FastQCFastQC Report
Thu 26 May 2016
SRR936266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519716
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35230.6778702214286264No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16660.32055969029239045No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14450.27803646606993054No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.17182461190342418No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7880.1516212700782735No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.12622278321236985No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6540.12583795765379555No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.11910351037874532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA1600.059.4936032
GGTATCA21250.052.0743981
GTACATG87350.048.5620351
GTCGGGA4500.047.594882
TACATGG86850.046.581062
ATGGGAT21950.045.2639245
CGGGAGT4750.045.089894
GAACAAA12800.043.6906131
TCGGGAG5200.042.3319853
ACATGGG89400.042.0581173
ACATGGA7400.041.8063133
CGTCACT1002.2242784E-741.645521
CATGGGA69800.041.0829734
ATGGGAG19100.040.8045125
GTATCAA28150.039.5215032
TACCTGG3350.039.0704232
TACATGA5050.038.8770072
GGTCGGG6050.038.351251
GTACATA4700.037.974641
TGGGATA5550.036.446536