FastQCFastQC Report
Thu 26 May 2016
SRR936266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519716
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98921.9033472127084794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84701.6297362405621532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77781.496586597295446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.4173433182738264No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.3919448314079228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.38078489020926815No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15740.3028577145979727No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.26841582710557305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.2651448098576915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7310.140653741658906No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7260.13969167776247027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7190.13834478830746022No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7080.1362282477353016No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT5330.10255601136005049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.077.1902161
TATAACG950.075.185492
TAACGCA1300.068.679064
ATAACGC1300.059.521853
ATAGGCG300.00422909459.5218475
GTATCAA69500.049.6729132
CCGAGTA505.320247E-447.617484
CAACGTA255.3277746E-447.6083246-47
TGCGCCG558.508697E-443.288625
CACGCAG1650.043.2886165
TTGTGCG558.5288804E-443.2677969
GTACATG69350.042.8530161
ACATGGG70000.041.7503283
TACATGG71400.041.348512
TACCTGG2900.041.0495532
ATGGGAG14900.040.3470235
CCACGCA1351.3187673E-939.6812324
CATGGGG13900.038.9675454
CATGGGA53950.038.835394
ATGGGAT19600.037.960365
Produced by FastQC (version 0.11.5)