FastQCFastQC Report
Thu 26 May 2016
SRR936264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4247942
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG122510.28839847625038195No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG99450.2341133659546199No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA84440.19877860855915644No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA84170.19814300666063708No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA82140.19336422201621398No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA70120.16506816712657565No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA65920.15518102648294163No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65480.1541452307964657No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA63020.1483541912766229No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG60850.14324583527741197No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA58350.1373606325133441No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG53780.12660248186062803No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT51590.12144704423930458No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG51140.12038770774177236No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA48450.11405522956763534No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT48130.11330192361383466No Hit
CCTCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAAC44490.10473306838935184No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT42560.10018969185549144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.064.849161
TACATGG199200.048.7121732
GTACATG204800.048.5421941
ACATGGG198000.048.256213
GTATCAA58400.044.6204722
ATCAACG62550.041.469814
CATGGGG108300.041.365614
CAACGCA62650.041.023776
TCAACGC64100.040.0957765
AACGCAG67250.038.306157
CATGGGA89950.036.0469254
TATCAAC76900.033.8860243
GAGTACA117950.031.071031
ACGCAGA83750.029.9071668
ATGGGGG51000.029.5136725
CGCAGAG83450.029.373049
CGATACC11850.027.86459118-119
ATGGGGA61000.027.016115
CATGGGC45450.026.7035624
ATGGGAG45600.026.3547825
Produced by FastQC (version 0.11.5)