FastQCFastQC Report
Thu 26 May 2016
SRR936265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444870
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.068.0725561
GTACATG131400.054.7401961
TACATGG133800.053.4915162
ACATGGG135400.051.4972883
GTATCAA29600.049.0426032
CATGGGA99200.048.2791524
ATGGGAG38600.044.2354055
ATGGGAT39350.041.5779765
CATGGGG36700.040.8516774
TCAACGC36600.039.8254285
CAACGCA37200.039.183086
ATCAACG37200.039.183084
AACGCAG39200.037.032177
GAGTACA75700.033.244511
TATCAAC44800.032.801553
AGTACAT74450.032.763822
ATGGGGG20200.031.5143095
ATGGGAC19450.031.20015
ATGGGCG3150.030.2193325
ACGCAGA49500.029.4466788
Produced by FastQC (version 0.11.5)