##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519716 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.353050512202817 31.0 27.0 33.0 12.0 34.0 2 27.53046856360012 31.0 27.0 34.0 12.0 34.0 3 27.897386264806162 31.0 27.0 34.0 12.0 34.0 4 30.535496309522895 35.0 31.0 37.0 7.0 37.0 5 31.5984941775893 35.0 32.0 37.0 12.0 37.0 6 30.93433913906826 35.0 31.0 37.0 8.0 37.0 7 31.041422623124937 35.0 31.0 37.0 8.0 37.0 8 30.567650409069568 35.0 31.0 37.0 8.0 37.0 9 31.402989324939004 38.0 30.0 39.0 8.0 39.0 10-11 31.68675007119273 37.5 31.0 39.0 8.0 39.0 12-13 31.673182084061295 38.0 31.0 39.0 8.0 39.0 14-15 32.67253653918679 39.0 31.0 41.0 8.0 41.0 16-17 32.84569457165067 39.0 31.0 41.0 8.0 41.0 18-19 32.95820698227493 39.0 31.0 41.0 8.0 41.0 20-21 33.184880781041954 39.0 31.0 41.0 9.0 41.0 22-23 33.21343387542427 39.0 31.0 41.0 10.5 41.0 24-25 33.29284936388335 39.0 30.5 41.0 13.0 41.0 26-27 33.430389866773396 39.0 31.0 41.0 13.0 41.0 28-29 33.306677108266825 38.0 30.0 41.0 10.0 41.0 30-31 33.18231207043847 38.0 30.0 41.0 9.0 41.0 32-33 33.004394707878916 38.0 30.0 40.5 8.0 41.0 34-35 32.919986107797335 38.0 30.0 40.0 5.0 41.0 36-37 32.740891179028544 38.0 30.0 40.0 2.0 41.0 38-39 32.45980112215133 38.0 30.0 40.0 2.0 41.0 40-41 32.52633938535662 38.0 30.0 40.0 2.0 41.0 42-43 32.396302018794884 38.0 30.0 40.0 2.0 41.0 44-45 32.305996929092046 38.0 30.0 40.0 2.0 41.0 46-47 32.294720578161915 38.0 30.0 40.0 2.0 41.0 48-49 32.175107173918065 38.0 30.0 40.0 2.0 41.0 50-51 32.05437681349044 38.0 30.0 40.0 2.0 41.0 52-53 31.900863163727884 37.5 30.0 40.0 2.0 41.0 54-55 31.70600096976041 37.0 30.0 40.0 2.0 41.0 56-57 31.51656770236052 37.0 30.0 40.0 2.0 41.0 58-59 31.252226215856354 36.5 30.0 40.0 2.0 41.0 60-61 30.982512564554487 36.0 30.0 40.0 2.0 41.0 62-63 30.707753850179714 36.0 29.0 40.0 2.0 41.0 64-65 30.417183615667017 35.0 29.0 39.0 2.0 41.0 66-67 30.133140022627742 35.0 29.0 39.0 2.0 41.0 68-69 29.845556034449586 35.0 29.0 39.0 2.0 40.5 70-71 29.49274988647646 35.0 28.5 38.0 2.0 40.0 72-73 29.171075164128098 35.0 28.0 37.5 2.0 40.0 74-75 28.83549188402897 34.0 28.0 37.0 2.0 39.5 76-77 28.510620223352753 34.0 28.0 36.5 2.0 39.0 78-79 28.179512464499844 34.0 27.0 36.0 2.0 39.0 80-81 27.855945362467196 34.0 27.0 36.0 2.0 37.5 82-83 27.541750686913623 34.0 26.5 35.0 2.0 37.0 84-85 27.511035834956015 34.0 27.0 35.0 2.0 37.0 86-87 27.35378649108359 34.0 27.0 35.0 2.0 36.5 88-89 27.148011606338848 34.0 26.5 35.0 2.0 36.0 90-91 26.95150332104457 34.0 26.0 35.0 2.0 36.0 92-93 26.792483779602705 34.0 26.0 35.0 2.0 36.0 94-95 26.627305105095857 34.0 25.0 35.0 2.0 35.0 96-97 26.502335891140547 34.0 25.0 35.0 2.0 35.0 98-99 26.37872703553479 34.0 25.0 35.0 2.0 35.0 100-101 26.229592700628807 34.0 24.5 35.0 2.0 35.0 102-103 25.330664247396655 32.5 22.5 34.5 2.0 35.0 104-105 25.872240800745022 33.5 24.0 35.0 2.0 35.0 106-107 25.946448637332697 34.0 24.0 35.0 2.0 35.0 108-109 25.86674837796027 34.0 23.0 35.0 2.0 35.0 110-111 25.732435599442773 34.0 22.5 35.0 2.0 35.0 112-113 25.6500482956076 34.0 20.5 35.0 2.0 35.0 114-115 25.4984347220405 34.0 19.5 35.0 2.0 35.0 116-117 25.347366638702674 34.0 18.5 35.0 2.0 35.0 118-119 25.12885210384133 33.0 13.5 35.0 2.0 35.0 120-121 24.901623194206067 33.0 2.0 35.0 2.0 35.0 122-123 24.584414372464963 33.0 2.0 35.0 2.0 35.0 124-125 22.81611880334644 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7465454487252963 1101 2 0.6458996740372065 1101 3 0.48486247713212904 1101 4 0.8249647069517287 1101 5 0.5216020851410654 1101 6 0.3739501924702502 1101 7 0.8114829142044506 1101 8 0.44779184190516474 1101 9 0.38374347097740014 1101 10-11 0.47783330423795434 1101 12-13 0.5265970832362186 1101 14-15 0.732271801292292 1101 16-17 0.3585089398550565 1101 18-19 0.43283719182277736 1101 20-21 0.2547394637507452 1101 22-23 0.326029470500778 1101 24-25 0.5439828130694053 1101 26-27 0.698703074575775 1101 28-29 0.7161907801225382 1101 30-31 0.7762718513847844 1101 32-33 0.733394410166234 1101 34-35 0.6206315322373186 1101 36-37 0.6087525876089188 1101 38-39 0.6855651619386833 1101 40-41 0.6769483560090919 1101 42-43 0.7544567589539426 1101 44-45 0.7334692545083286 1101 46-47 0.5277774998840243 1101 48-49 0.7547156154045283 1101 50-51 0.6563242861424285 1101 52-53 0.5877194413040705 1101 54-55 0.5056790905229533 1101 56-57 0.4825910852926647 1101 58-59 0.5653104541308558 1101 60-61 0.6112361728860556 1101 62-63 0.5578837124416367 1101 64-65 0.5103438400057954 1101 66-67 0.494706644003184 1101 68-69 0.41747517913764653 1101 70-71 0.5654149001615068 1101 72-73 0.46096208403446326 1101 74-75 0.43352292898628164 1101 76-77 0.6466267000608354 1101 78-79 0.5768739163508485 1101 80-81 0.3819825313398688 1101 82-83 0.4088109494965373 1101 84-85 0.3635607970002681 1101 86-87 0.5301351396805245 1101 88-89 0.48458408693529975 1101 90-91 0.1840769100706936 1101 92-93 0.28783638662446975 1101 94-95 0.29371322238993613 1101 96-97 0.3361128089277585 1101 98-99 0.3347162586134047 1101 100-101 0.4428220809888437 1101 102-103 0.40791967136916085 1101 104-105 0.3439198946151052 1101 106-107 0.24486341688076152 1101 108-109 0.25452438367598873 1101 110-111 0.3571501403493329 1101 112-113 0.46856706857213126 1101 114-115 0.27408049250269073 1101 116-117 0.272338748985149 1101 118-119 0.3369301884965914 1101 120-121 0.5467981524638539 1101 122-123 0.4779961796287715 1101 124-125 0.47923658699081884 1102 1 0.47808267575398844 1102 2 0.6035474386492226 1102 3 0.6115035025389055 1102 4 0.7899668234215191 1102 5 0.48882421092941186 1102 6 0.4463760931880252 1102 7 0.12092145588063374 1102 8 0.20975294149496548 1102 9 0.3174248798458663 1102 10-11 0.5689115466239869 1102 12-13 0.3521992112780694 1102 14-15 0.3943005061041376 1102 16-17 0.10609936183954005 1102 18-19 0.3477139950093573 1102 20-21 0.4100837783647435 1102 22-23 0.684850277073707 1102 24-25 0.6893477442308509 1102 26-27 0.675112810945862 1102 28-29 0.7176502306930672 1102 30-31 0.6649440178276222 1102 32-33 0.7237111802201639 1102 34-35 0.6798607108947792 1102 36-37 0.6711869237530479 1102 38-39 0.808085923136943 1102 40-41 0.7649493426798983 1102 42-43 0.8912522714289324 1102 44-45 0.8602122968042281 1102 46-47 0.6296268985934361 1102 48-49 0.7977949114575438 1102 50-51 0.5323516875743195 1102 52-53 0.6611738854338363 1102 54-55 0.6684622635222865 1102 56-57 0.7005227613844731 1102 58-59 0.6260759766857262 1102 60-61 0.6137904017145033 1102 62-63 0.5984514135900127 1102 64-65 0.5947544412354802 1102 66-67 0.5562076017438322 1102 68-69 0.5362068503570043 1102 70-71 0.4789297017462708 1102 72-73 0.39038086184167753 1102 74-75 0.3647140599120178 1102 76-77 0.26618135827380485 1102 78-79 0.17039218955512325 1102 80-81 0.29231273107980016 1102 82-83 0.18102550776341175 1102 84-85 0.2899046030524559 1102 86-87 0.2771804564962572 1102 88-89 0.2233135046472583 1102 90-91 0.1966135676319709 1102 92-93 0.38812888579463234 1102 94-95 0.35646350636232427 1102 96-97 0.4773171259170752 1102 98-99 0.4456306953735911 1102 100-101 0.47783442960034606 1102 102-103 0.6580308240991002 1102 104-105 0.4951378544600402 1102 106-107 0.626185799386235 1102 108-109 0.6901326936367944 1102 110-111 0.6988983443952925 1102 112-113 0.6215215994923824 1102 114-115 0.6158896021540983 1102 116-117 0.4952357545610582 1102 118-119 0.5704323826210249 1102 120-121 0.6012317760123977 1102 122-123 0.3493467918823683 1102 124-125 0.3122752894577978 1103 1 0.46500096250975886 1103 2 0.576664085064774 1103 3 0.45763503352310764 1103 4 0.676584381137193 1103 5 0.36380446609344474 1103 6 0.4669608440993258 1103 7 0.43069970618440223 1103 8 0.5690512404014036 1103 9 0.5267854509272745 1103 10-11 0.3805040435863276 1103 12-13 0.5897941383740601 1103 14-15 0.5481317636983007 1103 16-17 0.4154356315843728 1103 18-19 0.6193315527250363 1103 20-21 0.6036743008434797 1103 22-23 0.5477854228817236 1103 24-25 0.6156463469039934 1103 26-27 0.3889865958790324 1103 28-29 0.5367358929044883 1103 30-31 0.2726095706830307 1103 32-33 0.4914974803416712 1103 34-35 0.5323310936408276 1103 36-37 0.35971593347358066 1103 38-39 0.3983236205853018 1103 40-41 0.3145658372121005 1103 42-43 0.5240150296306325 1103 44-45 0.5327252068892747 1103 46-47 0.3644097053978115 1103 48-49 0.18663604146968993 1103 50-51 0.27264634128027154 1103 52-53 0.11583974205595027 1103 54-55 0.012001145660796908 1103 56-57 0.04877686223502664 1103 58-59 -0.08991354336287571 1103 60-61 -0.07155361408136329 1103 62-63 -0.27600256575385274 1103 64-65 -0.28710916751300175 1103 66-67 -0.06443182717475793 1103 68-69 -0.14887820432100085 1103 70-71 -0.004802831668069274 1103 72-73 -0.05819675055200335 1103 74-75 0.17520995906146908 1103 76-77 0.03549104843176565 1103 78-79 -0.14887952976443586 1103 80-81 0.009427706778463829 1103 82-83 -0.09426079235809581 1103 84-85 0.1556738922383616 1103 86-87 0.17224196925446122 1103 88-89 0.17855651801299288 1103 90-91 0.11178367698798297 1103 92-93 0.20634045930617262 1103 94-95 0.20195257076086648 1103 96-97 0.23580892421598065 1103 98-99 0.27726951675876776 1103 100-101 0.5135535972795715 1103 102-103 0.47970381923883565 1103 104-105 0.5232651327103426 1103 106-107 0.3929962489609622 1103 108-109 0.424749947585763 1103 110-111 0.4350010175423158 1103 112-113 0.46758022646686825 1103 114-115 0.44206138222700275 1103 116-117 0.5319831725440487 1103 118-119 0.40819741907303 1103 120-121 0.505628039681902 1103 122-123 0.6697333350172414 1103 124-125 0.8103706722038488 1104 1 0.11782863241267094 1104 2 0.23443107535603502 1104 3 0.2856326063386412 1104 4 -0.024738434396788023 1104 5 0.09308844667597072 1104 6 0.0636477373032065 1104 7 -0.24815951711656936 1104 8 0.07776483263441492 1104 9 0.1612150625777602 1104 10-11 1.2538731958500193E-5 1104 12-13 -0.04712940531526044 1104 14-15 -0.008087896495872826 1104 16-17 0.24326330148728914 1104 18-19 -0.01796213659535084 1104 20-21 0.11872284453280457 1104 22-23 0.09151721899822718 1104 24-25 0.24944489059331687 1104 26-27 0.04754848908291365 1104 28-29 0.19515822300157737 1104 30-31 0.24582559010050176 1104 32-33 0.28651568422517215 1104 34-35 0.20579589946606802 1104 36-37 0.37819287522115275 1104 38-39 0.5243855137891842 1104 40-41 0.3610767595422004 1104 42-43 0.3496096898248098 1104 44-45 0.4018696243650055 1104 46-47 0.3845432005434404 1104 48-49 0.4737355560327927 1104 50-51 0.3386657587560009 1104 52-53 0.2666649847743976 1104 54-55 0.3724865825540036 1104 56-57 0.6128484641767749 1104 58-59 0.6007114566371214 1104 60-61 0.4479912888312576 1104 62-63 0.3128384536636233 1104 64-65 0.4259854926811748 1104 66-67 0.4624977844757261 1104 68-69 0.39311208694113375 1104 70-71 0.6391231392057186 1104 72-73 0.5868699970208127 1104 74-75 0.57314078430419 1104 76-77 0.6656670193055518 1104 78-79 0.6426071207209993 1104 80-81 0.6749798912444867 1104 82-83 0.6600899357972416 1104 84-85 0.5194785038888483 1104 86-87 0.594347551778732 1104 88-89 0.6178526345178454 1104 90-91 0.47127396825012013 1104 92-93 0.4461402165877253 1104 94-95 0.509355483382226 1104 96-97 0.3668647494586992 1104 98-99 0.504077769185951 1104 100-101 0.533365490483451 1104 102-103 0.612476877491261 1104 104-105 0.4315296958171366 1104 106-107 0.38331178294154 1104 108-109 0.4199926660323676 1104 110-111 0.45805926996949964 1104 112-113 0.3905777992824042 1104 114-115 0.4057567705765166 1104 116-117 0.4086275900197762 1104 118-119 0.2376330986846824 1104 120-121 0.24562197172073397 1104 122-123 0.36642629618228995 1104 124-125 0.30544348773782914 1105 1 0.2821805653978444 1105 2 0.30244627519714484 1105 3 0.6366046484448873 1105 4 0.5895612162755803 1105 5 0.6973799775254541 1105 6 0.3224948392858451 1105 7 0.20517924890401673 1105 8 0.17116616218239145 1105 9 0.19861005983220892 1105 10-11 0.3725430026717689 1105 12-13 0.47268748975793784 1105 14-15 0.43034821977531834 1105 16-17 0.3126994703328734 1105 18-19 0.31999190170216707 1105 20-21 0.35334337424901463 1105 22-23 0.04255001373611833 1105 24-25 0.24850585352252352 1105 26-27 0.28473494124605736 1105 28-29 0.2985559891740408 1105 30-31 0.4575689569646144 1105 32-33 0.43069949598547197 1105 34-35 0.5673032657226571 1105 36-37 0.4095542307058295 1105 38-39 0.5088643847248946 1105 40-41 0.6297771789690216 1105 42-43 0.7426266421456731 1105 44-45 0.9078718675390931 1105 46-47 0.8088185501631528 1105 48-49 0.7867247899654757 1105 50-51 0.727565113843454 1105 52-53 0.593661643379658 1105 54-55 0.6033940457811369 1105 56-57 0.6115348345575278 1105 58-59 0.6878053194530054 1105 60-61 0.5909892559547387 1105 62-63 0.7365252621643172 1105 64-65 0.6139783776133534 1105 66-67 0.3639872462307743 1105 68-69 0.25307727100992494 1105 70-71 0.23463985786261787 1105 72-73 0.27215749132525247 1105 74-75 0.2737982562937198 1105 76-77 0.34669817839566264 1105 78-79 0.3164100310057165 1105 80-81 0.3808108295221473 1105 82-83 0.5143500680510478 1105 84-85 0.5394246443442583 1105 86-87 0.5332159463904418 1105 88-89 0.5702472520683486 1105 90-91 0.6172650247617497 1105 92-93 0.7382019214000373 1105 94-95 0.6408966140821661 1105 96-97 0.4916347364903473 1105 98-99 0.5308089271077456 1105 100-101 0.6722517019727086 1105 102-103 0.585477736206343 1105 104-105 0.5561426898703914 1105 106-107 0.6778999794062059 1105 108-109 0.7018498272488216 1105 110-111 0.7974408490705933 1105 112-113 0.6601719833862418 1105 114-115 0.7456180910116004 1105 116-117 0.7570636478749755 1105 118-119 0.8287803011428281 1105 120-121 0.8537229554460382 1105 122-123 0.9830268067380601 1105 124-125 1.0914145951882084 1106 1 -0.25544686619953083 1106 2 -0.4542466507615224 1106 3 -0.34640447190511736 1106 4 -0.45961435227655656 1106 5 -0.29949619735649335 1106 6 -0.44772401717932553 1106 7 -0.4601838885079985 1106 8 -0.6147937535672341 1106 9 -0.5104416298929912 1106 10-11 -0.4360784353039051 1106 12-13 -0.420756977428109 1106 14-15 -0.5030556609096593 1106 16-17 -0.4902353575591718 1106 18-19 -0.6304558417261106 1106 20-21 -0.6508190646571208 1106 22-23 -0.5852807713281649 1106 24-25 -4.288191107860783 1106 26-27 -1.3890343631076973 1106 28-29 -0.8929655546226556 1106 30-31 -0.7049251096547309 1106 32-33 -4.875196578765689 1106 34-35 -1.1546102513531409 1106 36-37 -0.48811654541665206 1106 38-39 -0.35067743490323977 1106 40-41 -0.7805826851039441 1106 42-43 -0.4946113274260604 1106 44-45 -0.34852856271265154 1106 46-47 -0.4852027795237852 1106 48-49 -0.33326594888012906 1106 50-51 -0.28318900250742374 1106 52-53 -0.39425675975346053 1106 54-55 -0.501988601021953 1106 56-57 -0.3639666540496549 1106 58-59 -0.40155030452089946 1106 60-61 -0.36052903627678035 1106 62-63 -0.3696673727502322 1106 64-65 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-1.4123397427825424 2216 70-71 -1.6141095934546676 2216 72-73 -1.5946313039018776 2216 74-75 -1.5318635521047845 2216 76-77 -1.3113560359354821 2216 78-79 -1.1060181774071154 2216 80-81 -1.1294013875143385 2216 82-83 -1.0965607303233575 2216 84-85 -1.191325177240543 2216 86-87 -1.0954953136239496 2216 88-89 -1.1218529112674034 2216 90-91 -1.260511608371818 2216 92-93 -1.0803856573191375 2216 94-95 -1.1723340297354135 2216 96-97 -1.180602366106605 2216 98-99 -0.9950971085514055 2216 100-101 -1.0710230156236555 2216 102-103 -1.1408281286519824 2216 104-105 -1.0079352394980923 2216 106-107 -1.0522518899968603 2216 108-109 -1.0721235015457502 2216 110-111 -1.1774369477182312 2216 112-113 -1.1538372673296564 2216 114-115 -1.036045007351163 2216 116-117 -1.22907296889516 2216 118-119 -1.3745398638053814 2216 120-121 -1.4336539082089175 2216 122-123 -1.531235957786727 2216 124-125 -1.5454171689872176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28912.0 3 5255.0 4 4993.0 5 4656.0 6 5275.0 7 4069.0 8 3062.0 9 3202.0 10 3963.0 11 4717.0 12 4570.0 13 4305.0 14 4215.0 15 4067.0 16 3830.0 17 3484.0 18 3011.0 19 2569.0 20 2215.0 21 2272.0 22 2735.0 23 4541.0 24 6898.0 25 9585.0 26 11376.0 27 8034.0 28 7894.0 29 9938.0 30 11268.0 31 13324.0 32 17511.0 33 22892.0 34 31406.0 35 44892.0 36 72193.0 37 102577.0 38 39864.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.43326137067695 19.964249568031647 9.15080217196381 18.451686889327593 2 17.62235071144762 25.97000278999067 36.55849840778119 19.849148090780524 3 16.727982205666173 37.63709410524209 27.11519368270363 18.519730006388105 4 9.58646327196601 20.69126747121483 32.72158426205283 37.00068499476633 5 9.398318292894114 51.16391833907371 28.771045390698657 10.666717977333514 6 25.32032102333403 39.805238660568435 20.211924658417242 14.662515657680297 7 27.132579452811274 41.36337310543745 17.999426604401293 13.50462083734999 8 26.87152450405034 38.74218314059764 18.19803351869312 16.188258836658907 9 28.51903661472093 21.055436792512733 15.93622584946576 34.489300743300575 10-11 22.31000647478346 35.10573715842631 24.507199709067677 18.07705665772255 12-13 23.48936661487471 34.03289350259521 24.527739160970356 17.95000072155972 14-15 21.90841141456347 33.79737908753917 23.303050098661835 20.991159399235528 16-17 20.724333300301804 35.6537500565224 23.762881093234533 19.859035549941265 18-19 20.394454546329168 33.62407519650571 26.21025389403604 19.77121636312908 20-21 23.129845315497505 31.680017240616277 25.91749518474975 19.272642259136468 22-23 22.84256432033109 32.55029065596675 24.772246471637814 19.834898552064345 24-25 21.33910455055796 32.09327205503886 26.121909427550793 20.44571396685238 26-27 21.466992617991398 32.12765977916509 25.88763530395346 20.517712298890054 28-29 21.15958900155856 31.862480999018683 26.238671566835354 20.739258432587405 30-31 21.162682408718673 31.931435102518318 26.42165276152946 20.484229727233544 32-33 20.457698442782405 32.26180420196687 26.228331624043456 21.052165731207275 34-35 21.02999254395459 32.05416456213772 26.827332419366478 20.088510474541213 36-37 21.248073455926637 32.11878479083648 25.695239487948157 20.937902265288727 38-39 21.213596389535347 31.936564805666222 26.145755866475916 20.704082938322514 40-41 21.07232879875315 32.25067826287737 26.310923399588233 20.366069538781243 42-43 20.71097342748841 32.32485424563699 26.16776663908718 20.79640568778742 44-45 20.839141866754407 32.33171608369676 26.216376648877883 20.612765400670952 46-47 20.93833189502982 32.105339617613296 25.956904770838324 20.999423716518557 48-49 20.619397765503944 32.19661949012391 26.132713249293605 21.051269495078547 50-51 20.882298895250337 32.252571383491386 26.28424863457636 20.580881086681913 52-53 21.00850280251909 32.62813371277499 25.931238707666378 20.432124777039547 54-55 20.374535798810875 32.659367724307785 26.236555001827945 20.729541475053395 56-57 20.78378250968568 32.71706074824349 25.479281253697973 21.019875488372854 58-59 20.809217412720894 32.06776060587402 25.919836370913117 21.203185610491964 60-61 20.797423952927684 32.72010790612475 25.859756672458346 20.622711468489218 62-63 20.65561437029426 32.58705318917629 25.916422781122584 20.84090965940686 64-65 19.92415015268101 32.72703483282055 26.227172409281312 21.12164260521713 66-67 20.173171834563174 33.14075983952743 25.351105894576836 21.334962431332556 68-69 20.552092643350615 32.52984577079487 25.76631163131225 21.15174995454226 70-71 19.95551373744254 32.4463980943255 26.128656118496625 21.469432049735335 72-73 20.237284352547746 32.83161508060202 25.66950284194769 21.261597724902543 74-75 19.97800702889969 33.224009859316176 25.8181702733151 20.979812838469037 76-77 19.53701191623806 33.11638583751354 26.289418542228276 21.05718370402012 78-79 20.295681855789915 32.584171294211814 26.003532712222494 21.116614137775777 80-81 19.89074707794613 33.16262965125844 25.873778048013175 21.072845222782256 82-83 19.813282100076098 32.93471704176043 25.717389066497088 21.534611791666386 84-85 19.864540491136925 32.52567525314477 26.134256920893762 21.475527334824545 86-87 19.805565577121968 32.90463477403372 25.963200808139113 21.326598840705195 88-89 20.350289823700603 32.15585540082257 26.307333381436855 21.18652139403997 90-91 19.912278760742677 32.82735040103749 25.873724900883083 21.38664593733675 92-93 19.740663810594917 32.84476098758638 25.785748439278333 21.62882676254037 94-95 19.639070719665046 32.7069311198082 26.11503634691388 21.53896181361287 96-97 19.604281593879698 32.85977068112593 25.94008616293993 21.59586156205444 98-99 19.437864983692986 32.9596028592594 25.613076397641017 21.9894557594066 100-101 19.13991322166957 33.34298605966732 25.90814196242171 21.6089587562414 102-103 19.28225494553704 33.66447891231858 25.748925845325576 21.304340296818808 104-105 18.790971975024775 33.89703972369472 25.8008716315673 21.51111666971321 106-107 18.714734175656396 34.41165180431142 25.49164106762668 21.38197295240551 108-109 18.809780820494467 34.645120076350125 24.885080361970402 21.660018741185006 110-111 18.539167478817532 35.125132164926434 25.1544850450395 21.181215311216533 112-113 18.80712894148207 34.90987805757991 25.067994323784788 21.214998677153236 114-115 18.158081327500632 35.338220000057724 24.932390634455327 21.571308037986313 116-117 18.4333823348316 35.50004617942245 24.833273043531804 21.23329844221415 118-119 18.438124448609173 35.03646755825621 24.93041772455136 21.594990268583253 120-121 18.505117247403856 34.71954713466855 25.007840866016434 21.76749475191115 122-123 18.635784207614787 34.605960025975904 24.971498665127353 21.78675710128196 124-125 18.48343512008528 34.70187449250935 24.62123398638482 22.193456401020562 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2586.0 1 3126.0 2 3657.5 3 3164.5 4 2305.5 5 1659.0 6 1276.0 7 1145.5 8 1094.5 9 1006.5 10 913.5 11 854.0 12 824.5 13 855.5 14 883.0 15 899.0 16 949.0 17 976.5 18 1063.5 19 1228.5 20 1459.0 21 1740.0 22 2065.0 23 2594.5 24 3176.5 25 3866.5 26 4807.5 27 5594.5 28 6394.5 29 7267.0 30 8443.0 31 9833.0 32 11105.0 33 12609.5 34 14143.0 35 14981.0 36 16038.0 37 17172.5 38 16943.5 39 16360.0 40 15640.0 41 15205.0 42 15116.5 43 15471.0 44 15849.5 45 15440.0 46 15731.5 47 15785.0 48 15233.0 49 14990.0 50 15131.0 51 14215.0 52 12742.5 53 12459.0 54 11997.5 55 11373.5 56 10605.5 57 9834.5 58 9329.0 59 8944.5 60 8942.5 61 8604.5 62 7074.0 63 4573.0 64 2998.5 65 2430.0 66 1847.0 67 1427.5 68 1042.0 69 745.5 70 587.0 71 475.5 72 431.5 73 493.5 74 420.5 75 246.5 76 153.0 77 116.0 78 91.0 79 51.5 80 24.5 81 14.0 82 13.5 83 12.5 84 7.0 85 3.5 86 5.5 87 4.5 88 2.0 89 3.5 90 3.0 91 2.0 92 1.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.848255585742983E-4 2 1.9241277928714915E-4 3 0.0 4 7.696511171485966E-4 5 0.0011544766757228947 6 5.772383378614474E-4 7 9.620638964357457E-4 8 0.0011544766757228947 9 0.001346889455010044 10-11 0.0014430958446536185 12-13 0.0016355086239407677 14-15 0.002212746961802215 16-17 0.002020334182515066 18-19 0.0021165405721586404 20-21 0.002501366130732939 22-23 0.08841367208244502 24-25 0.002020334182515066 26-27 0.0012506830653664694 28-29 0.0011544766757228947 30-31 7.696511171485966E-4 32-33 0.001346889455010044 34-35 6.73444727505022E-4 36-37 5.772383378614474E-4 38-39 8.658575067921711E-4 40-41 0.0011544766757228947 42-43 0.0011544766757228947 44-45 0.0012506830653664694 46-47 0.0012506830653664694 48-49 2.886191689307237E-4 50-51 0.0010582702860793202 52-53 9.620638964357457E-4 54-55 0.0011544766757228947 56-57 0.0010582702860793202 58-59 7.696511171485966E-4 60-61 9.620638964357457E-4 62-63 9.620638964357457E-4 64-65 9.620638964357457E-4 66-67 1.9241277928714915E-4 68-69 4.8103194821787286E-4 70-71 9.620638964357457E-4 72-73 3.848255585742983E-4 74-75 8.658575067921711E-4 76-77 9.620638964357457E-4 78-79 3.848255585742983E-4 80-81 8.658575067921711E-4 82-83 2.886191689307237E-4 84-85 6.73444727505022E-4 86-87 6.73444727505022E-4 88-89 6.73444727505022E-4 90-91 2.886191689307237E-4 92-93 8.658575067921711E-4 94-95 3.848255585742983E-4 96-97 5.772383378614474E-4 98-99 1.9241277928714915E-4 100-101 1.9241277928714915E-4 102-103 9.620638964357457E-4 104-105 1.9241277928714915E-4 106-107 4.8103194821787286E-4 108-109 9.620638964357457E-4 110-111 0.0010582702860793202 112-113 6.73444727505022E-4 114-115 4.8103194821787286E-4 116-117 7.696511171485966E-4 118-119 0.0014430958446536185 120-121 5.772383378614474E-4 122-123 6.73444727505022E-4 124-125 3.848255585742983E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 519716.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.849703421299544 #Duplication Level Percentage of deduplicated Percentage of total 1 65.1872171007312 25.32504051223274 2 11.10527770624478 8.628734905975588 3 6.945928785958218 8.095418199598319 4 4.770465122743548 7.413246208009608 5 3.2328486591727574 6.279760580740376 6 2.1593717801700265 5.03345719415575 7 1.462277854407369 3.976634267428237 8 1.0201982405915806 3.170751926233161 9 0.7529735982143687 2.6327520877227295 >10 3.174455453395586 20.516593832693804 >50 0.1304546946771558 3.4361124805338985 >100 0.054066774598182286 3.4189617872252582 >500 0.0029761527301751718 0.794134907290908 >1k 0.0014880763650875859 1.2784011101596418 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3523 0.6778702214286264 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1666 0.32055969029239045 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1445 0.27803646606993054 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 893 0.17182461190342418 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 788 0.1516212700782735 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 656 0.12622278321236985 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 654 0.12583795765379555 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 619 0.11910351037874532 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9241277928714915E-4 0.0 20-21 0.0 0.0 0.0 2.8861916893072373E-4 0.0 22-23 0.0 0.0 0.0 5.772383378614475E-4 0.0 24-25 0.0 0.0 0.0 0.0019241277928714914 0.0 26-27 0.0 0.0 0.0 0.0032710172478815354 0.0 28-29 0.0 0.0 0.0 0.00442549392360443 0.0 30-31 0.0 0.0 0.0 0.012314417874377544 0.0 32-33 0.0 0.0 0.0 0.026168137983052283 0.0 34-35 0.0 0.0 0.0 0.040502890039944894 0.0 36-37 0.0 0.0 0.0 0.06551655134727427 0.0 38-39 0.0 0.0 0.0 0.10659667972508062 0.0 40-41 0.0 0.0 0.0 0.15748985984653158 0.0 42-43 0.0 0.0 0.0 0.2171378214255478 0.0 44-45 0.0 0.0 0.0 0.29112053506145663 0.0 46-47 0.0 0.0 0.0 0.3768404282338816 0.0 48-49 0.0 0.0 0.0 0.46063619361343505 0.0 50-51 0.0 0.0 0.0 0.5486650401373058 0.0 52-53 0.0 0.0 0.0 0.6524717345627227 0.0 54-55 0.0 0.0 0.0 0.7662838935110714 0.0 56-57 0.0 0.0 0.0 0.9110745099246511 0.0 58-59 0.0 0.0 0.0 1.0941552694163734 0.0 60-61 0.0 0.0 0.0 1.2563592423554404 0.0 62-63 0.0 0.0 0.0 1.4225076772698935 0.0 64-65 0.0 0.0 0.0 1.6052998175926851 0.0 66-67 0.0 0.0 0.0 1.8252276243178966 0.0 68-69 0.0 0.0 0.0 2.112596110183254 0.0 70-71 0.0 0.0 0.0 2.4187248420291083 0.0 72-73 0.0 0.0 0.0 2.7033033425948014 0.0 74-75 0.0 0.0 0.0 3.0045255485688336 0.0 76-77 0.0 0.0 0.0 3.353369917416435 0.0 78-79 0.0 0.0 0.0 3.7765818254585195 0.0 80-81 0.0 0.0 0.0 4.215860200571081 0.0 82-83 0.0 0.0 0.0 4.661007165451901 0.0 84-85 0.0 0.0 0.0 5.108174464515235 0.0 86-87 0.0 0.0 0.0 5.60325254562107 0.0 88-89 0.0 0.0 0.0 6.149897251575861 0.0 90-91 0.0 0.0 0.0 6.7465692801453105 0.0 92-93 0.0 0.0 0.0 7.363829476098484 0.0 94-95 0.0 0.0 0.0 8.02524840489806 0.0 96-97 0.0 0.0 0.0 8.697827274896289 0.0 98-99 0.0 0.0 0.0 9.418990371664524 0.0 100-101 0.0 0.0 0.0 10.219427533499065 0.0 102-103 0.0 0.0 0.0 10.989848301764809 0.0 104-105 0.0 0.0 0.0 11.804042977318382 0.0 106-107 0.0 0.0 0.0 12.6647053390698 0.0 108-109 0.0 0.0 0.0 13.52652217749694 0.0 110-111 0.0 0.0 0.0 14.485218850295162 0.0 112-113 0.0 0.0 0.0 15.405048141677378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 160 0.0 59.493603 2 GGTATCA 2125 0.0 52.074398 1 GTACATG 8735 0.0 48.562035 1 GTCGGGA 450 0.0 47.59488 2 TACATGG 8685 0.0 46.58106 2 ATGGGAT 2195 0.0 45.263924 5 CGGGAGT 475 0.0 45.08989 4 GAACAAA 1280 0.0 43.690613 1 TCGGGAG 520 0.0 42.331985 3 ACATGGG 8940 0.0 42.058117 3 ACATGGA 740 0.0 41.806313 3 CGTCACT 100 2.2242784E-7 41.64552 1 CATGGGA 6980 0.0 41.082973 4 ATGGGAG 1910 0.0 40.804512 5 GTATCAA 2815 0.0 39.521503 2 TACCTGG 335 0.0 39.070423 2 TACATGA 505 0.0 38.877007 2 GGTCGGG 605 0.0 38.35125 1 GTACATA 470 0.0 37.97464 1 TGGGATA 555 0.0 36.44653 6 >>END_MODULE