##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936265_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1444870 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.05914096077848 31.0 30.0 33.0 26.0 34.0 2 30.046213154124594 31.0 30.0 34.0 26.0 34.0 3 30.181446081654403 31.0 30.0 34.0 26.0 34.0 4 33.784106528615034 35.0 35.0 37.0 28.0 37.0 5 33.91275962543343 35.0 35.0 37.0 30.0 37.0 6 34.023447784229724 37.0 35.0 37.0 30.0 37.0 7 33.85448241018223 37.0 35.0 37.0 30.0 37.0 8 33.841305446164704 37.0 35.0 37.0 29.0 37.0 9 35.33347498390859 39.0 35.0 39.0 29.0 39.0 10-11 35.14545599258065 39.0 35.0 39.0 28.5 39.0 12-13 35.16937163897098 39.0 35.0 39.0 28.0 39.0 14-15 36.30109179372539 40.0 36.0 41.0 28.5 41.0 16-17 36.29937952895416 40.0 36.0 41.0 28.5 41.0 18-19 36.333330334216924 40.0 36.0 41.0 28.5 41.0 20-21 36.44957920089696 40.0 36.0 41.0 29.0 41.0 22-23 36.30790901603605 39.5 36.0 41.0 28.5 41.0 24-25 36.26980281963083 39.0 36.0 41.0 28.5 41.0 26-27 36.3687878494259 39.0 36.0 41.0 29.0 41.0 28-29 36.26696242568535 39.0 36.0 41.0 29.0 41.0 30-31 36.193505990158286 39.0 36.0 41.0 29.0 41.0 32-33 36.03965685494197 39.0 35.0 41.0 28.5 41.0 34-35 35.99802715815264 39.0 35.0 41.0 28.0 41.0 36-37 35.882272107525246 39.0 35.0 41.0 28.0 41.0 38-39 35.635284835313904 39.0 35.0 41.0 27.0 41.0 40-41 35.736665928422624 39.0 35.0 40.5 27.5 41.0 42-43 35.65132987742842 39.0 35.0 41.0 27.0 41.0 44-45 35.49800431872764 39.0 35.0 40.5 27.0 41.0 46-47 35.472446656100544 39.0 35.0 40.0 27.0 41.0 48-49 35.37603936686345 39.0 34.5 40.0 27.0 41.0 50-51 35.23199941863282 39.0 34.0 40.0 27.0 41.0 52-53 35.07609508121838 38.5 34.0 40.0 26.5 41.0 54-55 34.906488126959516 38.0 34.0 40.0 26.0 41.0 56-57 34.71491414452511 38.0 34.0 40.0 26.0 41.0 58-59 34.476719358835055 38.0 33.0 40.0 26.0 41.0 60-61 34.24140822357721 38.0 33.0 40.0 25.0 41.0 62-63 33.992127319412816 37.0 33.0 40.0 24.5 41.0 64-65 33.71165710409933 37.0 32.5 40.0 23.5 41.0 66-67 33.41907818696492 36.0 32.0 39.5 23.0 41.0 68-69 33.12597742357444 36.0 32.0 39.0 23.0 41.0 70-71 32.77926837708583 35.5 32.0 39.0 22.0 40.0 72-73 32.44644985362005 35.0 31.5 38.5 22.0 40.0 74-75 32.10375431699738 35.0 31.0 37.5 21.5 39.5 76-77 31.773267145141084 35.0 31.0 37.0 20.5 39.0 78-79 31.42304567192896 35.0 31.0 36.5 20.0 39.0 80-81 31.09786312955491 35.0 31.0 36.0 19.5 38.5 82-83 30.784124523313515 34.0 31.0 36.0 18.0 37.0 84-85 30.813213991570173 34.5 31.0 35.0 19.5 37.0 86-87 30.691221701606374 34.5 31.0 35.0 19.0 36.5 88-89 30.514787489531933 34.0 31.0 35.0 18.0 36.0 90-91 30.347510156623088 34.0 31.0 35.0 18.0 36.0 92-93 30.216825389135355 34.0 31.0 35.0 16.5 36.0 94-95 30.089740945552194 34.0 31.0 35.0 13.5 35.0 96-97 29.982345816578654 34.0 31.0 35.0 10.5 35.0 98-99 29.866655823707326 34.0 31.0 35.0 8.5 35.0 100-101 29.75356052793677 34.0 30.5 35.0 7.0 35.0 102-103 28.73958418404424 33.5 29.0 34.5 5.5 35.0 104-105 29.425287396097918 34.0 30.0 35.0 2.0 35.0 106-107 29.547709482513998 34.0 30.0 35.0 2.0 35.0 108-109 29.49127430149425 34.0 30.0 35.0 2.0 35.0 110-111 29.3689304228062 34.0 30.0 35.0 2.0 35.0 112-113 29.310784707274703 34.0 30.0 35.0 2.0 35.0 114-115 29.17659166568619 34.0 30.0 35.0 2.0 35.0 116-117 29.03833320644764 34.0 30.0 35.0 2.0 35.0 118-119 28.822614837321005 34.0 30.0 35.0 2.0 35.0 120-121 28.603028992227674 34.0 29.5 35.0 2.0 35.0 122-123 28.2811207928741 34.0 29.0 35.0 2.0 35.0 124-125 26.190572162201445 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29520955430228923 1101 2 0.32979382458988127 1101 3 0.3233281074327117 1101 4 0.2944809500798229 1101 5 0.3077816809693985 1101 6 0.10000515608148675 1101 7 0.18362250456014806 1101 8 0.19798001417827749 1101 9 0.39290595173983434 1101 10-11 0.38165322336062246 1101 12-13 0.3110116187090597 1101 14-15 0.3092479972403268 1101 16-17 0.2980541808356918 1101 18-19 0.24250424842473706 1101 20-21 0.29231946585977653 1101 22-23 0.42411005397747203 1101 24-25 0.5267943713955177 1101 26-27 0.41544271440604774 1101 28-29 0.36773828989736757 1101 30-31 0.49319080164859486 1101 32-33 0.5633532975811306 1101 34-35 0.4832630592386167 1101 36-37 0.5406359835547647 1101 38-39 0.6640438948093816 1101 40-41 0.5722828741775103 1101 42-43 0.6573808385761382 1101 44-45 0.6655496393902993 1101 46-47 0.5481461410905055 1101 48-49 0.6079289502851992 1101 50-51 0.5979436944946741 1101 52-53 0.6195340476712232 1101 54-55 0.5139237989507421 1101 56-57 0.4868066470578043 1101 58-59 0.6773418143384404 1101 60-61 0.6041155500980722 1101 62-63 0.5532998533192597 1101 64-65 0.42987431422414346 1101 66-67 0.5322170715376089 1101 68-69 0.4529430958715679 1101 70-71 0.40095074432826294 1101 72-73 0.4040436331002297 1101 74-75 0.27743062543901686 1101 76-77 0.33434399958982297 1101 78-79 0.3129957792276521 1101 80-81 0.387428765283218 1101 82-83 0.29735312849287965 1101 84-85 0.1339872947090015 1101 86-87 0.22781773410364892 1101 88-89 0.2342220720909367 1101 90-91 0.14926589601969198 1101 92-93 0.20622905073454234 1101 94-95 0.2045388224034852 1101 96-97 0.21202895223326124 1101 98-99 0.18438926267135614 1101 100-101 0.18776645636070555 1101 102-103 0.23824387514334688 1101 104-105 0.2347380287547658 1101 106-107 0.18249098528653107 1101 108-109 0.1876562874349581 1101 110-111 0.22362493411366202 1101 112-113 0.25414193300739285 1101 114-115 0.23357358303609388 1101 116-117 0.27672908202316293 1101 118-119 0.3351107454543012 1101 120-121 0.3080668512682436 1101 122-123 0.429007759417221 1101 124-125 0.418391194008116 1102 1 0.39812699891170666 1102 2 0.3831532628143215 1102 3 0.43139789606593126 1102 4 0.5188008741228742 1102 5 0.49642589403119075 1102 6 0.5247447709440678 1102 7 0.3395250190410337 1102 8 0.3361609663164771 1102 9 0.5317253213991151 1102 10-11 0.5802588207250707 1102 12-13 0.599896144392325 1102 14-15 0.5812293935227686 1102 16-17 0.4701216420128915 1102 18-19 0.5173601166088417 1102 20-21 0.42724182027031077 1102 22-23 0.6905938111701246 1102 24-25 0.7506903431920335 1102 26-27 0.5778751687968295 1102 28-29 0.5758076404399617 1102 30-31 0.5450647143873084 1102 32-33 0.8129582194894809 1102 34-35 0.6639925677487284 1102 36-37 0.761769395691303 1102 38-39 0.7279202959028552 1102 40-41 0.5501206659458688 1102 42-43 0.7652700143922573 1102 44-45 0.7180526104412692 1102 46-47 0.5312095574964388 1102 48-49 0.6021425696465812 1102 50-51 0.5134141082433956 1102 52-53 0.4829140920856503 1102 54-55 0.5572802342630752 1102 56-57 0.6140829261231247 1102 58-59 0.6824258560510472 1102 60-61 0.5951826831055769 1102 62-63 0.5162033347856436 1102 64-65 0.4586485047919808 1102 66-67 0.5420854611483321 1102 68-69 0.47253331386546193 1102 70-71 0.4811527917248952 1102 72-73 0.5317611889050085 1102 74-75 0.525290552731903 1102 76-77 0.6024445103238385 1102 78-79 0.563318174101834 1102 80-81 0.5111310213702893 1102 82-83 0.5095883226227826 1102 84-85 0.49673828081117577 1102 86-87 0.5284645948258806 1102 88-89 0.5652815823215498 1102 90-91 0.5718761913087036 1102 92-93 0.6371541069694509 1102 94-95 0.6705376132733534 1102 96-97 0.6817079034920965 1102 98-99 0.5978470482586502 1102 100-101 0.7111206693587739 1102 102-103 0.7377129889576999 1102 104-105 0.7306030506433636 1102 106-107 0.4995688331942105 1102 108-109 0.6125856751913581 1102 110-111 0.5688463212201 1102 112-113 0.508283084213975 1102 114-115 0.5997928529700154 1102 116-117 0.5017810156755296 1102 118-119 0.570247326180862 1102 120-121 0.38584108006944007 1102 122-123 0.4263003593496748 1102 124-125 0.5957310758670218 1103 1 0.22626973971101094 1103 2 0.1891350764206301 1103 3 0.1994728292391592 1103 4 0.22328287498284993 1103 5 0.17999572181725654 1103 6 0.24828364738240793 1103 7 0.245529421979235 1103 8 0.310891430012056 1103 9 0.34804472662596453 1103 10-11 0.3849361004146843 1103 12-13 0.2552781517913232 1103 14-15 0.3829678803942116 1103 16-17 0.26273126467197017 1103 18-19 0.2980953795466803 1103 20-21 0.261140382280999 1103 22-23 0.44133499885379024 1103 24-25 0.48442206118998854 1103 26-27 0.4325304536106529 1103 28-29 0.44901907064190283 1103 30-31 0.49503241560574907 1103 32-33 0.48814849498025126 1103 34-35 0.49327042247553976 1103 36-37 0.5075659965913104 1103 38-39 0.5402652667051484 1103 40-41 0.48023174997619833 1103 42-43 0.6113649331796509 1103 44-45 0.622485667748812 1103 46-47 0.44588043687534906 1103 48-49 0.46869718133793725 1103 50-51 0.3610303392728085 1103 52-53 0.4147983395933892 1103 54-55 0.47251554971120413 1103 56-57 0.3867512215995106 1103 58-59 0.4059281734390865 1103 60-61 0.459703462318636 1103 62-63 0.4338117189945052 1103 64-65 0.5329562111227375 1103 66-67 0.5063072289418855 1103 68-69 0.5442719790737343 1103 70-71 0.554616469466275 1103 72-73 0.4047575250738973 1103 74-75 0.3574588511805672 1103 76-77 0.35999975015715435 1103 78-79 0.37720169637313106 1103 80-81 0.3166367747145955 1103 82-83 0.3537815763386334 1103 84-85 0.39910124381816914 1103 86-87 0.36850686065475813 1103 88-89 0.4884838975299175 1103 90-91 0.5032242394120914 1103 92-93 0.6632736164242772 1103 94-95 0.6619671414931787 1103 96-97 0.6585269887034144 1103 98-99 0.39112652386910796 1103 100-101 0.47400243402152853 1103 102-103 0.44515022925468983 1103 104-105 0.515699937080452 1103 106-107 0.5991521194664351 1103 108-109 0.46762596940749646 1103 110-111 0.37718604495653096 1103 112-113 0.4409343782458315 1103 114-115 0.4363851928086824 1103 116-117 0.3951504523094833 1103 118-119 0.3434055525616344 1103 120-121 0.32321037403392694 1103 122-123 0.46548958067162616 1103 124-125 0.4820083539714233 1104 1 -0.06225447447468824 1104 2 0.09508159437405439 1104 3 0.22845400671328875 1104 4 0.19466080619493198 1104 5 -0.01191256363491533 1104 6 -0.08299844104081444 1104 7 -0.1153882868067555 1104 8 0.01542605734374547 1104 9 0.06242034022904619 1104 10-11 0.13502552551889124 1104 12-13 0.08102061151481621 1104 14-15 0.16378936414680112 1104 16-17 0.3108689290371274 1104 18-19 0.14111036353264694 1104 20-21 0.28557486154322476 1104 22-23 0.2608906295170499 1104 24-25 0.38470804046026785 1104 26-27 0.23716034030532995 1104 28-29 0.19754944097650906 1104 30-31 0.12560986639679328 1104 32-33 0.27715725441566263 1104 34-35 0.33690514556955264 1104 36-37 0.36842375333893784 1104 38-39 0.4772183552410354 1104 40-41 0.23190517633578622 1104 42-43 0.26372076663369626 1104 44-45 0.3346143875917349 1104 46-47 0.21496268785309525 1104 48-49 0.2378749934506672 1104 50-51 0.22541671607740454 1104 52-53 0.2494800908366912 1104 54-55 0.23244897880685755 1104 56-57 0.21522391324485568 1104 58-59 0.29110080714419695 1104 60-61 0.390986053695201 1104 62-63 0.35208582454228576 1104 64-65 0.32106136458386203 1104 66-67 0.349663114703084 1104 68-69 0.41607259227444615 1104 70-71 0.4268050608750329 1104 72-73 0.48520370504267163 1104 74-75 0.3194719923454912 1104 76-77 0.25790515066895736 1104 78-79 0.22666484397585762 1104 80-81 0.10707804585875635 1104 82-83 0.1417775889245334 1104 84-85 0.2661815393133189 1104 86-87 0.21432852547055958 1104 88-89 0.20364653252259046 1104 90-91 0.16860042839379474 1104 92-93 0.16373804354029886 1104 94-95 0.16946687995744014 1104 96-97 0.17246060690952092 1104 98-99 0.20237487418214783 1104 100-101 0.2446459527635838 1104 102-103 0.2341971125534208 1104 104-105 0.14525961148858002 1104 106-107 0.17192443852394135 1104 108-109 0.295120316211932 1104 110-111 0.33243788375395056 1104 112-113 0.32743329991386716 1104 114-115 0.30596566936702985 1104 116-117 0.3614862762677653 1104 118-119 0.41559635696509645 1104 120-121 0.3959715275272373 1104 122-123 0.4654286227265736 1104 124-125 0.480891194008116 1105 1 0.1778323897140659 1105 2 0.29902182320529036 1105 3 0.3528519525958522 1105 4 0.3692494837574287 1105 5 0.3088088176475736 1105 6 0.3128120886888439 1105 7 0.264760649960607 1105 8 0.33501875769582057 1105 9 0.382779397586539 1105 10-11 0.47289687343405973 1105 12-13 0.37105272756449637 1105 14-15 0.377475404911074 1105 16-17 0.3954030572612055 1105 18-19 0.3491532919987179 1105 20-21 0.15468003254297003 1105 22-23 0.15551303302466124 1105 24-25 0.28299977768166684 1105 26-27 0.12742053137223763 1105 28-29 0.30234408380580646 1105 30-31 0.22791628524090868 1105 32-33 0.2983153929243585 1105 34-35 0.30272225131278674 1105 36-37 0.2751141357534195 1105 38-39 0.2990655913663076 1105 40-41 0.1608495371020453 1105 42-43 0.44519340386850814 1105 44-45 0.5073519777536717 1105 46-47 0.39198901217724824 1105 48-49 0.49636378109415347 1105 50-51 0.47930823875003625 1105 52-53 0.47779162236569306 1105 54-55 0.4516848709773882 1105 56-57 0.5144189850157801 1105 58-59 0.4740834522374868 1105 60-61 0.5572359211614355 1105 62-63 0.6280862205843576 1105 64-65 0.7235736200590637 1105 66-67 0.5964521918938672 1105 68-69 0.6001419167888287 1105 70-71 0.609752428598533 1105 72-73 0.5812289004909985 1105 74-75 0.6829409700891773 1105 76-77 0.6615997827396392 1105 78-79 0.6237306438716992 1105 80-81 0.6450530624644557 1105 82-83 0.6545568648535962 1105 84-85 0.6525065495262155 1105 86-87 0.6293441722509208 1105 88-89 0.5960197600783914 1105 90-91 0.6191221631516299 1105 92-93 0.6055493864035881 1105 94-95 0.5636529940050821 1105 96-97 0.511359773661944 1105 98-99 0.47974709214249955 1105 100-101 0.5663062772997947 1105 102-103 0.530187888203379 1105 104-105 0.5453278858677741 1105 106-107 0.5403829227118848 1105 108-109 0.5954970824192536 1105 110-111 0.5631392134574185 1105 112-113 0.5650704333800469 1105 114-115 0.6505286080927739 1105 116-117 0.6133628959083346 1105 118-119 0.5729532088048188 1105 120-121 0.5849986868141102 1105 122-123 0.6502537795062189 1105 124-125 0.7584747838332646 1106 1 -0.16228214464624102 1106 2 -0.3198735044839225 1106 3 -0.2067600597957444 1106 4 -0.028316755811410133 1106 5 -0.24254029920869158 1106 6 -0.04479159393109455 1106 7 -0.24632165200271317 1106 8 -0.21973895749752614 1106 9 -0.16001639569689985 1106 10-11 -0.09511955422150464 1106 12-13 -0.08644209664033298 1106 14-15 -0.21396035915147849 1106 16-17 0.015966214844837623 1106 18-19 -0.10705648760182385 1106 20-21 -0.1609289493940338 1106 22-23 -0.22753663566381732 1106 24-25 -4.017366279105062 1106 26-27 -1.1591865681818732 1106 28-29 -0.82967895113498 1106 30-31 -0.805048054818684 1106 32-33 -5.533110265833876 1106 34-35 -1.221403452307392 1106 36-37 -0.5503061228999329 1106 38-39 -0.22590743084338527 1106 40-41 -0.5799140745572089 1106 42-43 -0.3036655341159218 1106 44-45 -0.2507873958765998 1106 46-47 -0.37549047409287084 1106 48-49 -0.15171473336927477 1106 50-51 -0.20637473887052238 1106 52-53 -0.129607548143035 1106 54-55 -0.39663866017286864 1106 56-57 -0.2532572458837876 1106 58-59 -0.006125572591173523 1106 60-61 0.04520323938720594 1106 62-63 -0.0066406558118927705 1106 64-65 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-0.807858407912434 2216 104-105 -0.8235864786628575 2216 106-107 -0.9470057064393167 2216 108-109 -0.8754630992826513 2216 110-111 -0.8676069839921112 2216 112-113 -1.020282681476612 2216 114-115 -1.016785840345971 2216 116-117 -0.9284659482481246 2216 118-119 -1.0780752689033761 2216 120-121 -1.0163294560969973 2216 122-123 -1.1030501488600102 2216 124-125 -1.2050675122602712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27813.0 3 4856.0 4 3347.0 5 2934.0 6 3298.0 7 4025.0 8 4467.0 9 4879.0 10 5769.0 11 6675.0 12 6878.0 13 6939.0 14 7107.0 15 6832.0 16 6434.0 17 5944.0 18 5292.0 19 5081.0 20 5076.0 21 5321.0 22 6214.0 23 8274.0 24 11738.0 25 16391.0 26 20198.0 27 20932.0 28 24525.0 29 30427.0 30 36673.0 31 45292.0 32 58079.0 33 77126.0 34 107165.0 35 153110.0 36 251499.0 37 341034.0 38 107029.0 39 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.588704296863106 17.681130733049663 9.278909907341017 22.451255062746217 2 19.03652231688664 20.45720376227619 38.014769494833445 22.491504426003722 3 17.81828122945317 28.85498349332466 29.84711427325642 23.479621003965757 4 10.815444168294842 15.720387107758556 37.580708008822945 35.883460715123654 5 10.982133278887646 42.960068933775815 34.55118644302409 11.506611344312443 6 30.86869195812472 36.748492243549904 19.44960833629786 12.933207462027516 7 28.74732241420479 34.19094517480872 21.400546071778333 15.661186339208161 8 28.009368362259952 33.507605911425514 19.91649035237251 18.566535373942028 9 28.922527066767344 12.939793875224675 18.682108145257683 39.45557091275029 10-11 24.43193261607566 26.772098238231518 29.33865335967969 19.45731578601313 12-13 25.229124130532583 25.64169290929854 28.51406720420805 20.615115755960826 14-15 23.03363904213549 26.89105845011105 25.55028622566251 24.525016282090945 16-17 22.749384275874863 29.30770702847748 26.009045270300902 21.933863425346757 18-19 21.945014958326052 28.241458008544157 27.60111361233625 22.212413420793546 20-21 23.601133551512124 27.56191950732244 27.191809194031585 21.64513774713385 22-23 23.27302893641194 27.91636358598139 26.73992559802149 22.070681879585177 24-25 22.661391376748927 27.802480465958567 27.25225014785277 22.283878009439736 26-27 22.51853820524427 27.919741371792583 27.05072623254668 22.510994190416472 28-29 22.50111170132695 27.889359331283536 27.095197969349922 22.5143309980396 30-31 22.600478523539635 27.610199181789806 27.357199066207755 22.432123228462807 32-33 22.222706699735717 27.917843687482936 27.238121736049038 22.621327876732312 34-35 22.699772469698672 27.928436096860427 27.145039752917665 22.226751680523233 36-37 22.92854349714333 27.44612818498614 26.93836448387912 22.68696383399141 38-39 22.477894113217577 27.783133914333806 27.20155668630404 22.537415286144576 40-41 22.553361571363315 27.76954168569965 27.310362249629723 22.366734493307312 42-43 22.441643256142623 27.66310715964074 27.2948369499392 22.600412634277436 44-45 22.310558163645965 27.658400831082254 27.370795708658886 22.660245296612896 46-47 22.535267318749426 27.84945920272414 26.895420482642752 22.719852995883677 48-49 22.453104680223163 27.734248204159968 27.159454814872237 22.65319230074464 50-51 22.509151412006293 27.651763943748144 27.200578049484413 22.63850659476115 52-53 22.530380148083758 27.634895609118683 27.28257724980586 22.5521469929917 54-55 22.266117316637818 27.70384515028802 27.3821874660434 22.64785006703076 56-57 22.461806892909806 27.85661463053725 27.12322253724665 22.558355939306292 58-59 22.704719534739183 27.428822168407198 27.144920605149125 22.721537691704498 60-61 22.61177432209041 27.765060322265512 27.323531030072367 22.299634325571706 62-63 22.33132910571075 27.663289759406172 27.48953620154167 22.51584493334141 64-65 22.247718644994517 27.666529398940387 27.43097105957996 22.65478089648514 66-67 22.259253709598774 28.133948707371925 27.030974834007154 22.57582274902214 68-69 22.491892866449184 27.59827098571878 27.279764712314847 22.630071435517188 70-71 22.191446620537867 27.541348947221806 27.426182677650374 22.841021754589956 72-73 22.29845750722038 27.70510682975412 27.195579668468213 22.800855994557292 74-75 21.999241453198493 27.99556776120725 27.325368130514345 22.67982265507991 76-77 21.972899263843043 27.841910872857976 27.41896580899923 22.76622405429975 78-79 22.253297703805675 27.722167199933008 27.25752300035505 22.76701209590627 80-81 22.210125769274423 27.778212265757148 27.18186021145184 22.829801753516584 82-83 22.33713067611619 27.716888024528156 27.220268951531974 22.72571234782368 84-85 22.079552568278704 27.61451993728149 27.38705979125436 22.918867703185445 86-87 21.964690224511703 27.616725114872004 27.421205659475014 22.99737900114128 88-89 22.21181373945103 27.466824835616528 27.341726946085405 22.979634478847036 90-91 22.012660667735506 27.701690499862096 27.465786678856936 22.81986215354546 92-93 22.046358528004962 27.56575163561541 27.460240340127722 22.92764949625191 94-95 21.816968118551966 27.389447551801428 27.61088638862291 23.1826979410237 96-97 22.024849328796815 27.45396119230269 27.528881531046434 22.99230794785406 98-99 21.79909230431164 27.544913287896637 27.438709777886206 23.21728462990551 100-101 21.685587086705183 27.359948230547094 27.615196549164544 23.339268133583182 102-103 21.82190786748515 27.336819473383144 27.52822150450786 23.313051154623842 104-105 21.658175103495793 27.2339882155121 27.635997952750618 23.471838728241487 106-107 21.490289933360625 27.366784405341253 27.785092948033608 23.35783271326451 108-109 21.720372878610974 27.575513870826867 27.257837572593186 23.446275677968977 110-111 21.441632488085368 27.633919363925415 27.47002827951735 23.454419868471867 112-113 21.645022244246533 27.39828136549498 27.66058906419133 23.296107326067155 114-115 21.42274539170866 27.598463112733096 27.406438717433456 23.57235277812479 116-117 21.262888337125503 27.53826056112606 27.738867885352413 23.459983216396026 118-119 21.32416935752621 27.488155430922223 27.505388928550538 23.682286283001023 120-121 21.082843897894747 27.480236866911856 27.645581377321847 23.791337857871547 122-123 21.382012424698797 27.324890093462084 27.609311326187104 23.68378615565202 124-125 21.512815348657906 27.19284585661399 27.229873434892717 24.064465359835392 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 17.0 2 16.5 3 13.0 4 15.5 5 20.0 6 23.5 7 25.0 8 25.5 9 33.5 10 44.0 11 56.0 12 75.5 13 86.5 14 100.5 15 140.0 16 198.0 17 256.5 18 320.5 19 457.0 20 661.0 21 903.5 22 1308.0 23 2000.0 24 2791.5 25 3937.0 26 5918.5 27 8176.5 28 10125.5 29 12517.0 30 16708.0 31 20688.0 32 24118.5 33 28402.5 34 33318.0 35 36962.5 36 40887.5 37 46100.0 38 48069.0 39 47898.0 40 48551.0 41 50881.0 42 51764.0 43 52701.5 44 55252.5 45 56983.0 46 57403.0 47 56318.0 48 56409.5 49 55323.0 50 52578.5 51 49722.5 52 46975.5 53 45699.5 54 43957.0 55 40279.5 56 35769.5 57 31954.5 58 29332.0 59 26517.5 60 22653.5 61 18604.0 62 15160.5 63 11785.0 64 8808.0 65 6875.5 66 5320.5 67 4220.5 68 3228.5 69 2218.0 70 1660.0 71 1379.5 72 1164.0 73 1044.0 74 893.0 75 693.5 76 427.0 77 260.5 78 195.0 79 140.5 80 95.5 81 63.5 82 44.0 83 32.0 84 21.5 85 17.5 86 13.0 87 9.5 88 8.0 89 9.0 90 8.0 91 7.0 92 5.0 93 2.5 94 2.0 95 0.0 96 1.0 97 1.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.384207575768062E-4 2 0.0 3 0.0 4 6.92103787884031E-5 5 3.460518939420155E-4 6 5.536830303072248E-4 7 3.460518939420155E-4 8 4.8447265151882175E-4 9 0.001176576439402853 10-11 0.0010381556818260466 12-13 0.001384207575768062 14-15 0.0021455217424404963 16-17 0.0018340750378926826 18-19 0.00155723352273907 20-21 0.0021109165530462945 22-23 0.09412611515222823 24-25 0.0014188127651622636 26-27 0.0011073660606144497 28-29 8.651297348550389E-4 30-31 6.228934090956279E-4 32-33 0.001003550492431845 34-35 5.190778409130233E-4 36-37 3.460518939420155E-4 38-39 4.152622727304186E-4 40-41 6.92103787884031E-4 42-43 4.8447265151882175E-4 44-45 4.8447265151882175E-4 46-47 8.651297348550389E-4 48-49 2.076311363652093E-4 50-51 7.613141666724342E-4 52-53 8.305245454608372E-4 54-55 7.613141666724342E-4 56-57 5.882882197014265E-4 58-59 2.076311363652093E-4 60-61 3.806570833362171E-4 62-63 5.190778409130233E-4 64-65 3.460518939420155E-4 66-67 2.4223632575941088E-4 68-69 3.460518939420155E-5 70-71 1.7302594697100776E-4 72-73 6.92103787884031E-5 74-75 1.384207575768062E-4 76-77 1.0381556818260465E-4 78-79 6.92103787884031E-5 80-81 1.384207575768062E-4 82-83 0.0 84-85 1.0381556818260465E-4 86-87 6.92103787884031E-5 88-89 1.0381556818260465E-4 90-91 1.0381556818260465E-4 92-93 6.92103787884031E-5 94-95 1.0381556818260465E-4 96-97 1.384207575768062E-4 98-99 1.7302594697100776E-4 100-101 1.7302594697100776E-4 102-103 3.806570833362171E-4 104-105 3.1144670454781397E-4 106-107 2.4223632575941088E-4 108-109 2.076311363652093E-4 110-111 5.536830303072248E-4 112-113 1.384207575768062E-4 114-115 1.0381556818260465E-4 116-117 5.190778409130233E-4 118-119 6.574985984898295E-4 120-121 5.536830303072248E-4 122-123 4.152622727304186E-4 124-125 6.92103787884031E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1444870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.826928613285126 #Duplication Level Percentage of deduplicated Percentage of total 1 52.85859658920343 17.880439734214214 2 14.47469073404372 9.792686603197533 3 10.204373489683242 10.355478407364428 4 7.015812782121908 9.492935925800444 5 4.695346474206521 7.941457499881199 6 3.05638822296225 6.203293573957765 7 1.9630921961389287 4.648376570606207 8 1.2841829451233004 3.475197184886731 9 0.8361013806996118 2.545446754655541 >10 3.356837664304619 18.955512093937916 >50 0.17497386059587877 4.028933620931764 >100 0.0789897564945666 4.5308616662630845 >500 6.139044220954648E-4 0.1493803643031334 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.92103787884031E-5 0.0 4 0.0 0.0 0.0 2.768415151536124E-4 0.0 5 0.0 0.0 0.0 2.768415151536124E-4 0.0 6 0.0 0.0 0.0 2.768415151536124E-4 0.0 7 0.0 0.0 0.0 2.768415151536124E-4 0.0 8 0.0 0.0 0.0 2.768415151536124E-4 0.0 9 0.0 0.0 0.0 2.768415151536124E-4 0.0 10-11 0.0 0.0 0.0 5.536830303072248E-4 0.0 12-13 0.0 0.0 0.0 8.305245454608373E-4 0.0 14-15 0.0 0.0 0.0 8.305245454608373E-4 0.0 16-17 0.0 0.0 0.0 8.997349242492404E-4 0.0 18-19 0.0 0.0 0.0 9.34340113643442E-4 0.0 20-21 0.0 0.0 0.0 0.001003550492431845 0.0 22-23 0.0 0.0 0.0 0.0010727608712202481 0.0 24-25 0.0 0.0 0.0 0.0011765764394028528 0.0 26-27 0.0 0.0 0.0 0.0011765764394028528 0.0 28-29 0.0 0.0 0.0 0.0013842075757680623 0.0 30-31 0.0 0.0 0.0 0.002491573636382512 0.0 32-33 0.0 0.0 0.0 0.00577906662883166 0.0 34-35 0.0 0.0 0.0 0.01138510731069231 0.0 36-37 0.0 0.0 0.0 0.022285741969865804 0.0 38-39 0.0 0.0 0.0 0.04183767397758968 0.0 40-41 0.0 0.0 0.0 0.06523078200806992 0.0 42-43 0.0 0.0 0.0 0.09371085287949782 0.0 44-45 0.0 0.0 0.0 0.12983867060704424 0.0 46-47 0.0 0.0 0.0 0.17939330181954086 0.0 48-49 0.0 0.0 0.0 0.2293285901153737 0.0 50-51 0.0 0.0 0.0 0.2787448005702935 0.0 52-53 0.0 0.0 0.0 0.33518586447223625 0.0 54-55 0.0 0.0 0.0 0.40674939613944505 0.0 56-57 0.0 0.0 0.0 0.49706894045831107 0.0 58-59 0.0 0.0 0.0 0.5999155633378781 0.0 60-61 0.0 0.0 0.0 0.6985749583007468 0.0 62-63 0.0 0.0 0.0 0.8012831604227371 0.0 64-65 0.0 0.0 0.0 0.9225743492494134 0.0 66-67 0.0 0.0 0.0 1.0753562604248132 0.0 68-69 0.0 0.0 0.0 1.2646812516004902 0.0 70-71 0.0 0.0 0.0 1.4561863697080013 0.0 72-73 0.0 0.0 0.0 1.6399745305806057 0.0 74-75 0.0 0.0 0.0 1.8460138282336818 0.0 76-77 0.0 0.0 0.0 2.091537646985542 0.0 78-79 0.0 0.0 0.0 2.394056212669652 0.0 80-81 0.0 0.0 0.0 2.706229626194744 0.0 82-83 0.0 0.0 0.0 3.021586717144103 0.0 84-85 0.0 0.0 0.0 3.352966010782977 0.0 86-87 0.0 0.0 0.0 3.7136905050281337 0.0 88-89 0.0 0.0 0.0 4.148954577228402 0.0 90-91 0.0 0.0 0.0 4.618131735034986 0.0 92-93 0.0 0.0 0.0 5.07651207375058 0.0 94-95 0.0 0.0 0.0 5.565898662163378 0.0 96-97 0.0 0.0 0.0 6.089959650349167 0.0 98-99 0.0 0.0 0.0 6.688144954217334 0.0 100-101 0.0 0.0 0.0 7.321835182403953 0.0 102-103 0.0 0.0 0.0 7.961408292787587 0.0 104-105 0.0 0.0 0.0 8.633406465633588 0.0 106-107 0.0 0.0 0.0 9.348280468139002 0.0 108-109 0.0 0.0 0.0 10.085924685265802 0.0 110-111 0.0 0.0 0.0 10.862499740461079 0.0 112-113 0.0 0.0 0.0 11.64256991978517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 68.072556 1 GTACATG 13140 0.0 54.740196 1 TACATGG 13380 0.0 53.491516 2 ACATGGG 13540 0.0 51.497288 3 GTATCAA 2960 0.0 49.042603 2 CATGGGA 9920 0.0 48.279152 4 ATGGGAG 3860 0.0 44.235405 5 ATGGGAT 3935 0.0 41.577976 5 CATGGGG 3670 0.0 40.851677 4 TCAACGC 3660 0.0 39.825428 5 CAACGCA 3720 0.0 39.18308 6 ATCAACG 3720 0.0 39.18308 4 AACGCAG 3920 0.0 37.03217 7 GAGTACA 7570 0.0 33.24451 1 TATCAAC 4480 0.0 32.80155 3 AGTACAT 7445 0.0 32.76382 2 ATGGGGG 2020 0.0 31.514309 5 ATGGGAC 1945 0.0 31.2001 5 ATGGGCG 315 0.0 30.219332 5 ACGCAGA 4950 0.0 29.446678 8 >>END_MODULE