FastQCFastQC Report
Thu 26 May 2016
SRR936264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4247942
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG129700.3053243193998411No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG85560.20141517939745884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79170.18637260113250134No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA76290.17959284754829513No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA75780.1783922661844253No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA70080.16497400388235056No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG64640.15216780266773886No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA61630.14508201853980116No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA60820.14317521284424317No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55880.13154605218244506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55220.12999235865273115No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA54610.12855636917829857No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA54270.12775598160238535No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG54070.12728516538125992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.12387174777810056No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT52250.12300073776901851No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT51620.12151766667247339No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG43190.10167276295203653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70650.083.815831
GTATCAA114550.051.506762
TACATGG177100.043.6356852
ACATGGG176950.043.5712243
GTACATG184200.042.5939521
ATCAACG155150.039.2174844
TCAACGC156400.038.9801755
CAACGCA156350.038.964646
AACGCAG160800.037.9973227
TATCAAC165450.036.8839723
CATGGGG96250.035.381474
CATGGGA79050.032.6111874
ACGCAGA190100.031.5774978
CGCAGAG190900.031.2581779
GAGTACA113300.027.236521
CAGAGTA213700.026.7539259
GCAGAGT226000.025.1662278
AGAGTAC205650.024.89377810-11
GTGGTAT25350.023.7353251
ATGGGGG46150.023.6080425
Produced by FastQC (version 0.11.5)