##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936267_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23109 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29936388420096 31.0 31.0 34.0 30.0 34.0 2 30.981998355619023 31.0 31.0 34.0 28.0 34.0 3 31.21870266995543 31.0 31.0 34.0 28.0 34.0 4 35.24691678566792 35.0 35.0 37.0 33.0 37.0 5 34.933402570427106 35.0 35.0 37.0 32.0 37.0 6 35.21952486044398 37.0 35.0 37.0 32.0 37.0 7 34.95516898178199 37.0 35.0 37.0 32.0 37.0 8 35.120645635899436 37.0 35.0 37.0 32.0 37.0 9 36.55078108096413 39.0 35.0 39.0 32.0 39.0 10-11 36.57031892336319 39.0 35.0 39.0 32.0 39.0 12-13 36.479250508459906 39.0 35.0 39.0 32.0 39.0 14-15 37.10383400406768 39.0 36.0 41.0 31.5 41.0 16-17 37.12371803193561 39.0 36.0 40.5 31.5 41.0 18-19 37.70857674499113 39.5 36.5 41.0 32.0 41.0 20-21 37.844043446276345 40.0 37.0 41.0 32.0 41.0 22-23 37.64409970141503 40.0 36.5 41.0 32.0 41.0 24-25 37.449110736076854 40.0 36.0 41.0 32.0 41.0 26-27 36.9749664632827 39.0 35.0 41.0 31.5 41.0 28-29 36.63163702453589 39.0 35.0 41.0 31.0 41.0 30-31 35.72324635423428 37.5 35.0 40.0 30.0 41.0 32-33 34.64228222770349 35.5 33.5 40.0 28.5 41.0 34-35 33.89718291574711 35.0 33.0 40.0 25.0 41.0 36-37 33.107122766021895 35.0 33.0 40.0 20.0 41.0 38-39 32.62912717988662 35.0 33.0 40.0 13.0 41.0 40-41 31.877601800164438 35.0 33.0 40.0 2.0 41.0 42-43 31.034467090743867 35.0 31.5 40.0 2.0 41.0 44-45 29.839434852222077 35.0 28.5 40.0 2.0 41.0 46-47 28.038556406594832 35.0 17.5 40.0 2.0 41.0 48-49 26.486022761694578 35.0 2.0 40.0 2.0 41.0 50-51 25.61768142282228 35.0 2.0 40.0 2.0 41.0 52-53 25.27707819464278 35.0 2.0 40.0 2.0 41.0 54-55 24.935458046648492 34.0 2.0 39.0 2.0 41.0 56-57 24.67237872690294 34.0 2.0 39.0 2.0 41.0 58-59 24.38710026396642 34.0 2.0 39.0 2.0 41.0 60-61 24.149487212774243 33.5 2.0 39.0 2.0 41.0 62-63 23.883270587217105 33.0 2.0 38.0 2.0 41.0 64-65 23.664394824527243 33.0 2.0 38.0 2.0 40.5 66-67 23.457635553247652 33.0 2.0 37.5 2.0 40.0 68-69 23.164265004976414 33.0 2.0 37.0 2.0 40.0 70-71 22.887230083517245 33.0 2.0 36.5 2.0 40.0 72-73 22.596823748323164 32.0 2.0 36.0 2.0 39.0 74-75 22.341555238218874 32.0 2.0 36.0 2.0 39.0 76-77 22.07021074040417 32.0 2.0 35.0 2.0 39.0 78-79 21.752931758189447 31.5 2.0 35.0 2.0 37.5 80-81 21.449240555627675 31.0 2.0 35.0 2.0 37.0 82-83 21.2446665801203 31.0 2.0 35.0 2.0 37.0 84-85 20.982712363148558 31.0 2.0 35.0 2.0 36.0 86-87 20.77153922714094 31.0 2.0 35.0 2.0 36.0 88-89 20.60816132242849 30.5 2.0 35.0 2.0 36.0 90-91 20.459669392877235 30.0 2.0 35.0 2.0 35.0 92-93 20.286187199792288 30.0 2.0 35.0 2.0 35.0 94-95 20.189861093080616 30.0 2.0 35.0 2.0 35.0 96-97 20.045415206196722 30.0 2.0 35.0 2.0 35.0 98-99 19.90655155999827 29.0 2.0 35.0 2.0 35.0 100-101 19.760309835994633 29.0 2.0 35.0 2.0 35.0 102-103 19.000346185468864 26.5 2.0 34.0 2.0 35.0 104-105 19.447617811242374 29.0 2.0 34.5 2.0 35.0 106-107 19.465922367908608 29.0 2.0 35.0 2.0 35.0 108-109 19.40228915141287 29.0 2.0 35.0 2.0 35.0 110-111 19.337920290795793 29.0 2.0 35.0 2.0 35.0 112-113 19.194534596910295 29.0 2.0 35.0 2.0 35.0 114-115 19.089683673027825 29.0 2.0 35.0 2.0 35.0 116-117 18.9440261370029 28.0 2.0 35.0 2.0 35.0 118-119 18.786446838893937 27.0 2.0 35.0 2.0 35.0 120-121 18.594898091652603 27.0 2.0 35.0 2.0 35.0 122-123 18.381799298974425 25.0 2.0 35.0 2.0 35.0 124-125 17.005517330910035 13.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9460542303015487 1101 2 1.1954832153351127 1101 3 0.9363825510300927 1101 4 0.8527075925281977 1101 5 1.109977679780215 1101 6 0.3127327391948782 1101 7 0.4872522886737727 1101 8 0.6751401711676337 1101 9 0.42685883587550677 1101 10-11 0.05441385711353064 1101 12-13 0.6853604321104996 1101 14-15 0.1326572599624356 1101 16-17 0.6447817764971333 1101 18-19 0.6545910295330586 1101 20-21 0.5601811279073416 1101 22-23 -0.7963593043098953 1101 24-25 0.08751673980875552 1101 26-27 0.1542206378502371 1101 28-29 -0.34610981577840505 1101 30-31 -0.7900566947860739 1101 32-33 -1.0668599865350998 1101 34-35 -1.5788917914226204 1101 36-37 -1.94991310860906 1101 38-39 -1.6846507415607803 1101 40-41 -0.6150132127130021 1101 42-43 -2.0699731183652403 1101 44-45 -2.117781769196359 1101 46-47 -3.034262322211955 1101 48-49 -3.341464312090917 1101 50-51 -1.9894779999825403 1101 52-53 -3.256751535108098 1101 54-55 -2.152052851858727 1101 56-57 -3.535446656253505 1101 58-59 -2.396429118640963 1101 60-61 -2.142985195564986 1101 62-63 -1.78234561580064 1101 64-65 -1.5672737003496024 1101 66-67 -1.4749750373612436 1101 68-69 -1.3038311079885325 1101 70-71 -0.7337864472022559 1101 72-73 -1.2511961894732018 1101 74-75 -1.9104598477509 1101 76-77 -1.1780587190121459 1101 78-79 -2.3184223865040288 1101 80-81 -2.591554784754102 1101 82-83 -1.490187911220513 1101 84-85 -1.858145794752204 1101 86-87 -2.760447678595316 1101 88-89 -3.1163035065188893 1101 90-91 -2.554035003322465 1101 92-93 -2.5227252831956086 1101 94-95 -2.7422473005682377 1101 96-97 -2.07409380328518 1101 98-99 -1.501533211850969 1101 100-101 -1.335024105552172 1101 102-103 -1.8911435660001779 1101 104-105 -1.4344384147079232 1101 106-107 -2.134302261508605 1101 108-109 -2.3913906420192603 1101 110-111 -2.1939590158819513 1101 112-113 -1.8490292531595323 1101 114-115 -1.3726423944436377 1101 116-117 -1.397570827771517 1101 118-119 -1.4361025351018881 1101 120-121 -1.0148958488223307 1101 122-123 -1.6119945916802791 1101 124-125 -0.7040008174273495 1102 1 -0.9270688928215769 1102 2 0.4875252573771576 1102 3 0.3643104789580214 1102 4 0.39024513006572903 1102 5 -0.3217040019014661 1102 6 -0.32465464819251366 1102 7 -0.01124620982472635 1102 8 -0.07561057958312034 1102 9 0.15884081785748805 1102 10-11 0.470705148404825 1102 12-13 0.3310060777561432 1102 14-15 -0.10232772502254761 1102 16-17 -0.02301101629565494 1102 18-19 0.4177291676711974 1102 20-21 0.414160106886321 1102 22-23 0.7085205755699846 1102 24-25 0.24817740046940884 1102 26-27 0.2728392564688491 1102 28-29 0.8107970911285065 1102 30-31 0.6176009628715846 1102 32-33 0.9736805540054405 1102 34-35 1.4541412416104151 1102 36-37 1.1548166211206663 1102 38-39 1.3975564656464243 1102 40-41 1.6117135140137258 1102 42-43 2.5028497044575815 1102 44-45 1.7718578704432772 1102 46-47 1.106503443553816 1102 48-49 1.1851873395607342 1102 50-51 2.1978343123297748 1102 52-53 2.4988790955225326 1102 54-55 1.8550792802734044 1102 56-57 1.8406794698726223 1102 58-59 1.2931354459236033 1102 60-61 0.7470298194500309 1102 62-63 0.8775642941092663 1102 64-65 1.1204139873380825 1102 66-67 1.5513012389150305 1102 68-69 1.6743971202396928 1102 70-71 2.5867841233683144 1102 72-73 3.1177978045207944 1102 74-75 2.1724981102070586 1102 76-77 1.5678121518587211 1102 78-79 1.8478689047872656 1102 80-81 2.4763881581888434 1102 82-83 1.2320343110017085 1102 84-85 1.894481832875421 1102 86-87 2.536098867951228 1102 88-89 2.5721349319195497 1102 90-91 2.79844247415501 1102 92-93 3.119166608696286 1102 94-95 3.1248698165488804 1102 96-97 3.5088641546727786 1102 98-99 2.6666349563171963 1102 100-101 3.113924843396777 1102 102-103 3.4977453228887114 1102 104-105 3.457453477183968 1102 106-107 2.844301342095001 1102 108-109 3.408534282905663 1102 110-111 3.6161010441781087 1102 112-113 2.8630578339275523 1102 114-115 3.274504752703507 1102 116-117 2.819020763820074 1102 118-119 2.8281617291623746 1102 120-121 2.6915606076341234 1102 122-123 3.059551954866265 1102 124-125 2.685263446836913 1103 1 0.4738320080793237 1103 2 0.1954832153351127 1103 3 -0.5913952267476823 1103 4 0.07492981475041915 1103 5 0.22108879089132927 1103 6 0.3960660725282068 1103 7 0.5705856220071084 1103 8 0.23069572672319083 1103 9 0.4824143914310639 1103 10-11 -0.3066972539975765 1103 12-13 0.01869376544382817 1103 14-15 0.6187683710735499 1103 16-17 -0.452440445725081 1103 18-19 0.26570214064417286 1103 20-21 -0.27315220542599405 1103 22-23 -0.3658037487543382 1103 24-25 0.15696118425319838 1103 26-27 0.7931095267391228 1103 28-29 1.6677790731104807 1103 30-31 1.6960544163250404 1103 32-33 1.7664733467982288 1103 34-35 2.1849970974662654 1103 36-37 1.9528646691687115 1103 38-39 1.8709048139947697 1103 40-41 2.8155423428425586 1103 42-43 3.4994713260792025 1103 44-45 2.937773786359191 1103 46-47 1.229626566676938 1103 48-49 2.505757910131308 1103 50-51 3.204966444461906 1103 52-53 3.0765817982252344 1103 54-55 2.7090582592523837 1103 56-57 3.4923311215242734 1103 58-59 3.5757931035812582 1103 60-61 2.454237026657239 1103 62-63 2.745432161977135 1103 64-65 2.529948521872619 1103 66-67 3.0944694070831993 1103 68-69 2.7378355586781318 1103 70-71 2.113435775019969 1103 72-73 3.040470477193466 1103 74-75 2.450651263360207 1103 76-77 2.3774968365434113 1103 78-79 3.4871331690515284 1103 80-81 2.24177854857923 1103 82-83 2.8014787554461513 1103 84-85 1.3779653163589067 1103 86-87 2.3367745436269054 1103 88-89 2.105918715703332 1103 90-91 1.709853885566421 1103 92-93 1.6439413834710557 1103 94-95 2.4521971438762122 1103 96-97 2.17590619671482 1103 98-99 2.2762445659268096 1103 100-101 2.62330922778116 1103 102-103 2.2060786562220436 1103 104-105 2.676672696403191 1103 106-107 1.782364405158063 1103 108-109 1.9419426913140718 1103 110-111 2.7921520952291594 1103 112-113 2.5259707468404677 1103 114-115 2.5440242722230266 1103 116-117 2.78298472778404 1103 118-119 1.4111196871203333 1103 120-121 2.3601041511776693 1103 122-123 3.054672074986385 1103 124-125 2.7404436270170933 1104 1 -0.6027445684972506 1104 2 -0.025988256136358245 1104 3 0.03998615463369859 1104 4 -0.3665116266910218 1104 5 -1.2946769748744416 1104 6 -1.2706005941384575 1104 7 -1.0653002638787754 1104 8 -0.7783132822858221 1104 9 -1.4087267497100768 1104 10-11 -0.7995651218654487 1104 12-13 0.0742493209993853 1104 14-15 -0.14286826556308796 1104 16-17 -0.5094975027821391 1104 18-19 0.3366480865901238 1104 20-21 0.30605199877820866 1104 22-23 0.749061116110525 1104 24-25 0.7211503734423914 1104 26-27 0.6106770943066877 1104 28-29 -0.3513650710336549 1104 30-31 -0.4634801182094961 1104 32-33 1.4871940675189563 1104 34-35 0.5892763767455449 1104 36-37 1.9791409454449962 1104 38-39 2.6813402494302068 1104 40-41 2.476578378878589 1104 42-43 2.3136605152683956 1104 44-45 1.9069930055784106 1104 46-47 1.4983953354457036 1104 48-49 0.3473495017228956 1104 50-51 1.2518883663838274 1104 52-53 2.3232034198468554 1104 54-55 1.9631873883815132 1104 56-57 2.0839227131158644 1104 58-59 1.630973283761442 1104 60-61 1.0308136032338133 1104 62-63 1.5532399697849435 1104 64-65 1.9177112846353808 1104 66-67 2.3350850226988165 1104 68-69 1.7554782013207735 1104 70-71 1.9111084476926372 1104 72-73 2.252932939655931 1104 74-75 2.5103359480448972 1104 76-77 2.1488932329398054 1104 78-79 3.010031066949427 1104 80-81 2.395307077107759 1104 82-83 2.4617640407314383 1104 84-85 2.2728602112538 1104 86-87 2.9820448138971756 1104 88-89 2.207270067054683 1104 90-91 3.6362803119928486 1104 92-93 3.0921395816692545 1104 94-95 2.1518968435759085 1104 96-97 2.3467019925106136 1104 98-99 2.626094415776656 1104 100-101 4.195005924477858 1104 102-103 2.5653128904562763 1104 104-105 2.3763723961028873 1104 106-107 1.1956526934463518 1104 108-109 1.9761018504732313 1104 110-111 1.345830773907835 1104 112-113 1.606301077170798 1104 114-115 1.2204506986494508 1104 116-117 1.0082099530092634 1104 118-119 0.9227563237569711 1104 120-121 1.28615520222872 1104 122-123 0.8433357386500511 1104 124-125 0.2528310144044852 1105 1 0.15269379669721062 1105 2 0.5376241367443271 1105 3 -0.1327231400268154 1105 4 0.40013306678293503 1105 5 -0.008586005856642487 1105 6 0.4367164790322775 1105 7 0.4086615027659164 1105 8 0.019313612902053023 1105 9 0.583362900916157 1105 10-11 0.39621602513521026 1105 12-13 -0.20623848387866417 1105 14-15 -0.2934945015551804 1105 16-17 -0.1123320446410716 1105 18-19 -0.4995417617948519 1105 20-21 -0.39137713767531324 1105 22-23 -0.9084866755836103 1105 24-25 -2.138770523063876 1105 26-27 -2.956890473260877 1105 28-29 -2.272261577296021 1105 30-31 -2.1701379955990845 1105 32-33 -1.591250230437545 1105 34-35 -0.8932549350540526 1105 36-37 -1.4651570110480847 1105 38-39 -0.7405450505038722 1105 40-41 -1.0066121287021623 1105 42-43 -0.9243091617256738 1105 44-45 -1.3474565659443307 1105 46-47 -1.4356850864395945 1105 48-49 -2.7821824692724917 1105 50-51 -2.2174590297928347 1105 52-53 -2.1764669822625677 1105 54-55 -2.485724938579594 1105 56-57 -2.2163409651965935 1105 58-59 -2.675223156581339 1105 60-61 -2.5782832985460153 1105 62-63 -2.390069193036414 1105 64-65 -2.5740487681002833 1105 66-67 -2.6125089126999974 1105 68-69 -3.133776907446528 1105 70-71 -3.2995046043838308 1105 72-73 -2.256954997061275 1105 74-75 -2.0971129642820685 1105 76-77 -2.6418121065460234 1105 78-79 -3.0436933892140523 1105 80-81 -3.248397603182287 1105 82-83 -3.8289412987543905 1105 84-85 -3.303606499359251 1105 86-87 -3.21505472466578 1105 88-89 -3.0099349428332545 1105 90-91 -3.134319556167995 1105 92-93 -2.638578941732195 1105 94-95 -2.634523723332464 1105 96-97 -2.663185944206589 1105 98-99 -3.2183353798726486 1105 100-101 -3.7361081163922805 1105 102-103 -3.5876205307698257 1105 104-105 -4.129560365927436 1105 106-107 -4.060115269638684 1105 108-109 -4.855144029553136 1105 110-111 -5.380273379025583 1105 112-113 -4.904246055327555 1105 114-115 -4.611124779267488 1105 116-117 -4.883681938882628 1105 118-119 -4.6095442695192315 1105 120-121 -3.9735679355431976 1105 122-123 -4.033065052384888 1105 124-125 -2.764637673795912 1106 1 0.6119272461745631 1106 2 0.4010387708906684 1106 3 0.4840015986491437 1106 4 0.6558821957027945 1106 5 0.13775545755799357 1106 6 0.18654226300439802 1106 7 -0.9000492986278132 1106 8 -0.7312090351815712 1106 9 -0.5183792593625896 1106 10-11 -0.24280836510869364 1106 12-13 -0.9686078218577592 1106 14-15 -0.39035861305343644 1106 16-17 0.12216272887808799 1106 18-19 -1.4235835736415439 1106 20-21 -0.39973950701329386 1106 22-23 -0.48723232018290474 1106 24-25 -0.49661024431822653 1106 26-27 -0.5854619018323035 1106 28-29 -0.8976971173657091 1106 30-31 -0.6495805043098812 1106 32-33 -1.0240028436779554 1106 34-35 -0.0852409977718267 1106 36-37 0.48222974853379696 1106 38-39 -0.41441264632268826 1106 40-41 -2.726124323824113 1106 42-43 -1.6469572453493697 1106 44-45 -1.9296865311011224 1106 46-47 -2.011643274592906 1106 48-49 -2.1124960581226624 1106 50-51 -2.687493872998413 1106 52-53 -2.812703916060478 1106 54-55 -3.9595925343984106 1106 56-57 -3.3330657038725526 1106 58-59 -3.096825944037789 1106 60-61 -3.012429640009426 1106 62-63 -2.8942503777054043 1106 64-65 -2.9514006844765888 1106 66-67 -3.9598956722818777 1106 68-69 -3.6951009492583786 1106 70-71 -3.801246764662576 1106 72-73 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2216 110-111 1.5211203491974103 2216 112-113 1.2696215404912614 2216 114-115 0.6860877642865191 2216 116-117 1.0734609182602277 2216 118-119 1.5131038141044613 2216 120-121 1.609707325780846 2216 122-123 1.578084773399084 2216 124-125 0.4130626746361443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 29.0 6 60.0 7 143.0 8 196.0 9 362.0 10 548.0 11 745.0 12 994.0 13 1237.0 14 1748.0 15 1291.0 16 437.0 17 294.0 18 238.0 19 204.0 20 209.0 21 223.0 22 203.0 23 216.0 24 227.0 25 233.0 26 267.0 27 324.0 28 381.0 29 418.0 30 531.0 31 554.0 32 687.0 33 880.0 34 1038.0 35 1585.0 36 2108.0 37 3038.0 38 1439.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.053351810150694 15.641780703273861 16.304347826086957 14.00051966048848 2 20.065775239084342 25.184992859924705 34.475745380587654 20.273486520403306 3 17.62084036522567 29.17478038859319 38.72517201090484 14.479207235276299 4 10.813968583668702 20.706218356484488 32.2947769267385 36.18503613310831 5 7.374209751450593 42.38763315146791 35.18662856153114 15.051528535550359 6 23.891124670041975 40.027694837509195 15.708165649746853 20.373014842701977 7 16.673157644207883 40.261370028993035 23.69206802544463 19.373404301354448 8 28.967069107274224 32.10870223722359 18.953654420355708 19.97057423514648 9 23.35453719330131 9.775412177073866 21.614955212254966 45.25509541736986 10-11 23.69639534380544 26.545934484400018 26.535116188497987 23.22255398329655 12-13 28.21844303085378 28.38937210610585 29.665931022545326 13.726253840495046 14-15 22.839586308364705 26.47453373144662 36.71945995066857 13.9664200095201 16-17 13.693798952788958 26.40529663767363 38.77709983123459 21.123804578302828 18-19 12.339348305854863 18.010299017698735 52.20476870483361 17.44558397161279 20-21 14.18278592756069 16.04136916352936 56.021463499069625 13.754381409840322 22-23 13.382232030810506 17.274654896360726 56.32004846596564 13.023064606863127 24-25 13.072828768012462 17.22272707603098 56.53857804318664 13.165866112769917 26-27 13.01008265178069 16.510883205677445 57.505733696828074 12.973300445713793 28-29 12.553550564715046 16.952269678480246 57.28720411960708 13.20697563719763 30-31 13.325976892119954 16.15820675927128 56.809035440737375 13.70678090787139 32-33 12.88459041931715 16.703448872733567 56.741962006144796 13.669998701804492 34-35 12.880263100956338 17.175126574062055 55.77264269332295 14.17196763165866 36-37 14.063784672638366 17.110216798649876 54.11527976113203 14.710718767579731 38-39 14.044311740014711 17.49967545112294 53.373144662252805 15.082868146609545 40-41 14.756155610368255 18.356484486563676 51.73092734432472 15.156432558743345 42-43 15.2126876974339 18.44952183132113 50.96066467610022 15.37712579514475 44-45 16.427566807313642 18.762306610407876 48.13588661689928 16.674239965379208 46-47 17.228172671210647 19.437412095639946 44.90100616682895 18.433409066320458 48-49 17.7817206162368 20.453955340141942 40.979314523108876 20.785009520512375 50-51 17.3965726155444 21.380041544054006 40.36480872425135 20.85857711615025 52-53 17.522070278691363 22.158992556690325 39.37164618314004 20.947290981478275 54-55 17.344642548035313 22.485719231435 38.46070624891812 21.708931971611563 56-57 16.9028712378562 22.76218707402038 37.80210744963974 22.532834238483677 58-59 17.022243378916393 22.0702786913623 37.25549593214471 23.651981997576595 60-61 17.278982214721538 22.19914319096456 37.095936648059194 23.425937946254706 62-63 17.114544117010688 21.91354017915098 38.123674758752 22.848240945086328 64-65 16.69082805028453 21.717117078131423 38.959690157301424 22.632364714282623 66-67 17.55631044853625 21.026894865525673 38.86665079948936 22.55014388644871 68-69 17.798260418018955 21.519754208317103 38.64295296204942 22.039032411614524 70-71 18.993011229634117 20.97280221563494 38.47718372027609 21.557002834454856 72-73 18.722142888052275 21.627937167337404 38.575879527456834 21.07404041715349 74-75 17.761478211952053 21.608464234713747 38.759790557791334 21.87026699554286 76-77 17.8155696914622 21.863776018001644 39.10597602665628 21.214678263879875 78-79 17.9626985157298 21.68635596520836 38.822536674023105 21.52840884503873 80-81 17.2292180535722 21.74910208144013 38.39413215630274 22.62754770868493 82-83 16.526028819940283 22.88069583279242 38.20373014842702 22.38954519884028 84-85 16.632048119780173 23.382664762646588 36.57233112640097 23.41295599117227 86-87 16.385390973213898 23.531957246094596 35.65061231554805 24.43203946514345 88-89 16.980397247825525 23.59903068068718 34.30265264615518 25.11791942533212 90-91 17.179825605296752 22.956920613627023 34.48514615834 25.378107622736223 92-93 17.413129083906703 23.672595092820977 32.53927041412437 26.37500540914795 94-95 17.131853390453937 24.033926175948764 31.431476913756544 27.402743519840755 96-97 18.044917564585226 23.35670085248172 30.570340559954996 28.028041022978062 98-99 18.674946448276607 24.09719367332367 29.075881169266722 28.151978709133 100-101 18.802198277727292 24.22649184300489 29.0319788826864 27.93933099658142 102-103 19.591933878575446 24.232982820546106 28.899995672681637 27.275087628196808 104-105 19.77584490890995 24.577004630230643 28.616556320048463 27.030594140810937 106-107 19.73906270284305 24.957808645982084 29.14881647842832 26.154312172746547 108-109 20.641755198303656 25.261267498972238 28.673431854079666 25.423545448644436 110-111 20.353541910078324 25.529014669609246 29.417110216798648 24.700333203513782 112-113 19.849393027935864 26.15497803648324 29.952610737238437 24.04301819834246 114-115 20.24585019585777 25.52427121431818 29.7963512021988 24.43352738762525 116-117 19.587941221027116 26.669119397493883 30.263812842210054 23.47912653926894 118-119 19.271216514476134 25.942355130479942 31.033885835461117 23.75254251958281 120-121 18.683601272259125 25.89090594370037 31.230932340913515 24.19456044312699 122-123 18.968800034618546 25.34510363927474 31.10260071833485 24.583495607771862 124-125 18.439102494753012 25.189865201116472 31.103273687171384 25.267758616959128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 18.5 2 47.0 3 67.0 4 45.5 5 37.0 6 57.0 7 154.0 8 228.0 9 246.0 10 210.5 11 130.0 12 114.0 13 104.0 14 105.5 15 107.5 16 99.0 17 119.5 18 147.0 19 149.5 20 165.0 21 193.0 22 200.0 23 236.5 24 268.5 25 314.5 26 388.0 27 446.5 28 492.0 29 542.5 30 582.0 31 616.0 32 689.0 33 722.0 34 777.5 35 838.5 36 861.0 37 829.0 38 785.0 39 690.5 40 569.5 41 521.5 42 500.0 43 544.5 44 522.0 45 496.0 46 528.5 47 518.0 48 466.5 49 506.5 50 670.5 51 662.0 52 523.0 53 444.0 54 384.5 55 350.0 56 290.0 57 247.0 58 244.5 59 216.0 60 169.0 61 135.5 62 146.0 63 110.0 64 76.0 65 89.5 66 70.0 67 44.5 68 38.0 69 37.5 70 35.0 71 25.5 72 16.5 73 14.5 74 17.0 75 18.5 76 15.5 77 8.5 78 2.5 79 2.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07356441213380069 2 0.0 3 0.0 4 0.0 5 0.06490977541217707 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.006490977541217708 46-47 0.006490977541217708 48-49 0.004327318360811804 50-51 0.004327318360811804 52-53 0.004327318360811804 54-55 0.004327318360811804 56-57 0.002163659180405902 58-59 0.004327318360811804 60-61 0.0 62-63 0.0 64-65 0.002163659180405902 66-67 0.002163659180405902 68-69 0.0 70-71 0.002163659180405902 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.002163659180405902 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.002163659180405902 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.002163659180405902 110-111 0.0 112-113 0.010818295902029512 114-115 0.023800250984464925 116-117 0.023800250984464925 118-119 0.008654636721623608 120-121 0.002163659180405902 122-123 0.0 124-125 0.002163659180405902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 23109.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.26258167813406 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5043486057563 36.440347916396206 2 9.360710122836904 9.03544073737505 3 5.119698735766161 7.412696352070622 4 3.3354254460683226 6.439049720887965 5 1.9635972384111897 4.738413605088927 6 1.3180310230431274 3.816694794236012 7 0.8517887563884157 2.87766670993985 8 0.636600017932395 2.457916828941105 9 0.3765802922980364 1.6357263403868623 >10 1.407692997399803 11.623177117140509 >50 0.07172957948534027 2.5660997879614005 >100 0.026898592307002603 1.795837119736899 >500 0.026898592307002603 9.16093296983859 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 3.5354191007832445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 737 3.1892336319183 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 2.436280237137046 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.7572807131420658 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.5495694318230993 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.4889869747717339 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.4284045177203687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 89 0.38513133411225065 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.35484010558656803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 81 0.3505127872257562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 79 0.3418581505041326 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 57 0.24665714656627288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 53 0.22934787312302568 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 53 0.22934787312302568 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 46 0.199056644597343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 44 0.1904020078757194 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.1904020078757194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 42 0.1817473711540958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 42 0.1817473711540958 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1730927344324722 No Hit GTACATGGGATTGCTTCAAGTCACTCCATTTAGTTTGATGTTGGCTACTG 37 0.16011077935003679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 37 0.16011077935003679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 37 0.16011077935003679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 36 0.15578346098922496 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 34 0.14712882426760138 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.13847418754597773 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 32 0.13847418754597773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 31 0.13414686918516594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 30 0.12981955082435415 No Hit GTTCTAGACCAATCTTTCTCTTGTTTGGTTAGAGTCACAGCAAGCTGAAC 30 0.12981955082435415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 30 0.12981955082435415 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 29 0.12549223246354235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 28 0.12116491410273054 No Hit GTATAAGAACAGAAGCAACAGAAACCAAGGTTACTTGGCATTATCAGAAC 27 0.11683759574191872 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 26 0.11251027738110693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 25 0.10818295902029512 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 25 0.10818295902029512 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 24 0.10385564065948333 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.004327318360811805 0.0 16-17 0.0 0.0 0.0 0.012981955082435416 0.0 18-19 0.0 0.0 0.0 0.021636591804059024 0.0 20-21 0.0 0.0 0.0 0.021636591804059024 0.0 22-23 0.0 0.0 0.0 0.028127569345276732 0.0 24-25 0.0 0.0 0.0 0.03894586524730624 0.0 26-27 0.0 0.0 0.0 0.03894586524730624 0.0 28-29 0.0 0.0 0.0 0.05409147951014756 0.0 30-31 0.0 0.0 0.0 0.08221904885542429 0.0 32-33 0.0 0.0 0.0 0.15145614262841317 0.0 34-35 0.0 0.0 0.0 0.21636591804059024 0.0 36-37 0.0 0.0 0.0 0.3050759444372323 0.0 38-39 0.0 0.0 0.0 0.4500411095244277 0.0 40-41 0.0 0.0 0.0 0.577697001168376 0.0 42-43 0.0 0.0 0.0 0.685879960188671 0.0 44-45 0.0 0.0 0.0 0.7940629192089662 0.0 46-47 0.0 0.0 0.0 0.9238824700333204 0.0 48-49 0.0 0.0 0.0 1.0277381106928036 0.0 50-51 0.0 0.0 0.0 1.1640486390583755 0.0 52-53 0.0 0.0 0.0 1.2505950062746116 0.0 54-55 0.0 0.0 0.0 1.419360422346272 0.0 56-57 0.0 0.0 0.0 1.6054351118611798 0.0 58-59 0.0 0.0 0.0 1.7439092994071572 0.0 60-61 0.0 0.0 0.0 1.8953654420355706 0.0 62-63 0.0 0.0 0.0 2.0987494049937254 0.0 64-65 0.0 0.0 0.0 2.30213336795188 0.0 66-67 0.0 0.0 0.0 2.5163356268120647 0.0 68-69 0.0 0.0 0.0 2.769483750919555 0.0 70-71 0.0 0.0 0.0 3.0788870137175994 0.0 72-73 0.0 0.0 0.0 3.345017092907525 0.0 74-75 0.0 0.0 0.0 3.669565969968411 0.0 76-77 0.0 0.0 0.0 3.9270414124367132 0.0 78-79 0.0 0.0 0.0 4.167207581461769 0.0 80-81 0.0 0.0 0.0 4.45713791163616 0.0 82-83 0.0 0.0 0.0 4.8163053355835395 0.0 84-85 0.0 0.0 0.0 5.2165822839586315 0.0 86-87 0.0 0.0 0.0 5.683932666926306 0.0 88-89 0.0 0.0 0.0 6.097191570383833 0.0 90-91 0.0 0.0 0.0 6.527759747284607 0.0 92-93 0.0 0.0 0.0 6.884763512051581 0.0 94-95 0.0 0.0 0.0 7.330477303215197 0.0 96-97 0.0 0.0 0.0 7.737245229131507 0.0 98-99 0.0 0.0 0.0 8.180795361114718 0.0 100-101 0.0 0.0 0.0 8.71305551949457 0.0 102-103 0.0 0.0 0.0 9.258297632956857 0.0 104-105 0.0 0.0 0.0 9.695356787398849 0.0 106-107 0.0 0.0 0.0 10.335799904798996 0.0 108-109 0.0 0.0 0.0 10.896187632524125 0.0 110-111 0.0 0.0 0.0 11.454411701068848 0.0 112-113 0.0 0.0 0.0 12.003981132891948 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 15 2.6478045E-4 119.22559 1 TTATCAA 15 2.6708128E-4 118.96753 2 GTGCTAA 25 1.674108E-5 95.38048 1 TCTGCGA 25 1.692296E-5 95.174034 8 CTGCGAT 25 1.692296E-5 95.174034 9 TAATCTG 25 1.692296E-5 95.174034 5 ATGGGTA 20 8.3850545E-4 89.22565 5 GGTATCA 275 0.0 82.37405 1 GCTAATC 30 4.181928E-5 79.31168 3 CTAATCT 30 4.181928E-5 79.31168 4 ATCTGCG 30 4.181928E-5 79.31168 7 CATGGGT 35 8.976455E-5 67.981445 4 AATCTGC 35 8.976455E-5 67.981445 6 TGCTAAT 35 8.976455E-5 67.981445 2 GATCAGA 15 0.0041793752 59.612797 108-109 CATAGGT 20 1.7471162E-4 59.548214 106-107 CCATAGG 25 7.304523E-6 59.548214 106-107 AGATCAG 15 0.0041973833 59.54821 106-107 TGTTTCG 20 1.7565154E-4 59.483765 78-79 AACCGGC 20 1.7565154E-4 59.483765 46-47 >>END_MODULE