##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147594 Sequences flagged as poor quality 0 Sequence length 125 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.403864655744812 31.0 31.0 34.0 30.0 34.0 2 31.060483488488693 31.0 31.0 34.0 28.0 34.0 3 31.295283006084258 31.0 31.0 34.0 28.0 34.0 4 35.295127173191325 35.0 35.0 37.0 33.0 37.0 5 35.067780533084 37.0 35.0 37.0 33.0 37.0 6 35.37861972708918 37.0 35.0 37.0 33.0 37.0 7 35.20210171145168 37.0 35.0 37.0 32.0 37.0 8 35.344255186525196 37.0 35.0 37.0 32.0 37.0 9 36.82912584522406 39.0 37.0 39.0 32.0 39.0 10-11 36.79285065788582 39.0 37.0 39.0 32.0 39.0 12-13 36.71305066601623 39.0 37.0 39.0 32.0 39.0 14-15 37.35764665230293 39.0 36.0 41.0 32.0 41.0 16-17 37.37419881567001 39.0 36.5 41.0 32.0 41.0 18-19 37.92780194316842 40.0 37.0 41.0 32.5 41.0 20-21 38.05451102348334 40.0 37.0 41.0 33.0 41.0 22-23 37.74153420870767 40.0 36.5 41.0 32.0 41.0 24-25 37.56835304958196 40.0 36.0 41.0 32.0 41.0 26-27 37.17137891784219 40.0 35.0 41.0 32.0 41.0 28-29 36.845583153786734 39.0 35.0 41.0 31.5 41.0 30-31 35.98916283859778 37.5 35.0 40.0 30.5 41.0 32-33 34.962329091968506 36.0 34.5 40.0 29.5 41.0 34-35 34.26274103283332 35.0 33.5 40.0 26.0 41.0 36-37 33.65934252069867 35.0 33.0 40.0 22.0 41.0 38-39 33.38458202907977 35.0 33.0 40.0 21.0 41.0 40-41 33.05118094231473 35.0 33.0 40.0 17.0 41.0 42-43 32.58504071981246 35.0 33.0 40.0 12.0 41.0 44-45 31.75475290323455 35.0 32.0 40.0 3.5 41.0 46-47 30.411371735978427 35.0 27.5 40.0 2.0 41.0 48-49 29.472180440939333 35.0 23.5 40.0 2.0 41.0 50-51 29.434285946583195 35.0 24.5 40.0 2.0 41.0 52-53 29.559494288385707 35.0 27.0 40.0 2.0 41.0 54-55 29.525004403973064 35.0 27.0 40.0 2.0 41.0 56-57 29.38964321042861 35.0 27.0 40.0 2.0 41.0 58-59 29.243471956854613 35.0 26.0 40.0 2.0 41.0 60-61 29.043199588059135 35.0 26.0 39.0 2.0 41.0 62-63 28.858131089339675 35.0 26.0 39.0 2.0 41.0 64-65 28.642343184682304 35.0 26.0 39.0 2.0 41.0 66-67 28.391174437985285 35.0 25.5 38.5 2.0 41.0 68-69 28.131028361586516 35.0 25.0 38.0 2.0 40.0 70-71 27.838414840711682 35.0 24.0 37.0 2.0 40.0 72-73 27.476669105790208 35.0 23.0 37.0 2.0 39.5 74-75 27.13973128988983 35.0 20.5 36.5 2.0 39.0 76-77 26.753983901784625 34.5 19.5 36.0 2.0 39.0 78-79 26.34670786075315 34.0 15.5 36.0 2.0 38.5 80-81 25.997903031288537 34.0 8.5 35.0 2.0 37.0 82-83 25.619689824789624 34.0 3.5 35.0 2.0 37.0 84-85 25.215516890930527 34.0 2.0 35.0 2.0 36.5 86-87 24.875367562367032 34.0 2.0 35.0 2.0 36.0 88-89 24.52631204520509 33.0 2.0 35.0 2.0 36.0 90-91 24.202606474517935 33.0 2.0 35.0 2.0 36.0 92-93 23.902350366546067 33.0 2.0 35.0 2.0 35.0 94-95 23.63302370015041 33.0 2.0 35.0 2.0 35.0 96-97 23.38906730625906 33.0 2.0 35.0 2.0 35.0 98-99 23.13028307383769 33.0 2.0 35.0 2.0 35.0 100-101 22.864869168123366 33.0 2.0 35.0 2.0 35.0 102-103 21.858690055151293 30.5 2.0 34.0 2.0 35.0 104-105 22.268469585484503 32.0 2.0 35.0 2.0 35.0 106-107 22.17648752659322 32.0 2.0 35.0 2.0 35.0 108-109 21.972332886160686 32.0 2.0 35.0 2.0 35.0 110-111 21.770156645934115 32.0 2.0 35.0 2.0 35.0 112-113 21.505759041695462 32.0 2.0 35.0 2.0 35.0 114-115 21.27808718511593 32.0 2.0 35.0 2.0 35.0 116-117 21.01185685054948 32.0 2.0 35.0 2.0 35.0 118-119 20.766657181186226 31.0 2.0 35.0 2.0 35.0 120-121 20.450523056492813 31.0 2.0 35.0 2.0 35.0 122-123 20.16053498109679 31.0 2.0 35.0 2.0 35.0 124-125 18.774940038212936 27.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08728043946091546 1101 2 0.5460102333344601 1101 3 0.393274741553725 1101 4 0.2928700677282947 1101 5 0.8664113979786876 1101 6 0.5154903266815509 1101 7 0.39744538505859595 1101 8 0.503784063160154 1101 9 0.0074231323091282775 1101 10-11 0.4820812379675843 1101 12-13 0.45164894883701834 1101 14-15 0.5273251493427935 1101 16-17 0.4267127352817184 1101 18-19 0.5516785338721135 1101 20-21 0.37373886516505195 1101 22-23 0.2910169968145553 1101 24-25 0.35206577093948255 1101 26-27 0.7174459895247409 1101 28-29 0.5927725668875183 1101 30-31 0.022163571820740913 1101 32-33 -0.5217072889543957 1101 34-35 -0.6280455194851697 1101 36-37 -0.2889466293855065 1101 38-39 -0.4481886096972332 1101 40-41 -0.6254896200537416 1101 42-43 0.03521214462251976 1101 44-45 -0.12471330413304571 1101 46-47 -0.31071598366295206 1101 48-49 -0.2893173598445671 1101 50-51 -1.0154588315683455 1101 52-53 -0.9264927914568126 1101 54-55 -0.5902154839753386 1101 56-57 -0.3799815413595553 1101 58-59 -0.21916746827274025 1101 60-61 -0.3257801721743121 1101 62-63 -0.6523145611217096 1101 64-65 -1.1169115577425046 1101 66-67 -0.9683533343819519 1101 68-69 -1.0215907304228118 1101 70-71 -1.2604814396360773 1101 72-73 -0.767255880088765 1101 74-75 -0.636795633061304 1101 76-77 -0.7716769643796617 1101 78-79 -0.6079705661699961 1101 80-81 -0.825534457194447 1101 82-83 -0.6002560075987375 1101 84-85 -0.7111259895081687 1101 86-87 -0.5348600002091288 1101 88-89 -0.341738828722832 1101 90-91 -0.49820189960179917 1101 92-93 -0.6067324349423338 1101 94-95 -0.7302692565827797 1101 96-97 -0.7145282036593521 1101 98-99 -0.519867800216435 1101 100-101 -0.5300417577427368 1101 102-103 -0.537013992823475 1101 104-105 -0.22085541970259825 1101 106-107 0.04500539061392672 1101 108-109 -0.26405688945518335 1101 110-111 0.0969478556045047 1101 112-113 0.3311114375942026 1101 114-115 0.2808351818048287 1101 116-117 -0.1226852882359708 1101 118-119 -0.18174067499884572 1101 120-121 -0.5766947012613031 1101 122-123 -0.6181244460156705 1101 124-125 -0.4069072988085942 1102 1 0.43881042873521636 1102 2 0.7774542279363494 1102 3 0.3711199957147677 1102 4 0.30850209651821814 1102 5 0.522056922045266 1102 6 0.44756410086058906 1102 7 0.675560544752706 1102 8 0.4816293173212003 1102 9 0.6923084224575788 1102 10-11 0.7035162357183751 1102 12-13 0.5616916838797579 1102 14-15 0.9598487660769379 1102 16-17 0.9465620380257533 1102 18-19 0.4335948631568627 1102 20-21 0.1790132691236721 1102 22-23 0.488048035950861 1102 24-25 0.5875021182179339 1102 26-27 0.5266565158405214 1102 28-29 0.6287599712959704 1102 30-31 0.17904616291386333 1102 32-33 0.5752900119902691 1102 34-35 0.5862482277033152 1102 36-37 1.4322072167683402 1102 38-39 1.504971534252384 1102 40-41 1.085317847332668 1102 42-43 1.4544654059810398 1102 44-45 2.055673560464342 1102 46-47 2.0927365579474824 1102 48-49 2.579609765661502 1102 50-51 2.360497083861258 1102 52-53 2.2574365832620344 1102 54-55 2.151237507477653 1102 56-57 2.1575240816723813 1102 58-59 1.8947551588077864 1102 60-61 2.2586777675467857 1102 62-63 2.218243243646622 1102 64-65 2.348169413917411 1102 66-67 2.092150489279767 1102 68-69 2.1492369798785838 1102 70-71 1.9066350695856933 1102 72-73 1.7646267110043539 1102 74-75 1.7980334267677591 1102 76-77 2.0118925362951003 1102 78-79 1.984640769862395 1102 80-81 2.1419644181991657 1102 82-83 2.086203731492578 1102 84-85 2.037355791868343 1102 86-87 1.9556370758142627 1102 88-89 2.116650734929891 1102 90-91 1.9625493374652265 1102 92-93 2.1695833545313477 1102 94-95 2.267762682238633 1102 96-97 2.3204470550001197 1102 98-99 1.977995447646812 1102 100-101 1.8522681837777348 1102 102-103 1.606035939700142 1102 104-105 1.8746798929379764 1102 106-107 1.5865033663224288 1102 108-109 1.7032170646608762 1102 110-111 1.8178205501614109 1102 112-113 1.6567974474907388 1102 114-115 1.559681335650982 1102 116-117 1.305002521030783 1102 118-119 1.0222629237415966 1102 120-121 1.5575270711183649 1102 122-123 1.5334162647355676 1102 124-125 1.5442847839624356 1103 1 0.05801870583822932 1103 2 0.47763416495839195 1103 3 0.09412944240842336 1103 4 0.16039143524965738 1103 5 0.40914644071372663 1103 6 -0.07852676733554631 1103 7 0.30770179531500474 1103 8 0.2345532939293875 1103 9 -0.005397380511382721 1103 10-11 0.2192607251470733 1103 12-13 0.1396831368712128 1103 14-15 0.0038208758385209762 1103 16-17 0.30064435921334365 1103 18-19 0.26535374754733 1103 20-21 0.3010892925154849 1103 22-23 0.5346067404042998 1103 24-25 0.298646967520682 1103 26-27 0.29223231431105745 1103 28-29 0.45815718227213154 1103 30-31 0.11404391370108158 1103 32-33 -0.11999788724499183 1103 34-35 0.1775100360703874 1103 36-37 -0.0667244071632851 1103 38-39 0.028307116798494292 1103 40-41 -0.33702808159220865 1103 42-43 -0.03316392375355548 1103 44-45 -0.43667911609885834 1103 46-47 -0.7423399152868804 1103 48-49 -0.6504284709556813 1103 50-51 -1.0090485751580864 1103 52-53 -1.1636722786363016 1103 54-55 -1.780386424146279 1103 56-57 -1.493229404607419 1103 58-59 -1.4178854169906892 1103 60-61 -1.8065494029435456 1103 62-63 -1.5988957577029055 1103 64-65 -2.157509848340794 1103 66-67 -2.1221994882281052 1103 68-69 -2.023727482559565 1103 70-71 -1.9314216105762476 1103 72-73 -1.6924695553024414 1103 74-75 -1.3226930689587384 1103 76-77 -1.192617135319832 1103 78-79 -1.1913038995033247 1103 80-81 -1.310577192237183 1103 82-83 -1.6066662640089966 1103 84-85 -1.606425134807317 1103 86-87 -1.319048034397163 1103 88-89 -1.58319182017582 1103 90-91 -1.1926463440462456 1103 92-93 -1.209296537506436 1103 94-95 -1.102064128377652 1103 96-97 -0.9880324771636246 1103 98-99 -0.4792695096181454 1103 100-101 -0.6155118432128219 1103 102-103 -0.6972704030798837 1103 104-105 -1.2080349068820873 1103 106-107 -1.3267894811809455 1103 108-109 -1.2790141544124474 1103 110-111 -1.8688641102074612 1103 112-113 -2.1817090752263084 1103 114-115 -1.8473699464003026 1103 116-117 -1.7402066557573406 1103 118-119 -1.6390056322637996 1103 120-121 -1.491224615791218 1103 122-123 -1.2805176084088323 1103 124-125 -1.2274201193214154 1104 1 0.4661941053179781 1104 2 0.49606597469454883 1104 3 0.6525463877818929 1104 4 0.2510268867546728 1104 5 0.5301423530139928 1104 6 0.40538256005203976 1104 7 0.6638519253781823 1104 8 0.4137803470694834 1104 9 0.7875792081173785 1104 10-11 0.6211745118434635 1104 12-13 0.5113293650391739 1104 14-15 0.6327320612714473 1104 16-17 0.9412798107183562 1104 18-19 0.5040754123559523 1104 20-21 0.48345644227393336 1104 22-23 0.5487650458669506 1104 24-25 0.2359193569669884 1104 26-27 -0.11226415540279788 1104 28-29 0.20930917038063512 1104 30-31 -0.5227082230510547 1104 32-33 -0.2576047248518307 1104 34-35 -0.11033834743016513 1104 36-37 -0.38627104410122826 1104 38-39 -0.7074045145653045 1104 40-41 -0.47457917783896164 1104 42-43 -0.6717741058419975 1104 44-45 -0.3801387965150589 1104 46-47 -0.3440864779030086 1104 48-49 -0.2882582740028745 1104 50-51 0.06415841109237519 1104 52-53 0.019698215603760616 1104 54-55 0.3121070726950421 1104 56-57 0.3853844935716957 1104 58-59 0.46771473165294 1104 60-61 0.4713398575544119 1104 62-63 0.5889526257976527 1104 64-65 0.7085622438182497 1104 66-67 0.6245303519799954 1104 68-69 0.6050722201531791 1104 70-71 0.7173520795017865 1104 72-73 0.7516404409814719 1104 74-75 0.6157870499561611 1104 76-77 -0.006013642194602653 1104 78-79 -0.014157857139892371 1104 80-81 0.19936335031205488 1104 82-83 0.3780420228732062 1104 84-85 0.2545373326768896 1104 86-87 0.12625111090197905 1104 88-89 0.08873311479259272 1104 90-91 0.3215119614164088 1104 92-93 0.11210814476780939 1104 94-95 -0.03881626512978542 1104 96-97 0.17018008322284928 1104 98-99 0.4700244331540553 1104 100-101 0.5153688702765855 1104 102-103 0.7104219046124243 1104 104-105 0.8777882072018954 1104 106-107 0.8948939078937492 1104 108-109 0.5925763323954314 1104 110-111 0.6646550276595065 1104 112-113 0.7202604528301748 1104 114-115 0.8629422052161999 1104 116-117 0.67155477122148 1104 118-119 0.7219196743136784 1104 120-121 0.6967909917896087 1104 122-123 0.9602850671764536 1104 124-125 1.0202573240082025 1105 1 -0.09929143385076244 1105 2 0.054368981781315995 1105 3 0.07876741823582734 1105 4 -0.13374614523153383 1105 5 0.26906210018886156 1105 6 0.301532722856102 1105 7 0.2123292285767988 1105 8 0.31335897185400796 1105 9 0.29382322267397853 1105 10-11 0.1725439812937637 1105 12-13 0.043152770517053796 1105 14-15 0.333718462344045 1105 16-17 0.3424098624325822 1105 18-19 0.2569196950604109 1105 20-21 0.1338107805233122 1105 22-23 0.13900637894490586 1105 24-25 0.09491188713362675 1105 26-27 0.3294136840915556 1105 28-29 0.3952218307905184 1105 30-31 -0.12402980895414117 1105 32-33 -0.29901441648178917 1105 34-35 -0.2469788377576947 1105 36-37 -0.6068896995312159 1105 38-39 -0.13193046744789427 1105 40-41 -0.33918365973897835 1105 42-43 -0.09832074441698069 1105 44-45 -0.14754925428177046 1105 46-47 -0.2157011111150382 1105 48-49 0.20760458751790623 1105 50-51 -0.3803445576875504 1105 52-53 -0.4437091776159292 1105 54-55 -0.6053704220377654 1105 56-57 -0.8400704001268302 1105 58-59 -0.8206208362459293 1105 60-61 -0.7003801194614852 1105 62-63 -0.5433496151523585 1105 64-65 -0.5634833413186939 1105 66-67 -0.12138996979744121 1105 68-69 -0.32067955153806693 1105 70-71 -0.1780894068035188 1105 72-73 -0.10214235924837212 1105 74-75 -0.24400034709421092 1105 76-77 -0.23123869486650506 1105 78-79 -0.6855713794536271 1105 80-81 -0.630506406439526 1105 82-83 -0.8402782222905536 1105 84-85 -0.43720754378355764 1105 86-87 -0.2673612238997798 1105 88-89 -0.34277425927367844 1105 90-91 -0.24353304911797125 1105 92-93 -0.31671775065452223 1105 94-95 -0.44145943693595413 1105 96-97 -0.29505646150038345 1105 98-99 -0.5190676947907811 1105 100-101 -0.5497431772238919 1105 102-103 -0.31028294120255495 1105 104-105 -0.19737938521334897 1105 106-107 -0.2294213573811028 1105 108-109 -0.35526890797997623 1105 110-111 -0.3028224670733053 1105 112-113 -0.2409921807649198 1105 114-115 -0.12809399474549465 1105 116-117 0.19859939868747745 1105 118-119 0.01913285186587288 1105 120-121 0.08168852099857205 1105 122-123 -0.3116934809943963 1105 124-125 -0.33120791253652015 1106 1 0.35556809052454597 1106 2 0.5900042752538148 1106 3 0.2621260377909138 1106 4 0.30881147993526525 1106 5 0.4871511929923429 1106 6 0.32697736430966273 1106 7 0.4307290677198736 1106 8 0.38024255165183973 1106 9 0.5384902671107028 1106 10-11 0.4728072017225955 1106 12-13 0.4505229425242874 1106 14-15 0.18063933170262914 1106 16-17 0.385786395600185 1106 18-19 0.2214306351594857 1106 20-21 0.36114000713051553 1106 22-23 0.18730915555484984 1106 24-25 -0.08206729286163039 1106 26-27 0.348497165181719 1106 28-29 0.5506315589788713 1106 30-31 -0.29656714421287944 1106 32-33 -0.18789518128336624 1106 34-35 -0.5741124769629167 1106 36-37 -0.043415189870664506 1106 38-39 -0.22947739928476807 1106 40-41 -0.5030475162838428 1106 42-43 -0.017435300141116272 1106 44-45 -0.40403379922117466 1106 46-47 -0.6076801997143022 1106 48-49 -0.7469990281071475 1106 50-51 -0.33774489036892774 1106 52-53 -0.3044673986845332 1106 54-55 -0.3663973472941322 1106 56-57 -0.2598978445123734 1106 58-59 -0.22734209677931716 1106 60-61 0.15983886531194358 1106 62-63 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-3.0877147877584257 2216 108-109 -2.992206276300223 2216 110-111 -2.987518885383974 2216 112-113 -3.134087373256783 2216 114-115 -3.2261882295664073 2216 116-117 -3.5240974026915666 2216 118-119 -3.519384673512409 2216 120-121 -3.3879916169060422 2216 122-123 -3.5984105849974632 2216 124-125 -3.2319165890352775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 22.0 4 55.0 5 149.0 6 387.0 7 700.0 8 1007.0 9 1644.0 10 2497.0 11 3139.0 12 3227.0 13 4184.0 14 6024.0 15 4290.0 16 1822.0 17 1446.0 18 1571.0 19 1552.0 20 1812.0 21 1967.0 22 2168.0 23 2266.0 24 2592.0 25 2713.0 26 2886.0 27 3054.0 28 3278.0 29 3603.0 30 4101.0 31 4663.0 32 5435.0 33 6309.0 34 7889.0 35 10830.0 36 13619.0 37 23942.0 38 10700.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.95766072990326 16.582485304985116 15.54993593176903 12.9099180333426 2 19.469758050856072 26.765497008665722 36.79917069237701 16.9655742481012 3 16.612463921297614 33.824545713240376 37.90872257679852 11.654267788663496 4 8.748323102565145 21.423635107118173 35.906608669729124 33.92143312058756 5 5.829277221145111 44.61068102010657 36.15250077958702 13.4075409791613 6 19.418811062780332 42.27610878490996 18.140303806387795 20.16477634592192 7 17.096900958033526 40.46031681504668 25.587083485778557 16.85569874114124 8 28.583140236052955 31.642207677818877 21.5537216960039 18.22093039012426 9 25.19411358185292 10.217217502066479 23.37222380313563 41.216445112944974 10-11 22.5703619388322 27.88155345068228 29.10179275580308 20.44629185468244 12-13 27.219941190021274 30.551038660108134 30.86541458324864 11.363605566621949 14-15 22.257341084325923 27.61392739542258 37.654308440722524 12.474423079528977 16-17 12.466970202040734 27.76027480791902 40.49487106521945 19.27788392482079 18-19 11.611583126685368 19.77045137336206 53.284008835047494 15.333956664905077 20-21 12.689540225212406 18.6013659091833 57.142566771006955 11.56652709459734 22-23 12.552000758838435 18.671490711004513 56.62933452579373 12.147174004363322 24-25 11.654267788663496 19.763337263032373 57.2248871905362 11.35750775776793 26-27 12.027927964551404 19.183029120424948 57.18389636435085 11.605146550672792 28-29 12.006246866403783 17.784259522744826 57.89158095857555 12.317912652275838 30-31 12.149545374473217 18.808013875902816 57.44034310337819 11.602097646245783 32-33 12.196295242354026 19.181335284632166 56.58122958927869 12.041139883735111 34-35 12.022887186766356 18.820612018821965 56.62817129480634 12.528329499605334 36-37 12.714270227787036 19.06886458799138 55.08896025583696 13.127904928384623 38-39 12.625174465086655 19.345976123690665 54.58216458663632 13.446684824586367 40-41 12.260322235321219 19.60919820588913 54.90399338726506 13.226486171524588 42-43 12.41920184294329 20.28660478352192 52.8565620977031 14.437631275831697 44-45 13.180826410440579 19.66282237189825 51.95388201493447 15.202469202726693 46-47 13.483378281315645 20.071758663215384 49.840084565450134 16.604778490018838 48-49 13.896963660141349 21.73988169048442 46.99821790363127 17.36493674574296 50-51 14.1907068927549 21.767147188860466 46.5492182338692 17.49292768451544 52-53 13.64036751951431 21.96986123156982 46.03581689071986 18.35395435819601 54-55 14.07038751075666 22.602535522384017 45.72172946748609 17.605347499373234 56-57 14.93817122336281 21.8775620828675 44.903953653826605 18.28031303994308 58-59 14.663066566839559 20.488046155357633 46.43349583470257 18.41539144310024 60-61 15.674111816139927 21.96889366185493 45.08420257401391 17.27279194799123 62-63 16.027968995609516 21.571291126890348 45.92830234701068 16.472437530489458 64-65 16.104422296450373 21.23711829151789 46.64414979030171 16.01430962173003 66-67 16.584795717867067 21.29920726336473 45.70092824717122 16.415068771596992 68-69 16.61376163261434 21.574751933546306 45.73772879874519 16.073757635094164 70-71 16.061549510971386 21.11919289104503 46.172296622026785 16.646960975956798 72-73 15.82966325631818 21.383901348329832 45.41025814757098 17.376177247781015 74-75 14.82658391658141 22.0122228018944 45.654942985100924 17.50625029642327 76-77 14.32002655934523 22.087618737889077 47.062888735314445 16.52946596745125 78-79 14.032413241730692 21.797295283006086 46.642817458704286 17.527474016558937 80-81 13.654687859940108 22.6577638657398 45.80978901581365 17.877759258506444 82-83 13.936880903017737 22.045950377386614 45.20576717210727 18.81140154748838 84-85 13.478574598474866 22.274693668759127 44.99385135524261 19.2528803775234 86-87 13.97922679783731 22.56968440451509 44.668821225795085 18.782267571852515 88-89 14.16811209279636 21.662081955661552 45.24770990297577 18.922096048566317 90-91 14.192986675971692 22.158946544662427 44.81555384662292 18.83251293274296 92-93 13.636055666219493 22.986029242381125 43.86763689580877 19.510278195590608 94-95 13.51326630169657 22.789175565071275 43.93158165754241 19.76597647568974 96-97 14.787725621984931 22.51883570925253 42.96269445498401 19.730744213778525 98-99 14.381153066962984 22.560285658145254 42.48724498438231 20.571316290509458 100-101 14.0410251198401 22.971356945644256 42.28568524823416 20.701932686281484 102-103 14.76372284311766 22.27025269070373 41.83303239007666 21.132992076101946 104-105 14.69998373895604 22.28609951758903 41.46633963900482 21.547577104450106 106-107 14.32002655934523 22.66047400300825 41.23575484098269 21.78374459666382 108-109 14.35371038501279 23.058759760827954 39.99424089977472 22.593288954384537 110-111 14.236447527999296 23.16268386712107 39.813541292608726 22.787327312270907 112-113 14.344085948643585 22.8007169163112 39.793598574298755 23.06159856074646 114-115 13.892851455155986 23.350602968931597 39.30327355035019 23.453272025562224 116-117 13.588157163574266 23.04494260195438 39.66089749671334 23.70600273775802 118-119 13.517643272179026 22.822923548643935 39.73302163270146 23.92641154647558 120-121 13.593091485642269 23.601493366579483 38.287506945103196 24.517908202675052 122-123 13.059121261090953 23.404918471540416 38.3368397516033 25.199120515765333 124-125 12.834168436815022 23.42490674960108 37.95604611532898 25.78487869825492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 937.0 1 616.0 2 915.5 3 1522.0 4 1131.0 5 962.5 6 1427.0 7 3533.5 8 5380.5 9 5709.5 10 4427.5 11 2350.0 12 1672.0 13 1359.0 14 1181.5 15 1039.0 16 968.5 17 924.0 18 908.5 19 872.0 20 848.5 21 863.0 22 878.5 23 1009.5 24 1105.5 25 1109.5 26 1236.0 27 1439.0 28 1508.5 29 1669.5 30 2104.0 31 2436.5 32 3012.5 33 3727.0 34 4410.0 35 4974.5 36 5612.5 37 6566.5 38 6612.5 39 6283.0 40 5698.0 41 4893.5 42 4314.0 43 3984.0 44 3978.0 45 3733.5 46 3198.5 47 2823.5 48 2784.0 49 2514.0 50 2781.0 51 2391.5 52 1232.0 53 879.0 54 708.0 55 656.5 56 681.0 57 638.0 58 566.5 59 589.0 60 647.5 61 650.0 62 539.5 63 323.5 64 197.5 65 224.5 66 237.5 67 165.5 68 98.5 69 83.0 70 81.5 71 67.0 72 71.5 73 116.0 74 100.0 75 43.5 76 31.5 77 36.5 78 28.0 79 12.0 80 9.5 81 4.0 82 3.5 83 3.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06436576012575038 2 6.775343171131618E-4 3 0.0 4 0.0 5 0.054202745369052945 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.387671585565809E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0027101372684526473 44-45 0.01084054907381059 46-47 0.01084054907381059 48-49 0.010163014756697427 50-51 0.00779164464680136 52-53 0.006775343171131618 54-55 0.00745287748824478 56-57 0.006097808854018456 58-59 0.00372643874412239 60-61 0.002371370109896066 62-63 0.0013550686342263237 64-65 6.775343171131618E-4 66-67 0.0027101372684526473 68-69 0.0016938357927829045 70-71 0.00372643874412239 72-73 0.0027101372684526473 74-75 6.775343171131618E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 3.387671585565809E-4 86-87 0.0 88-89 0.0013550686342263237 90-91 0.002371370109896066 92-93 0.0 94-95 0.0013550686342263237 96-97 0.0013550686342263237 98-99 0.003387671585565809 100-101 0.0010163014756697426 102-103 0.0016938357927829045 104-105 0.0013550686342263237 106-107 0.0 108-109 0.0010163014756697426 110-111 6.775343171131618E-4 112-113 0.011856850549480331 114-115 0.022019865306177756 116-117 0.018970960879168528 118-119 0.011179316232367169 120-121 0.005420274536905295 122-123 0.005081507378348713 124-125 0.005081507378348713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 147594.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.265295337208826 #Duplication Level Percentage of deduplicated Percentage of total 1 67.88164886756437 17.829315554832853 2 10.377650518495589 5.451441115492499 3 5.432595573440644 4.280661815520956 4 3.9003250270855903 4.097727549900402 5 2.7369344270752722 3.594319552285323 6 1.7257390496827114 2.719622748892231 7 1.2614146416963319 2.3191999674783528 8 0.9260692359283909 1.9458785587490008 9 0.6887478718464634 1.6281149640229278 >10 4.529742557911573 22.54766453920891 >50 0.34050456585667854 6.272612707833653 >100 0.17283186297270803 7.132403756250254 >500 0.005159160088737554 1.0921853191864168 >1k 0.012897900221843882 5.804436494708457 >5k 0.007738740133106331 13.284415355637764 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7088 4.802363239698091 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6708 4.544900199195089 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5811 3.937151916744583 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 1.7385530577123731 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1913 1.2961231486374785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 1.0515332601596272 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1435 0.972261745057387 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1101 0.7459652831415912 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 907 0.6145236256216378 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 705 0.477661693564779 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 494 0.3347019526539019 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 454 0.30760057996937545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 352 0.23849207962383295 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.1897096087916853 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 241 0.16328577042427198 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 240 0.16260823610715883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 235 0.159220564521593 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 229 0.15515535861891405 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.15380028998468773 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 213 0.14431480954510348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 209 0.1416046722766508 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 200 0.13550686342263238 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 199 0.13482932910551917 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 194 0.1314416575199534 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 188 0.12737645161727443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 180 0.12195617708036911 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 176 0.11924603981191648 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 175 0.1185685054948033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 175 0.1185685054948033 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 172 0.11653590254346383 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 171 0.11585836822635066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 164 0.11111562800655854 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 160 0.10840549073810589 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 150 0.10163014756697426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0020326029513394856 0.0 24-25 0.0 0.0 0.0 0.0071141103296881985 0.0 26-27 0.0 0.0 0.0 0.008807946122471103 0.0 28-29 0.0 0.0 0.0 0.013211919183706655 0.0 30-31 0.0 0.0 0.0 0.03692562028266732 0.0 32-33 0.0 0.0 0.0 0.07147987045543856 0.0 34-35 0.0 0.0 0.0 0.10874425789666248 0.0 36-37 0.0 0.0 0.0 0.15515535861891405 0.0 38-39 0.0 0.0 0.0 0.2127457755735328 0.0 40-41 0.0 0.0 0.0 0.2720300283209345 0.0 42-43 0.0 0.0 0.0 0.3377508570809111 0.0 44-45 0.0 0.0 0.0 0.4210875780858301 0.0 46-47 0.0 0.0 0.0 0.5017141618222962 0.0 48-49 0.0 0.0 0.0 0.5918262259983469 0.0 50-51 0.0 0.0 0.0 0.6843096602842934 0.0 52-53 0.0 0.0 0.0 0.7778093960459097 0.0 54-55 0.0 0.0 0.0 0.8970554358578262 0.0 56-57 0.0 0.0 0.0 1.0179953114625255 0.0 58-59 0.0 0.0 0.0 1.179248478935458 0.0 60-61 0.0 0.0 0.0 1.3293223301760233 0.0 62-63 0.0 0.0 0.0 1.4773635784652492 0.0 64-65 0.0 0.0 0.0 1.6491185278534357 0.0 66-67 0.0 0.0 0.0 1.855088960255837 0.0 68-69 0.0 0.0 0.0 2.1057766575877066 0.0 70-71 0.0 0.0 0.0 2.351382847541228 0.0 72-73 0.0 0.0 0.0 2.5814057482011465 0.0 74-75 0.0 0.0 0.0 2.8280282396303376 0.0 76-77 0.0 0.0 0.0 3.104801008171064 0.0 78-79 0.0 0.0 0.0 3.4472946054717672 0.0 80-81 0.0 0.0 0.0 3.81756710977411 0.0 82-83 0.0 0.0 0.0 4.13160426575606 0.0 84-85 0.0 0.0 0.0 4.462918546824397 0.0 86-87 0.0 0.0 0.0 4.842337764407768 0.0 88-89 0.0 0.0 0.0 5.265457945444936 0.0 90-91 0.0 0.0 0.0 5.696708538287464 0.0 92-93 0.0 0.0 0.0 6.1232163909101995 0.0 94-95 0.0 0.0 0.0 6.598167947206527 0.0 96-97 0.0 0.0 0.0 7.101914711980162 0.0 98-99 0.0 0.0 0.0 7.661219290757077 0.0 100-101 0.0 0.0 0.0 8.286244698293968 0.0 102-103 0.0 0.0 0.0 8.943452985893735 0.0 104-105 0.0 0.0 0.0 9.515969483854358 0.0 106-107 0.0 0.0 0.0 10.122701464829195 0.0 108-109 0.0 0.0 0.0 10.761616325866905 0.0 110-111 0.0 0.0 0.0 11.544168462132607 0.0 112-113 0.0 0.0 0.0 12.302668130140791 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGCAG 40 1.8189894E-12 119.076645 5 CAATGCA 20 8.445197E-4 89.30748 4 CCACGCA 40 1.8804712E-8 89.30748 4 TATAACG 20 8.445197E-4 89.30748 2 ACGCAGG 20 8.467988E-4 89.24695 8 GAGTCTT 25 0.0020425392 71.49447 1 GGACATG 25 0.0020425392 71.49447 1 AATGCAG 25 0.0020480459 71.445984 5 TAGGCAT 25 0.0020480459 71.445984 5 GGCATTC 25 0.0020535635 71.39756 7 GGTATCA 2430 0.0 70.12146 1 GACATAT 45 3.940606E-6 66.19858 1 TTAGGCA 30 0.004218474 59.538315 4 GTCAGGC 30 0.004218474 59.538315 2 TGGGACC 80 5.675247E-10 59.497967 6 GCATTCC 30 0.0042298203 59.497963 8 AGGCATT 30 0.0042298203 59.497963 6 GGACCAC 30 0.0042298203 59.497963 8 GGATTTC 30 0.0042298203 59.497963 8 ACATATA 55 1.3001216E-5 54.125744 2 >>END_MODULE