##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147594 Sequences flagged as poor quality 0 Sequence length 125 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.26566120574007 30.0 12.0 31.0 2.0 34.0 2 23.70674282152391 30.0 15.0 31.0 2.0 34.0 3 24.51042725314037 30.0 20.0 33.0 2.0 34.0 4 25.96863016111766 33.0 12.0 37.0 2.0 37.0 5 28.54849113107579 35.0 27.0 37.0 2.0 37.0 6 26.6183110424543 35.0 16.0 37.0 2.0 37.0 7 27.282328549941056 35.0 18.0 37.0 2.0 37.0 8 26.358720544195563 35.0 13.0 37.0 2.0 37.0 9 26.49430871173625 35.0 11.0 39.0 2.0 39.0 10-11 27.405558491537597 34.5 14.5 39.0 2.0 39.0 12-13 27.473572773961003 35.0 15.5 39.0 2.0 39.0 14-15 28.19252476387929 36.0 15.0 40.0 2.0 41.0 16-17 28.469724379039796 36.0 17.5 40.0 2.0 41.0 18-19 28.225317424827566 35.0 17.0 40.0 2.0 41.0 20-21 28.106755694675936 35.0 17.5 40.0 2.0 41.0 22-23 27.88601501416047 34.5 17.5 40.0 2.0 41.0 24-25 27.784435681667276 34.0 18.5 40.0 2.0 41.0 26-27 27.736215564318336 34.0 18.5 40.0 2.0 41.0 28-29 27.496090626990256 34.0 16.0 40.0 2.0 41.0 30-31 27.273418973671017 34.0 11.5 40.0 2.0 41.0 32-33 27.028910389311218 34.0 8.0 40.0 2.0 41.0 34-35 26.824203558410233 34.0 2.0 40.0 2.0 41.0 36-37 26.54015407130371 33.0 2.0 40.0 2.0 41.0 38-39 26.16821483258127 33.0 2.0 40.0 2.0 41.0 40-41 26.1098147621177 33.0 2.0 40.0 2.0 41.0 42-43 25.83092131116441 33.0 2.0 40.0 2.0 41.0 44-45 25.735046817621313 33.0 2.0 40.0 2.0 41.0 46-47 25.635276501754817 33.0 2.0 40.0 2.0 41.0 48-49 25.479596054040137 33.0 2.0 40.0 2.0 41.0 50-51 25.40064975541011 33.0 2.0 40.0 2.0 41.0 52-53 25.27832093445533 33.0 2.0 40.0 2.0 41.0 54-55 25.153068552922207 33.0 2.0 40.0 2.0 41.0 56-57 25.018666070436467 33.0 2.0 40.0 2.0 41.0 58-59 24.79955485995366 32.0 2.0 39.0 2.0 41.0 60-61 24.571327425234088 32.0 2.0 39.0 2.0 41.0 62-63 24.31313603534019 31.5 2.0 39.0 2.0 41.0 64-65 24.06586988630974 31.0 2.0 39.0 2.0 41.0 66-67 23.822015122565958 31.0 2.0 38.0 2.0 40.0 68-69 23.57127322248872 31.0 2.0 37.5 2.0 40.0 70-71 23.25155832892936 31.0 2.0 37.0 2.0 40.0 72-73 22.924177134571863 30.5 2.0 36.5 2.0 39.5 74-75 22.635483149721534 30.0 2.0 36.0 2.0 39.0 76-77 22.329820317899102 30.0 2.0 36.0 2.0 39.0 78-79 22.044798569047522 30.0 2.0 35.0 2.0 38.0 80-81 21.79065205902679 30.0 2.0 35.0 2.0 37.0 82-83 21.524990853286717 30.0 2.0 35.0 2.0 37.0 84-85 21.397133352304294 30.0 2.0 35.0 2.0 36.5 86-87 21.226069487919563 30.0 2.0 35.0 2.0 36.0 88-89 21.013686193205686 30.0 2.0 35.0 2.0 36.0 90-91 20.81985040042278 29.0 2.0 35.0 2.0 36.0 92-93 20.644057346504603 29.0 2.0 35.0 2.0 35.0 94-95 20.51248356979281 29.0 2.0 35.0 2.0 35.0 96-97 20.378450343509897 29.0 2.0 35.0 2.0 35.0 98-99 20.257940702196567 29.0 2.0 35.0 2.0 35.0 100-101 20.11612599428161 28.5 2.0 35.0 2.0 35.0 102-103 19.423943385232462 26.5 2.0 34.0 2.0 35.0 104-105 19.807881756710977 28.0 2.0 34.5 2.0 35.0 106-107 19.827052590213697 28.0 2.0 35.0 2.0 35.0 108-109 19.769225036248088 28.0 2.0 35.0 2.0 35.0 110-111 19.66528788433134 28.0 2.0 35.0 2.0 35.0 112-113 19.587432415951866 28.0 2.0 35.0 2.0 35.0 114-115 19.43535306313265 27.0 2.0 35.0 2.0 35.0 116-117 19.284550862501185 27.0 2.0 35.0 2.0 35.0 118-119 19.135618656584956 26.5 2.0 35.0 2.0 35.0 120-121 18.967979728173233 26.0 2.0 35.0 2.0 35.0 122-123 18.70145466617884 25.0 2.0 35.0 2.0 35.0 124-125 17.40624957654105 21.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5942409648485594 1101 2 1.1759121076196166 1101 3 1.291710680184341 1101 4 1.474221127942517 1101 5 0.9515715569660088 1101 6 0.9241489132370901 1101 7 0.3383112169911975 1101 8 0.5728219093515605 1101 9 0.771613484514404 1101 10-11 0.7024553566427798 1101 12-13 0.7712131723970046 1101 14-15 0.8543197028569836 1101 16-17 0.4453262990968163 1101 18-19 1.0013425350416796 1101 20-21 0.5870348406546455 1101 22-23 0.8108862705963666 1101 24-25 1.0441254166206413 1101 26-27 1.2567512750637952 1101 28-29 1.5335219177587547 1101 30-31 1.3133819331386256 1101 32-33 1.4442899933563353 1101 34-35 1.4176675020641838 1101 36-37 1.4016342870320706 1101 38-39 1.027855544208954 1101 40-41 1.3887998602313871 1101 42-43 1.533885036326776 1101 44-45 1.5278010661621053 1101 46-47 0.9998995298858873 1101 48-49 0.8434115800549229 1101 50-51 0.8777711679868787 1101 52-53 1.0378028052920243 1101 54-55 1.1300809596055679 1101 56-57 1.14873566660048 1101 58-59 1.1341775709052904 1101 60-61 0.9135288695383998 1101 62-63 1.3040852296890364 1101 64-65 1.3412796231403803 1101 66-67 1.4174722298421756 1101 68-69 1.5459304699014602 1101 70-71 1.3586163247750065 1101 72-73 1.304580376000601 1101 74-75 1.5742547322747313 1101 76-77 1.3623709829455066 1101 78-79 1.401409235036006 1101 80-81 1.4719469804887524 1101 82-83 1.435488163228026 1101 84-85 1.140613009904854 1101 86-87 1.0536779308102098 1101 88-89 1.2013767424180841 1101 90-91 1.2385305833172318 1101 92-93 1.5951954139088862 1101 94-95 1.4880003754609845 1101 96-97 1.3143220765717274 1101 98-99 1.2725624357005536 1101 100-101 1.5714386157163638 1101 102-103 1.0115659354313173 1101 104-105 0.6844876699378624 1101 106-107 0.8796973480222228 1101 108-109 1.1017554073325542 1101 110-111 1.1705424508374307 1101 112-113 1.4160067896628057 1101 114-115 1.4581189733306203 1101 116-117 1.4576198001898 1101 118-119 1.3342670270579262 1101 120-121 1.502550848771957 1101 122-123 1.3652349875796475 1101 124-125 0.9616802183133899 1102 1 0.660480281087878 1102 2 0.8667578116232164 1102 3 0.6809369509895618 1102 4 0.3148644027963208 1102 5 0.3648644044693796 1102 6 0.836204693713924 1102 7 0.47360136543924014 1102 8 1.3037036007595688 1102 9 0.7944429941860172 1102 10-11 1.1521292207903286 1102 12-13 0.38727255161428076 1102 14-15 0.9455815112240522 1102 16-17 0.7979466319803095 1102 18-19 0.42385715492472187 1102 20-21 0.11492507907119887 1102 22-23 0.5287787582256946 1102 24-25 0.2202950072639176 1102 26-27 0.21373508073181213 1102 28-29 0.2989852555905088 1102 30-31 0.5165420770882463 1102 32-33 0.6791078071215182 1102 34-35 0.5543071781775453 1102 36-37 0.4395335222997261 1102 38-39 0.23703233233311138 1102 40-41 0.48439140319135277 1102 42-43 0.7261364983150784 1102 44-45 0.8778348043537392 1102 46-47 1.1454235964625852 1102 48-49 1.040442619191225 1102 50-51 1.2443928503980324 1102 52-53 1.3330682123995352 1102 54-55 1.3056882470549631 1102 56-57 1.3186074614722756 1102 58-59 1.1007767611886905 1102 60-61 1.4842666113287741 1102 62-63 1.520965571569377 1102 64-65 1.7506363482865837 1102 66-67 1.6370516270531503 1102 68-69 1.629713645789895 1102 70-71 1.5455821367408156 1102 72-73 1.295414833940324 1102 74-75 1.0245471299355522 1102 76-77 1.0878545636922432 1102 78-79 1.1338653753868826 1102 80-81 1.2145808086488934 1102 82-83 1.354291582031447 1102 84-85 1.3628352321270754 1102 86-87 1.386673882227214 1102 88-89 1.5637811508751227 1102 90-91 1.4994392652785429 1102 92-93 1.3891675236704693 1102 94-95 1.664001275146095 1102 96-97 1.7276149240751018 1102 98-99 1.6499803394342472 1102 100-101 1.3223945311459637 1102 102-103 1.1375780811803047 1102 104-105 1.402380157567194 1102 106-107 1.590167433492308 1102 108-109 1.5143172606838817 1102 110-111 1.570958555200903 1102 112-113 1.3754647293839035 1102 114-115 1.5291378667179387 1102 116-117 1.660779944139417 1102 118-119 1.7426116064551422 1102 120-121 1.694521159163319 1102 122-123 1.1960941868598987 1102 124-125 1.1204746402657086 1103 1 -0.28182741121981536 1103 2 -0.6275066957991839 1103 3 -0.20401581554215298 1103 4 -0.5856079318865426 1103 5 -0.7236421182476676 1103 6 -0.5160220269338502 1103 7 -1.140321261641283 1103 8 -0.7391439026142521 1103 9 -0.984796771895855 1103 10-11 -0.3894249852375644 1103 12-13 -0.6411799899961537 1103 14-15 -0.49610764757036563 1103 16-17 -0.4093745556040389 1103 18-19 -0.6033583196591756 1103 20-21 -0.2206574670376611 1103 22-23 -0.8472333875232927 1103 24-25 -0.592626720131495 1103 26-27 -0.625727357414835 1103 28-29 -0.6416917574549217 1103 30-31 -0.269951400194703 1103 32-33 -0.36340231433597125 1103 34-35 -0.6207940363973563 1103 36-37 -0.643237507839725 1103 38-39 -0.6153068489534377 1103 40-41 -0.47658475515322607 1103 42-43 -0.5579953055535682 1103 44-45 -0.658096369735329 1103 46-47 -0.6859979060115506 1103 48-49 -0.6608619242185831 1103 50-51 -0.5581262679105592 1103 52-53 -0.863906596417376 1103 54-55 -1.2075258780012703 1103 56-57 -1.4922899744251623 1103 58-59 -1.2205232837955613 1103 60-61 -1.3492916432821112 1103 62-63 -1.4608720352682276 1103 64-65 -1.4685494366886758 1103 66-67 -1.5034679410979948 1103 68-69 -1.6399669659959777 1103 70-71 -1.7033494871908061 1103 72-73 -1.8984110769908469 1103 74-75 -1.6522409942209961 1103 76-77 -1.720962350387829 1103 78-79 -1.3464540128272375 1103 80-81 -1.5259162673744981 1103 82-83 -1.7824605547206929 1103 84-85 -1.7931476738558274 1103 86-87 -1.8330742059419265 1103 88-89 -1.9417856507443076 1103 90-91 -1.861896767110121 1103 92-93 -1.7680524493389775 1103 94-95 -1.5996064621458501 1103 96-97 -1.704908692659039 1103 98-99 -1.6782922651541483 1103 100-101 -1.7405271962494488 1103 102-103 -1.6721947483293675 1103 104-105 -1.8774781420279503 1103 106-107 -2.2442342759094025 1103 108-109 -1.9238856183084714 1103 110-111 -1.8102267799318028 1103 112-113 -2.1694632958072795 1103 114-115 -2.065385300173652 1103 116-117 -1.9462263536563533 1103 118-119 -1.6208611780702782 1103 120-121 -2.1512953050741963 1103 122-123 -2.2309188585741992 1103 124-125 -2.215670209037036 1104 1 0.8220744839864267 1104 2 0.6831956453379391 1104 3 0.814155867846921 1104 4 1.0732549443676405 1104 5 1.0913708880696866 1104 6 1.0500872261319252 1104 7 0.9494594890090333 1104 8 0.3065640126588818 1104 9 1.4794544358336168 1104 10-11 1.4916601132388365 1104 12-13 1.4413915447492904 1104 14-15 1.4450666370821779 1104 16-17 1.8573478524227198 1104 18-19 0.8689753852832247 1104 20-21 0.4460463605357319 1104 22-23 0.5476384073485043 1104 24-25 1.0772172040602541 1104 26-27 0.7145550654762793 1104 28-29 0.44446580478959774 1104 30-31 0.4469196514589555 1104 32-33 0.8233869833228944 1104 34-35 0.7362293749738811 1104 36-37 0.8962459555567825 1104 38-39 0.8900071607083966 1104 40-41 0.6795096335498556 1104 42-43 0.42615556772774 1104 44-45 0.45115669603947595 1104 46-47 0.23230384055106867 1104 48-49 0.07942049867253687 1104 50-51 0.08237912042091722 1104 52-53 0.03655791491669902 1104 54-55 0.20193157276053242 1104 56-57 0.3095494904577798 1104 58-59 0.5802199343389596 1104 60-61 0.5472338119389946 1104 62-63 0.592342383163583 1104 64-65 0.5632602994688867 1104 66-67 0.422005860462761 1104 68-69 0.5498509455610652 1104 70-71 0.6386980787698029 1104 72-73 0.7108419887839545 1104 74-75 0.5819842008737695 1104 76-77 0.22370505949697161 1104 78-79 0.23256865532586346 1104 80-81 0.6127310756206725 1104 82-83 0.5535669443502833 1104 84-85 0.7874729132864964 1104 86-87 0.6317158349350684 1104 88-89 0.4002990761230265 1104 90-91 0.2906673354539784 1104 92-93 0.31990741688175817 1104 94-95 0.06518357873114411 1104 96-97 0.33310691492178535 1104 98-99 0.5922762967187616 1104 100-101 0.6343891917104187 1104 102-103 0.1571623679842702 1104 104-105 0.3835586472697088 1104 106-107 0.53690656392709 1104 108-109 0.6446948350545902 1104 110-111 0.6322667169095197 1104 112-113 0.9087789933045762 1104 114-115 0.6166474014242667 1104 116-117 0.7331865040173717 1104 118-119 0.8657237346015911 1104 120-121 0.6214471698421953 1104 122-123 0.41867237145181235 1104 124-125 0.37024580731376133 1105 1 -0.2121335221426648 1105 2 0.3055668385959365 1105 3 0.15750005478428974 1105 4 -0.4195625235503826 1105 5 -0.04063447488760019 1105 6 -0.1752335936344025 1105 7 0.35807852750740565 1105 8 -0.5523710572510971 1105 9 -0.35435134849888783 1105 10-11 -0.08595911679372037 1105 12-13 0.24752063879257946 1105 14-15 0.30267054437963026 1105 16-17 0.1753424894654323 1105 18-19 -0.4965771908516139 1105 20-21 -0.2103125745341785 1105 22-23 -0.3970752490391618 1105 24-25 -0.05744090741264429 1105 26-27 0.18248266555289305 1105 28-29 0.28255237824295776 1105 30-31 -0.0528122006766516 1105 32-33 -0.08671644513908561 1105 34-35 -0.027944155001218718 1105 36-37 -0.18391110247807774 1105 38-39 -0.2964130653019481 1105 40-41 -0.7157786254043685 1105 42-43 -0.4192476117397952 1105 44-45 -0.4280331143416767 1105 46-47 -0.597929831159334 1105 48-49 -0.3383347206341085 1105 50-51 -0.12066627318183976 1105 52-53 -0.4087878042941817 1105 54-55 -0.022805067729798623 1105 56-57 0.36614784326375727 1105 58-59 0.23233638712578042 1105 60-61 0.211177500887473 1105 62-63 0.3680654247265025 1105 64-65 0.27838192367880765 1105 66-67 -0.13616307269180794 1105 68-69 -0.1139682461646565 1105 70-71 -0.3363928999699013 1105 72-73 -0.27627733701569923 1105 74-75 -0.30300909542962273 1105 76-77 -0.32017542316918224 1105 78-79 -0.24873760784211285 1105 80-81 -0.14020897417821843 1105 82-83 -0.16186113456179996 1105 84-85 0.039780900262172736 1105 86-87 0.02574954495230486 1105 88-89 -0.028507665880418642 1105 90-91 -0.17342958084557836 1105 92-93 0.09165612402598455 1105 94-95 0.02573711253693034 1105 96-97 0.28760796835982205 1105 98-99 0.15168062539142824 1105 100-101 0.30860868989084267 1105 102-103 0.4789781120945946 1105 104-105 0.6872456804051232 1105 106-107 0.5984273453865789 1105 108-109 0.7180907362229512 1105 110-111 0.9022341560974141 1105 112-113 0.9676182961201256 1105 114-115 0.7689458116905001 1105 116-117 0.6604418190911119 1105 118-119 0.7793609688479037 1105 120-121 1.0013648101409878 1105 122-123 0.8542594237406469 1105 124-125 0.955392330968234 1106 1 -0.5964353482343903 1106 2 -1.0789908962460402 1106 3 -0.7810452867624953 1106 4 -0.25238099285047255 1106 5 -0.7030725541096388 1106 6 -0.9822418375519995 1106 7 -0.6554115194701282 1106 8 -0.894319985176228 1106 9 -0.6906520047220397 1106 10-11 -0.42837416422823793 1106 12-13 -0.4761801673199848 1106 14-15 -0.3293966499465064 1106 16-17 -0.601203473574035 1106 18-19 -0.3574633662953417 1106 20-21 -0.3133889632961271 1106 22-23 -0.2961375262614574 1106 24-25 -2.961868128791995 1106 26-27 -0.8081492462682611 1106 28-29 -0.1305026238591509 1106 30-31 0.18942371063268482 1106 32-33 -3.209077386152117 1106 34-35 -0.9987065802850026 1106 36-37 -0.24600553280691884 1106 38-39 -0.22687900201936628 1106 40-41 -0.2575100308917797 1106 42-43 -0.42934686777649844 1106 44-45 -0.43703561859159024 1106 46-47 -0.5428089142748398 1106 48-49 -0.30282738153673705 1106 50-51 -0.3311694994600174 1106 52-53 -0.32578197323051583 1106 54-55 -0.5703126992943162 1106 56-57 -0.47720858278775324 1106 58-59 -0.6823454634600701 1106 60-61 -0.10956755915968941 1106 62-63 -0.12339819053297418 1106 64-65 -0.17209059354933487 1106 66-67 -0.23947709100756143 1106 68-69 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26-27 -1.0680536301758927 2216 28-29 -1.042490716949537 2216 30-31 -1.242210783323653 2216 32-33 -1.3440043210249328 2216 34-35 -1.0485532337217691 2216 36-37 -0.9907105661823472 2216 38-39 -1.1991232740742106 2216 40-41 -1.2965773229718849 2216 42-43 -1.4847139974896528 2216 44-45 -1.757538956134436 2216 46-47 -1.9698700724924123 2216 48-49 -1.8075360230665964 2216 50-51 -2.167620879579083 2216 52-53 -2.26561599812678 2216 54-55 -2.2176336446307765 2216 56-57 -2.32740703128135 2216 58-59 -2.084997456965393 2216 60-61 -2.435374883713177 2216 62-63 -2.4793967472712026 2216 64-65 -2.2302179614006796 2216 66-67 -2.4279941395372404 2216 68-69 -2.367540358786762 2216 70-71 -2.7873888777519333 2216 72-73 -2.726114532955176 2216 74-75 -2.6158418860827553 2216 76-77 -2.882816679633464 2216 78-79 -2.4804748229350047 2216 80-81 -2.648138489596718 2216 82-83 -2.5855634904323246 2216 84-85 -2.9212227388874155 2216 86-87 -3.068284165064931 2216 88-89 -2.8540487499639298 2216 90-91 -2.9093326645460174 2216 92-93 -3.2431360613791114 2216 94-95 -3.280468595181901 2216 96-97 -3.195153954643434 2216 98-99 -2.722941094585586 2216 100-101 -3.087349938724362 2216 102-103 -3.305881110276598 2216 104-105 -3.12948483099116 2216 106-107 -3.1543977838989967 2216 108-109 -2.9596529910323675 2216 110-111 -3.0133854570035226 2216 112-113 -3.1129601371302122 2216 114-115 -3.1377004246626896 2216 116-117 -3.2689874090261064 2216 118-119 -3.051667569746236 2216 120-121 -2.7589876127665036 2216 122-123 -2.642197193765579 2216 124-125 -2.2275802796427584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16995.0 3 3652.0 4 4913.0 5 5339.0 6 5228.0 7 3074.0 8 2198.0 9 1653.0 10 1586.0 11 1610.0 12 1433.0 13 1314.0 14 1294.0 15 1219.0 16 1177.0 17 1047.0 18 938.0 19 831.0 20 750.0 21 729.0 22 918.0 23 1944.0 24 2359.0 25 2426.0 26 3614.0 27 2156.0 28 2033.0 29 2204.0 30 2388.0 31 2889.0 32 3683.0 33 4582.0 34 6140.0 35 8661.0 36 13513.0 37 22463.0 38 8626.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.31445722725856 25.370272504302342 8.185292085044107 14.12997818339499 2 14.52023795005217 37.715625296421265 30.71601826632519 17.04811848720138 3 13.148230957898019 52.16404460885944 21.578112931419977 13.109611501822569 4 6.998299377341743 31.795545859220965 26.922008496337902 34.28414626709939 5 6.607359427615131 61.86743273732495 23.024127160502193 8.501080674557736 6 17.364527013293223 52.39643887962925 15.62800655853219 14.611027548545334 7 20.837172746490324 53.973115073987756 14.522467342403383 10.667244837118544 8 21.922597430755054 51.39167434549298 13.499376659981571 13.186351563770394 9 22.151757220968758 36.62282930530994 12.689798158424296 28.535615315297 10-11 16.28419077043993 48.98401664058106 19.10617856102337 15.625614027955633 12-13 17.70885561352395 49.088691645775455 18.61914763872891 14.583305101971678 14-15 17.74417036788595 47.340034488249266 18.315699606671345 16.600095537193443 16-17 15.494379662440968 48.89253264132151 20.01199276368835 15.601094932549172 18-19 14.997442212638518 46.51243152413637 22.361837135007132 16.128289128217986 20-21 18.55962435588621 44.04908308009202 22.14226910997957 15.249023454042208 22-23 18.537443798699318 44.24220641389927 21.364971077098353 15.855378710303064 24-25 17.00618277293131 45.2548488184975 22.520877445583128 15.218090962988057 26-27 17.206392010325867 44.58112141364112 22.80465206093888 15.407834515094128 28-29 16.64600144995291 43.77943099511488 24.106822231707895 15.467745323224316 30-31 16.34133286356984 44.54069326250745 24.012819122987697 15.105154750935009 32-33 16.53832733136618 45.28983911566124 22.885958107059736 15.285875445912847 34-35 16.334273992743483 44.20274880328474 23.910252284176256 15.552724919795516 36-37 16.25037687974064 45.364125113910845 22.888203071273463 15.497294935075054 38-39 15.979863407230743 46.159683451677594 23.065613312374655 14.794839828717004 40-41 15.400277116229594 46.499290270781174 23.913978786041202 14.186453826948028 42-43 14.953723779066616 46.75185646918532 23.117106618244893 15.177313133503171 44-45 15.728548053241548 46.67341953974314 22.67406998370502 14.923962423310286 46-47 15.21700097905367 47.14262000182937 22.427782087721855 15.212596931395101 48-49 14.739131465816586 47.59491441018744 22.33601073217994 15.329943391816037 50-51 14.921929785929406 47.54440465744978 22.40305166625449 15.130613890366314 52-53 14.148220419944305 48.18417112154535 22.066047387713343 15.601561070796999 54-55 14.567622687020213 48.44570468388994 21.90580726467061 15.08086536441924 56-57 14.819620371091832 48.31579054349336 21.043556031343268 15.821033054071542 58-59 14.370939001172141 46.984951860860605 22.786649773363234 15.857459364604013 60-61 14.525177516396553 48.18113447883354 21.609911106293023 15.68377689847688 62-63 14.456117052811306 47.602334822804835 22.19064105995264 15.750907064431216 64-65 14.355114098325112 46.634302672231556 23.199089381538297 15.811493847905037 66-67 14.36043714810323 46.82844037318843 22.028822505132357 16.78229997357598 68-69 14.842794567511442 46.385172788774575 22.221168276380734 16.550864367333247 70-71 14.562054304927418 45.81838508054271 22.6234395379169 16.996121076612972 72-73 14.88276922764214 45.616846270330335 22.173063176901422 17.327321325126107 74-75 15.138929353018096 46.16241962694708 22.278495592609406 16.42015542742542 76-77 14.807951596620436 45.374441877324806 23.96387362544294 15.853732900611817 78-79 14.491103974416303 45.028591948182175 23.699811645459842 16.780492431941678 80-81 14.37771439024886 45.78638553318925 23.382884012114395 16.4530160644475 82-83 14.810222637776604 44.683388213613014 22.866105668252096 17.640283480358278 84-85 14.279084106007378 44.34883650025238 23.34554028463313 18.02653910910711 86-87 14.932906936958606 44.64593630478307 23.286594599355663 17.13456215890266 88-89 15.569791352600216 43.37826530301131 24.28867124907262 16.763272095315852 90-91 15.370543518029189 43.57460330365733 23.92983454611976 17.12501863219372 92-93 14.65516657294045 44.87848339690907 23.051228716809064 17.41512131334142 94-95 14.33493231432172 44.49232353618711 23.608344512649566 17.564399636841607 96-97 15.354334709861883 44.457242358233934 22.827902312771226 17.360520619132956 98-99 14.298006694039053 45.41038253587544 22.614740436603114 17.67687033348239 100-101 13.974822824776076 46.40601921487323 22.84103689851891 16.778121061831783 102-103 14.27405093737508 47.12520241474867 22.149424430697934 16.451322217178323 104-105 13.53239291570118 48.144911039744166 22.0117348943724 16.31096115018226 106-107 13.228224820198722 48.959811915836404 22.005711633642402 15.806251630322473 108-109 13.044656589404646 50.172094882548635 20.569403698007356 16.213844830039363 110-111 12.846181208394736 50.34740925182513 20.6406152074123 16.165794332367835 112-113 13.221743431304795 50.26017317777145 20.853151212108894 15.664932178814858 114-115 12.850159390488065 50.79593613539891 20.314580249130213 16.039324224982806 116-117 12.600825917130498 50.24543763783635 20.950109591547054 16.203626853486096 118-119 12.87700933313007 49.719328556667854 21.03697681115233 16.36668529904975 120-121 12.943954361288398 49.747957234033905 20.651245985609172 16.656842419068525 122-123 12.872518098696759 49.09870692137526 20.978904897573404 17.049870082354577 124-125 12.866420269185298 49.64547896756971 20.53918363613574 16.948917127109254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4235.0 1 4641.0 2 5300.0 3 4509.0 4 2838.5 5 1895.5 6 1398.0 7 1215.5 8 1241.0 9 1229.0 10 1007.5 11 750.5 12 659.0 13 619.0 14 571.5 15 543.0 16 530.5 17 524.0 18 525.0 19 555.0 20 622.0 21 679.0 22 743.5 23 810.0 24 900.5 25 1014.5 26 1222.5 27 1429.0 28 1646.0 29 1927.5 30 2410.5 31 2784.5 32 3291.0 33 4295.5 34 5369.5 35 5898.0 36 6586.0 37 7419.5 38 7062.0 39 6571.0 40 5893.0 41 4845.5 42 4074.5 43 3749.5 44 3690.0 45 3399.5 46 2900.5 47 2353.5 48 2302.0 49 2265.5 50 2443.5 51 2249.5 52 1349.0 53 947.0 54 859.5 55 827.5 56 806.5 57 781.0 58 736.0 59 792.0 60 784.0 61 777.0 62 701.0 63 411.0 64 249.0 65 285.5 66 305.0 67 221.0 68 140.0 69 112.0 70 108.0 71 99.5 72 127.0 73 174.5 74 136.0 75 77.0 76 51.0 77 36.0 78 37.5 79 38.5 80 31.0 81 21.5 82 10.5 83 7.0 84 8.0 85 6.5 86 5.0 87 4.5 88 4.0 89 2.5 90 1.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 6.775343171131618E-4 5 6.775343171131618E-4 6 0.0 7 0.0013550686342263237 8 0.0013550686342263237 9 0.002032602951339485 10-11 0.002032602951339485 12-13 0.0027101372684526473 14-15 0.005081507378348713 16-17 0.003387671585565809 18-19 0.00372643874412239 20-21 0.005081507378348713 22-23 0.09011206417605051 24-25 0.004403973061235552 26-27 0.002371370109896066 28-29 0.002032602951339485 30-31 0.0013550686342263237 32-33 0.002371370109896066 34-35 0.0016938357927829045 36-37 3.387671585565809E-4 38-39 0.0013550686342263237 40-41 0.0016938357927829045 42-43 0.0013550686342263237 44-45 0.0016938357927829045 46-47 0.0016938357927829045 48-49 3.387671585565809E-4 50-51 0.0016938357927829045 52-53 0.002032602951339485 54-55 0.002032602951339485 56-57 0.0016938357927829045 58-59 6.775343171131618E-4 60-61 0.0013550686342263237 62-63 0.0016938357927829045 64-65 0.0013550686342263237 66-67 6.775343171131618E-4 68-69 3.387671585565809E-4 70-71 0.0010163014756697426 72-73 3.387671585565809E-4 74-75 6.775343171131618E-4 76-77 6.775343171131618E-4 78-79 0.0 80-81 6.775343171131618E-4 82-83 0.0 84-85 3.387671585565809E-4 86-87 3.387671585565809E-4 88-89 3.387671585565809E-4 90-91 0.0 92-93 6.775343171131618E-4 94-95 0.0 96-97 3.387671585565809E-4 98-99 0.0 100-101 0.0 102-103 6.775343171131618E-4 104-105 0.0 106-107 3.387671585565809E-4 108-109 6.775343171131618E-4 110-111 0.0010163014756697426 112-113 0.0 114-115 3.387671585565809E-4 116-117 3.387671585565809E-4 118-119 0.0010163014756697426 120-121 0.0 122-123 3.387671585565809E-4 124-125 3.387671585565809E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 147594.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.14390828895484 #Duplication Level Percentage of deduplicated Percentage of total 1 79.07004219409282 31.741805222434515 2 7.139240506329115 5.731940322777349 3 3.7569620253164557 4.524574169681694 4 2.4556962025316453 3.9432497255986014 5 1.6472573839662448 3.306367467512229 6 1.1021097046413502 2.654579454449368 7 0.7864978902953587 2.210116942423134 8 0.540084388185654 1.7344878518096942 9 0.4472573839662447 1.6159193463148906 >10 2.717299578059072 20.4879602151849 >50 0.21940928270042193 6.149301462119056 >100 0.1029535864978903 6.618832743878478 >500 0.008438818565400843 2.1870807756412862 >1k 0.005063291139240506 3.507595159694839 >5k 0.0016877637130801688 3.586189140479965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5293 3.586189140479965 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1907 1.2920579427347996 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1778 1.2046560158272017 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1492 1.0108812011328374 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 966 0.6544981503313143 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 634 0.42955675704974455 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 562 0.38077428621759696 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 549 0.3719663400951258 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 517 0.35028524194750466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.32318386926297815 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 464 0.31437592314050705 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 362 0.24526742279496455 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.19851755491415637 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 290 0.19648495196281693 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 266 0.18022412835210103 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 265 0.17954659403498788 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 248 0.1680285106440641 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 248 0.1680285106440641 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.16193070179004568 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.15718796157025353 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 231 0.15651042725314038 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 205 0.13889453500819815 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 181 0.12263371139748228 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 171 0.11585836822635066 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 166 0.11247069664078485 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 152 0.1029852162012006 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.10027507893274794 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0020326029513394856 0.0 24-25 0.0 0.0 0.0 0.0071141103296881985 0.0 26-27 0.0 0.0 0.0 0.008807946122471103 0.0 28-29 0.0 0.0 0.0 0.011856850549480331 0.0 30-31 0.0 0.0 0.0 0.03421548301421467 0.0 32-33 0.0 0.0 0.0 0.06809219886987276 0.0 34-35 0.0 0.0 0.0 0.10535658631109665 0.0 36-37 0.0 0.0 0.0 0.15142891987479165 0.0 38-39 0.0 0.0 0.0 0.20800303535374068 0.0 40-41 0.0 0.0 0.0 0.26728728810114233 0.0 42-43 0.0 0.0 0.0 0.33063674675122295 0.0 44-45 0.0 0.0 0.0 0.4129571662804721 0.0 46-47 0.0 0.0 0.0 0.49222868138271203 0.0 48-49 0.0 0.0 0.0 0.5806469097659797 0.0 50-51 0.0 0.0 0.0 0.6721140425762565 0.0 52-53 0.0 0.0 0.0 0.7649362440207597 0.0 54-55 0.0 0.0 0.0 0.884182283832676 0.0 56-57 0.0 0.0 0.0 1.0020732550103664 0.0 58-59 0.0 0.0 0.0 1.1589224494220631 0.0 60-61 0.0 0.0 0.0 1.3039147932842798 0.0 62-63 0.0 0.0 0.0 1.449245904305053 0.0 64-65 0.0 0.0 0.0 1.6159193463148909 0.0 66-67 0.0 0.0 0.0 1.8157919698632736 0.0 68-69 0.0 0.0 0.0 2.060381858341125 0.0 70-71 0.0 0.0 0.0 2.294808732062279 0.0 72-73 0.0 0.0 0.0 2.5092483434285944 0.0 74-75 0.0 0.0 0.0 2.736899873978617 0.0 76-77 0.0 0.0 0.0 3.00113825765275 0.0 78-79 0.0 0.0 0.0 3.337534046099435 0.0 80-81 0.0 0.0 0.0 3.6834153149857043 0.0 82-83 0.0 0.0 0.0 3.978481510088486 0.0 84-85 0.0 0.0 0.0 4.294212501863219 0.0 86-87 0.0 0.0 0.0 4.657032128677318 0.0 88-89 0.0 0.0 0.0 5.0649077875794415 0.0 90-91 0.0 0.0 0.0 5.480913858286923 0.0 92-93 0.0 0.0 0.0 5.889128284347603 0.0 94-95 0.0 0.0 0.0 6.348835318508883 0.0 96-97 0.0 0.0 0.0 6.833949889561906 0.0 98-99 0.0 0.0 0.0 7.372589671666869 0.0 100-101 0.0 0.0 0.0 7.9739013781048005 0.0 102-103 0.0 0.0 0.0 8.611461170508285 0.0 104-105 0.0 0.0 0.0 9.168733146333862 0.0 106-107 0.0 0.0 0.0 9.760898139490767 0.0 108-109 0.0 0.0 0.0 10.382535875442091 0.0 110-111 0.0 0.0 0.0 11.141374310608832 0.0 112-113 0.0 0.0 0.0 11.88462945648197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAAGG 95 0.0 100.19055 2 TACAAAG 70 1.8189894E-12 76.48475 2 GAACAAA 890 0.0 74.86147 1 CGCAAAG 40 1.9755662E-6 74.36017 2 GGGTATG 25 0.0020521474 71.40997 7 GGAACGC 25 0.0020521474 71.40997 8 GACTGCG 25 0.0020521474 71.40997 7 GAAAACA 555 0.0 67.52708 1 GTACATA 115 0.0 62.07458 1 CCTGCAT 15 0.0042310953 59.50831 34-35 GGGATAC 30 0.0042269067 59.50831 9 CCAGGAG 30 0.0042325864 59.48814 3 CGACATG 15 0.0042367787 59.48814 80-81 CGCTGAT 15 0.0042367787 59.48814 62-63 GTACTAT 30 0.0042325864 59.48814 1 GCTCCGT 15 0.0042367787 59.48814 116-117 GTCGGGA 50 7.4287364E-6 59.48814 2 GGGAGTG 40 1.7686411E-4 59.488136 5 GGATATG 105 1.8189894E-12 56.65537 1 GTACAAG 245 0.0 55.846012 1 >>END_MODULE