FastQCFastQC Report
Thu 26 May 2016
SRR936263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879991
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC48280.16763941276205377No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG40770.14156294238419495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.059.5645751
GTACATG230950.055.5141451
TACATGG236050.053.5586132
ACATGGG236600.052.0762563
CATGGGG118000.050.8725554
ATGGGGG82350.047.8987665
GTATCAA58100.043.212632
CATGGGA114050.042.46234
TGGGGGG80450.040.5255476
CGTACAC8050.039.170076
ATCAACG69600.036.1580924
TCAACGC70400.035.578195
ATGGGAG47650.035.459275
CAACGCA72250.034.7495356
TACCTGG27400.033.655462
AACGCAG75700.033.0872467
TATCAAC81600.031.642733
GAGTACA134700.031.2708741
CATGGGT30450.031.2613034
ATGGGAT51900.030.9507245
Produced by FastQC (version 0.11.5)