FastQCFastQC Report
Thu 26 May 2016
SRR936265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444870
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23370.16174465522849807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.11032134378871457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.10104715303106854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.076.820341
GTATCAA49450.050.5669822
GTACATG119050.047.1336671
TACATGG119950.046.3090022
ACATGGG123200.044.507483
CATGGGA88300.041.8037344
TCAACGC66600.038.886525
CAACGCA67700.038.402646
ATCAACG67650.038.282964
AACGCAG70000.037.2258347
TATCAAC72700.035.7874763
ATGGGAT35250.035.4686165
ATGGGAG32950.034.3306665
CATGGGG33800.032.762754
GAGTACA76950.031.9706271
AGTACAT74400.031.6888392
ATGGGAC18050.031.3350545
ACGCAGA83550.030.7613688
CGCAGAG83650.030.5823529
GGGACCG2751.8189894E-1228.1241257
Produced by FastQC (version 0.11.5)