FastQCFastQC Report
Thu 26 May 2016
SRR936260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374367
Sequences flagged as poor quality0
Sequence length125
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75162.0076555893014074No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37691.0067660878229117No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19690.5259544778252356No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT18960.5064548958642188No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG15840.42311421679795497No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15310.4089569860591345No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14320.3825123475092623No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT12360.33015730553173755No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT11940.3189383679651251No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA11670.31172619381516No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11030.2946306699041315No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC10760.2874184957541664No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA10700.2858157903875075No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10460.27940496892087174No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9320.24895356695435228No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT9290.2481522142710228No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG9080.2425427454877166No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC8460.22598145669890773No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA8230.2198377527933819No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7740.2067489922990007No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7710.20594763961567125No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.20461205181012215No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT7640.2040778166879025No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7420.1982012303434865No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT7020.18751652789909368No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6950.18564670497132493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6940.1853795874102151No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6880.1837768820435562No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT6680.1784345308213598No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT6420.17148947423250446No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6410.17122235667139465No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.16961965130473572No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6140.16401018252142952No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG6040.16133900691033132No Hit
GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT5810.15519530300480547No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC5650.15092142202704834No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG5640.1506543044659385No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT5590.1493187166603894No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT5560.14851736397705995No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG5550.14825024641595014No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5550.14825024641595014No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA5550.14825024641595014No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG5540.1479831288548403No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC5470.14611330592707156No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5450.14557907080485194No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5440.1453119532437421No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT5400.14424348299930284No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA5340.1426407776326439No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA5270.14077095470487516No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA5230.13970248446043587No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA5130.13703130884933767No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG5080.13569572104378858No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT5070.13542860348267877No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC5050.13489436836045912No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC5030.13436013323823948No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT4960.13249031031047073No Hit
GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT4830.12901778201604308No Hit
GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA4830.12901778201604308No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG4720.12607948884383507No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA4720.12607948884383507No Hit
GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT4550.12153849030496812No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.12047002006052884No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG4450.11886731469386992No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT4420.11806596201054047No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC4420.11806596201054047No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG4410.11779884444943065No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT4380.11699749176610116No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG4350.11619613908277172No Hit
CAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTAC4150.11085378786057531No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT4120.11005243517724586No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC4090.1092510824939164No Hit
GTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAA4080.10898396493280657No Hit
CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT4050.10818261224947712No Hit
GGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGG3960.10577855419948874No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG3950.10551143663837892No Hit
GGCTTATATTATGGATCATATACATTTATAGAAACCTGAAACATTGGAGT3940.1052443190772691No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA3930.10497720151615927No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC3930.10497720151615927No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA3860.10310737858839054No Hit
ATGTAATGGTAGCTGTTGGTGGGCTAATATTTATTAATACTAGAGTAGCT3850.10284026102728072No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT3790.1012375556606218No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC3770.10070332053840214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG358.952811E-784.989992
GGGACCT454.2942702E-879.334587
GGACCTA304.2601165E-579.334588
TGGGTAT1550.072.926896
GAACAAA5400.072.713651
CCCTGGC250.002055632471.401127
TGGGACC508.92087E-871.391596
GAGAACA2300.069.839591
GGACTGG701.7462298E-1067.991996
CATGGGT2450.067.991984
ACCTGGG453.9864117E-666.103323
GTATGGT453.9864117E-666.103321
CTTAACG150.00421334959.5805220-21
GGTACTC1300.059.492993
TGGACTG805.7298166E-1059.492995
GATCCTC401.7714636E-459.492995
GCCTCTC150.00423795359.4929932-33
TACAGGG300.004236359.492992
GTGGTAC1300.059.492991
TACAAAG300.004236359.492992
Produced by FastQC (version 0.11.5)