##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936261_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1240012 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.570546897933244 31.0 31.0 34.0 30.0 34.0 2 31.31942190882024 31.0 31.0 34.0 28.0 34.0 3 31.681354696567453 31.0 31.0 34.0 30.0 34.0 4 35.532181946626324 37.0 35.0 37.0 33.0 37.0 5 35.19194411021829 37.0 35.0 37.0 33.0 37.0 6 35.41761450695638 37.0 35.0 37.0 33.0 37.0 7 35.23933236129973 37.0 35.0 37.0 32.0 37.0 8 35.33167098382919 37.0 35.0 37.0 32.0 37.0 9 36.96653177549895 39.0 37.0 39.0 33.0 39.0 10-11 36.88312008270888 39.0 37.0 39.0 32.5 39.0 12-13 36.83506208004439 39.0 37.0 39.0 32.0 39.0 14-15 37.96953577868601 40.0 37.0 41.0 33.0 41.0 16-17 37.88788052051109 40.0 37.0 41.0 32.5 41.0 18-19 38.02648925978136 40.0 37.0 41.0 33.0 41.0 20-21 38.09124064928404 40.0 37.0 41.0 33.0 41.0 22-23 37.98900736444486 40.0 37.0 41.0 33.0 41.0 24-25 37.95435971587372 40.0 37.0 41.0 33.0 41.0 26-27 37.775307013157935 40.0 37.0 41.0 32.0 41.0 28-29 37.61403196098102 40.0 37.0 41.0 32.0 41.0 30-31 37.404295684235315 39.5 36.0 41.0 32.0 41.0 32-33 37.15444689244943 39.0 35.5 41.0 31.0 41.0 34-35 36.93548489853324 39.0 35.0 41.0 31.0 41.0 36-37 36.71003022551395 39.0 35.0 41.0 30.0 41.0 38-39 36.67995793589094 39.0 35.0 41.0 30.5 41.0 40-41 36.67747973406709 39.0 35.0 41.0 30.0 41.0 42-43 36.55319585616913 39.0 35.0 41.0 30.0 41.0 44-45 36.34076726676839 39.0 35.0 41.0 30.0 41.0 46-47 36.0035600461931 39.0 35.0 41.0 29.0 41.0 48-49 35.688713496321 39.0 35.0 41.0 27.0 41.0 50-51 35.509777324735566 39.0 35.0 41.0 27.0 41.0 52-53 35.37736288035923 39.0 35.0 41.0 26.5 41.0 54-55 35.21708862494879 39.0 35.0 41.0 26.0 41.0 56-57 35.03290411705693 39.0 34.5 41.0 26.0 41.0 58-59 34.82013883736609 38.0 34.0 40.0 26.0 41.0 60-61 34.60691267503863 38.0 34.0 40.0 25.5 41.0 62-63 34.401458211694724 38.0 34.0 40.0 25.0 41.0 64-65 34.1521586081425 37.0 34.0 40.0 25.0 41.0 66-67 33.87702417395961 37.0 33.5 40.0 24.0 41.0 68-69 33.57250413705674 36.0 33.0 39.0 23.5 41.0 70-71 33.25409189588488 36.0 33.0 39.0 23.0 41.0 72-73 32.972359541681854 35.5 33.0 39.0 23.0 40.0 74-75 32.77377476992159 35.0 33.0 38.0 23.5 40.0 76-77 32.46318503369322 35.0 33.0 37.0 23.0 39.0 78-79 32.12299719680132 35.0 32.5 37.0 22.0 39.0 80-81 31.83591932981294 35.0 32.0 36.0 22.0 39.0 82-83 31.568538046406005 35.0 32.0 36.0 21.0 37.0 84-85 31.308223630093902 35.0 32.0 35.5 20.5 37.0 86-87 31.09787405283175 35.0 32.0 35.0 20.0 37.0 88-89 30.932553475288948 35.0 32.0 35.0 20.0 36.0 90-91 30.786879481811468 35.0 32.0 35.0 19.5 36.0 92-93 30.648313080841156 35.0 32.0 35.0 19.0 36.0 94-95 30.52953035938362 35.0 32.0 35.0 18.0 35.5 96-97 30.45708307661539 35.0 32.0 35.0 18.0 35.0 98-99 30.364710583445966 34.0 32.0 35.0 17.5 35.0 100-101 30.280470672864457 34.0 31.0 35.0 16.5 35.0 102-103 29.27063246162134 33.5 29.5 34.5 10.5 35.0 104-105 30.00978700206127 34.0 31.0 35.0 11.0 35.0 106-107 30.14402400944507 34.0 31.0 35.0 10.5 35.0 108-109 30.107969116427903 34.0 31.0 35.0 8.5 35.0 110-111 30.070674316055005 34.0 31.0 35.0 7.0 35.0 112-113 29.983895317142093 34.0 31.0 35.0 3.5 35.0 114-115 29.96515517591765 34.0 31.5 35.0 2.0 35.0 116-117 29.863646480840508 34.0 31.0 35.0 2.0 35.0 118-119 29.731786869804488 34.0 31.0 35.0 2.0 35.0 120-121 29.54347417605636 34.0 31.0 35.0 2.0 35.0 122-123 29.318586836256422 34.0 31.0 35.0 2.0 35.0 124-125 27.276584420150773 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21323741501764104 1101 2 0.5864822825324154 1101 3 0.3945183744331331 1101 4 0.2557476306978117 1101 5 0.4843218319677476 1101 6 0.3038735758018234 1101 7 0.4947814439292486 1101 8 0.46380368167078245 1101 9 0.34168681447410165 1101 10-11 0.40508068294127497 1101 12-13 0.40095620535619503 1101 14-15 0.39664875518325005 1101 16-17 0.5878798307865623 1101 18-19 0.477445532226632 1101 20-21 0.3659584615960725 1101 22-23 0.34184638851256466 1101 24-25 0.395415507939866 1101 26-27 0.37824568510897194 1101 28-29 0.4165083318547289 1101 30-31 0.3630518268171201 1101 32-33 0.3541100110102917 1101 34-35 0.3017917446664171 1101 36-37 0.3952015569924825 1101 38-39 0.47949501647397597 1101 40-41 0.426027343858955 1101 42-43 0.41041782978680175 1101 44-45 0.272264529030501 1101 46-47 0.18127138576581814 1101 48-49 0.04877370278818205 1101 50-51 -0.11666551271724757 1101 52-53 0.009118925006347922 1101 54-55 -0.022376956679259763 1101 56-57 -0.23511926985571563 1101 58-59 -0.10990997014470594 1101 60-61 0.1683857108193223 1101 62-63 0.06968905566986194 1101 64-65 0.025808234697997534 1101 66-67 0.11613001026690739 1101 68-69 0.0020251294091622185 1101 70-71 -0.12042276909009075 1101 72-73 -0.038558954164123804 1101 74-75 -0.08033770290701625 1101 76-77 0.028101891838566928 1101 78-79 0.019806129905859393 1101 80-81 -0.09528803686218268 1101 82-83 -0.08674943935236712 1101 84-85 -0.09792612609121676 1101 86-87 -0.08748294605673479 1101 88-89 -0.15062659617863972 1101 90-91 -0.031042758374724144 1101 92-93 -0.0797116118261485 1101 94-95 0.009666536934197723 1101 96-97 -0.04607078564253442 1101 98-99 -0.014594143047268915 1101 100-101 -0.05443977925816057 1101 102-103 0.04764352671302774 1101 104-105 -0.06210803358247219 1101 106-107 -0.03992501656973246 1101 108-109 -0.10588167543735238 1101 110-111 -0.09286653670845624 1101 112-113 -0.035316498548290554 1101 114-115 -0.06507513235402484 1101 116-117 0.0023434463270142203 1101 118-119 -0.035560075081047415 1101 120-121 -0.06472677766055668 1101 122-123 0.0763418081410947 1101 124-125 -0.04120462018330073 1102 1 0.15681071933049395 1102 2 0.5915666495889162 1102 3 0.28218756199112605 1102 4 0.22403276742790723 1102 5 0.3276814256810283 1102 6 0.23496360500630686 1102 7 0.346860062091757 1102 8 0.2697000438589896 1102 9 0.2899775239775195 1102 10-11 0.32627930236554903 1102 12-13 0.29027369834422956 1102 14-15 0.30605754176443156 1102 16-17 0.32496295711239753 1102 18-19 0.32026138624100753 1102 20-21 0.29109666374579746 1102 22-23 0.26374213033544436 1102 24-25 0.33529895286010003 1102 26-27 0.3307066428373844 1102 28-29 0.2990346645230062 1102 30-31 0.24919113037817198 1102 32-33 0.23247647868688404 1102 34-35 0.37117908132764654 1102 36-37 0.3416962299994921 1102 38-39 0.3509123409644701 1102 40-41 0.29055887602988406 1102 42-43 0.18198367394366954 1102 44-45 0.3221978923306281 1102 46-47 0.4324728178634203 1102 48-49 0.4915795678692376 1102 50-51 0.43678816097584416 1102 52-53 0.5045566111488071 1102 54-55 0.3157419957445029 1102 56-57 0.337870391598301 1102 58-59 0.4688251680311524 1102 60-61 0.4538502356483889 1102 62-63 0.4200527317024765 1102 64-65 0.3722651863384172 1102 66-67 0.2944586251435837 1102 68-69 0.31267740675452416 1102 70-71 0.20473107362626308 1102 72-73 0.32194164911488343 1102 74-75 0.30500800618895596 1102 76-77 0.31309154803525274 1102 78-79 0.28385070157776937 1102 80-81 0.3333100163472906 1102 82-83 0.2820005770768006 1102 84-85 0.35692494679908293 1102 86-87 0.23454422909921036 1102 88-89 0.30303881661403054 1102 90-91 0.2829263705640024 1102 92-93 0.3971483669867979 1102 94-95 0.21195728849628281 1102 96-97 0.20637729161924057 1102 98-99 0.27962778476428696 1102 100-101 0.18618534396058806 1102 102-103 0.19632787904899018 1102 104-105 0.22453925981089995 1102 106-107 0.1844301057815585 1102 108-109 0.16160684386100144 1102 110-111 0.15823185165601572 1102 112-113 0.1526573514757601 1102 114-115 0.21775218264698637 1102 116-117 0.24580424370676113 1102 118-119 0.15356026790737687 1102 120-121 0.09082102332834907 1102 122-123 -0.0931508066199207 1102 124-125 -0.0240367982176366 1103 1 0.10575661569608741 1103 2 0.5896459650811998 1103 3 0.23441915599729768 1103 4 0.18875926059553194 1103 5 0.3029599275471142 1103 6 0.23006732249221784 1103 7 0.30072340194058 1103 8 0.29198776929515446 1103 9 0.3058678443232452 1103 10-11 0.4120379178843194 1103 12-13 0.3876317905339519 1103 14-15 0.46634691932366934 1103 16-17 0.5364817347294206 1103 18-19 0.49413310165366653 1103 20-21 0.3513265293449095 1103 22-23 0.3056925760481519 1103 24-25 0.32251145758213084 1103 26-27 0.3771018064325702 1103 28-29 0.22885091416183911 1103 30-31 0.09723839092091424 1103 32-33 0.2851026333312561 1103 34-35 0.2809654286883472 1103 36-37 0.17458426861799836 1103 38-39 0.23544672910679765 1103 40-41 0.1533502377565341 1103 42-43 0.09774956256591594 1103 44-45 0.21376307778169945 1103 46-47 0.1985824546732644 1103 48-49 0.19962076226748593 1103 50-51 0.14516126909376936 1103 52-53 0.21659056692813294 1103 54-55 0.15695439387794607 1103 56-57 0.17905757944134848 1103 58-59 0.1949100073201251 1103 60-61 -0.0029667574980649647 1103 62-63 -0.13657898540306235 1103 64-65 0.08297827964889137 1103 66-67 0.11028360773150325 1103 68-69 2.3426053117958645E-4 1103 70-71 -0.03160076903435538 1103 72-73 0.030139460809422758 1103 74-75 0.035275663711473726 1103 76-77 0.07458326051149555 1103 78-79 0.08898351670248417 1103 80-81 0.1232696328582783 1103 82-83 -0.023185140470765475 1103 84-85 -0.018849191988664415 1103 86-87 0.11458582999706124 1103 88-89 0.1300926976663952 1103 90-91 -0.029300463530340437 1103 92-93 -0.02777760880818647 1103 94-95 -0.04457831600990758 1103 96-97 -0.021429815435933364 1103 98-99 0.11585938713034594 1103 100-101 0.0651789543928949 1103 102-103 0.14283151220451984 1103 104-105 -0.0018236764120267424 1103 106-107 0.048904548294522954 1103 108-109 0.030856219873662383 1103 110-111 -0.05792588064048232 1103 112-113 -0.05284256727656356 1103 114-115 0.07460149198952948 1103 116-117 0.1733132945928837 1103 118-119 0.10870201766350007 1103 120-121 0.0353723611457184 1103 122-123 0.003745924193221839 1103 124-125 0.3037267590405506 1104 1 0.16684413730890313 1104 2 0.41950987785363836 1104 3 0.23941254963087744 1104 4 0.11907287676759637 1104 5 0.37781848857464695 1104 6 0.2190125386595838 1104 7 0.3985587877563006 1104 8 0.37280421424667765 1104 9 0.29672557416402157 1104 10-11 0.21951546211953143 1104 12-13 0.268459213423732 1104 14-15 0.15530311342929792 1104 16-17 0.3487295852099024 1104 18-19 0.334176699751346 1104 20-21 0.2808587515540637 1104 22-23 0.31679362404918265 1104 24-25 0.21864173434379808 1104 26-27 0.27630276935459364 1104 28-29 0.31960121703531286 1104 30-31 -0.04208654428965275 1104 32-33 0.041059929151401775 1104 34-35 0.013052766028629037 1104 36-37 0.09133150551014069 1104 38-39 0.07152807562934527 1104 40-41 0.1798411789971226 1104 42-43 0.23069121874473097 1104 44-45 0.1787501198938628 1104 46-47 0.1196996951332352 1104 48-49 0.17688976488258845 1104 50-51 -0.10728034199708958 1104 52-53 0.021265600352030845 1104 54-55 0.08214993840329043 1104 56-57 -0.05585422458676703 1104 58-59 0.04604994858815559 1104 60-61 -0.020447947977061176 1104 62-63 -0.06250417075379033 1104 64-65 0.005592048335891775 1104 66-67 -0.018708343087332935 1104 68-69 -0.11131688293870923 1104 70-71 -0.09658506666194455 1104 72-73 -0.04255919086451598 1104 74-75 0.08238395715176239 1104 76-77 -0.014265506626820468 1104 78-79 0.04307377887917596 1104 80-81 -0.04143080665132004 1104 82-83 -0.09774220026524105 1104 84-85 -0.005607053956772745 1104 86-87 -0.0495339549921745 1104 88-89 0.04359791414741565 1104 90-91 -0.0536989313238152 1104 92-93 -0.19572242114425364 1104 94-95 -0.03551759624922468 1104 96-97 0.018148004423942155 1104 98-99 -0.11803813381595063 1104 100-101 -0.1912111858502641 1104 102-103 -0.17063763390791564 1104 104-105 -0.11863011575696092 1104 106-107 -0.11390572548742028 1104 108-109 -0.12709200346465366 1104 110-111 -0.07170946627036301 1104 112-113 -0.12567687490192014 1104 114-115 -0.008904155767638144 1104 116-117 -0.01549587355384574 1104 118-119 -0.07782687587794257 1104 120-121 0.0033305038353894645 1104 122-123 0.02424996838726301 1104 124-125 0.026710641074760844 1105 1 0.13844708906452396 1105 2 0.5297764560591496 1105 3 0.16475717955565727 1105 4 0.1840041287217531 1105 5 0.30835283971968863 1105 6 0.3000876975973412 1105 7 0.3667562391598551 1105 8 0.22495028700343767 1105 9 0.35972923716768435 1105 10-11 0.17441819297646077 1105 12-13 0.12351878489551638 1105 14-15 0.23656116835147856 1105 16-17 0.3840282560047541 1105 18-19 0.3233236850815331 1105 20-21 0.19730540922209627 1105 22-23 0.2578167230875579 1105 24-25 0.19085921152310448 1105 26-27 0.24830405051617532 1105 28-29 0.11228245437304452 1105 30-31 0.2229587157937729 1105 32-33 0.027584727508262574 1105 34-35 0.19628175078433685 1105 36-37 0.1795087226949974 1105 38-39 0.02256215605274292 1105 40-41 0.0726117671875599 1105 42-43 -0.055814387608201343 1105 44-45 -0.007224973714819782 1105 46-47 -0.015044314038256346 1105 48-49 -0.005095425499547446 1105 50-51 -0.04577632438620327 1105 52-53 -0.14653924111692618 1105 54-55 0.008200605293652075 1105 56-57 0.005742194261557643 1105 58-59 0.0035999049930737215 1105 60-61 -0.08718197730650701 1105 62-63 -0.06367589978811594 1105 64-65 -0.034349308017681324 1105 66-67 0.013205544551745163 1105 68-69 -0.00907281657250536 1105 70-71 -0.006755557748469698 1105 72-73 -0.02306863053426156 1105 74-75 0.038487141499217614 1105 76-77 0.05072294004101252 1105 78-79 -0.03979589597523869 1105 80-81 0.06291332250197001 1105 82-83 0.02451010213973248 1105 84-85 -0.05840285866097261 1105 86-87 -0.04882702833105057 1105 88-89 0.053213362312483525 1105 90-91 0.0977984262804128 1105 92-93 0.05033495659251486 1105 94-95 0.11973618305035671 1105 96-97 0.132870686322196 1105 98-99 -0.043605441826009894 1105 100-101 -0.0942689423431382 1105 102-103 -0.006149450335762197 1105 104-105 -0.03055483226183142 1105 106-107 0.037715172698362665 1105 108-109 0.02502165895435482 1105 110-111 0.006907590972648592 1105 112-113 -0.044713690503620285 1105 114-115 0.0185360884665009 1105 116-117 0.08040479066742634 1105 118-119 0.04358352034161683 1105 120-121 0.08961190691068666 1105 122-123 0.1451454850843028 1105 124-125 0.2572449922197997 1106 1 0.02401311958747243 1106 2 0.39777085395724754 1106 3 0.19063677385897293 1106 4 0.18030407813878213 1106 5 0.22801832375415643 1106 6 0.13684242791516965 1106 7 0.1800501424182812 1106 8 0.0648354398637494 1106 9 -0.04748797542205807 1106 10-11 0.06806167569749277 1106 12-13 0.07971021130995126 1106 14-15 0.020009473720719484 1106 16-17 0.08628583117160815 1106 18-19 0.0017590358432855169 1106 20-21 -0.046550329565221205 1106 22-23 -0.0974962356212501 1106 24-25 -0.13346939955157922 1106 26-27 -0.09665003240039738 1106 28-29 -0.10942601961622245 1106 30-31 -0.022210585254896387 1106 32-33 -0.09421655787640049 1106 34-35 -0.12525910066021595 1106 36-37 -0.06643008254436467 1106 38-39 -0.20664945848578498 1106 40-41 -0.13193381394190595 1106 42-43 -0.047348449086513256 1106 44-45 0.025504587651333566 1106 46-47 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64-65 -0.6877699614383346 2216 66-67 -0.8456763143301131 2216 68-69 -0.6464798539795424 2216 70-71 -0.49079929179220017 2216 72-73 -0.5854950929607199 2216 74-75 -0.6601642071521283 2216 76-77 -0.5231531838351131 2216 78-79 -0.638437994617334 2216 80-81 -0.5774278178391725 2216 82-83 -0.7012197235900928 2216 84-85 -0.6523496526077679 2216 86-87 -0.5613478408518624 2216 88-89 -0.7037888946897723 2216 90-91 -0.6044400759580881 2216 92-93 -0.6323878761303163 2216 94-95 -0.6337566204424476 2216 96-97 -0.7040257505742424 2216 98-99 -0.7039321521527171 2216 100-101 -0.7968293449035428 2216 102-103 -0.7958042399905558 2216 104-105 -0.6658917175987149 2216 106-107 -0.5184656834824928 2216 108-109 -0.5591793252466317 2216 110-111 -0.6007292166641598 2216 112-113 -0.39545554124547166 2216 114-115 -0.6120666421362309 2216 116-117 -0.7224812122185718 2216 118-119 -0.6669984902403954 2216 120-121 -0.7434827761839955 2216 122-123 -0.730118140179318 2216 124-125 -0.9440777591029494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67.0 3 50.0 4 121.0 5 280.0 6 722.0 7 1429.0 8 2250.0 9 3737.0 10 6427.0 11 8310.0 12 8978.0 13 11113.0 14 15258.0 15 11499.0 16 5563.0 17 4791.0 18 4567.0 19 4429.0 20 4414.0 21 4468.0 22 5138.0 23 5863.0 24 7503.0 25 9332.0 26 12077.0 27 15389.0 28 19748.0 29 24757.0 30 31109.0 31 38589.0 32 48575.0 33 62159.0 34 83983.0 35 119226.0 36 194592.0 37 324056.0 38 138811.0 39 632.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.33755969548243 18.27226465979022 10.665619759749388 21.72455588497796 2 19.17329765623047 21.919644261220263 36.29895218671447 22.608105895834797 3 17.986050123748903 28.083702483284423 32.30108442586396 21.62916296710271 4 11.34563430940073 16.272288126708656 37.14679720324836 35.23528036064225 5 9.957425381444697 42.721806015964674 35.48775213115177 11.833016471438853 6 30.07454766566775 37.590684606278 18.010874088315273 14.323893639738971 7 26.07394121992368 35.150385641429274 22.636555130111642 16.1391180085354 8 27.533362580362127 33.143550223707514 20.43762479717938 18.88546239875098 9 27.196188423982992 11.04521569146105 19.91488792043948 41.843707964116476 10-11 23.991098473240584 26.00571607371542 30.19216749515327 19.81101795789073 12-13 25.30511801498695 25.550034999661293 30.071926723289778 19.07292026206198 14-15 22.439903807382507 26.576799256781385 28.359483617900473 22.62381331793563 16-17 20.895080047612442 28.729802614813405 28.69923839446715 21.675878943107 18-19 20.033072260591027 26.51526759418457 32.56045102789328 20.891209117331123 20-21 21.23265742589588 25.78648432434525 32.92972164785502 20.051136601903853 22-23 21.06971545436657 26.21660112966649 32.522427202317395 20.191256213649545 24-25 20.499527625348634 26.168547630405044 33.00146813154555 20.33045661270077 26-27 20.518430466801934 26.149585649171136 32.78319080783089 20.548793076196038 28-29 20.460737662513615 26.021331253782726 33.02654854410624 20.491382539597417 30-31 20.59000235481592 25.799669680615995 33.1875417334671 20.42278623110099 32-33 20.371052860778764 26.19180298255178 32.86689967516443 20.570244481505018 34-35 20.819025653018926 26.204949960080963 32.48776219546616 20.48826219143394 36-37 21.024756212036657 25.872048012438587 32.16218875301207 20.941007022512686 38-39 20.747137930923248 26.144585697557766 32.32783231130021 20.780444060218773 40-41 20.94253926574904 26.181157924278153 32.25367980309868 20.62262300687413 42-43 21.030950449280716 26.348769578913245 31.72142821428053 20.898851757525513 44-45 20.98638776814645 26.543872277704857 31.32059417160163 21.149145782547066 46-47 21.158226265656882 26.774984474178744 30.692451627993258 21.374337632171112 48-49 21.538203451987243 26.879383689499388 30.026828895522968 21.555583962990397 50-51 21.4601433568901 26.94419969151049 30.044902362061837 21.55075458953757 52-53 21.366439489955283 27.138254926384985 30.04293385798639 21.452371725673334 54-55 21.204544968810502 27.243036952772375 30.017206748335884 21.535211330081243 56-57 21.36222648046531 27.396431140660738 29.9217789778281 21.319563401045848 58-59 21.58460029742607 26.80193585016436 29.850472430022613 21.762991422386957 60-61 21.497757033841065 27.027288581538123 30.15986048246454 21.31509390215627 62-63 21.356330237348114 26.973499043946212 30.231340015629122 21.43883070307655 64-65 21.231539706893386 27.04722004366962 30.201674606902863 21.51956564253413 66-67 21.259493581562644 27.35857159274425 29.87864748091203 21.503287344781075 68-69 21.337848392558538 27.18325868942583 29.84991514102638 21.62897777698925 70-71 21.30947446180844 27.06893464926217 30.01808084929991 21.60351003962948 72-73 21.56837681624595 27.252459497624574 29.493447119288962 21.68571656684051 74-75 21.15622207670298 27.45341950509837 29.635630207644493 21.75472821055416 76-77 21.080574980856955 27.54372658530419 29.729199000166535 21.64649943367232 78-79 21.314174355781663 27.395090606087606 29.519024234068986 21.77171080406174 80-81 21.113236099972056 27.33872765989533 29.436942467640293 22.11109377249232 82-83 21.258538237594856 27.442722236111 29.333029572342156 21.965709953951983 84-85 21.02948446341922 27.193512305152623 29.550108104054086 22.226895127374075 86-87 21.020331304046987 27.229197530017927 29.426117068505146 22.32435409742994 88-89 21.3757113415863 26.96386326273782 29.45185014336711 22.208575252308773 90-91 21.0424850543029 27.274314530209544 29.429132032622867 22.254068382864688 92-93 21.18341171992439 27.25651769988581 29.327633370107293 22.232437210082516 94-95 20.963380586097717 27.03701941454744 29.2712972117697 22.728302787585143 96-97 21.22196361923748 27.33194300626776 29.10860196323214 22.337491411262615 98-99 21.11669805120182 27.111802870172863 29.005439538062657 22.766059540562665 100-101 21.015658014006405 27.000316128012493 29.334018277360396 22.650007580620706 102-103 20.95983870967742 26.966250000000002 29.034274193548388 23.039637096774193 104-105 20.995795977912955 26.719048260367114 29.34004998375004 22.945105777969893 106-107 20.9867129675179 26.675516086212486 29.32937421270542 23.008396733564194 108-109 21.126498183067447 26.897537262036675 28.82991317755939 23.146051377336487 110-111 21.11856316302843 26.85516912681154 28.812868371530197 23.21339933862983 112-113 21.012799320574203 26.761423874509475 28.968105943322307 23.25767086159401 114-115 20.638259772566318 27.04344276972864 28.748146797811806 23.570150659893237 116-117 20.58014676079684 27.00362364869361 28.891107037783144 23.52512255272641 118-119 20.59171645357266 27.059181727079707 28.609065081551055 23.74003673779658 120-121 20.5386147716825 27.06993886800974 28.554567156475315 23.836879203832442 122-123 20.55477056880355 26.877193661608338 28.476792350572932 24.091243419015175 124-125 20.599032702151458 26.91473600930361 28.38540133505491 24.10082995349002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 79.0 1 105.5 2 547.5 3 1038.5 4 820.0 5 800.0 6 1291.5 7 2699.5 8 3936.0 9 4316.5 10 3608.5 11 2373.5 12 2127.0 13 2135.0 14 2270.5 15 2397.0 16 2582.0 17 2790.5 18 2958.5 19 3102.5 20 3437.5 21 3977.5 22 4517.0 23 5562.5 24 6989.0 25 8292.5 26 10096.0 27 12018.5 28 13489.0 29 15192.5 30 17884.5 31 20878.0 32 23525.0 33 26025.0 34 29616.0 35 31945.0 36 33941.5 37 36697.0 38 37300.5 39 37455.0 40 37087.5 41 37777.5 42 39735.0 43 41750.5 44 44819.5 45 46740.5 46 46033.5 47 45469.0 48 45357.5 49 43501.5 50 41536.5 51 38897.5 52 36126.0 53 34747.0 54 33273.0 55 30934.0 56 27567.0 57 24774.0 58 22702.0 59 20201.0 60 17352.5 61 14224.5 62 11483.5 63 9262.0 64 7036.0 65 5311.0 66 4105.5 67 3171.0 68 2326.5 69 1489.0 70 982.5 71 780.5 72 728.5 73 657.0 74 447.0 75 343.5 76 227.5 77 134.5 78 84.0 79 30.0 80 12.5 81 4.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06499937097382928 2 8.064438086083038E-5 3 8.064438086083038E-5 4 1.6128876172166075E-4 5 0.06258003954800437 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.032219043041519E-5 26-27 0.0 28-29 4.032219043041519E-5 30-31 0.0 32-33 0.0 34-35 1.6128876172166075E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002419331425824911 44-45 0.010846669225781685 46-47 0.011451502082237913 48-49 0.00947571475114757 50-51 0.008024115895652623 52-53 0.006572517040157676 54-55 0.007378960848765981 56-57 0.005161240375093144 58-59 0.003225775234433215 60-61 0.001975787331090344 62-63 0.0015322432363557772 64-65 0.0015725654267861923 66-67 0.002379009235394496 68-69 0.0010886991416212102 70-71 0.00209675390238159 72-73 0.001854820759799099 74-75 6.45155046886643E-4 76-77 4.435440947345671E-4 78-79 1.2096657129124556E-4 80-81 4.435440947345671E-4 82-83 1.6128876172166075E-4 84-85 2.4193314258249112E-4 86-87 2.4193314258249112E-4 88-89 0.0010886991416212102 90-91 0.001854820759799099 92-93 3.225775234433215E-4 94-95 0.0011290213320516254 96-97 0.001290310093773286 98-99 0.0013709544746341165 100-101 6.45155046886643E-4 102-103 9.677325703299645E-4 104-105 7.257994277474735E-4 106-107 4.8386628516498224E-4 108-109 8.064438086083038E-4 110-111 8.46765999038719E-4 112-113 0.011411179891807498 114-115 0.021451405308980882 116-117 0.019314329216168876 118-119 0.01165311303438999 120-121 0.005806395421979788 122-123 0.006128972945423109 124-125 0.005080595994232314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1240012.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.103980097146806 #Duplication Level Percentage of deduplicated Percentage of total 1 42.20869254097268 11.440235625571123 2 16.273724040674324 8.821653850097928 3 12.004751624196327 9.761296472802242 4 8.756961325757679 9.493940219392819 5 6.0681560282841485 8.22355901084975 6 3.956182746751925 6.433697905718384 7 2.636829335258809 5.002799887569933 8 1.7762666598461412 3.8515116956556197 9 1.2271436152966888 2.9934428512808005 >10 4.70554283976887 21.362498732880148 >50 0.2656001906740835 4.986646500816521 >100 0.11564346382262355 5.065431328121293 >500 0.00210260805806631 0.34209710759602985 >1k 0.001501862898618793 0.591139242802183 >5k 9.011177391712758E-4 1.6300495688452425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8007 0.6457195575526689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6284 0.5067692893294581 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5731 0.46217294671341885 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.13862769069976744 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.13677286993996832 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 0.12854714309216364 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.032219043041519E-5 0.0 12-13 0.0 0.0 0.0 8.064438086083038E-5 0.0 14-15 0.0 0.0 0.0 8.064438086083038E-5 0.0 16-17 0.0 0.0 0.0 8.064438086083038E-5 0.0 18-19 0.0 0.0 0.0 8.064438086083038E-5 0.0 20-21 0.0 0.0 0.0 8.064438086083038E-5 0.0 22-23 0.0 0.0 0.0 1.2096657129124556E-4 0.0 24-25 0.0 0.0 0.0 3.6289971387373673E-4 0.0 26-27 0.0 0.0 0.0 6.854772373170582E-4 0.0 28-29 0.0 0.0 0.0 0.001048376951190795 0.0 30-31 0.0 0.0 0.0 0.003145130853572385 0.0 32-33 0.0 0.0 0.0 0.007983793705222206 0.0 34-35 0.0 0.0 0.0 0.015443398934849018 0.0 36-37 0.0 0.0 0.0 0.029596487775924746 0.0 38-39 0.0 0.0 0.0 0.05221723660738767 0.0 40-41 0.0 0.0 0.0 0.07568475143788932 0.0 42-43 0.0 0.0 0.0 0.10524091702338365 0.0 44-45 0.0 0.0 0.0 0.14479698583562095 0.0 46-47 0.0 0.0 0.0 0.19011912787940763 0.0 48-49 0.0 0.0 0.0 0.24015896620355287 0.0 50-51 0.0 0.0 0.0 0.2841504759631358 0.0 52-53 0.0 0.0 0.0 0.3348757915245981 0.0 54-55 0.0 0.0 0.0 0.4021332051625307 0.0 56-57 0.0 0.0 0.0 0.4831404857372348 0.0 58-59 0.0 0.0 0.0 0.5804379312458267 0.0 60-61 0.0 0.0 0.0 0.6793482643716351 0.0 62-63 0.0 0.0 0.0 0.7828150050160805 0.0 64-65 0.0 0.0 0.0 0.9000316125972974 0.0 66-67 0.0 0.0 0.0 1.0437802214817276 0.0 68-69 0.0 0.0 0.0 1.2213188259468457 0.0 70-71 0.0 0.0 0.0 1.4068815463076163 0.0 72-73 0.0 0.0 0.0 1.5868798043889898 0.0 74-75 0.0 0.0 0.0 1.7872810908281531 0.0 76-77 0.0 0.0 0.0 2.0219965613236 0.0 78-79 0.0 0.0 0.0 2.296510033773867 0.0 80-81 0.0 0.0 0.0 2.5831604855436883 0.0 82-83 0.0 0.0 0.0 2.8774721534952885 0.0 84-85 0.0 0.0 0.0 3.1854127218123693 0.0 86-87 0.0 0.0 0.0 3.5379496327454896 0.0 88-89 0.0 0.0 0.0 3.937623184291765 0.0 90-91 0.0 0.0 0.0 4.360119095621656 0.0 92-93 0.0 0.0 0.0 4.78886494646826 0.0 94-95 0.0 0.0 0.0 5.2468040631864845 0.0 96-97 0.0 0.0 0.0 5.725226852643361 0.0 98-99 0.0 0.0 0.0 6.2709473779286 0.0 100-101 0.0 0.0 0.0 6.86618355306239 0.0 102-103 0.0 0.0 0.0 7.445653751737886 0.0 104-105 0.0 0.0 0.0 8.059196201327083 0.0 106-107 0.0 0.0 0.0 8.71338341887014 0.0 108-109 0.0 0.0 0.0 9.414183088550757 0.0 110-111 0.0 0.0 0.0 10.131756789450424 0.0 112-113 0.0 0.0 0.0 10.855983651771112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4010 0.0 83.50081 1 AGCGTAC 55 1.3069566E-5 54.11752 3 GTATCAA 6225 0.0 53.743694 2 GTACATG 9905 0.0 50.46685 1 TACATGG 10130 0.0 49.362873 2 ACATGGG 10250 0.0 49.07535 3 CATGGGA 7460 0.0 46.602005 4 CATGGGG 2835 0.0 40.10614 4 ATCAACG 8750 0.0 39.459404 4 ATGGGAT 3230 0.0 39.256145 5 TCAACGC 8840 0.0 39.12501 5 ATGGGAG 2840 0.0 38.777874 5 CAACGCA 8995 0.0 38.625977 6 AACGCAG 9120 0.0 38.1618 7 TATCAAC 9130 0.0 37.882263 3 CCGAGTA 200 5.0931703E-11 32.7411 4 ACGCAGA 10645 0.0 32.694748 8 CGCAGAG 10745 0.0 32.169 9 AGTACAT 5985 0.0 31.828514 2 TACCTGG 875 0.0 31.29539 2 >>END_MODULE