##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1444870 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.571838296871 31.0 31.0 34.0 30.0 34.0 2 31.3320748579457 31.0 31.0 34.0 28.0 34.0 3 31.711272986497054 31.0 31.0 34.0 30.0 34.0 4 35.5494286683231 37.0 35.0 37.0 33.0 37.0 5 35.18570874888398 37.0 35.0 37.0 33.0 37.0 6 35.39866493179317 37.0 35.0 37.0 33.0 37.0 7 35.220970052669095 37.0 35.0 37.0 32.0 37.0 8 35.30922435928492 37.0 35.0 37.0 32.0 37.0 9 36.95923993162015 39.0 37.0 39.0 33.0 39.0 10-11 36.85977665810765 39.0 37.0 39.0 32.5 39.0 12-13 36.81110030660198 39.0 37.0 39.0 32.0 39.0 14-15 37.97543585236042 40.0 37.0 41.0 33.0 41.0 16-17 37.88970391799954 40.0 37.0 41.0 32.0 41.0 18-19 37.97794888121423 40.0 37.0 41.0 33.0 41.0 20-21 38.03158554056766 40.0 37.0 41.0 33.0 41.0 22-23 37.95610920013566 40.0 37.0 41.0 32.5 41.0 24-25 37.9410147625738 40.0 37.0 41.0 32.5 41.0 26-27 37.78345629710631 40.0 37.0 41.0 32.0 41.0 28-29 37.644027490362454 40.0 37.0 41.0 32.0 41.0 30-31 37.517949365686874 40.0 36.5 41.0 32.0 41.0 32-33 37.36355450663382 40.0 36.0 41.0 31.5 41.0 34-35 37.20598635171331 39.0 36.0 41.0 31.0 41.0 36-37 37.03999702395372 39.0 36.0 41.0 31.0 41.0 38-39 37.0520396298629 39.0 36.0 41.0 31.0 41.0 40-41 37.10528628873186 40.0 36.0 41.0 31.0 41.0 42-43 37.02654979340702 40.0 36.0 41.0 31.0 41.0 44-45 36.903065673728435 40.0 36.0 41.0 31.0 41.0 46-47 36.68421657311731 40.0 36.0 41.0 30.0 41.0 48-49 36.465892087177394 39.5 36.0 41.0 30.0 41.0 50-51 36.29685750275111 39.0 35.0 41.0 30.0 41.0 52-53 36.14693674863483 39.0 35.0 41.0 29.0 41.0 54-55 35.966031892142546 39.0 35.0 41.0 28.5 41.0 56-57 35.77837141057673 39.0 35.0 41.0 28.0 41.0 58-59 35.57537875379792 38.5 35.0 40.5 28.0 41.0 60-61 35.357746717697786 38.0 34.0 40.0 28.0 41.0 62-63 35.14147881816358 38.0 34.0 40.0 28.0 41.0 64-65 34.9070494231315 37.5 34.0 40.0 28.0 41.0 66-67 34.62250237045548 37.0 34.0 40.0 27.0 41.0 68-69 34.31483351443382 36.5 34.0 39.0 26.0 41.0 70-71 33.994344819949205 36.0 33.5 39.0 26.0 41.0 72-73 33.72556146919792 36.0 33.0 39.0 26.0 40.0 74-75 33.54065902122682 35.0 33.0 38.0 26.0 40.0 76-77 33.23268217901956 35.0 33.0 37.0 26.0 39.0 78-79 32.90001661049091 35.0 33.0 37.0 26.0 39.0 80-81 32.62427657851572 35.0 33.0 36.0 26.0 39.0 82-83 32.36719324229861 35.0 33.0 36.0 26.0 37.0 84-85 32.11580799656717 35.0 33.0 35.5 26.0 37.0 86-87 31.931174776969556 35.0 32.0 35.0 26.0 36.5 88-89 31.773728086263816 35.0 32.0 35.0 25.0 36.0 90-91 31.648129589513246 35.0 32.0 35.0 25.0 36.0 92-93 31.527638126613468 35.0 32.0 35.0 25.0 36.0 94-95 31.41425110909632 35.0 32.0 35.0 25.0 35.5 96-97 31.36129374961069 35.0 32.0 35.0 25.0 35.0 98-99 31.290933786430614 35.0 32.0 35.0 25.0 35.0 100-101 31.217311938098234 35.0 32.0 35.0 24.0 35.0 102-103 30.174365167800566 33.5 30.0 34.5 21.5 35.0 104-105 30.95872742876522 34.0 31.5 35.0 24.0 35.0 106-107 31.117128184542555 34.5 32.0 35.0 24.0 35.0 108-109 31.094960446268523 35.0 32.0 35.0 24.0 35.0 110-111 31.063852803366395 35.0 32.0 35.0 24.0 35.0 112-113 30.990099455314322 35.0 32.0 35.0 23.0 35.0 114-115 30.969489988718706 35.0 32.0 35.0 23.5 35.0 116-117 30.871895741485396 35.0 32.0 35.0 23.0 35.0 118-119 30.746615612477246 35.0 32.0 35.0 21.5 35.0 120-121 30.55236526469509 34.0 32.0 35.0 19.0 35.0 122-123 30.3162917771149 34.0 32.0 35.0 10.0 35.0 124-125 28.14833722064961 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23579921228437328 1101 2 0.5543227949799459 1101 3 0.355761371187171 1101 4 0.1897684820306651 1101 5 0.5047566153750722 1101 6 0.3229208589141521 1101 7 0.4965600959089471 1101 8 0.44000287602219856 1101 9 0.39898206102127176 1101 10-11 0.38753421021018397 1101 12-13 0.45620659767640603 1101 14-15 0.4411778482898896 1101 16-17 0.4728314450559523 1101 18-19 0.4795488069928595 1101 20-21 0.4250369532948781 1101 22-23 0.44091560430001664 1101 24-25 0.24649807853027994 1101 26-27 0.373839060871191 1101 28-29 0.4140227593869241 1101 30-31 0.33933074257026163 1101 32-33 0.21521851048915153 1101 34-35 0.2052171901811164 1101 36-37 0.20394437217674266 1101 38-39 0.2581541975764807 1101 40-41 0.15922941444009098 1101 42-43 0.2097177639651946 1101 44-45 0.24844124241022314 1101 46-47 0.17686189078172276 1101 48-49 0.1675489333325686 1101 50-51 0.07457312172823549 1101 52-53 0.012594574737619268 1101 54-55 0.044025371846466044 1101 56-57 -0.12198237849253957 1101 58-59 -0.07038557803247869 1101 60-61 -0.02626175383191054 1101 62-63 -0.08396777129849653 1101 64-65 -0.1433183527770936 1101 66-67 -0.11726668730668877 1101 68-69 -0.10801596618805576 1101 70-71 -0.10105212717031264 1101 72-73 -0.06692893851674597 1101 74-75 -0.17788935404568917 1101 76-77 -0.11294650955171193 1101 78-79 -0.09634131066479057 1101 80-81 -0.025965948564937946 1101 82-83 -0.14167822210462333 1101 84-85 -0.2353930703636422 1101 86-87 -0.08143328023755458 1101 88-89 -0.12646086336909335 1101 90-91 -0.10904759490657412 1101 92-93 -0.16192418108497364 1101 94-95 -0.1376024048929949 1101 96-97 -0.1997940095981683 1101 98-99 -0.23193386851495745 1101 100-101 -0.104053689644104 1101 102-103 -0.09018502898551972 1101 104-105 -0.23068183448727453 1101 106-107 -0.21427950725735911 1101 108-109 -0.34149057635679014 1101 110-111 -0.2386330335109932 1101 112-113 -0.25031455472166186 1101 114-115 -0.28826112432723505 1101 116-117 -0.2799958844366017 1101 118-119 -0.18297564652570486 1101 120-121 -0.16094922552209923 1101 122-123 -0.057050485752650815 1101 124-125 -0.09811247186988936 1102 1 0.09015279761805317 1102 2 0.5345816348522483 1102 3 0.25360899211611354 1102 4 0.2547775465239823 1102 5 0.449606979547859 1102 6 0.25975496116291197 1102 7 0.46472285533824476 1102 8 0.3677183210266932 1102 9 0.37090483281269826 1102 10-11 0.3591431498078421 1102 12-13 0.35319137920459553 1102 14-15 0.3378129538458339 1102 16-17 0.33753711187767266 1102 18-19 0.3945028711994141 1102 20-21 0.4019372311239877 1102 22-23 0.4150162838980549 1102 24-25 0.20658822165050594 1102 26-27 0.20914207620913317 1102 28-29 0.27935808191720213 1102 30-31 0.24006060155286946 1102 32-33 0.17791887721083555 1102 34-35 0.2976224510919607 1102 36-37 0.2951221828068924 1102 38-39 0.2061064425121515 1102 40-41 0.04116956648142889 1102 42-43 0.08946248457488792 1102 44-45 0.22878829780399457 1102 46-47 0.1741063007453434 1102 48-49 0.12671470523231676 1102 50-51 0.02046355287313162 1102 52-53 0.14600929999903656 1102 54-55 0.1718279570590724 1102 56-57 0.08726264798406191 1102 58-59 0.09010812620697095 1102 60-61 0.18878239773788863 1102 62-63 0.11770147346513227 1102 64-65 0.13364811656293085 1102 66-67 0.13306865115416144 1102 68-69 0.12467626797855047 1102 70-71 0.11616316171461705 1102 72-73 0.18483497364847068 1102 74-75 0.2057189276195004 1102 76-77 0.1627721980331529 1102 78-79 0.0795249936345499 1102 80-81 0.21939785782130627 1102 82-83 0.17394855017108313 1102 84-85 0.22206076284967935 1102 86-87 0.20429062071254833 1102 88-89 0.19373997330434634 1102 90-91 0.2800300490898344 1102 92-93 0.22920751055020006 1102 94-95 0.10455617116910076 1102 96-97 0.186128983628155 1102 98-99 0.22318693298378633 1102 100-101 0.2526221742706056 1102 102-103 0.2951493297453851 1102 104-105 0.3064997134541798 1102 106-107 0.1174494851023411 1102 108-109 0.14793013242605113 1102 110-111 0.15538189183836337 1102 112-113 0.1173223366509788 1102 114-115 0.21661581999861568 1102 116-117 0.210531600572736 1102 118-119 0.17667087009624893 1102 120-121 0.09055462011706794 1102 122-123 0.12556440550529047 1102 124-125 -0.045982873511370315 1103 1 -0.05772053567564939 1103 2 0.5950869621696846 1103 3 0.25425774187585404 1103 4 0.2098329002947139 1103 5 0.27584746081952005 1103 6 0.17796869983604324 1103 7 0.31622355976872996 1103 8 0.23441788093102645 1103 9 0.24148262432901646 1103 10-11 0.37733974833002293 1103 12-13 0.3552631577974381 1103 14-15 0.3188870567620441 1103 16-17 0.4215096836731078 1103 18-19 0.3972896814074218 1103 20-21 0.2909304800841852 1103 22-23 0.3301669884276137 1103 24-25 0.21056860063608696 1103 26-27 0.1937924270373088 1103 28-29 0.22923790270850475 1103 30-31 0.16688473964106265 1103 32-33 0.27374004896307014 1103 34-35 0.1354338222445861 1103 36-37 0.11679704306017413 1103 38-39 0.15395282712920988 1103 40-41 0.13129981181346295 1103 42-43 0.17962958618527125 1103 44-45 0.3467023315255062 1103 46-47 0.2642162354938691 1103 48-49 0.27597450830882764 1103 50-51 0.21927672126471975 1103 52-53 0.10177413712797545 1103 54-55 0.23755558123600196 1103 56-57 0.17655332314748762 1103 58-59 0.15712254969352557 1103 60-61 0.20154804530864823 1103 62-63 0.14637912568056777 1103 64-65 0.09608542621313632 1103 66-67 -0.026914941857413055 1103 68-69 6.548811158069157E-5 1103 70-71 0.023170709325597727 1103 72-73 -0.024320542012475244 1103 74-75 -0.05393801176380464 1103 76-77 0.007073246455568949 1103 78-79 -0.12244780801724175 1103 80-81 -0.047490540971544704 1103 82-83 -0.0032779356223997524 1103 84-85 0.016636648143432353 1103 86-87 -0.013637382727374359 1103 88-89 -0.01091739210583853 1103 90-91 0.035417604745784814 1103 92-93 -0.023729300747632465 1103 94-95 -0.044369943881935825 1103 96-97 0.11372165438071491 1103 98-99 0.05900955124587526 1103 100-101 0.11817443389733029 1103 102-103 0.19053526055465753 1103 104-105 0.11551523470301106 1103 106-107 0.0011565331720433392 1103 108-109 -0.02509721453617786 1103 110-111 0.047727575987984494 1103 112-113 0.05362497821574408 1103 114-115 0.07210383873587034 1103 116-117 0.16240076871068254 1103 118-119 0.16851007764663706 1103 120-121 0.0625914050606795 1103 122-123 0.09778289586420286 1103 124-125 0.060175394481326805 1104 1 0.12923446408293415 1104 2 0.36277603239001976 1104 3 0.23885489636702673 1104 4 0.13536200721052438 1104 5 0.33838970890025166 1104 6 0.16015107474149204 1104 7 0.29152412468592104 1104 8 0.24216114940349343 1104 9 0.2784784638989706 1104 10-11 0.237354354095082 1104 12-13 0.2394799789713673 1104 14-15 0.16015266843377418 1104 16-17 0.46473791987609303 1104 18-19 0.3250973681439362 1104 20-21 0.315999183510705 1104 22-23 0.28623934530720874 1104 24-25 0.19299088428567757 1104 26-27 0.11439661482802421 1104 28-29 0.1453626874444751 1104 30-31 0.05718146199472329 1104 32-33 0.17115376228770884 1104 34-35 0.05099057147607766 1104 36-37 -0.02379843357864786 1104 38-39 0.04322614002251868 1104 40-41 -0.03389108915703787 1104 42-43 -0.07130741589092793 1104 44-45 0.01013188989223579 1104 46-47 0.04736548790402395 1104 48-49 0.059410444123933814 1104 50-51 -0.06211752575377716 1104 52-53 -0.10476154037029062 1104 54-55 -0.10772822527583514 1104 56-57 -0.1433402921616036 1104 58-59 -0.1344593190396779 1104 60-61 -0.14496678980313504 1104 62-63 -0.12263683604669495 1104 64-65 -0.23594425205767067 1104 66-67 -0.24743755061604844 1104 68-69 -0.13139726115209527 1104 70-71 -0.11139385378901068 1104 72-73 -0.23801706801314992 1104 74-75 -0.1527094979305872 1104 76-77 -0.07202924336466054 1104 78-79 -0.03563987181586725 1104 80-81 -0.21346594856493795 1104 82-83 -0.2988275026801688 1104 84-85 -0.20886429338522206 1104 86-87 -0.20868148167640044 1104 88-89 -0.16130798567125026 1104 90-91 -0.14569327835981127 1104 92-93 -0.13089900122885822 1104 94-95 -0.09780923942536646 1104 96-97 -0.14673645564133508 1104 98-99 -0.09973962391064362 1104 100-101 -0.14699433712611665 1104 102-103 -0.10412387790638533 1104 104-105 -0.15739046758079667 1104 106-107 -0.09220216912785872 1104 108-109 -0.06855892168053046 1104 110-111 -0.0954226018563169 1104 112-113 -0.061240813714469766 1104 114-115 -0.0234229948308311 1104 116-117 -0.0765336542207713 1104 118-119 -0.05392888393577522 1104 120-121 -0.025832319047282226 1104 122-123 -0.07076451452962829 1104 124-125 -0.011107076186434028 1105 1 0.1829016733584261 1105 2 0.5969581275461451 1105 3 0.2710220105300536 1105 4 0.22637840085763372 1105 5 0.45645332042975184 1105 6 0.18242551786579497 1105 7 0.22531727222251163 1105 8 0.20419704734313626 1105 9 0.3243107837388095 1105 10-11 0.1434894590150506 1105 12-13 0.22215557451574597 1105 14-15 0.2747849223185739 1105 16-17 0.25838752554916056 1105 18-19 0.28693427053359954 1105 20-21 0.27248064983309916 1105 22-23 0.23799871154633934 1105 24-25 0.08573842227609418 1105 26-27 0.1855563320477387 1105 28-29 0.1288032756760984 1105 30-31 0.07260907439115272 1105 32-33 0.1447428039282599 1105 34-35 0.12699030484932905 1105 36-37 0.08356902096227259 1105 38-39 0.15895511081208014 1105 40-41 0.01062709993279043 1105 42-43 0.01010758864640593 1105 44-45 0.06399955648717537 1105 46-47 0.2510104393031156 1105 48-49 0.266437150457179 1105 50-51 0.23057033306189822 1105 52-53 0.18533976610078184 1105 54-55 0.1907653920539687 1105 56-57 0.11289035401066627 1105 58-59 0.21443513496799937 1105 60-61 0.18817875487945201 1105 62-63 0.14149233525865412 1105 64-65 0.2017617599857502 1105 66-67 0.1875644802690033 1105 68-69 0.3125873431020878 1105 70-71 0.23003339452937155 1105 72-73 0.1883246609443816 1105 74-75 0.2871220243678323 1105 76-77 0.18668563128958482 1105 78-79 0.18907911762197216 1105 80-81 0.1820405331157744 1105 82-83 0.1905889940314367 1105 84-85 0.10137931926691834 1105 86-87 0.14536570969923446 1105 88-89 0.18019049954577326 1105 90-91 0.26548512612076536 1105 92-93 0.18074206303286644 1105 94-95 0.28248616664249937 1105 96-97 0.08421210800449686 1105 98-99 0.07458223199864733 1105 100-101 0.06056104249736194 1105 102-103 0.13023177801101227 1105 104-105 0.149240430561548 1105 106-107 0.3114115783656928 1105 108-109 0.3094157791829666 1105 110-111 0.28648123788073576 1105 112-113 0.27456242276007004 1105 114-115 0.27697521066199116 1105 116-117 0.3631964212766974 1105 118-119 0.42461623835390583 1105 120-121 0.43143382512450756 1105 122-123 0.2886312913224387 1105 124-125 0.2929775169972757 1106 1 0.2023811914562792 1106 2 0.5095002613984221 1106 3 0.30418480027606876 1106 4 0.1860738852605337 1106 5 0.3306797929169534 1106 6 0.25982657909325724 1106 7 0.3419058472798753 1106 8 0.23923691536415248 1106 9 0.1597608505770367 1106 10-11 0.1649811337901994 1106 12-13 0.24091536912079192 1106 14-15 0.2296205207253692 1106 16-17 0.18307601164044485 1106 18-19 0.19540444416143998 1106 20-21 0.2248982880033381 1106 22-23 0.13417760309258853 1106 24-25 0.12394751758044009 1106 26-27 0.17209992770219884 1106 28-29 0.15406464196477287 1106 30-31 0.2081253921652717 1106 32-33 0.134795785731896 1106 34-35 0.23083253706444395 1106 36-37 0.23298704712769336 1106 38-39 0.094197425930858 1106 40-41 0.14091050313557218 1106 42-43 0.24625968427510259 1106 44-45 0.1792170762885732 1106 46-47 0.10967997198110169 1106 48-49 0.06172782099167051 1106 50-51 0.07099329328330128 1106 52-53 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-0.9954357145306858 2216 56-57 -1.4560010842987694 2216 58-59 -1.537512155143375 2216 60-61 -1.106923851562719 2216 62-63 -1.037438281827967 2216 64-65 -0.9247477274786178 2216 66-67 -0.9085685066649134 2216 68-69 -0.810005937380275 2216 70-71 -0.8212069882921114 2216 72-73 -0.9088598691469727 2216 74-75 -0.8837110885210677 2216 76-77 -0.6445174198470269 2216 78-79 -0.5440201269265543 2216 80-81 -0.5583945453021144 2216 82-83 -0.6847370084802407 2216 84-85 -0.6461272453362952 2216 86-87 -0.5590215522323767 2216 88-89 -0.564708787513716 2216 90-91 -0.5993279509215483 2216 92-93 -0.7136439829736538 2216 94-95 -0.7470602561708581 2216 96-97 -0.6700866512878143 2216 98-99 -0.6803396192890716 2216 100-101 -0.8827975468067706 2216 102-103 -0.9229670795763134 2216 104-105 -0.7924766598689388 2216 106-107 -0.7425275084281537 2216 108-109 -0.6174708306972114 2216 110-111 -0.7689125037249731 2216 112-113 -0.6883196538543501 2216 114-115 -0.7966457121601778 2216 116-117 -0.7288581115713804 2216 118-119 -0.8167760794127972 2216 120-121 -0.8827098897958194 2216 122-123 -0.8284797449149117 2216 124-125 -0.9873534559562813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55.0 3 40.0 4 93.0 5 214.0 6 524.0 7 1123.0 8 1814.0 9 3244.0 10 5152.0 11 6455.0 12 6972.0 13 8591.0 14 11736.0 15 8425.0 16 4421.0 17 3862.0 18 3816.0 19 3540.0 20 3680.0 21 3834.0 22 4294.0 23 5572.0 24 7471.0 25 10102.0 26 13692.0 27 17836.0 28 22552.0 29 29049.0 30 36952.0 31 47277.0 32 59315.0 33 75942.0 34 102558.0 35 143723.0 36 239867.0 37 392620.0 38 157997.0 39 460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.13745723506517 18.68235522251617 10.339972575037743 22.840214967380916 2 18.811472091125594 21.201179627411044 37.12538835861947 22.861959922843898 3 17.94176496277517 27.679741208372523 31.302837835125537 23.07565599372677 4 11.561324936724366 16.084157110437005 37.50367680391786 34.85084114892077 5 10.711295332481482 42.27762714564223 35.08357486417137 11.92750265770492 6 30.96416978690124 37.303356011267454 18.444565947109428 13.287908254721877 7 27.338722514828323 34.24294227162305 22.184833237592308 16.23350197595631 8 27.552859426799643 33.49761570245074 20.120426059091823 18.829098811657797 9 27.728653788922188 11.622914172209263 19.52722390249642 41.121208136372125 10-11 24.37866382442711 26.003308256106084 29.832683909279034 19.785344010187767 12-13 25.481981077882438 25.195623135645423 29.344681528442006 19.977714258030137 14-15 22.921793283061202 26.43660898094949 26.726600568425035 23.914997167564266 16-17 22.083359143063884 28.735867658544706 27.20410196498851 21.9766712334029 18-19 21.244852478077615 27.116072726266033 29.768179836248244 21.870894959408112 20-21 22.532753811761612 26.51491137610996 29.75381868264965 21.198516129478776 22-23 22.343428820585935 26.99983389509091 29.141133804425312 21.515603479897845 24-25 21.82761142096224 27.04282289853859 29.512319278661497 21.617246401837676 26-27 21.799435243309087 27.06516849266716 29.340079038252576 21.79531722577118 28-29 21.68423456781579 26.895983721718906 29.55566936817845 21.864112342286848 30-31 21.791683680884784 26.671015385467207 29.747935800452634 21.789365133195375 32-33 21.646411095807927 26.969242907666434 29.564182244769427 21.820163751756212 34-35 22.12425088260719 27.000443639449166 29.266984435245597 21.608321042698048 36-37 22.18341442482715 26.63243059929267 29.081163011205156 22.102991964675024 38-39 21.93875573581014 26.92833957380249 29.17812675188771 21.954777938499657 40-41 22.027664090078723 26.89647750194741 29.239699502273385 21.836158905700483 42-43 21.979002359448767 27.0174532764777 28.982045626160602 22.02149873791293 44-45 21.913796015327367 27.10633638256401 28.782455756622667 22.197411845485956 46-47 22.258203844484637 27.180164447203918 28.085842298744602 22.475789409566843 48-49 22.33036488024209 27.216857195856992 28.006752734732977 22.446025189167937 50-51 22.339834989894516 27.272384340651733 27.886707826905504 22.501072842548243 52-53 22.47061563629178 27.302653322475805 27.877103227599342 22.34962781363307 54-55 22.185142143633836 27.333086004633262 27.94833921777223 22.533432633960675 56-57 22.370543917995285 27.45835914437688 27.789928675002333 22.381168262625494 58-59 22.567628689950546 26.848613269685245 27.92058108296573 22.663176957398484 60-61 22.50143095726369 27.196528051261147 28.005754282316968 22.296286709158196 62-63 22.077847923200626 27.137592651998766 28.32542769847919 22.45913172632142 64-65 22.187093599400352 27.058571518546227 28.33382588768792 22.420508994365502 66-67 22.26033879958044 27.463072811352703 27.874538053267617 22.40205033579924 68-69 22.484647269591385 27.115866829727786 27.81936205451474 22.580123846166092 70-71 22.169005915546183 26.91192847359252 28.09507231611594 22.82399329474536 72-73 22.265456701096216 27.139890416514834 27.83204581562802 22.762607066760935 74-75 22.11427258400635 27.462849367517013 27.822537946556796 22.600340101919837 76-77 21.85727450567164 27.25850763168449 28.099382329623296 22.78483553302057 78-79 22.131195194031296 27.2600995245247 27.784783406119583 22.823921875324423 80-81 22.016820274025886 27.32763337159531 27.66728483348081 22.988261520897996 82-83 22.29637347871209 27.183769883716142 27.650074972168763 22.869781665403 84-85 21.961064276783667 27.1684585829091 27.799242630038652 23.07123451026858 86-87 21.884901437226706 27.08472619606247 27.773440582033217 23.256931784677608 88-89 22.09539293029649 26.87047251437947 27.796239831595614 23.237894723728427 90-91 21.97602092407272 27.197384075640052 27.802605542609125 23.023989457678102 92-93 22.12171704325829 26.988945353120613 27.69669191119601 23.192645692425085 94-95 21.77263462423565 26.871900640548706 27.921521536763205 23.43394319845244 96-97 22.0156998670453 26.814077279834002 27.92363652219291 23.246586330927784 98-99 21.80678588063976 26.873737328295682 27.62741261567786 23.6920641753867 100-101 21.796807032915925 26.691734787312015 27.763947170077717 23.747511009694342 102-103 21.884458089740118 26.530579758191276 27.69761243469095 23.887349717377656 104-105 21.849176860529134 26.33167331607912 27.824754501200633 23.99439532219111 106-107 21.672840249545633 26.378432325212163 27.99463756500603 23.954089860236174 108-109 21.827843647243576 26.632423464956144 27.404643076392816 24.135089811407465 110-111 21.69851280120482 26.600635077072997 27.545049222625796 24.155802899096386 112-113 21.708899903128376 26.351746786779636 27.742697336566774 24.196655973525218 114-115 21.46171256064481 26.56458159623614 27.56137685741768 24.412328985701375 116-117 21.313305279567384 26.51440841696046 27.66150240169126 24.5107839017809 118-119 21.487849000132552 26.46495004383213 27.357270440255927 24.689930515779395 120-121 21.294151928592786 26.49773253302916 27.496290102880433 24.711825435497627 122-123 21.3772787898042 26.227530487108513 27.407285898098756 24.987904824988526 124-125 21.357172421335026 26.190581659166195 27.195912508184534 25.256333411314248 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 4.5 2 8.5 3 15.0 4 20.5 5 24.5 6 39.5 7 77.0 8 105.5 9 154.5 10 237.5 11 306.5 12 418.5 13 557.5 14 673.0 15 814.5 16 961.0 17 1084.5 18 1245.5 19 1474.5 20 1723.5 21 1937.0 22 2368.0 23 3155.5 24 3944.0 25 5050.5 26 6777.5 27 8900.0 28 10827.5 29 13246.5 30 17487.5 31 21378.0 32 24807.0 33 28967.5 34 33696.5 35 37117.0 36 40721.0 37 45432.0 38 47188.5 39 47518.5 40 48442.5 41 50571.0 42 51777.5 43 52526.0 44 54494.0 45 56213.5 46 56595.5 47 55840.0 48 56238.5 49 55438.5 50 52916.0 51 49081.5 52 46169.0 53 44729.5 54 42935.5 55 39743.5 56 35191.5 57 31405.5 58 28491.0 59 25879.5 60 21954.5 61 17648.0 62 14420.5 63 11054.5 64 8167.5 65 6547.5 66 4937.0 67 3689.5 68 2799.0 69 1854.5 70 1351.0 71 1215.0 72 1066.5 73 964.5 74 739.5 75 553.0 76 351.0 77 183.5 78 101.0 79 62.5 80 37.5 81 13.5 82 3.5 83 1.5 84 1.0 85 3.0 86 2.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06436565227321489 2 4.8447265151882175E-4 3 6.92103787884031E-5 4 6.92103787884031E-5 5 0.06609591174292498 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.460518939420155E-5 16-17 1.0381556818260465E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.460518939420155E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.076311363652093E-4 36-37 0.0 38-39 0.0 40-41 3.460518939420155E-5 42-43 0.0028376255303245272 44-45 0.009274190757646017 46-47 0.01045076719704887 48-49 0.008443666212185178 50-51 0.007613141666724343 52-53 0.0066788015530809 54-55 0.0068518275000519074 56-57 0.005190778409130233 58-59 0.0034951241288143572 60-61 0.002283942500017303 62-63 0.001003550492431845 64-65 0.0014534179545564654 66-67 0.0023185476894115042 68-69 8.997349242492404E-4 70-71 0.002283942500017303 72-73 0.0024569684469883104 74-75 6.228934090956279E-4 76-77 3.806570833362171E-4 78-79 0.0 80-81 4.498674621246202E-4 82-83 3.460518939420155E-5 84-85 1.0381556818260465E-4 86-87 2.768415151536124E-4 88-89 0.001003550492431845 90-91 0.001868680227286884 92-93 1.7302594697100776E-4 94-95 0.0010381556818260466 96-97 0.001176576439402853 98-99 0.001176576439402853 100-101 4.8447265151882175E-4 102-103 6.228934090956279E-4 104-105 3.806570833362171E-4 106-107 2.768415151536124E-4 108-109 6.228934090956279E-4 110-111 4.152622727304186E-4 112-113 0.012077211098576342 114-115 0.020105615038031103 116-117 0.018202329621350016 118-119 0.010623793144019877 120-121 0.005952092575802668 122-123 0.005467619924283846 124-125 0.0049831472727650235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1444870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.17282907820572 #Duplication Level Percentage of deduplicated Percentage of total 1 39.987704166282015 11.265667547065176 2 17.69844997200855 9.972308120211434 3 12.896739076751192 10.90012877026584 4 9.217695494901443 10.387542386912207 5 6.140334464504535 8.649529667575104 6 4.038212494520176 6.8260722233754985 7 2.549864121150209 5.028582024046984 8 1.7583439677102546 3.963001925039606 9 1.131759143682382 2.8696371221394195 >10 4.233071372538295 19.85851453819578 >50 0.24111019534530015 4.695436110191234 >100 0.10522648315266271 5.0638805652254195 >500 7.445237265050899E-4 0.1427721455001207 >1k 7.445237265050899E-4 0.3769268542561953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2337 0.16174465522849807 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 0.11032134378871457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.10104715303106854 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.460518939420155E-5 0.0 12-13 0.0 0.0 0.0 6.92103787884031E-5 0.0 14-15 0.0 0.0 0.0 6.92103787884031E-5 0.0 16-17 0.0 0.0 0.0 6.92103787884031E-5 0.0 18-19 0.0 0.0 0.0 6.92103787884031E-5 0.0 20-21 0.0 0.0 0.0 1.7302594697100776E-4 0.0 22-23 0.0 0.0 0.0 4.1526227273041866E-4 0.0 24-25 0.0 0.0 0.0 5.536830303072248E-4 0.0 26-27 0.0 0.0 0.0 6.228934090956279E-4 0.0 28-29 0.0 0.0 0.0 7.613141666724342E-4 0.0 30-31 0.0 0.0 0.0 0.0019724957954694883 0.0 32-33 0.0 0.0 0.0 0.005536830303072249 0.0 34-35 0.0 0.0 0.0 0.011177476174327104 0.0 36-37 0.0 0.0 0.0 0.02145521742440496 0.0 38-39 0.0 0.0 0.0 0.04086872867455203 0.0 40-41 0.0 0.0 0.0 0.06440025746260909 0.0 42-43 0.0 0.0 0.0 0.09339940617494999 0.0 44-45 0.0 0.0 0.0 0.12980406541765005 0.0 46-47 0.0 0.0 0.0 0.17897803954681044 0.0 48-49 0.0 0.0 0.0 0.22884411746385488 0.0 50-51 0.0 0.0 0.0 0.27843335386574575 0.0 52-53 0.0 0.0 0.0 0.33546270598738986 0.0 54-55 0.0 0.0 0.0 0.4074068947379349 0.0 56-57 0.0 0.0 0.0 0.4981417013295314 0.0 58-59 0.0 0.0 0.0 0.6018188487545593 0.0 60-61 0.0 0.0 0.0 0.7021392928083496 0.0 62-63 0.0 0.0 0.0 0.8054703883394354 0.0 64-65 0.0 0.0 0.0 0.9272460498176307 0.0 66-67 0.0 0.0 0.0 1.080962301106674 0.0 68-69 0.0 0.0 0.0 1.2707371597444754 0.0 70-71 0.0 0.0 0.0 1.4640763528898795 0.0 72-73 0.0 0.0 0.0 1.6487988538761273 0.0 74-75 0.0 0.0 0.0 1.8566722265670959 0.0 76-77 0.0 0.0 0.0 2.1047568293341268 0.0 78-79 0.0 0.0 0.0 2.4087288129727935 0.0 80-81 0.0 0.0 0.0 2.722148013316077 0.0 82-83 0.0 0.0 0.0 3.0375051042654357 0.0 84-85 0.0 0.0 0.0 3.37061465737402 0.0 86-87 0.0 0.0 0.0 3.733623094119194 0.0 88-89 0.0 0.0 0.0 4.170652030978566 0.0 90-91 0.0 0.0 0.0 4.642978261020023 0.0 92-93 0.0 0.0 0.0 5.106583983334141 0.0 94-95 0.0 0.0 0.0 5.600330825610609 0.0 96-97 0.0 0.0 0.0 6.128682857281278 0.0 98-99 0.0 0.0 0.0 6.734239066490411 0.0 100-101 0.0 0.0 0.0 7.375196384449811 0.0 102-103 0.0 0.0 0.0 8.021448296386527 0.0 104-105 0.0 0.0 0.0 8.700921190141674 0.0 106-107 0.0 0.0 0.0 9.4246887263214 0.0 108-109 0.0 0.0 0.0 10.168042799698242 0.0 110-111 0.0 0.0 0.0 10.953580598946619 0.0 112-113 0.0 0.0 0.0 11.743686283195029 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3280 0.0 76.82034 1 GTATCAA 4945 0.0 50.566982 2 GTACATG 11905 0.0 47.133667 1 TACATGG 11995 0.0 46.309002 2 ACATGGG 12320 0.0 44.50748 3 CATGGGA 8830 0.0 41.803734 4 TCAACGC 6660 0.0 38.88652 5 CAACGCA 6770 0.0 38.40264 6 ATCAACG 6765 0.0 38.28296 4 AACGCAG 7000 0.0 37.225834 7 TATCAAC 7270 0.0 35.787476 3 ATGGGAT 3525 0.0 35.468616 5 ATGGGAG 3295 0.0 34.330666 5 CATGGGG 3380 0.0 32.76275 4 GAGTACA 7695 0.0 31.970627 1 AGTACAT 7440 0.0 31.688839 2 ATGGGAC 1805 0.0 31.335054 5 ACGCAGA 8355 0.0 30.761368 8 CGCAGAG 8365 0.0 30.582352 9 GGGACCG 275 1.8189894E-12 28.124125 7 >>END_MODULE