##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2879991 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56124654556212 31.0 31.0 34.0 30.0 34.0 2 31.329426723902955 31.0 31.0 34.0 28.0 34.0 3 31.702171638730814 31.0 31.0 34.0 30.0 34.0 4 35.54741316899948 37.0 35.0 37.0 33.0 37.0 5 35.180295355089655 37.0 35.0 37.0 33.0 37.0 6 35.393817897347596 37.0 35.0 37.0 33.0 37.0 7 35.22701598720274 37.0 35.0 37.0 32.0 37.0 8 35.30162559535776 37.0 35.0 37.0 32.0 37.0 9 36.95079290178337 39.0 37.0 39.0 33.0 39.0 10-11 36.84417781166677 39.0 37.0 39.0 32.0 39.0 12-13 36.77349668801048 39.0 37.0 39.0 32.0 39.0 14-15 37.92810758783621 40.0 37.0 41.0 32.5 41.0 16-17 37.8315442652425 40.0 37.0 41.0 32.0 41.0 18-19 37.93071141541762 40.0 37.0 41.0 32.0 41.0 20-21 37.974178565141344 40.0 37.0 41.0 32.5 41.0 22-23 37.898459057684555 40.0 37.0 41.0 32.0 41.0 24-25 37.87651471827516 40.0 37.0 41.0 32.0 41.0 26-27 37.70480359140011 40.0 37.0 41.0 32.0 41.0 28-29 37.555359200775285 40.0 36.5 41.0 32.0 41.0 30-31 37.38833576216037 39.5 36.0 41.0 31.5 41.0 32-33 37.182768626707514 39.0 36.0 41.0 31.0 41.0 34-35 37.010700207049254 39.0 36.0 41.0 31.0 41.0 36-37 36.84910543123225 39.0 35.0 41.0 30.0 41.0 38-39 36.85712177572778 39.0 35.0 41.0 30.5 41.0 40-41 36.90824381048413 39.0 35.0 41.0 31.0 41.0 42-43 36.83424739868978 40.0 35.0 41.0 31.0 41.0 44-45 36.69664367006702 40.0 35.0 41.0 30.0 41.0 46-47 36.46019935478965 39.5 35.0 41.0 30.0 41.0 48-49 36.22403177648819 39.0 35.0 41.0 29.5 41.0 50-51 36.04039908458047 39.0 35.0 41.0 29.0 41.0 52-53 35.89566373644918 39.0 35.0 41.0 28.5 41.0 54-55 35.708993535049245 39.0 35.0 41.0 28.0 41.0 56-57 35.520457529207555 39.0 35.0 40.5 28.0 41.0 58-59 35.30246275075166 38.0 34.5 40.0 27.5 41.0 60-61 35.0576307356516 38.0 34.0 40.0 27.0 41.0 62-63 34.8053113707647 37.5 34.0 40.0 26.0 41.0 64-65 34.5599746665875 37.0 34.0 40.0 26.0 41.0 66-67 34.278957989799274 37.0 34.0 39.5 26.0 41.0 68-69 33.94945001564241 36.0 33.0 39.0 26.0 41.0 70-71 33.62180958204384 36.0 33.0 39.0 26.0 41.0 72-73 33.36182248486193 35.0 33.0 38.5 26.0 40.0 74-75 33.17533943682463 35.0 33.0 37.5 26.0 39.5 76-77 32.87041522004756 35.0 33.0 37.0 26.0 39.0 78-79 32.55086699923715 35.0 33.0 36.5 26.0 39.0 80-81 32.28840940822384 35.0 33.0 36.0 25.5 38.5 82-83 32.044169408862736 35.0 32.5 36.0 25.0 37.0 84-85 31.80567265661594 35.0 32.0 35.0 25.0 37.0 86-87 31.620591696293495 35.0 32.0 35.0 25.0 36.5 88-89 31.487349960468627 35.0 32.0 35.0 25.0 36.0 90-91 31.361591581362582 35.0 32.0 35.0 24.0 36.0 92-93 31.230322941981417 35.0 32.0 35.0 24.0 36.0 94-95 31.11982520084264 35.0 32.0 35.0 24.0 35.0 96-97 31.055997223602436 35.0 32.0 35.0 23.0 35.0 98-99 30.988890243059785 35.0 32.0 35.0 23.0 35.0 100-101 30.919802006325714 34.5 32.0 35.0 23.0 35.0 102-103 29.88662013179902 33.5 30.0 34.5 19.5 35.0 104-105 30.647722510243955 34.0 31.5 35.0 20.5 35.0 106-107 30.80120927461232 34.0 32.0 35.0 23.0 35.0 108-109 30.775861799568123 34.5 32.0 35.0 20.0 35.0 110-111 30.747704246297992 35.0 32.0 35.0 20.0 35.0 112-113 30.670036468863966 34.0 32.0 35.0 19.5 35.0 114-115 30.6425532232566 35.0 32.0 35.0 19.0 35.0 116-117 30.542216277759202 34.0 32.0 35.0 19.0 35.0 118-119 30.415863973186028 34.0 32.0 35.0 18.0 35.0 120-121 30.216424113825354 34.0 32.0 35.0 11.0 35.0 122-123 29.98791853863432 34.0 32.0 35.0 2.0 35.0 124-125 27.858795912903894 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13237413537503784 1101 2 0.512865778121256 1101 3 0.3256705407385283 1101 4 0.19677623665560873 1101 5 0.4981842270159902 1101 6 0.2883339453473255 1101 7 0.38901029810284626 1101 8 0.3279850993991289 1101 9 0.20827888545525042 1101 10-11 0.3432575767817241 1101 12-13 0.32411339222487356 1101 14-15 0.3741336929241186 1101 16-17 0.47022267939559015 1101 18-19 0.4475615689870338 1101 20-21 0.37019981076974773 1101 22-23 0.3091694015309656 1101 24-25 0.3166979509766463 1101 26-27 0.26743886817867235 1101 28-29 0.38769818323505234 1101 30-31 0.2922945472993064 1101 32-33 0.20975254780164931 1101 34-35 0.30376004712748284 1101 36-37 0.3547522313163398 1101 38-39 0.29394536068420507 1101 40-41 0.25196016157767787 1101 42-43 0.23987697970401456 1101 44-45 0.2762367030151722 1101 46-47 0.2684995167152593 1101 48-49 0.29117203785233414 1101 50-51 0.27795379881695226 1101 52-53 0.15825109906010937 1101 54-55 0.2140515036170072 1101 56-57 0.0933719752479476 1101 58-59 0.160395441819972 1101 60-61 0.16967248969775994 1101 62-63 0.15832587732146663 1101 64-65 0.16132166616140609 1101 66-67 0.14525212070943638 1101 68-69 0.13134826270382405 1101 70-71 0.08170993178749342 1101 72-73 0.08407596985207988 1101 74-75 0.13114165085008267 1101 76-77 0.11286720941613027 1101 78-79 0.19402301171142255 1101 80-81 0.18797880574536663 1101 82-83 0.1752290234665992 1101 84-85 0.16604464264381846 1101 86-87 0.1861799387296017 1101 88-89 0.17877574536737484 1101 90-91 0.22984360552719707 1101 92-93 0.28206253250227675 1101 94-95 0.20630770599096948 1101 96-97 0.19132316037271835 1101 98-99 0.1946624885163466 1101 100-101 0.17964995635085756 1101 102-103 0.3021359827933523 1101 104-105 0.1934381214691676 1101 106-107 0.26795729578367045 1101 108-109 0.18840481222106575 1101 110-111 0.2583113083448829 1101 112-113 0.20380897276038112 1101 114-115 0.13284971917221355 1101 116-117 0.1530585849399877 1101 118-119 0.2674996538770209 1101 120-121 0.31970758327045345 1101 122-123 0.3140816586443975 1101 124-125 0.35204030813136455 1102 1 0.15496341550731074 1102 2 0.5531224612925492 1102 3 0.31151980432086646 1102 4 0.1598944855779436 1102 5 0.279690647590094 1102 6 0.25171052000822414 1102 7 0.4326868606871841 1102 8 0.38194819095148347 1102 9 0.346306569897493 1102 10-11 0.38375253899297235 1102 12-13 0.32238093031942583 1102 14-15 0.34229099262118723 1102 16-17 0.42755694610579553 1102 18-19 0.3945632915469375 1102 20-21 0.42537303366146517 1102 22-23 0.4479708497083621 1102 24-25 0.3407909762011201 1102 26-27 0.3483129294786593 1102 28-29 0.3980934400599594 1102 30-31 0.35145026772426746 1102 32-33 0.34836925570076005 1102 34-35 0.36849923423919506 1102 36-37 0.35311155707937303 1102 38-39 0.36621332087582914 1102 40-41 0.2248408129507311 1102 42-43 0.2571150533259825 1102 44-45 0.3002673068003787 1102 46-47 0.26356720368931263 1102 48-49 0.1899160660718664 1102 50-51 0.29556819702573733 1102 52-53 0.30441245236467296 1102 54-55 0.29219872851319906 1102 56-57 0.2620734869922998 1102 58-59 0.3026871512820506 1102 60-61 0.3061363860304098 1102 62-63 0.2532223858758229 1102 64-65 0.26576418931558976 1102 66-67 0.20207082322108505 1102 68-69 0.20850038501334467 1102 70-71 0.2843920538601452 1102 72-73 0.2863304613804303 1102 74-75 0.32080977654104004 1102 76-77 0.28411557412974986 1102 78-79 0.28111679181787963 1102 80-81 0.3079964002424944 1102 82-83 0.25136196773541286 1102 84-85 0.2116987902038261 1102 86-87 0.19779406395380406 1102 88-89 0.1888404500916394 1102 90-91 0.21584067104000226 1102 92-93 0.26593377425594866 1102 94-95 0.20162702029097446 1102 96-97 0.24285744634329376 1102 98-99 0.24328534342251373 1102 100-101 0.2523398679978257 1102 102-103 0.26708459723155187 1102 104-105 0.29383732744516067 1102 106-107 0.25909019927954446 1102 108-109 0.2533205364825619 1102 110-111 0.22719771569301983 1102 112-113 0.2685914568579655 1102 114-115 0.21441954167787003 1102 116-117 0.17757040657038559 1102 118-119 0.15898706843342936 1102 120-121 0.33358611173210306 1102 122-123 0.34451672151433854 1102 124-125 0.2246980965213261 1103 1 0.08565695918245098 1103 2 0.4472735957749734 1103 3 0.23865798720138187 1103 4 0.17776555384164539 1103 5 0.32342363035375143 1103 6 0.18845879150495648 1103 7 0.2024735974677867 1103 8 0.1850694296309996 1103 9 0.22477925467692472 1103 10-11 0.28119328297972146 1103 12-13 0.3181964917178064 1103 14-15 0.33997645371769636 1103 16-17 0.37647232248129114 1103 18-19 0.3843232486995305 1103 20-21 0.29030048521468643 1103 22-23 0.27041003560766796 1103 24-25 0.21324262680001027 1103 26-27 0.15940096141945048 1103 28-29 0.17202167065690332 1103 30-31 0.06058426324476329 1103 32-33 0.13990786705532798 1103 34-35 0.08926415779552599 1103 36-37 0.0011025980765424492 1103 38-39 -0.010213010802040401 1103 40-41 -0.06338286997847575 1103 42-43 -0.009853882305534967 1103 44-45 0.0237502165286827 1103 46-47 0.009500205929064975 1103 48-49 -0.08369528849041075 1103 50-51 -0.07309282157392971 1103 52-53 -0.10402946018766812 1103 54-55 -0.041114252302847376 1103 56-57 -0.09610958828280047 1103 58-59 -0.10621731848131333 1103 60-61 -0.1556808308358839 1103 62-63 -0.1975208218367115 1103 64-65 -0.08016482032508065 1103 66-67 -0.13233671402694114 1103 68-69 -0.1703171239943515 1103 70-71 -0.11913263208785452 1103 72-73 -0.08980071010656587 1103 74-75 -0.057840724968357904 1103 76-77 -0.04702650396938424 1103 78-79 -0.06261229572716331 1103 80-81 -0.05937072411236244 1103 82-83 -0.13002249034229862 1103 84-85 -0.15742254585615711 1103 86-87 -0.1949049330274022 1103 88-89 -0.08151800740178672 1103 90-91 -0.04267758779727515 1103 92-93 -0.08083718688924435 1103 94-95 -0.06383417359353771 1103 96-97 -0.09440499401117108 1103 98-99 -0.0904877600929197 1103 100-101 -0.11332601962512001 1103 102-103 -0.04024338017617879 1103 104-105 -0.14767791259768615 1103 106-107 -0.14854238422420707 1103 108-109 -0.09486565496743538 1103 110-111 -0.07129298447252452 1103 112-113 -0.056623656590279126 1103 114-115 0.11843200637745355 1103 116-117 0.08871988558489718 1103 118-119 0.0910264101414846 1103 120-121 -0.0914603387499291 1103 122-123 0.0014164196348929181 1103 124-125 0.09479878792263108 1104 1 0.06364160636284666 1104 2 0.4261317790246544 1104 3 0.22136067394481174 1104 4 0.15968741192648395 1104 5 0.3922568160638207 1104 6 0.20565768925124672 1104 7 0.3161080822625877 1104 8 0.24820908124009833 1104 9 0.28327408786263675 1104 10-11 0.2555943261841378 1104 12-13 0.23992466807840884 1104 14-15 0.21821845745748192 1104 16-17 0.41041553673186115 1104 18-19 0.28917464127899706 1104 20-21 0.2560041561132209 1104 22-23 0.24319499771769415 1104 24-25 0.20245490905570307 1104 26-27 0.20466747062130253 1104 28-29 0.21045676562786753 1104 30-31 0.16244500529771244 1104 32-33 0.09996199558598562 1104 34-35 0.08328147892533622 1104 36-37 0.099008316017688 1104 38-39 0.1864770787560701 1104 40-41 0.06942010216993566 1104 42-43 0.1077527508998557 1104 44-45 0.19397962308316608 1104 46-47 0.18656850629320587 1104 48-49 0.20368636685847719 1104 50-51 0.2530015589004364 1104 52-53 0.27047944641601873 1104 54-55 0.251276162026457 1104 56-57 0.19008104256062097 1104 58-59 0.22915912084552303 1104 60-61 0.25592422772134427 1104 62-63 0.1658644748860496 1104 64-65 0.15933473680512833 1104 66-67 0.13807604062319 1104 68-69 0.10915714034970847 1104 70-71 0.12024882643000723 1104 72-73 0.0989261868523954 1104 74-75 0.1122855421313318 1104 76-77 0.18040554012076626 1104 78-79 0.21140118719775103 1104 80-81 0.13273310425099538 1104 82-83 0.1280675598496117 1104 84-85 0.1480009360845962 1104 86-87 0.2141560845889714 1104 88-89 0.16474062855924032 1104 90-91 0.09991969593464134 1104 92-93 0.15439103867910475 1104 94-95 0.16441343983034074 1104 96-97 0.15920803620238644 1104 98-99 0.09946687035684221 1104 100-101 0.07386392230973371 1104 102-103 0.14261450782696983 1104 104-105 0.08167746580995328 1104 106-107 0.10859529907468257 1104 108-109 0.06673320341264599 1104 110-111 0.13961332396168658 1104 112-113 0.10786271286154658 1104 114-115 0.09872912280300028 1104 116-117 0.11576338524044161 1104 118-119 0.1068685516629877 1104 120-121 0.06281955496132241 1104 122-123 0.1641383958183411 1104 124-125 0.2517330463617604 1105 1 0.17981019564216183 1105 2 0.4977468087501684 1105 3 0.1949160233088243 1105 4 0.11291365306372114 1105 5 0.34733556342288807 1105 6 0.2649689925911147 1105 7 0.28681529715954923 1105 8 0.23358638251519181 1105 9 0.2348083167641164 1105 10-11 0.09910932583896681 1105 12-13 0.15100729459466322 1105 14-15 0.1366645500042054 1105 16-17 0.2732286706392273 1105 18-19 0.2344714946998252 1105 20-21 0.0941306628163261 1105 22-23 0.15413512000514373 1105 24-25 0.07882871222493293 1105 26-27 0.05824493617360105 1105 28-29 0.0898312993080097 1105 30-31 0.07463548884288684 1105 32-33 0.042998282437679336 1105 34-35 0.030964583452366412 1105 36-37 0.03338426547710327 1105 38-39 0.00418872118093816 1105 40-41 -0.08608755220362951 1105 42-43 -0.1633423602154096 1105 44-45 -0.0960930243343654 1105 46-47 -0.10721739277928322 1105 48-49 -0.1348944665323799 1105 50-51 -0.07246020745386517 1105 52-53 -0.04033431854668379 1105 54-55 -0.09880807293986038 1105 56-57 -0.13266631806409634 1105 58-59 -0.08541811108369757 1105 60-61 -0.17870270079038875 1105 62-63 -0.22209677956458762 1105 64-65 -0.1532141378063656 1105 66-67 -0.12416674485037049 1105 68-69 -0.17215493406333593 1105 70-71 -0.10167116137010623 1105 72-73 -0.09109133097842914 1105 74-75 -0.058127089271899024 1105 76-77 -0.05114788955577154 1105 78-79 -0.03622406734025674 1105 80-81 -0.011790950150817991 1105 82-83 -0.04366934194952421 1105 84-85 -0.08611196314663516 1105 86-87 -0.09161313792979442 1105 88-89 -0.17037798098170853 1105 90-91 -0.10741074499674497 1105 92-93 -0.12214321947400819 1105 94-95 -0.13665202182485459 1105 96-97 -0.1997923417287737 1105 98-99 -0.19638721701350903 1105 100-101 -0.15593265400057277 1105 102-103 -0.16243595319834014 1105 104-105 -0.16894754418435554 1105 106-107 -0.15542510842793433 1105 108-109 -0.18787563588626455 1105 110-111 -0.18776120698830212 1105 112-113 -0.22223821698951696 1105 114-115 -0.2529364069500133 1105 116-117 -0.27680748855278026 1105 118-119 -0.20305094197945905 1105 120-121 -0.17115298516119992 1105 122-123 -0.2707956748902802 1105 124-125 -0.03563611593763483 1106 1 0.15134209550610933 1106 2 0.5544791073551565 1106 3 0.3032261490307562 1106 4 0.14194262801344593 1106 5 0.36136382384540155 1106 6 0.22979798822146336 1106 7 0.3182431665091201 1106 8 0.23133504088870183 1106 9 0.16486016936470094 1106 10-11 0.2245058013028043 1106 12-13 0.2143207954410613 1106 14-15 0.1821977964324475 1106 16-17 0.23286253063140094 1106 18-19 0.19862110856467297 1106 20-21 0.13811344329149478 1106 22-23 0.13623911798820387 1106 24-25 0.17945810613159097 1106 26-27 0.23092045716850151 1106 28-29 0.05894310132239866 1106 30-31 -0.018239542090910277 1106 32-33 -0.018004768535405447 1106 34-35 -0.030360585681457053 1106 36-37 -0.06795486307854048 1106 38-39 0.016228795119978656 1106 40-41 0.02098710949968563 1106 42-43 0.09103324756840436 1106 44-45 0.0019313950687092074 1106 46-47 -0.07396036281910057 1106 48-49 -0.03401484287328316 1106 50-51 0.0027485739112691476 1106 52-53 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46-47 -0.7176489886904633 2216 48-49 -0.8139594822646998 2216 50-51 -0.6300591815439844 2216 52-53 -0.7460501561716342 2216 54-55 -0.9413201012728791 2216 56-57 -1.41399393709586 2216 58-59 -1.4778137105699898 2216 60-61 -1.025978688789472 2216 62-63 -1.0296925236406693 2216 64-65 -0.9027023121746751 2216 66-67 -0.8535442776000792 2216 68-69 -0.7525589796295975 2216 70-71 -0.7459228226261416 2216 72-73 -0.848272274259287 2216 74-75 -0.8512441638343091 2216 76-77 -0.6284640417027276 2216 78-79 -0.5698602611453047 2216 80-81 -0.569475459797836 2216 82-83 -0.6308850564511133 2216 84-85 -0.6941735661657518 2216 86-87 -0.6312940996336991 2216 88-89 -0.6433591288971137 2216 90-91 -0.635247101671073 2216 92-93 -0.6172646301986724 2216 94-95 -0.6279046413662392 2216 96-97 -0.6246163490306138 2216 98-99 -0.7829615942835559 2216 100-101 -0.9355409245974684 2216 102-103 -0.923435777964098 2216 104-105 -0.7186318115085513 2216 106-107 -0.5891991496616704 2216 108-109 -0.633079835459597 2216 110-111 -0.6888184982497236 2216 112-113 -0.5524416188690111 2216 114-115 -0.5849517749261324 2216 116-117 -0.5863443275088756 2216 118-119 -0.6597634423060512 2216 120-121 -0.7296568177762275 2216 122-123 -0.795460660226972 2216 124-125 -0.9090356200563114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111.0 3 106.0 4 291.0 5 544.0 6 1371.0 7 2766.0 8 4329.0 9 7250.0 10 11637.0 11 14277.0 12 14930.0 13 18560.0 14 24848.0 15 19147.0 16 10354.0 17 9289.0 18 8801.0 19 8658.0 20 8692.0 21 9241.0 22 10601.0 23 12714.0 24 16332.0 25 21380.0 26 27942.0 27 36580.0 28 46813.0 29 59497.0 30 75225.0 31 94177.0 32 119848.0 33 153124.0 34 211952.0 35 287013.0 36 477355.0 37 767265.0 38 285535.0 39 1436.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.6339881929994 17.74434240649201 10.361336190287906 23.260333210220686 2 19.178889846071794 20.700150938600594 37.484578880609895 22.636380334717717 3 17.91062396418875 27.461563221248415 31.446995110050768 23.180817704512066 4 11.83455909032986 15.97110266740602 36.65114693146398 35.54319131080014 5 10.581282020545268 41.71856839823004 34.93244522738605 12.767704353838644 6 31.397400685419047 36.384431890388505 18.504161472782894 13.714005951409552 7 27.468731673119812 34.28253768848583 21.957082504771716 16.29164813362264 8 27.715086609645656 33.46232679199345 20.076451627800225 18.74613497056067 9 28.545610038364703 11.626459943798436 18.91262854640865 40.91530147142821 10-11 25.04570326782271 24.853428361408074 29.944746355110137 20.156122015659076 12-13 25.882424632576978 24.13922474063287 29.35899799686874 20.619352629921416 14-15 23.30302421083955 25.786677805590365 26.915222998960758 23.995074984609328 16-17 22.472620555147955 27.764654656667094 27.355607955447052 22.407116832737902 18-19 21.62503285600545 26.739441199642638 29.853895376756384 21.78163056759552 20-21 22.568542748918315 26.04995987834684 29.982680501432124 21.398816871302724 22-23 22.214513864800274 26.13525528378387 29.940423424934316 21.709807426481543 24-25 22.099586786831416 26.096995806062555 29.64662557046629 22.156791836639737 26-27 21.56194238106994 26.42232215309006 29.892905915330985 22.122829550509014 28-29 21.937350476911377 26.894381577713812 29.389668019680627 21.778599925694188 30-31 22.857154761941963 25.866000970141922 29.85099606214047 21.425848205775644 32-33 22.417188109268395 26.038310536387094 29.73052693567445 21.81397441867006 34-35 22.5538383600062 26.655055501128217 29.353557733690767 21.437548405174816 36-37 22.35119831971697 26.628954743261353 29.28451859745395 21.73532833956773 38-39 22.512275211971147 26.57763513844314 29.222261458455947 21.687828191129764 40-41 22.4891501397053 26.3028599742152 29.233216353801105 21.974773532278398 42-43 22.11436411936392 26.350408544972936 28.892062978931154 22.643164356731997 44-45 21.927021975360304 26.615943834475956 29.06072762724622 22.396306562917523 46-47 22.024187276633363 26.45787888790131 28.32374508816943 23.1941887472959 48-49 22.360529165130053 26.671594253024438 28.35081081193705 22.617065769908457 50-51 22.42920992712207 26.53993562628939 28.64927023103071 22.381584215557826 52-53 22.488089450656297 26.54371831377179 28.403708590874366 22.564483644697546 54-55 22.031281279586747 26.47656143790475 28.897932424199336 22.59422485830917 56-57 22.639453831147684 26.719538780252527 28.000806988716743 22.64020039988305 58-59 22.677165709769657 26.864160903842745 27.98851272179081 22.47016066459679 60-61 23.468088648882514 26.475112967410418 28.261549308514617 21.795249075192448 62-63 22.611494161875783 26.52829963308378 28.558960592704942 22.301245612335496 64-65 22.36862413663371 26.279721389400134 28.529964320356704 22.82169015360945 66-67 22.60057296899575 26.93443878396958 27.902784512649088 22.562203734385587 68-69 22.298581453283376 26.532797653574043 28.50991222257157 22.65870867057101 70-71 22.135010792870126 26.394327184820497 28.73999035733923 22.730671664970146 72-73 22.270852062158692 27.12951301755362 27.796510943536955 22.803123976750737 74-75 22.085191497621242 27.131649438770296 27.95025157375744 22.832907489851024 76-77 21.849821291882517 26.587101174992632 28.13331224519097 23.429765287933872 78-79 22.206799712290618 26.62653804814219 28.30253374187649 22.864128497690704 80-81 22.292577624644053 26.670002336820968 27.892944432619522 23.144475605915453 82-83 22.541843483055573 26.936965199687428 27.94339282951998 22.577798487737017 84-85 22.25291681054755 26.931341518441144 27.69886247730675 23.116879193704555 86-87 21.770543636417614 27.21701203009529 27.80659833183188 23.205846001655217 88-89 21.94095090573322 26.68134742867044 28.07284269101701 23.30485897457933 90-91 22.583614531125974 26.53096833200113 27.987664334906743 22.89775280196615 92-93 22.40430878067014 26.558053625034482 27.83509396507857 23.202543629216805 94-95 22.21992666206127 26.579125962189348 27.72032415084191 23.48062322490747 96-97 21.900031337322403 26.74724669938133 27.711290377185037 23.64143158611123 98-99 21.656536854137645 26.689631201674473 27.61787712833704 24.03595481585084 100-101 21.757860229939656 26.805268660504805 27.72678771641262 23.710083393142924 102-103 21.67455476555931 26.538422342026646 27.621297641313465 24.165725251100575 104-105 21.818933403380274 26.50480985328511 27.476381293091762 24.199875450242857 106-107 21.700245036508946 26.444619908686835 27.800185348573724 24.054949706230495 108-109 21.958224166843607 26.397633444694584 27.58858479777456 24.05555759068725 110-111 21.804711651209892 27.087723574610408 27.24756915617436 23.859995618005346 112-113 21.679548629227803 26.832617250746395 27.463886952422538 24.02394716760326 114-115 21.728993222208484 26.971762211902185 27.146173305309013 24.153071260580315 116-117 21.553895287092622 26.743090877645553 27.362270763832306 24.340743071429515 118-119 21.35724810332392 26.34988087064959 27.587117569782087 24.705753456244402 120-121 21.420488066412645 26.64110482173669 27.443986814421628 24.494420297429034 122-123 21.42323870030846 26.66321965725985 27.05020954645667 24.86333209597502 124-125 21.31688443144598 26.488478910629194 26.783131975786745 25.41150468213808 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 87.0 1 75.0 2 258.0 3 549.5 4 521.5 5 491.0 6 689.0 7 1357.0 8 2172.0 9 2718.0 10 2691.0 11 2350.5 12 2465.5 13 2663.0 14 2871.0 15 3144.0 16 3426.0 17 3791.0 18 4202.5 19 4569.5 20 5128.0 21 5982.0 22 7166.0 23 8646.5 24 10786.0 25 13557.0 26 17276.5 27 21496.5 28 25171.0 29 29198.0 30 34706.5 31 40473.5 32 46602.0 33 52858.0 34 59622.5 35 65347.0 36 71747.0 37 77828.5 38 78786.5 39 79851.0 40 80562.5 41 81897.5 42 83822.5 43 87491.0 44 94283.5 45 98552.0 46 101047.0 47 104189.0 48 107869.0 49 109875.5 50 113495.5 51 113333.0 52 109243.5 53 107509.5 54 102780.5 55 93946.0 56 84667.5 57 77417.5 58 68931.5 59 58077.5 60 45953.0 61 36059.0 62 29369.0 63 22556.0 64 17130.0 65 13608.0 66 10467.5 67 8143.0 68 6394.0 69 4552.5 70 3202.0 71 2501.0 72 2129.5 73 1716.5 74 1256.5 75 963.5 76 638.5 77 446.5 78 284.0 79 126.0 80 86.5 81 54.5 82 26.0 83 15.5 84 15.5 85 12.0 86 3.5 87 4.0 88 3.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06486131380271674 2 4.16667968754069E-4 3 6.94446614590115E-5 4 6.94446614590115E-5 5 0.06791687890691325 6 3.472233072950575E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.94446614590115E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.7361165364752875E-5 26-27 0.0 28-29 3.472233072950575E-5 30-31 0.0 32-33 0.0 34-35 1.562504882827759E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0026215359700776843 44-45 0.01001739241546241 46-47 0.011684064290478685 48-49 0.00989586425790914 50-51 0.008472248697999403 52-53 0.0066319651693355994 54-55 0.007552106933667502 56-57 0.005173627278696357 58-59 0.0038020952148808797 60-61 0.002343757324241638 62-63 0.0014583378906392417 64-65 0.0019965340169465808 66-67 0.0027604252929957075 68-69 0.0012673650716269601 70-71 0.002795147623725213 72-73 0.0025000078125244143 74-75 7.118077799548679E-4 76-77 5.381961263073392E-4 78-79 5.2083496094258625E-5 80-81 3.4722330729505753E-4 82-83 8.680582682376438E-5 84-85 1.562504882827759E-4 86-87 1.9097281901228166E-4 88-89 0.001093753417979431 90-91 0.0017013942057457817 92-93 3.9930680338931616E-4 94-95 0.0012326427408974542 96-97 0.0013368097330859715 98-99 0.0014930602213687474 100-101 6.250019531311036E-4 102-103 6.770854492253621E-4 104-105 6.076407877663506E-4 106-107 4.16667968754069E-4 108-109 8.506971028728908E-4 110-111 8.33335937508138E-4 112-113 0.011059062337347582 114-115 0.020382008138219877 116-117 0.01864589160174459 118-119 0.012083371093868001 120-121 0.006180574869852023 122-123 0.00694446614590115 124-125 0.0056771010742741905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2879991.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.464919731744583 #Duplication Level Percentage of deduplicated Percentage of total 1 39.76350589230512 10.125744858003028 2 17.234044979886594 8.777271441321757 3 12.684741243008437 9.690477527134798 4 8.879106907750682 9.044229787818 5 6.131401045806646 7.806781773730052 6 4.168791935104481 6.3694771203467875 7 2.7460013812646302 4.89486933300145 8 1.8475759976825732 3.763869958342772 9 1.226010293604631 2.8098228345241085 >10 4.836321025283284 21.068367759700706 >50 0.29890895669549294 5.280993666671634 >100 0.1754741397047037 8.067657922948557 >500 0.006327886229414639 1.0366849927077533 >1k 0.001375627441172137 0.5652830211284606 >5k 4.126882323516411E-4 0.698468002620089 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8057 0.27975781868762783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6162 0.21395900195521445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5720 0.19861173177277291 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 3743 0.12996568392054003 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 3009 0.1044794931650828 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.7361165364752875E-5 0.0 20-21 0.0 0.0 0.0 3.472233072950575E-5 0.0 22-23 0.0 0.0 0.0 1.0416699218851725E-4 0.0 24-25 0.0 0.0 0.0 2.77778645836046E-4 0.0 26-27 0.0 0.0 0.0 3.8194563802456327E-4 0.0 28-29 0.0 0.0 0.0 6.597242838606093E-4 0.0 30-31 0.0 0.0 0.0 0.00229167382814738 0.0 32-33 0.0 0.0 0.0 0.007239605957101949 0.0 34-35 0.0 0.0 0.0 0.013923654622531808 0.0 36-37 0.0 0.0 0.0 0.02552091308618673 0.0 38-39 0.0 0.0 0.0 0.046788340658009 0.0 40-41 0.0 0.0 0.0 0.07533009651766273 0.0 42-43 0.0 0.0 0.0 0.10500032812602539 0.0 44-45 0.0 0.0 0.0 0.1400698821628262 0.0 46-47 0.0 0.0 0.0 0.18211862467625767 0.0 48-49 0.0 0.0 0.0 0.22932363330302075 0.0 50-51 0.0 0.0 0.0 0.2764591972683248 0.0 52-53 0.0 0.0 0.0 0.3289767224967023 0.0 54-55 0.0 0.0 0.0 0.4019630616901233 0.0 56-57 0.0 0.0 0.0 0.4846022088263471 0.0 58-59 0.0 0.0 0.0 0.5792552823949797 0.0 60-61 0.0 0.0 0.0 0.6774673948633867 0.0 62-63 0.0 0.0 0.0 0.7861482900467398 0.0 64-65 0.0 0.0 0.0 0.90449935433826 0.0 66-67 0.0 0.0 0.0 1.0406976966247465 0.0 68-69 0.0 0.0 0.0 1.2348476088987779 0.0 70-71 0.0 0.0 0.0 1.4416885330544436 0.0 72-73 0.0 0.0 0.0 1.6171231090652713 0.0 74-75 0.0 0.0 0.0 1.806134116391336 0.0 76-77 0.0 0.0 0.0 2.0530098878781216 0.0 78-79 0.0 0.0 0.0 2.3575073672105225 0.0 80-81 0.0 0.0 0.0 2.6407721413018304 0.0 82-83 0.0 0.0 0.0 2.9301306844361665 0.0 84-85 0.0 0.0 0.0 3.264228950715471 0.0 86-87 0.0 0.0 0.0 3.6390391497751207 0.0 88-89 0.0 0.0 0.0 4.048311262083805 0.0 90-91 0.0 0.0 0.0 4.460274355024026 0.0 92-93 0.0 0.0 0.0 4.877341630581485 0.0 94-95 0.0 0.0 0.0 5.328853458222612 0.0 96-97 0.0 0.0 0.0 5.821337636124557 0.0 98-99 0.0 0.0 0.0 6.399429720440098 0.0 100-101 0.0 0.0 0.0 6.99370241087559 0.0 102-103 0.0 0.0 0.0 7.576013258374766 0.0 104-105 0.0 0.0 0.0 8.181240844155415 0.0 106-107 0.0 0.0 0.0 8.890027781336816 0.0 108-109 0.0 0.0 0.0 9.603467510835973 0.0 110-111 0.0 0.0 0.0 10.356525419697492 0.0 112-113 0.0 0.0 0.0 11.055294964463432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6565 0.0 77.23457 1 GTACATG 20155 0.0 50.698868 1 GTATCAA 10040 0.0 50.045303 2 ACATGGG 20670 0.0 48.674423 3 TACATGG 20820 0.0 48.66687 2 CATGGGG 10285 0.0 45.553844 4 ATGGGGG 7495 0.0 41.30345 5 CGTACAC 835 0.0 40.612186 6 CATGGGA 10200 0.0 39.16309 4 ATCAACG 13810 0.0 37.461113 4 TCAACGC 13935 0.0 37.08236 5 CAACGCA 14185 0.0 36.530598 6 AACGCAG 14600 0.0 35.573727 7 TATCAAC 14720 0.0 35.3879 3 CCGTTGC 405 0.0 35.255283 9 TTCGTAC 970 0.0 34.369316 4 ATATTCG 1000 0.0 33.36606 1 TACCTGG 2635 0.0 32.985786 2 ATGGGAG 4105 0.0 32.77554 5 TGGGGGG 7970 0.0 32.695183 6 >>END_MODULE