273 2216 84-85 0.26480955138677587 2216 86-87 0.6719687380415635 2216 88-89 0.4428230996543583 2216 90-91 0.7182424503191172 2216 92-93 1.133279923572296 2216 94-95 1.315992591274302 2216 96-97 1.6816466723036392 2216 98-99 1.3522020259081806 2216 100-101 0.09390859403694307 2216 102-103 0.18022607386508227 2216 104-105 -0.11614822742424025 2216 106-107 -0.40532620984470924 2216 108-109 -0.20192639792785272 2216 110-111 0.1148939534117055 2216 112-113 -0.11196657843282765 2216 114-115 0.49291033021982855 2216 116-117 0.7723761314299153 2216 118-119 -0.038529487600063916 2216 120-121 0.5352553525703669 2216 122-123 0.3829198878951381 2216 124-125 0.5425935154504842 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 3329.0 3 472.0 4 376.0 5 677.0 6 1375.0 7 1149.0 8 567.0 9 409.0 10 457.0 11 353.0 12 326.0 13 335.0 14 309.0 15 278.0 16 245.0 17 199.0 18 163.0 19 115.0 20 125.0 21 127.0 22 137.0 23 201.0 24 329.0 25 399.0 26 459.0 27 326.0 28 307.0 29 357.0 30 396.0 31 450.0 32 527.0 33 695.0 34 843.0 35 1199.0 36 1683.0 37 2440.0 38 974.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.482106538578044 23.224717642476957 10.117270327578 15.175905491367 2 16.746722056341685 33.11264009693193 30.888398459474665 19.25223938725172 3 17.633822320308106 44.79207235276299 21.64957375914146 15.924531567787442 4 9.269115928858886 29.118525249902632 26.25384049504522 35.358518326193256 5 8.702237223592538 58.14617681422823 22.424163745726773 10.727422216452464 6 21.035094551906184 45.6402267514821 15.79471201696309 17.529966679648624 7 20.23886797351681 52.611536630749924 13.514215240815266 13.635380154917998 8 20.8576744991129 49.1583365788221 14.219568133627591 15.764420788437405 9 20.90527500108183 33.84828421826994 12.813189666363755 32.43325111428448 10-11 17.55160327145268 44.94569215457181 17.597040114241207 19.9056644597343 12-13 19.730408066121424 46.06646761002207 17.82638798736423 16.376736336492275 14-15 20.641755198303656 44.256442434601986 17.417833264815975 17.683969102278382 16-17 18.42651895447464 43.623420460446596 20.35875021637528 17.591310368703482 18-19 18.439501471351914 40.501125151462695 22.959581097455427 18.099792279729964 20-21 22.087588713865326 38.33088108014541 22.896832265881944 16.68469794010732 22-23 21.14039456872469 39.41139528336618 21.41542326266323 18.0327868852459 24-25 19.220200359175195 40.073998745050524 21.965943267628795 18.739857628145487 26-27 19.30025748101348 38.46636519029794 23.01317696951338 19.220200359175195 28-29 19.012073218226668 40.62702843048163 22.748712622787657 17.612185728504045 30-31 20.137175992037733 41.25665325197975 21.755593058981347 16.850577697001167 32-33 18.06871781556969 42.109134969059674 22.718421394261977 17.103725821108657 34-35 17.13401704963434 43.82491669912155 22.153706348176037 16.88735990306807 36-37 17.114544117010688 43.82491669912155 21.87675797308408 17.18378121078368 38-39 17.359037604396555 44.07157384568783 21.18005971699338 17.38932883292224 40-41 16.896014539789693 45.6748452983686 21.46349906962655 15.965641092215154 42-43 16.53901077502272 45.97992124280583 21.72313817127526 15.757929810896186 44-45 17.237872690293827 45.2724046908131 20.327577999913455 17.162144618979617 46-47 16.872214288805225 45.81764680427539 20.97018477649401 16.339954130425376 48-49 15.602146349906961 45.354623739668526 20.771128131896663 18.272101778527848 50-51 15.74096111820326 44.61129021788519 21.009585217560637 18.638163446350912 52-53 16.167211199342233 44.64807321981089 20.111647229374473 19.073068351472404 54-55 16.307499242719288 44.35717685750141 19.67415292743087 19.661170972348437 56-57 16.545501752563936 42.931325457613916 20.35137825089792 20.171794538924228 58-59 16.748885715522093 41.39729109870613 20.6001990566446 21.25362412912718 60-61 16.119260894023974 39.84162014799429 21.201696308797437 22.8374226491843 62-63 16.114933575663162 39.09515773075425 20.952875503050763 23.837033190531827 64-65 16.313990220260504 37.80561685923233 21.260115106668398 24.620277813838765 66-67 17.45640226751482 36.53554892033407 21.94383140767666 24.064217404474448 68-69 17.097234843567442 36.55502185295772 22.863386559349173 23.484356744125666 70-71 17.274654896360726 36.39491107360768 23.326409623956035 23.004024406075555 72-73 17.0085248171708 35.94919728244407 24.566186334328616 22.476091566056514 74-75 16.82245012765589 34.96689601453979 25.37755852698083 22.83309533082349 76-77 17.733350642606776 32.99796616037042 27.05006707343459 22.218616123588212 78-79 18.67670604526375 33.03474836643731 26.643299147518285 21.64524644078065 80-81 17.869661170972346 34.097105024016614 26.844519451296033 21.188714353715003 82-83 18.202864684754857 34.24207018910381 26.552425461941237 21.002639664200096 84-85 18.717815569691464 34.26803409926868 25.54632394305249 21.467826387987365 86-87 18.360811804924488 34.09494136483621 25.53766930633087 22.006577523908433 88-89 18.661560431000908 35.057769700116836 24.65056904236445 21.630100826517808 90-91 19.102946903803712 33.98459474663551 24.882080574667878 22.030377774892898 92-93 18.661560431000908 35.111861179626985 24.139945475788654 22.08663291358345 94-95 18.083863429832533 37.377212341511964 23.711540958068287 20.827383270587216 96-97 17.491020814401313 38.939374269765025 22.44147301917002 21.12813189666364 98-99 16.71859448699641 39.88056601324159 21.86161235882124 21.53922714094076 100-101 16.510883205677445 41.86031416331299 20.84685620321087 20.781946427798694 102-103 15.45069020727855 45.27673200917391 20.005192782032974 19.267385001514562 104-105 15.082868146609545 47.135315245142586 19.477259942013934 18.304556666233935 106-107 14.24120472543165 48.55034834912805 19.31498550348349 17.893461421956815 108-109 14.953048595785193 49.188627807347785 19.30633086676187 16.55199273010515 110-111 14.593881171837811 49.236228309316715 18.886580985763125 17.28330953308235 112-113 14.355878661993163 49.893980700160114 18.462503786403566 17.28763685144316 114-115 14.057293695097147 49.58890475572288 18.620450906573197 17.733350642606776 116-117 14.905448093816261 49.02635336881735 18.503613310831277 17.564585226535115 118-119 14.872993206110174 47.76926738500151 19.41667748496257 17.941061923925744 120-121 15.121814011856852 46.83889393742698 19.55298801332814 18.48630403738803 122-123 15.175905491367 46.10324981608897 19.82128175169847 18.899562940845556 124-125 15.866112769916482 45.867410965424725 19.429659440045004 18.836816824613788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 202.0 1 206.5 2 213.0 3 189.0 4 127.5 5 96.5 6 90.5 7 89.5 8 97.5 9 94.0 10 92.5 11 82.5 12 68.0 13 60.0 14 64.0 15 72.0 16 72.0 17 92.0 18 114.5 19 120.0 20 129.5 21 147.0 22 175.5 23 238.0 24 280.0 25 357.5 26 438.5 27 462.5 28 487.0 29 522.0 30 566.5 31 587.5 32 587.5 33 625.5 34 684.5 35 714.0 36 764.5 37 836.0 38 854.5 39 707.0 40 619.5 41 586.5 42 512.5 43 549.0 44 583.0 45 608.0 46 577.0 47 514.5 48 487.0 49 499.0 50 614.0 51 633.0 52 515.5 53 454.0 54 389.5 55 316.5 56 281.5 57 233.0 58 191.0 59 183.5 60 174.5 61 142.0 62 130.0 63 128.0 64 105.5 65 88.5 66 76.0 67 72.0 68 78.5 69 55.5 70 36.5 71 43.5 72 45.0 73 38.5 74 41.0 75 47.5 76 40.5 77 28.0 78 21.5 79 14.5 80 13.5 81 9.5 82 5.5 83 5.5 84 2.5 85 1.5 86 2.0 87 1.5 88 0.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002163659180405902 16-17 0.004327318360811804 18-19 0.004327318360811804 20-21 0.004327318360811804 22-23 0.08871002639664201 24-25 0.002163659180405902 26-27 0.002163659180405902 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.002163659180405902 52-53 0.002163659180405902 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 23109.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.53762603314726 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17017567386348 53.67605694750963 2 6.638058200348667 8.568090354407373 3 3.701220329891377 7.166039205504349 4 2.2260962853694517 5.746678783158076 5 1.508649590988333 4.8682331559132805 6 0.838138661660185 3.2454887706088535 7 0.46265254123642213 2.0900947682721016 8 0.34866568325063696 1.8001644380977109 9 0.2547941531446963 1.4799428793976372 >10 0.8180233337803405 8.43827080358302 >50 0.026820437173125926 1.241940369552988 >100 0.0067051092932814815 1.6789995239949802 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 388 1.6789995239949802 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.38513133411225065 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 73 0.3158942403392618 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.31156692197844993 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.22934787312302568 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.21636591804059024 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.21203859967977842 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 45 0.19472932623653122 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.1904020078757194 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37 0.16011077935003679 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.15145614262841317 No Hit GTTCTAGACCAATCTTTCTCTTGTTTGGTTAGAGTCACAGCAAGCTGAAC 33 0.14280150590678956 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.14280150590678956 No Hit AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.13847418754597773 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.13847418754597773 No Hit GTCTAGAACACAGCAGACAGCCCCATGGTCCCCAGAGAAGATACCACTCC 30 0.12981955082435415 No Hit AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.12549223246354235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.12116491410273054 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.11683759574191872 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.004327318360811805 0.0 16-17 0.0 0.0 0.0 0.012981955082435416 0.0 18-19 0.0 0.0 0.0 0.021636591804059024 0.0 20-21 0.0 0.0 0.0 0.021636591804059024 0.0 22-23 0.0 0.0 0.0 0.030291228525682633 0.0 24-25 0.0 0.0 0.0 0.04327318360811805 0.0 26-27 0.0 0.0 0.0 0.04327318360811805 0.0 28-29 0.0 0.0 0.0 0.05625513869055346 0.0 30-31 0.0 0.0 0.0 0.08221904885542429 0.0 32-33 0.0 0.0 0.0 0.14712882426760138 0.0 34-35 0.0 0.0 0.0 0.21203859967977845 0.0 36-37 0.0 0.0 0.0 0.3007486260764204 0.0 38-39 0.0 0.0 0.0 0.4413864728028041 0.0 40-41 0.0 0.0 0.0 0.5690423644467524 0.0 42-43 0.0 0.0 0.0 0.6815526418278592 0.0 44-45 0.0 0.0 0.0 0.7854082824873425 0.0 46-47 0.0 0.0 0.0 0.9130641741312908 0.0 48-49 0.0 0.0 0.0 1.0082651780691505 0.0 50-51 0.0 0.0 0.0 1.1467393656151283 0.0 52-53 0.0 0.0 0.0 1.2332857328313644 0.0 54-55 0.0 0.0 0.0 1.3912328530009952 0.0 56-57 0.0 0.0 0.0 1.5708165649746852 0.0 58-59 0.0 0.0 0.0 1.7114544117010688 0.0 60-61 0.0 0.0 0.0 1.8585832359686703 0.0 62-63 0.0 0.0 0.0 2.0446579254835777 0.0 64-65 0.0 0.0 0.0 2.219914319096456 0.0 66-67 0.0 0.0 0.0 2.4016616902505517 0.0 68-69 0.0 0.0 0.0 2.6245185858323596 0.0 70-71 0.0 0.0 0.0 2.9101215976459387 0.0 72-73 0.0 0.0 0.0 3.169760699294647 0.0 74-75 0.0 0.0 0.0 3.4705093253710677 0.0 76-77 0.0 0.0 0.0 3.6955298801332814 0.0 78-79 0.0 0.0 0.0 3.9183867757150894 0.0 80-81 0.0 0.0 0.0 4.212644424250292 0.0 82-83 0.0 0.0 0.0 4.565320870656454 0.0 84-85 0.0 0.0 0.0 4.961270500670734 0.0 86-87 0.0 0.0 0.0 5.409147951014756 0.0 88-89 0.0 0.0 0.0 5.805097581029036 0.0 90-91 0.0 0.0 0.0 6.216192825306157 0.0 92-93 0.0 0.0 0.0 6.562378294171102 0.0 94-95 0.0 0.0 0.0 6.982128175169847 0.0 96-97 0.0021636591804059025 0.0 0.0 7.386732441905751 0.0 98-99 0.004327318360811805 0.0 0.0 7.8021550045436845 0.0 100-101 0.004327318360811805 0.0 0.0 8.297632956856637 0.0 102-103 0.004327318360811805 0.0 0.0 8.816911160154053 0.0 104-105 0.004327318360811805 0.0 0.0 9.236661041152797 0.0 106-107 0.004327318360811805 0.0 0.0 9.870613181011727 0.0 108-109 0.004327318360811805 0.0 0.0 10.420182612834825 0.0 110-111 0.004327318360811805 0.0 0.0 10.928642520230213 0.0 112-113 0.004327318360811805 0.0 0.0 11.467393656151284 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACATA 45 4.118374E-8 79.33333 1 GTACATA 40 1.9208856E-6 74.37501 1 GGAAACA 45 3.8666294E-6 66.11111 1 AACATAA 40 1.7356956E-4 59.500004 2 TCAACAC 60 3.019104E-7 59.5 4 ACAACAC 110 1.8189894E-12 54.090908 5 GAAACAA 70 8.7543594E-7 51.0 2 AACCTCT 35 0.0077004614 51.0 5 ATCAACA 80 2.19729E-6 44.625004 3 AACAACA 180 0.0 42.97222 4 AACACAG 70 5.2872732E-5 42.5 7 GCAGAGA 85 3.3346514E-6 42.0 1 GTACAAA 60 0.00128209 39.666668 1 AGAACAA 75 7.941996E-5 39.666664 2 GAACAAA 105 3.0826413E-7 39.666664 1 GTACATG 280 0.0 38.25 1 AAACAAC 210 0.0 36.833332 3 AACAAAG 65 0.0018998862 36.615383 9 TACATAA 65 0.0018998862 36.615383 2 GAGAACA 130 3.9062797E-8 36.615383 1 >>END_MODULE