FastQCFastQC Report
Thu 26 May 2016
SRR936261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240012
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.6457195575526689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62840.5067692893294581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57310.46217294671341885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.13862769069976744No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.13677286993996832No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.12854714309216364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.083.500811
AGCGTAC551.3069566E-554.117523
GTATCAA62250.053.7436942
GTACATG99050.050.466851
TACATGG101300.049.3628732
ACATGGG102500.049.075353
CATGGGA74600.046.6020054
CATGGGG28350.040.106144
ATCAACG87500.039.4594044
ATGGGAT32300.039.2561455
TCAACGC88400.039.125015
ATGGGAG28400.038.7778745
CAACGCA89950.038.6259776
AACGCAG91200.038.16187
TATCAAC91300.037.8822633
CCGAGTA2005.0931703E-1132.74114
ACGCAGA106450.032.6947488
CGCAGAG107450.032.1699
AGTACAT59850.031.8285142
TACCTGG8750.031.295392
Produced by FastQC (version 0.11.5)