FastQCFastQC Report
Thu 26 May 2016
SRR936261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240012
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17930.14459537488346888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.062.790131
GTACATG116100.058.1100731
TACATGG118450.055.601072
ACATGGG118750.053.7071083
CATGGGA83750.050.862634
GTATCAA24800.047.978752
ATGGGAT32850.047.450035
CATGGGG32450.045.284844
ATGGGAG32050.043.9937445
TCAACGC29100.041.5024455
ATCAACG29450.040.605174
CAACGCA30000.040.4556856
AACGCAG31700.037.7231067
AGTACAT62500.036.6480872
ATGGGGG18350.035.339555
GAGTACA67050.035.225921
GCGTTAC700.002790531133.996371
TATCAAC35450.033.7326473
ATGGGAC17700.031.5954975
ATGGGCG2109.276846E-1131.163345
Produced by FastQC (version 0.11.5)