FastQCFastQC Report
Thu 26 May 2016
SRR936262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147594
Sequences flagged as poor quality0
Sequence length125
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52933.586189140479965No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19071.2920579427347996No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17781.2046560158272017No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14921.0108812011328374No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9660.6544981503313143No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.42955675704974455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5620.38077428621759696No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.3719663400951258No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5170.35028524194750466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.32318386926297815No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4640.31437592314050705No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.24526742279496455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.19851755491415637No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2900.19648495196281693No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.18022412835210103No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2650.17954659403498788No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT2480.1680285106440641No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG2480.1680285106440641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.16193070179004568No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.15718796157025353No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2310.15651042725314038No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2050.13889453500819815No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT1810.12263371139748228No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1710.11585836822635066No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1660.11247069664078485No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC1520.1029852162012006No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.10027507893274794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGG950.0100.190552
TACAAAG701.8189894E-1276.484752
GAACAAA8900.074.861471
CGCAAAG401.9755662E-674.360172
GGGTATG250.002052147471.409977
GGAACGC250.002052147471.409978
GACTGCG250.002052147471.409977
GAAAACA5550.067.527081
GTACATA1150.062.074581
CCTGCAT150.004231095359.5083134-35
GGGATAC300.004226906759.508319
CCAGGAG300.004232586459.488143
CGACATG150.004236778759.4881480-81
CGCTGAT150.004236778759.4881462-63
GTACTAT300.004232586459.488141
GCTCCGT150.004236778759.48814116-117
GTCGGGA507.4287364E-659.488142
GGGAGTG401.7686411E-459.4881365
GGATATG1051.8189894E-1256.655371
GTACAAG2450.055.8460121
Produced by FastQC (version 0.11.5)