FastQCFastQC Report
Thu 26 May 2016
SRR936263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879991
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.27975781868762783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61620.21395900195521445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57200.19861173177277291No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37430.12996568392054003No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG30090.1044794931650828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.077.234571
GTACATG201550.050.6988681
GTATCAA100400.050.0453032
ACATGGG206700.048.6744233
TACATGG208200.048.666872
CATGGGG102850.045.5538444
ATGGGGG74950.041.303455
CGTACAC8350.040.6121866
CATGGGA102000.039.163094
ATCAACG138100.037.4611134
TCAACGC139350.037.082365
CAACGCA141850.036.5305986
AACGCAG146000.035.5737277
TATCAAC147200.035.38793
CCGTTGC4050.035.2552839
TTCGTAC9700.034.3693164
ATATTCG10000.033.366061
TACCTGG26350.032.9857862
ATGGGAG41050.032.775545
TGGGGGG79700.032.6951836
Produced by FastQC (version 0.11.5)