##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936261_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1240012 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.460779411812144 31.0 30.0 34.0 25.0 34.0 2 29.412808101857078 31.0 30.0 34.0 23.0 34.0 3 29.661898433240967 31.0 30.0 34.0 25.0 34.0 4 33.00673461224569 35.0 35.0 37.0 25.0 37.0 5 33.345540204449634 37.0 35.0 37.0 28.0 37.0 6 33.20254239475102 37.0 35.0 37.0 26.0 37.0 7 33.19877872149625 37.0 35.0 37.0 26.0 37.0 8 33.06967916439518 37.0 35.0 37.0 26.0 37.0 9 34.365823072679945 39.0 35.0 39.0 23.0 39.0 10-11 34.28274242507331 39.0 35.0 39.0 25.0 39.0 12-13 34.2755562042948 39.0 35.0 39.0 24.0 39.0 14-15 35.39955137530927 40.0 36.0 41.0 24.5 41.0 16-17 35.43594215217272 39.5 35.5 41.0 25.0 41.0 18-19 35.47931270019968 39.5 35.0 41.0 25.0 41.0 20-21 35.61170456414938 40.0 35.0 41.0 26.0 41.0 22-23 35.518290145579236 39.0 35.0 41.0 26.0 41.0 24-25 35.5024447344058 39.0 34.5 41.0 26.5 41.0 26-27 35.610053370451254 39.0 35.0 41.0 27.0 41.0 28-29 35.50515599849034 39.0 35.0 41.0 27.0 41.0 30-31 35.43866349680487 39.0 35.0 41.0 27.0 41.0 32-33 35.27718804334152 39.0 34.0 41.0 26.0 41.0 34-35 35.235967474508314 39.0 34.0 41.0 26.0 41.0 36-37 35.125443544094736 39.0 34.0 41.0 25.5 41.0 38-39 34.88657609765067 39.0 34.0 40.5 25.0 41.0 40-41 34.991520646574386 39.0 34.0 40.5 25.5 41.0 42-43 34.90355577204092 39.0 34.0 41.0 25.0 41.0 44-45 34.78068881591469 39.0 34.0 40.5 24.5 41.0 46-47 34.76290713315677 39.0 34.0 40.0 25.0 41.0 48-49 34.67883093066841 39.0 33.0 40.0 24.0 41.0 50-51 34.55721275277981 38.5 33.0 40.0 24.0 41.0 52-53 34.42035722234946 38.0 33.0 40.0 24.0 41.0 54-55 34.26073457353638 38.0 33.0 40.0 24.0 41.0 56-57 34.080785911749246 38.0 33.0 40.0 23.0 41.0 58-59 33.851176843449906 38.0 33.0 40.0 23.0 41.0 60-61 33.61699685164337 37.5 32.0 40.0 22.0 41.0 62-63 33.38514707922182 37.0 32.0 40.0 22.0 41.0 64-65 33.10289900420318 37.0 32.0 40.0 20.5 41.0 66-67 32.81591871691565 36.0 31.0 39.5 20.0 41.0 68-69 32.535256513646644 36.0 31.0 39.0 19.0 41.0 70-71 32.20520809476037 35.0 31.0 39.0 18.0 40.0 72-73 31.871419792711684 35.0 31.0 38.5 17.0 40.0 74-75 31.52940495737138 35.0 31.0 37.5 14.0 39.5 76-77 31.194222313977605 35.0 30.5 37.0 12.5 39.0 78-79 30.856809450231125 35.0 30.0 36.5 9.0 39.0 80-81 30.521890917184674 34.5 30.0 36.0 7.0 38.5 82-83 30.21627371348019 34.0 30.0 36.0 7.0 37.0 84-85 30.222714780179547 34.0 30.0 35.0 4.5 37.0 86-87 30.078684319184006 34.0 30.0 35.0 2.0 36.5 88-89 29.890467995471013 34.0 30.0 35.0 2.0 36.0 90-91 29.71632048722109 34.0 30.0 35.0 2.0 36.0 92-93 29.571669467714827 34.0 30.0 35.0 2.0 36.0 94-95 29.43601634500311 34.0 30.0 35.0 2.0 35.0 96-97 29.323914607277995 34.0 30.0 35.0 2.0 35.0 98-99 29.211051183375645 34.0 30.0 35.0 2.0 35.0 100-101 29.098196227133286 34.0 29.5 35.0 2.0 35.0 102-103 28.11361381986626 33.0 28.0 34.5 2.0 35.0 104-105 28.775850959506844 34.0 29.0 35.0 2.0 35.0 106-107 28.88077373444773 34.0 29.0 35.0 2.0 35.0 108-109 28.82781134376119 34.0 29.0 35.0 2.0 35.0 110-111 28.708737899310652 34.0 29.0 35.0 2.0 35.0 112-113 28.65333319354974 34.0 29.0 35.0 2.0 35.0 114-115 28.53261903917059 34.0 29.0 35.0 2.0 35.0 116-117 28.399022751392728 34.0 29.0 35.0 2.0 35.0 118-119 28.19069452553685 34.0 29.0 35.0 2.0 35.0 120-121 27.991512178914398 34.0 29.0 35.0 2.0 35.0 122-123 27.688584868533532 34.0 28.5 35.0 2.0 35.0 124-125 25.695331980658253 31.5 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48480842249523803 1101 2 0.4993469878396901 1101 3 0.4985397891786043 1101 4 0.5321714960760886 1101 5 0.2407971706208727 1101 6 0.33574829438880727 1101 7 0.3151750915993361 1101 8 0.28746232775746705 1101 9 0.5145947763136576 1101 10-11 0.5420682156080971 1101 12-13 0.4127553056323521 1101 14-15 0.5467277722556716 1101 16-17 0.5748283589144378 1101 18-19 0.49664714098820895 1101 20-21 0.3814785878910598 1101 22-23 0.6307247161282703 1101 24-25 0.7776154746797985 1101 26-27 0.5108736341064244 1101 28-29 0.7763030079944286 1101 30-31 0.7300392071776685 1101 32-33 0.637479440859785 1101 34-35 0.5051940342793486 1101 36-37 0.5378214854028087 1101 38-39 0.7253968202155221 1101 40-41 0.6005811683364257 1101 42-43 0.7563731818171817 1101 44-45 0.6603265650174421 1101 46-47 0.436230762593361 1101 48-49 0.5622812934268566 1101 50-51 0.49587807628039826 1101 52-53 0.4149142725079997 1101 54-55 0.6338805149697393 1101 56-57 0.5838666167321023 1101 58-59 0.5691951916932254 1101 60-61 0.672963617969252 1101 62-63 0.6311976390344043 1101 64-65 0.6569247942758238 1101 66-67 0.6287552546825381 1101 68-69 0.5366806442037557 1101 70-71 0.4498700278546224 1101 72-73 0.5451031689840207 1101 74-75 0.4286282886698487 1101 76-77 0.4307135999874383 1101 78-79 0.4655574770982298 1101 80-81 0.3296524957935496 1101 82-83 0.3217741135578791 1101 84-85 0.16490685351838863 1101 86-87 0.2968356272927366 1101 88-89 0.3958970729769504 1101 90-91 0.28471868201714656 1101 92-93 0.2946140589864399 1101 94-95 0.3743934786587779 1101 96-97 0.33184656235107113 1101 98-99 0.36924479723524684 1101 100-101 0.36607057884403105 1101 102-103 0.46922750168574723 1101 104-105 0.45054837006200543 1101 106-107 0.4764752709423803 1101 108-109 0.4149123283470679 1101 110-111 0.4123215182730817 1101 112-113 0.43812415836054086 1101 114-115 0.48719780643234145 1101 116-117 0.5083063538967814 1101 118-119 0.4202008980673142 1101 120-121 0.4076526265100462 1101 122-123 0.37463983188661487 1101 124-125 0.3037636619655082 1102 1 0.48216871543385054 1102 2 0.5252858818544794 1102 3 0.4693894531035703 1102 4 0.5111679237180979 1102 5 0.4405695280879982 1102 6 0.3956684880689707 1102 7 0.24620860447060267 1102 8 0.31768121683035844 1102 9 0.4761276569905917 1102 10-11 0.4618648356685071 1102 12-13 0.4134032719643912 1102 14-15 0.44140077926632415 1102 16-17 0.4119210798735864 1102 18-19 0.3723936166033823 1102 20-21 0.47367644237332485 1102 22-23 0.5591186533714847 1102 24-25 0.6532436602952032 1102 26-27 0.4392862348829354 1102 28-29 0.4636992085196283 1102 30-31 0.37038582316364455 1102 32-33 0.4068303704877465 1102 34-35 0.3225077472447211 1102 36-37 0.36524018731464025 1102 38-39 0.4607680273781156 1102 40-41 0.47259980032571747 1102 42-43 0.5673324874285939 1102 44-45 0.44549786208042974 1102 46-47 0.39308961107964535 1102 48-49 0.4802337723909531 1102 50-51 0.41511833904191775 1102 52-53 0.5439695822832249 1102 54-55 0.4795121967118092 1102 56-57 0.43980214605355883 1102 58-59 0.5254878253806439 1102 60-61 0.6677513662796741 1102 62-63 0.5366911580567475 1102 64-65 0.5713306761803452 1102 66-67 0.5343944018379219 1102 68-69 0.5030539516710064 1102 70-71 0.4276149472991335 1102 72-73 0.374952861824319 1102 74-75 0.42762453230519526 1102 76-77 0.4432770494289784 1102 78-79 0.4494802889303813 1102 80-81 0.4610398271974425 1102 82-83 0.3784736648444813 1102 84-85 0.4140307189201451 1102 86-87 0.4754473495688458 1102 88-89 0.5197212046626376 1102 90-91 0.43319536968686023 1102 92-93 0.554723385232883 1102 94-95 0.5557388828294201 1102 96-97 0.485585709243594 1102 98-99 0.5304991706794091 1102 100-101 0.6118339567858371 1102 102-103 0.6753283741402107 1102 104-105 0.5684265891929172 1102 106-107 0.6048962834522058 1102 108-109 0.5858504469051908 1102 110-111 0.48283300423205944 1102 112-113 0.42028182052956 1102 114-115 0.5014871069704299 1102 116-117 0.45543414126919046 1102 118-119 0.6052190752972777 1102 120-121 0.5689214576208421 1102 122-123 0.5647800975396677 1102 124-125 0.49816525914337006 1103 1 0.4112084097545079 1103 2 0.3746216102960247 1103 3 0.37801168591498424 1103 4 0.369712853680511 1103 5 0.37841218557132805 1103 6 0.1851218087997708 1103 7 0.2581467872710732 1103 8 0.17133535045586967 1103 9 0.22681512724113873 1103 10-11 0.4927341178309632 1103 12-13 0.3576297497184626 1103 14-15 0.29964997815383754 1103 16-17 0.3211492740173796 1103 18-19 0.36026877315536865 1103 20-21 0.39369973511417555 1103 22-23 0.4896585559056206 1103 24-25 0.562260505994125 1103 26-27 0.43796759300960986 1103 28-29 0.5642737680187935 1103 30-31 0.47479221617979306 1103 32-33 0.580303423691646 1103 34-35 0.5006885935894445 1103 36-37 0.44277802756865725 1103 38-39 0.4928534121498487 1103 40-41 0.5700157586224108 1103 42-43 0.5887812196252966 1103 44-45 0.5210512736727679 1103 46-47 0.39552651873577815 1103 48-49 0.3931349621599125 1103 50-51 0.32382208077150665 1103 52-53 0.36279393631198076 1103 54-55 0.47346238013300024 1103 56-57 0.46700744559622365 1103 58-59 0.4312883343933862 1103 60-61 0.35098824739317536 1103 62-63 0.40422153188486476 1103 64-65 0.31357047855257036 1103 66-67 0.2947896626168287 1103 68-69 0.22485565405791874 1103 70-71 0.233051069648198 1103 72-73 0.36423456970785395 1103 74-75 0.4308288516508654 1103 76-77 0.4298006470445266 1103 78-79 0.44731115897015883 1103 80-81 0.4304475969828161 1103 82-83 0.3933176928271216 1103 84-85 0.41460167321715247 1103 86-87 0.36231780420573756 1103 88-89 0.31529762162464436 1103 90-91 0.38813439213368284 1103 92-93 0.35500472162384966 1103 94-95 0.4903253635303564 1103 96-97 0.4212399045232864 1103 98-99 0.372290229882573 1103 100-101 0.5796506045643781 1103 102-103 0.6649103849129716 1103 104-105 0.5225944118754917 1103 106-107 0.5952801904567977 1103 108-109 0.5328898966990927 1103 110-111 0.4376293860720821 1103 112-113 0.618858183674778 1103 114-115 0.5764271117042199 1103 116-117 0.5494306437006138 1103 118-119 0.555702146260721 1103 120-121 0.5552934016242155 1103 122-123 0.5256047431195583 1103 124-125 0.465274712558152 1104 1 0.12946589729650881 1104 2 0.31100053735938715 1104 3 0.2953995639187248 1104 4 0.19183183100714984 1104 5 0.2816832191050054 1104 6 0.08400558771977273 1104 7 -8.988378244936257E-4 1104 8 -0.029203352657553694 1104 9 0.0857054929241059 1104 10-11 0.2879289174247788 1104 12-13 0.21628016903649439 1104 14-15 0.27031496676421796 1104 16-17 0.33880650357782116 1104 18-19 0.16773024255240898 1104 20-21 0.13528847802262334 1104 22-23 0.24515752280321834 1104 24-25 0.35790030415688534 1104 26-27 0.15076159591876603 1104 28-29 0.2300931730929534 1104 30-31 0.12066784398064101 1104 32-33 0.3148055153809608 1104 34-35 0.16171808895715145 1104 36-37 0.12707134239632722 1104 38-39 0.2080151211011767 1104 40-41 0.1771576916020976 1104 42-43 0.27621853755815096 1104 44-45 0.26036601508351964 1104 46-47 0.17586032647287908 1104 48-49 0.07089718785847765 1104 50-51 0.2603907693391605 1104 52-53 0.3032370529486528 1104 54-55 0.23434602574946695 1104 56-57 0.11894956049602712 1104 58-59 0.20659385433599198 1104 60-61 0.23786686668218948 1104 62-63 0.15877718023013898 1104 64-65 0.1106005541827173 1104 66-67 0.27324506670297666 1104 68-69 0.13919558591628345 1104 70-71 0.18314418608883898 1104 72-73 0.25699736390679817 1104 74-75 0.22733629900577057 1104 76-77 0.25249792647618463 1104 78-79 0.1633620809209404 1104 80-81 0.17933196400666063 1104 82-83 0.22812951920318625 1104 84-85 0.3324078890826243 1104 86-87 0.3853773556001343 1104 88-89 0.4211806203268935 1104 90-91 0.413154289646787 1104 92-93 0.4428082322116751 1104 94-95 0.343756557341937 1104 96-97 0.3465397394621448 1104 98-99 0.34205581169369736 1104 100-101 0.3432211005711565 1104 102-103 0.47240125950179035 1104 104-105 0.30649448127174495 1104 106-107 0.3102070499431093 1104 108-109 0.34379569422670286 1104 110-111 0.5012202696784911 1104 112-113 0.4007057706847412 1104 114-115 0.523876505369163 1104 116-117 0.5996155043396065 1104 118-119 0.6249349059967777 1104 120-121 0.6442386177915829 1104 122-123 0.6096636005514284 1104 124-125 0.6827229692223469 1105 1 0.169579981685402 1105 2 0.3062775250027876 1105 3 0.2231580649596694 1105 4 0.1579387520758999 1105 5 0.14617267367079734 1105 6 0.207873068839163 1105 7 0.21842662043226113 1105 8 0.16763848235222412 1105 9 0.35997575192718756 1105 10-11 0.22796216661832602 1105 12-13 0.2275832425533082 1105 14-15 0.14849886937560086 1105 16-17 0.18770785667765466 1105 18-19 0.17903051988321295 1105 20-21 0.02265975814199095 1105 22-23 -0.07843860117458235 1105 24-25 0.14329811245546153 1105 26-27 0.09200000127930252 1105 28-29 0.1851232575123518 1105 30-31 0.1940870456441175 1105 32-33 0.34006625972567406 1105 34-35 0.32338708154216533 1105 36-37 0.2496378344284622 1105 38-39 0.32177731310763846 1105 40-41 0.039320465215254785 1105 42-43 0.4259243025310866 1105 44-45 0.4568499382849964 1105 46-47 0.2763561410908295 1105 48-49 0.38756143963723844 1105 50-51 0.30444315461291893 1105 52-53 0.28294017425482565 1105 54-55 0.21680237347299425 1105 56-57 0.35044745191758864 1105 58-59 0.388904164890576 1105 60-61 0.3743095588500722 1105 62-63 0.3474477971450298 1105 64-65 0.35856785405841407 1105 66-67 0.2507799999630933 1105 68-69 0.23349390845888252 1105 70-71 0.2864369493052692 1105 72-73 0.32234143459438513 1105 74-75 0.279717251386721 1105 76-77 0.3202576476710206 1105 78-79 0.3785522342790344 1105 80-81 0.3386693187638592 1105 82-83 0.3948796901518321 1105 84-85 0.44574849249956827 1105 86-87 0.46023824534652036 1105 88-89 0.44717421960293535 1105 90-91 0.5371943717021388 1105 92-93 0.5132038671111303 1105 94-95 0.4638955786259942 1105 96-97 0.45966757854418105 1105 98-99 0.351669607567036 1105 100-101 0.37241010495100113 1105 102-103 0.5160197275477394 1105 104-105 0.46506860434968544 1105 106-107 0.40725211683550455 1105 108-109 0.3802826169731688 1105 110-111 0.5032050533426613 1105 112-113 0.4931138714897898 1105 114-115 0.4398856870271999 1105 116-117 0.6045901734364136 1105 118-119 0.5469284340971754 1105 120-121 0.5766957281112859 1105 122-123 0.7553100660452223 1105 124-125 0.7765806382708575 1106 1 -0.4228352057627305 1106 2 -0.39387533784647744 1106 3 -0.2486891451034552 1106 4 -0.027875180570262614 1106 5 -0.09441213432404538 1106 6 -0.2277865579000391 1106 7 -0.20135202970624988 1106 8 -0.28475301869614356 1106 9 -0.2475130593122543 1106 10-11 -0.3470613906657434 1106 12-13 -0.31319161867264 1106 14-15 -0.5153729911510965 1106 16-17 -0.3561017295799829 1106 18-19 -0.48916507063877646 1106 20-21 -0.45864669516141987 1106 22-23 -0.4436510623069694 1106 24-25 -4.154689505603116 1106 26-27 -1.1977353520647682 1106 28-29 -0.8357174071448412 1106 30-31 -0.6752954724966429 1106 32-33 -5.635084539804247 1106 34-35 -1.2248000329145867 1106 36-37 -0.5452958178290643 1106 38-39 -0.21479394634754811 1106 40-41 -0.8465556961297338 1106 42-43 -0.5202131889687962 1106 44-45 -0.46631736577435134 1106 46-47 -0.5787863089578593 1106 48-49 -0.4366822335674101 1106 50-51 -0.43142108251841904 1106 52-53 -0.2080446392084454 1106 54-55 -0.3662755977195289 1106 56-57 -0.30458340166876496 1106 58-59 -0.22469637547123966 1106 60-61 -0.166226691518915 1106 62-63 -0.27769973651745516 1106 64-65 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-0.7940623115214187 2216 102-103 -0.8234204618924501 2216 104-105 -0.8040289647478858 2216 106-107 -0.9257334161500275 2216 108-109 -0.8604229737324225 2216 110-111 -0.8362388361012236 2216 112-113 -0.7426744142984951 2216 114-115 -0.761934714793199 2216 116-117 -0.6852829164366732 2216 118-119 -0.7001448629761242 2216 120-121 -0.6425257911102342 2216 122-123 -0.5897689290337738 2216 124-125 -0.8233435116532917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37115.0 3 6749.0 4 5058.0 5 4000.0 6 4274.0 7 3911.0 8 3874.0 9 4406.0 10 5138.0 11 5995.0 12 6384.0 13 6759.0 14 6505.0 15 6508.0 16 6134.0 17 5701.0 18 5073.0 19 4625.0 20 4549.0 21 4611.0 22 5496.0 23 7717.0 24 11673.0 25 17249.0 26 20656.0 27 18645.0 28 20550.0 29 25617.0 30 29932.0 31 36761.0 32 47048.0 33 62032.0 34 84849.0 35 122421.0 36 201065.0 37 289863.0 38 100770.0 39 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.72289856194476 18.33141399087748 8.95679705292224 21.98889039425552 2 18.746749010287843 21.966085675322798 36.88229179351407 22.404873520875284 3 17.099956532573554 30.074781715294 29.891799172425358 22.933462579707083 4 10.598488556919436 16.680873575713687 36.7314861932231 35.98915167414378 5 10.308748755045343 44.693287527066424 34.00768545288123 10.990278265006996 6 30.071975516227756 37.9567824323289 19.007020132983335 12.964221918460005 7 27.929851782616655 36.0100741691754 20.931884842214092 15.128189205993856 8 26.980629791846273 35.50266653870936 19.46545031963787 18.051253349806494 9 27.816478869150345 14.692642186859873 18.173321032139018 39.317557911850756 10-11 23.476451249867438 28.75382613509851 28.869996286264865 18.89972632876919 12-13 24.014495319102263 27.788608988888186 28.169016356226457 20.02787933578309 14-15 22.116427590422756 28.810643066305047 25.20457974759291 23.868349595679287 16-17 21.886854991677314 31.09689318571853 25.664847288352107 21.351404534252055 18-19 21.15861125683426 30.110457025874116 27.38469714742402 21.346234569867605 20-21 22.748708147090785 29.138193482569307 26.916145993995883 21.196952376344022 22-23 22.53145881238191 29.476449171550307 26.753287527612972 21.238804488454814 24-25 21.980938525211535 29.40822814414333 27.148448210789137 21.462385119856 26-27 21.850679055978922 29.21878219522925 27.152561686815297 21.777977061976532 28-29 21.767063576869262 29.161165972443438 27.26079540643309 21.81097504425421 30-31 21.93709183401909 28.953474118506644 27.476052380729648 21.633381666744626 32-33 21.647688713995215 29.154554688479717 27.27369502129652 21.924061576228546 34-35 21.97331531182069 29.108479479225597 27.289890036245986 21.628315172707733 36-37 22.151335239781872 28.76696973069398 27.033464462154093 22.04823056737005 38-39 21.827768340042347 28.8976962472132 27.47527517170384 21.799260241040614 40-41 21.839030867450727 28.98171807881561 27.544454798424674 21.63479625530899 42-43 21.695943006198817 28.77867603445015 27.548227028426336 21.977153930924693 44-45 21.699231973019256 28.835144966251892 27.508809507776437 21.956813552952408 46-47 21.910697040506292 28.95529610058125 27.134881864204825 21.999124994707632 48-49 21.814370253047162 28.905424651171042 27.259908419797966 22.020296675983825 50-51 21.738998947576402 28.755720789196733 27.4095460062339 22.095734256992973 52-53 21.88686746822775 28.89111828496359 27.392445304303152 21.829568942505507 54-55 21.593255139532708 28.88299639676257 27.472548298542897 22.051200165161823 56-57 21.86235634060995 29.137805756294966 27.226513892349914 21.77332401074517 58-59 22.074986915354238 28.674083853022935 27.128885978501664 22.122043253121163 60-61 21.77966504919746 28.776825539938212 27.615900929271948 21.827608481592385 62-63 21.60915647007353 28.82183230208125 27.545499083667508 22.023512144177708 64-65 21.642154550124637 28.99038628256958 27.35744141729147 22.010017750014317 66-67 21.616951853458772 29.25264071302881 27.159828258895523 21.970579174616898 68-69 21.688082479946743 28.93225500913097 27.291905995957293 22.087756514964994 70-71 21.49405487706531 28.840217159889292 27.521757924142427 22.143970038902975 72-73 21.718897428246546 28.90254917299054 27.248167353489084 22.130386045273827 74-75 21.380328691162724 29.138094965439766 27.41387360898187 22.067702734415636 76-77 21.295974464701466 29.07309543721054 27.470002233854757 22.160927864233244 78-79 21.58840910703802 29.009611228314657 27.359742840368774 22.042236824278554 80-81 21.368387436522536 29.025602274825868 27.229644671360724 22.37636561729087 82-83 21.609359286908404 29.128740194627618 27.245689345803136 22.016211172660842 84-85 21.37501476804393 28.933551665170036 27.47896486193441 22.21246870485162 86-87 21.263750930438295 28.868143486286773 27.560570222587454 22.30753536068748 88-89 21.512920098773716 28.619700227256967 27.721680378965907 22.145699295003414 90-91 21.400556045023922 28.998735894742573 27.51910774733217 22.081600312901333 92-93 21.46535974119553 29.00417295190077 27.374548340153847 22.15591896674985 94-95 21.18092881733732 28.789232641428637 27.48125615419913 22.548582387034912 96-97 21.303727562608568 29.01300477578334 27.46176228179541 22.22150537981268 98-99 21.20009290292612 28.945753400795482 27.44075825561853 22.41339544065987 100-101 21.072584817232517 28.91949182442483 27.597722427620226 22.41020093072243 102-103 20.97633064516129 28.980040322580646 27.390120967741936 22.653508064516128 104-105 21.015641065963443 28.83210148346176 27.7384768609804 22.4137805895944 106-107 20.96786745531972 28.885338722561087 27.678730198156536 22.468063623962657 108-109 21.068092036688547 29.186402802245638 27.149516675530876 22.595988485534942 110-111 20.80957836006108 29.27161092690464 27.334237363039698 22.584573349994578 112-113 20.97146460817868 29.076438463101734 27.494669372034288 22.4574275566853 114-115 20.722939175771117 29.329298411501544 27.213424112354158 22.73433830037318 116-117 20.63168522915918 29.234665631183216 27.4079728193658 22.725676320291807 118-119 20.559881289843908 29.21479522094847 27.360755486917288 22.86456800229033 120-121 20.479911096810042 29.105028586682018 27.346561553805827 23.06849876270211 122-123 20.674289506907854 29.112626073436203 27.209926762185365 23.003157657470574 124-125 20.699674598086297 29.067705372155757 27.024003935468 23.20861609428994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1428.0 1 1696.0 2 1888.5 3 1559.0 4 1125.0 5 866.5 6 708.5 7 637.0 8 606.5 9 581.0 10 573.0 11 555.0 12 559.0 13 593.5 14 684.5 15 728.5 16 771.0 17 924.5 18 1079.0 19 1276.0 20 1646.5 21 2178.0 22 3009.5 23 4180.5 24 5457.5 25 7010.5 26 9201.0 27 11329.5 28 12930.0 29 15008.0 30 18155.5 31 21017.5 32 23492.0 33 26539.5 34 30272.0 35 32684.5 36 34765.0 37 37299.0 38 38183.5 39 38431.5 40 38490.0 41 40225.0 42 41754.5 43 42977.0 44 45803.0 45 48004.5 46 48018.5 47 46970.5 48 46155.5 49 44365.5 50 42272.0 51 39787.0 52 37385.5 53 35681.0 54 33954.0 55 31883.0 56 28883.0 57 26155.5 58 24074.0 59 21494.5 60 18280.0 61 14758.5 62 11959.5 63 9483.5 64 7177.0 65 5603.5 66 4266.0 67 3277.0 68 2529.5 69 1797.0 70 1295.0 71 1004.0 72 843.5 73 717.5 74 529.5 75 366.0 76 286.5 77 195.5 78 115.5 79 83.0 80 44.5 81 30.5 82 26.0 83 22.0 84 18.0 85 12.0 86 11.5 87 6.0 88 5.0 89 5.5 90 4.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.225775234433215E-4 2 4.032219043041519E-4 3 2.4193314258249112E-4 4 4.032219043041519E-4 5 5.645106660258127E-4 6 5.645106660258127E-4 7 7.257994277474735E-4 8 0.0012096657129124556 9 0.001935465140659929 10-11 0.0016532098076470227 12-13 0.0020564317119511747 14-15 0.002499975806685742 16-17 0.0022580426641032507 18-19 0.0022177204736728356 20-21 0.002379009235394496 22-23 0.08153146905029951 24-25 0.001935465140659929 26-27 0.001491921045925362 28-29 0.0013709544746341165 30-31 0.0011290213320516254 32-33 0.0016532098076470227 34-35 0.0010080547607603798 36-37 8.064438086083038E-4 38-39 0.0010886991416212102 40-41 0.0013306322842037014 42-43 0.0014112766650645316 44-45 0.0013306322842037014 46-47 0.0015725654267861923 48-49 4.8386628516498224E-4 50-51 0.0013709544746341165 52-53 0.0012096657129124556 54-55 0.001290310093773286 56-57 0.001048376951190795 58-59 7.257994277474735E-4 60-61 7.257994277474735E-4 62-63 0.0011693435224820405 64-65 0.001048376951190795 66-67 4.8386628516498224E-4 68-69 1.2096657129124556E-4 70-71 3.225775234433215E-4 72-73 1.6128876172166075E-4 74-75 2.4193314258249112E-4 76-77 2.4193314258249112E-4 78-79 3.225775234433215E-4 80-81 4.032219043041519E-4 82-83 2.4193314258249112E-4 84-85 2.8225533301290634E-4 86-87 4.032219043041519E-4 88-89 4.8386628516498224E-4 90-91 3.6289971387373673E-4 92-93 2.0161095215207595E-4 94-95 4.032219043041519E-4 96-97 4.8386628516498224E-4 98-99 6.45155046886643E-4 100-101 6.048328564562278E-4 102-103 9.677325703299645E-4 104-105 5.645106660258127E-4 106-107 6.048328564562278E-4 108-109 5.241884755953975E-4 110-111 6.854772373170582E-4 112-113 6.45155046886643E-4 114-115 3.6289971387373673E-4 116-117 9.274103798995495E-4 118-119 0.0013709544746341165 120-121 9.274103798995495E-4 122-123 8.46765999038719E-4 124-125 5.645106660258127E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1240012.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.62399663028619 #Duplication Level Percentage of deduplicated Percentage of total 1 55.07568981792058 18.518648088484493 2 13.364809623459392 8.987566274872304 3 9.165607592327971 9.24553076396884 4 6.729189433057092 9.050489712866765 5 4.525627374097298 7.608483978828925 6 3.008632894325662 6.069735738034457 7 2.006462774396298 4.722570829556708 8 1.3257779116086466 3.56623616259496 9 0.913817852758166 2.7653587561652344 >10 3.6115291919223305 20.282346345407376 >50 0.19256772330855282 4.43391944511133 >100 0.07884982107518805 4.344496934870874 >500 0.0011983247856844 0.26012817699650753 >1k 2.3966495713688004E-4 0.14448879224124472 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1793 0.14459537488346888 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.032219043041519E-5 0.0 24-25 0.0 0.0 0.0 2.0161095215207595E-4 0.0 26-27 0.0 0.0 0.0 2.8225533301290634E-4 0.0 28-29 0.0 0.0 0.0 6.45155046886643E-4 0.0 30-31 0.0 0.0 0.0 0.002701586758837818 0.0 32-33 0.0 0.0 0.0 0.0076612161817788856 0.0 34-35 0.0 0.0 0.0 0.01508049922097528 0.0 36-37 0.0 0.0 0.0 0.02850778863430354 0.0 38-39 0.0 0.0 0.0 0.05100757089447522 0.0 40-41 0.0 0.0 0.0 0.07399121943981188 0.0 42-43 0.0 0.0 0.0 0.10338609626358455 0.0 44-45 0.0 0.0 0.0 0.1425792653619481 0.0 46-47 0.0 0.0 0.0 0.18757882988229146 0.0 48-49 0.0 0.0 0.0 0.23745737944471507 0.0 50-51 0.0 0.0 0.0 0.28120695606171553 0.0 52-53 0.0 0.0 0.0 0.3320129160040387 0.0 54-55 0.0 0.0 0.0 0.39862517459508456 0.0 56-57 0.0 0.0 0.0 0.4798340661219408 0.0 58-59 0.0 0.0 0.0 0.5769299006783806 0.0 60-61 0.0 0.0 0.0 0.6757192672328978 0.0 62-63 0.0 0.0 0.0 0.7789843969251911 0.0 64-65 0.0 0.0 0.0 0.8953139163169388 0.0 66-67 0.0 0.0 0.0 1.0380141482501783 0.0 68-69 0.0 0.0 0.0 1.2143027648119533 0.0 70-71 0.0 0.0 0.0 1.3985348528885204 0.0 72-73 0.0 0.0 0.0 1.5783718222081722 0.0 74-75 0.0 0.0 0.0 1.7776037651248537 0.0 76-77 0.0 0.0 0.0 2.010504737050932 0.0 78-79 0.0 0.0 0.0 2.283526288455273 0.0 80-81 0.0 0.0 0.0 2.568967074512182 0.0 82-83 0.0 0.0 0.0 2.8617464992274266 0.0 84-85 0.0 0.0 0.0 3.168195146498582 0.0 86-87 0.0 0.0 0.0 3.518474014767599 0.0 88-89 0.0 0.0 0.0 3.915325012983745 0.0 90-91 0.0 0.0 0.0 4.333990316222746 0.0 92-93 0.0 0.0 0.0 4.760074902500943 0.0 94-95 0.0 0.0 0.0 5.214102766747418 0.0 96-97 0.0 0.0 0.0 5.686961093924897 0.0 98-99 0.0 0.0 0.0 6.2271978013116005 0.0 100-101 0.0 0.0 0.0 6.816264681309535 0.0 102-103 0.0 0.0 0.0 7.390452673038649 0.0 104-105 0.0 0.0 0.0 7.999196781966626 0.0 106-107 0.0 0.0 0.0 8.64842437008674 0.0 108-109 0.0 0.0 0.0 9.340837024157832 0.0 110-111 0.0 0.0 0.0 10.051193052970454 0.0 112-113 0.0 0.0 0.0 10.768968364822275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1895 0.0 62.79013 1 GTACATG 11610 0.0 58.110073 1 TACATGG 11845 0.0 55.60107 2 ACATGGG 11875 0.0 53.707108 3 CATGGGA 8375 0.0 50.86263 4 GTATCAA 2480 0.0 47.97875 2 ATGGGAT 3285 0.0 47.45003 5 CATGGGG 3245 0.0 45.28484 4 ATGGGAG 3205 0.0 43.993744 5 TCAACGC 2910 0.0 41.502445 5 ATCAACG 2945 0.0 40.60517 4 CAACGCA 3000 0.0 40.455685 6 AACGCAG 3170 0.0 37.723106 7 AGTACAT 6250 0.0 36.648087 2 ATGGGGG 1835 0.0 35.33955 5 GAGTACA 6705 0.0 35.22592 1 GCGTTAC 70 0.0027905311 33.99637 1 TATCAAC 3545 0.0 33.732647 3 ATGGGAC 1770 0.0 31.595497 5 ATGGGCG 210 9.276846E-11 31.16334 5 >>END_MODULE